CA2618694A1 - Genes issus de d'actinobacillus succinogenes 130z (atcc 55618) pour la production d'agents chimiques issus de la voie c4 d'a. succinogenes - Google Patents
Genes issus de d'actinobacillus succinogenes 130z (atcc 55618) pour la production d'agents chimiques issus de la voie c4 d'a. succinogenes Download PDFInfo
- Publication number
- CA2618694A1 CA2618694A1 CA002618694A CA2618694A CA2618694A1 CA 2618694 A1 CA2618694 A1 CA 2618694A1 CA 002618694 A CA002618694 A CA 002618694A CA 2618694 A CA2618694 A CA 2618694A CA 2618694 A1 CA2618694 A1 CA 2618694A1
- Authority
- CA
- Canada
- Prior art keywords
- succinogenes
- genetically engineered
- pyruvate
- pathway
- enzymes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 241000948980 Actinobacillus succinogenes Species 0.000 title claims abstract description 357
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 156
- 239000000126 substance Substances 0.000 title claims abstract description 64
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 41
- 230000004048 modification Effects 0.000 claims abstract description 111
- 102000004190 Enzymes Human genes 0.000 claims abstract description 107
- 108090000790 Enzymes Proteins 0.000 claims abstract description 107
- 238000000034 method Methods 0.000 claims abstract description 82
- 238000003209 gene knockout Methods 0.000 claims abstract description 63
- 238000012239 gene modification Methods 0.000 claims abstract description 62
- 230000004907 flux Effects 0.000 claims abstract description 50
- KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 claims abstract description 43
- 239000000758 substrate Substances 0.000 claims abstract description 35
- KPGXRSRHYNQIFN-UHFFFAOYSA-N 2-oxoglutaric acid Chemical compound OC(=O)CCC(=O)C(O)=O KPGXRSRHYNQIFN-UHFFFAOYSA-N 0.000 claims abstract description 27
- ZGXJTSGNIOSYLO-UHFFFAOYSA-N 88755TAZ87 Chemical compound NCC(=O)CCC(O)=O ZGXJTSGNIOSYLO-UHFFFAOYSA-N 0.000 claims abstract description 26
- 229940049920 malate Drugs 0.000 claims abstract description 25
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 claims abstract description 24
- VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 claims abstract description 23
- 229940009098 aspartate Drugs 0.000 claims abstract description 23
- 229930195712 glutamate Natural products 0.000 claims abstract description 22
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 claims abstract description 20
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 claims abstract description 19
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 claims description 65
- 239000001963 growth medium Substances 0.000 claims description 56
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 claims description 53
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 claims description 53
- 238000012258 culturing Methods 0.000 claims description 52
- 238000012986 modification Methods 0.000 claims description 49
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 claims description 45
- 108010026217 Malate Dehydrogenase Proteins 0.000 claims description 38
- 101710088194 Dehydrogenase Proteins 0.000 claims description 36
- 108020005115 Pyruvate Kinase Proteins 0.000 claims description 36
- 102000013009 Pyruvate Kinase Human genes 0.000 claims description 36
- 108010008221 formate C-acetyltransferase Proteins 0.000 claims description 36
- 108010036781 Fumarate Hydratase Proteins 0.000 claims description 35
- 102100036160 Fumarate hydratase, mitochondrial Human genes 0.000 claims description 35
- 102000019259 Succinate Dehydrogenase Human genes 0.000 claims description 34
- 108010012901 Succinate Dehydrogenase Proteins 0.000 claims description 34
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims description 31
- 229930091371 Fructose Natural products 0.000 claims description 30
- 239000005715 Fructose Substances 0.000 claims description 30
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 30
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 29
- 239000008103 glucose Substances 0.000 claims description 29
- 102000016862 Dicarboxylic Acid Transporters Human genes 0.000 claims description 25
- 108010092943 Dicarboxylic Acid Transporters Proteins 0.000 claims description 24
- 102000011929 Succinate-CoA Ligases Human genes 0.000 claims description 23
- 108010075728 Succinate-CoA Ligases Proteins 0.000 claims description 23
- 108010017192 4-hydroxy-4-methyl-2-oxoglutarate aldolase Proteins 0.000 claims description 22
- 102100029589 Acylpyruvase FAHD1, mitochondrial Human genes 0.000 claims description 22
- 108010069823 Oxaloacetate decarboxylase Proteins 0.000 claims description 22
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 claims description 22
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 20
- 229930006000 Sucrose Natural products 0.000 claims description 19
- 239000005720 sucrose Substances 0.000 claims description 19
- 101710104378 Putative malate oxidoreductase [NAD] Proteins 0.000 claims description 18
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 17
- 108090000472 Phosphoenolpyruvate carboxykinase (ATP) Proteins 0.000 claims description 15
- 239000004471 Glycine Substances 0.000 claims description 14
- 230000029142 excretion Effects 0.000 claims description 14
- 102000006589 Alpha-ketoglutarate dehydrogenase Human genes 0.000 claims description 12
- 108020004306 Alpha-ketoglutarate dehydrogenase Proteins 0.000 claims description 12
- 101000950981 Bacillus subtilis (strain 168) Catabolic NAD-specific glutamate dehydrogenase RocG Proteins 0.000 claims description 9
- 102000016901 Glutamate dehydrogenase Human genes 0.000 claims description 9
- 102000004316 Oxidoreductases Human genes 0.000 claims description 8
- 108090000854 Oxidoreductases Proteins 0.000 claims description 8
- 230000015572 biosynthetic process Effects 0.000 claims description 8
- 238000003786 synthesis reaction Methods 0.000 claims description 8
- 102100032948 Aspartoacylase Human genes 0.000 claims description 7
- 101000797251 Homo sapiens Aspartoacylase Proteins 0.000 claims description 7
- 108010003415 Aspartate Aminotransferases Proteins 0.000 claims description 6
- 102000004625 Aspartate Aminotransferases Human genes 0.000 claims description 6
- 125000005478 oxoglutarate group Chemical group 0.000 claims description 4
- 102100037579 D-3-phosphoglycerate dehydrogenase Human genes 0.000 claims 14
- 102100034792 Phosphoenolpyruvate carboxykinase [GTP], mitochondrial Human genes 0.000 claims 14
- BJEPYKJPYRNKOW-UHFFFAOYSA-L malate(2-) Chemical compound [O-]C(=O)C(O)CC([O-])=O BJEPYKJPYRNKOW-UHFFFAOYSA-L 0.000 claims 4
- CKLJMWTZIZZHCS-REOHCLBHSA-L aspartate group Chemical group N[C@@H](CC(=O)[O-])C(=O)[O-] CKLJMWTZIZZHCS-REOHCLBHSA-L 0.000 claims 3
- WHUUTDBJXJRKMK-VKHMYHEASA-L glutamate group Chemical group N[C@@H](CCC(=O)[O-])C(=O)[O-] WHUUTDBJXJRKMK-VKHMYHEASA-L 0.000 claims 3
- 150000003890 succinate salts Chemical group 0.000 claims 3
- 230000037361 pathway Effects 0.000 abstract description 55
- BJEPYKJPYRNKOW-UHFFFAOYSA-N malic acid Chemical compound OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 abstract description 21
- 102000003939 Membrane transport proteins Human genes 0.000 description 26
- 108090000301 Membrane transport proteins Proteins 0.000 description 26
- 235000018102 proteins Nutrition 0.000 description 26
- 102000004169 proteins and genes Human genes 0.000 description 26
- 102000013460 Malate Dehydrogenase Human genes 0.000 description 24
- 108700026244 Open Reading Frames Proteins 0.000 description 23
- KDYFGRWQOYBRFD-UHFFFAOYSA-N succinic acid Chemical compound OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 description 20
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 17
- 238000012824 chemical production Methods 0.000 description 17
- 102000007382 Ribose-5-phosphate isomerase Human genes 0.000 description 16
- 108020005610 ribose 5-phosphate isomerase Proteins 0.000 description 16
- 235000000346 sugar Nutrition 0.000 description 14
- 241001293415 Mannheimia Species 0.000 description 13
- 102000014701 Transketolase Human genes 0.000 description 12
- 108010043652 Transketolase Proteins 0.000 description 12
- 102000005924 Triose-Phosphate Isomerase Human genes 0.000 description 12
- 108700015934 Triose-phosphate isomerases Proteins 0.000 description 12
- -1 PEPCK Proteins 0.000 description 10
- 102100029089 Xylulose kinase Human genes 0.000 description 10
- 108091022915 xylulokinase Proteins 0.000 description 10
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 9
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 9
- 229910052799 carbon Inorganic materials 0.000 description 9
- IKHGUXGNUITLKF-UHFFFAOYSA-N Acetaldehyde Chemical compound CC=O IKHGUXGNUITLKF-UHFFFAOYSA-N 0.000 description 8
- BDAGIHXWWSANSR-UHFFFAOYSA-M Formate Chemical compound [O-]C=O BDAGIHXWWSANSR-UHFFFAOYSA-M 0.000 description 8
- 108030003782 L-xylulokinases Proteins 0.000 description 8
- 108020004530 Transaldolase Proteins 0.000 description 8
- 102100028601 Transaldolase Human genes 0.000 description 8
- ZSLZBFCDCINBPY-ZSJPKINUSA-N acetyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 ZSLZBFCDCINBPY-ZSJPKINUSA-N 0.000 description 8
- 238000013459 approach Methods 0.000 description 8
- 230000002401 inhibitory effect Effects 0.000 description 8
- 238000002372 labelling Methods 0.000 description 8
- 108010023317 1-phosphofructokinase Proteins 0.000 description 7
- 102100031126 6-phosphogluconolactonase Human genes 0.000 description 7
- 108010029731 6-phosphogluconolactonase Proteins 0.000 description 7
- 241000417230 Actinobacillus succinogenes 130Z Species 0.000 description 7
- 241000588724 Escherichia coli Species 0.000 description 7
- 108091000080 Phosphotransferase Proteins 0.000 description 7
- 229940024606 amino acid Drugs 0.000 description 7
- 150000001413 amino acids Chemical class 0.000 description 7
- 230000034659 glycolysis Effects 0.000 description 7
- 102000020233 phosphotransferase Human genes 0.000 description 7
- 239000000047 product Substances 0.000 description 7
- 102000004567 6-phosphogluconate dehydrogenase Human genes 0.000 description 6
- 108020001657 6-phosphogluconate dehydrogenase Proteins 0.000 description 6
- 108010021809 Alcohol dehydrogenase Proteins 0.000 description 6
- 101000597583 Neisseria gonorrhoeae Outer membrane protein P.IIC Proteins 0.000 description 6
- 108010022181 Phosphopyruvate Hydratase Proteins 0.000 description 6
- 102000012288 Phosphopyruvate Hydratase Human genes 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
- 239000012528 membrane Substances 0.000 description 6
- 230000002503 metabolic effect Effects 0.000 description 6
- 230000004060 metabolic process Effects 0.000 description 6
- 244000005700 microbiome Species 0.000 description 6
- VNOYUJKHFWYWIR-ITIYDSSPSA-N succinyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)CCC(O)=O)O[C@H]1N1C2=NC=NC(N)=C2N=C1 VNOYUJKHFWYWIR-ITIYDSSPSA-N 0.000 description 6
- 108010092060 Acetate kinase Proteins 0.000 description 5
- 102000007698 Alcohol dehydrogenase Human genes 0.000 description 5
- 102000005369 Aldehyde Dehydrogenase Human genes 0.000 description 5
- 108020002663 Aldehyde Dehydrogenase Proteins 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 5
- 108010021582 Glucokinase Proteins 0.000 description 5
- 102000030595 Glucokinase Human genes 0.000 description 5
- 108060007030 Ribulose-phosphate 3-epimerase Proteins 0.000 description 5
- 108050005268 Sugar efflux Proteins 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 5
- KHPXUQMNIQBQEV-UHFFFAOYSA-N oxaloacetic acid Chemical compound OC(=O)CC(=O)C(O)=O KHPXUQMNIQBQEV-UHFFFAOYSA-N 0.000 description 5
- 239000013612 plasmid Substances 0.000 description 5
- 230000019525 primary metabolic process Effects 0.000 description 5
- 102000004688 ribulosephosphate 3-epimerase Human genes 0.000 description 5
- 102000021527 ATP binding proteins Human genes 0.000 description 4
- 108091011108 ATP binding proteins Proteins 0.000 description 4
- 108010029692 Bisphosphoglycerate mutase Proteins 0.000 description 4
- 108010078791 Carrier Proteins Proteins 0.000 description 4
- 108091026890 Coding region Proteins 0.000 description 4
- 108090000156 Fructokinases Proteins 0.000 description 4
- 102000003793 Fructokinases Human genes 0.000 description 4
- 108010043428 Glycine hydroxymethyltransferase Proteins 0.000 description 4
- 108010018080 L-arabinose isomerase Proteins 0.000 description 4
- 108050004064 Major facilitator superfamily Proteins 0.000 description 4
- 102000015841 Major facilitator superfamily Human genes 0.000 description 4
- 102000000818 NADP Transhydrogenases Human genes 0.000 description 4
- 108010001609 NADP Transhydrogenases Proteins 0.000 description 4
- 108010090127 Periplasmic Proteins Proteins 0.000 description 4
- 108700023175 Phosphate acetyltransferases Proteins 0.000 description 4
- 102000011025 Phosphoglycerate Mutase Human genes 0.000 description 4
- 108010034634 Repressor Proteins Proteins 0.000 description 4
- 102000009661 Repressor Proteins Human genes 0.000 description 4
- 102100021225 Serine hydroxymethyltransferase, cytosolic Human genes 0.000 description 4
- 108700040099 Xylose isomerases Proteins 0.000 description 4
- 108010081577 aldehyde dehydrogenase (NAD(P)+) Proteins 0.000 description 4
- 108010051210 beta-Fructofuranosidase Proteins 0.000 description 4
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 4
- 235000011073 invertase Nutrition 0.000 description 4
- 230000000670 limiting effect Effects 0.000 description 4
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 4
- DTBNBXWJWCWCIK-UHFFFAOYSA-K phosphonatoenolpyruvate Chemical compound [O-]C(=O)C(=C)OP([O-])([O-])=O DTBNBXWJWCWCIK-UHFFFAOYSA-K 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 150000008495 β-glucosides Chemical class 0.000 description 4
- 102000005991 Acylphosphatase Human genes 0.000 description 3
- 108700006311 Acylphosphatases Proteins 0.000 description 3
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 3
- 230000004619 Entner-Doudoroff pathway Effects 0.000 description 3
- 108010018962 Glucosephosphate Dehydrogenase Proteins 0.000 description 3
- 102000005298 Iron-Sulfur Proteins Human genes 0.000 description 3
- 108010081409 Iron-Sulfur Proteins Proteins 0.000 description 3
- 229930195725 Mannitol Natural products 0.000 description 3
- 241000606752 Pasteurellaceae Species 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 108091006374 cAMP receptor proteins Proteins 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 230000001419 dependent effect Effects 0.000 description 3
- 101150018523 frdB gene Proteins 0.000 description 3
- 230000001890 gluconeogenic effect Effects 0.000 description 3
- 239000000543 intermediate Substances 0.000 description 3
- 239000001573 invertase Substances 0.000 description 3
- 239000000594 mannitol Substances 0.000 description 3
- 235000010355 mannitol Nutrition 0.000 description 3
- 230000035772 mutation Effects 0.000 description 3
- 230000001590 oxidative effect Effects 0.000 description 3
- 101150088738 pckA gene Proteins 0.000 description 3
- 101150067708 pckG gene Proteins 0.000 description 3
- 108010038136 phospho-2-keto-3-deoxy-gluconate aldolase Proteins 0.000 description 3
- 229930029653 phosphoenolpyruvate Natural products 0.000 description 3
- 108010022393 phosphogluconate dehydratase Proteins 0.000 description 3
- 239000001384 succinic acid Substances 0.000 description 3
- 150000008163 sugars Chemical class 0.000 description 3
- OSJPPGNTCRNQQC-UWTATZPHSA-N 3-phospho-D-glyceric acid Chemical compound OC(=O)[C@H](O)COP(O)(O)=O OSJPPGNTCRNQQC-UWTATZPHSA-N 0.000 description 2
- 108030003650 6-phosphofructokinases Proteins 0.000 description 2
- 102100025514 ATP-dependent 6-phosphofructokinase, platelet type Human genes 0.000 description 2
- 101710129685 Arabinose-proton symporter Proteins 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 2
- SRBFZHDQGSBBOR-SOOFDHNKSA-N D-ribopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@@H]1O SRBFZHDQGSBBOR-SOOFDHNKSA-N 0.000 description 2
- ZAQJHHRNXZUBTE-WUJLRWPWSA-N D-xylulose Chemical compound OC[C@@H](O)[C@H](O)C(=O)CO ZAQJHHRNXZUBTE-WUJLRWPWSA-N 0.000 description 2
- 102100031920 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial Human genes 0.000 description 2
- 108700035160 EC 4.2.1.14 Proteins 0.000 description 2
- 108050008991 Fructose-1,6-bisphosphatase class 1 Proteins 0.000 description 2
- 102100030892 Fructose-1,6-bisphosphatase isozyme 2 Human genes 0.000 description 2
- 102000012195 Fructose-1,6-bisphosphatases Human genes 0.000 description 2
- 108010017464 Fructose-Bisphosphatase Proteins 0.000 description 2
- 108010068561 Fructose-Bisphosphate Aldolase Proteins 0.000 description 2
- 102000001390 Fructose-Bisphosphate Aldolase Human genes 0.000 description 2
- 102100035172 Glucose-6-phosphate 1-dehydrogenase Human genes 0.000 description 2
- 108010070600 Glucose-6-phosphate isomerase Proteins 0.000 description 2
- 102000005731 Glucose-6-phosphate isomerase Human genes 0.000 description 2
- 108010023021 Glutamyl-tRNA reductase Proteins 0.000 description 2
- 102100031181 Glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 2
- 102000005548 Hexokinase Human genes 0.000 description 2
- 108700040460 Hexokinases Proteins 0.000 description 2
- 102000003855 L-lactate dehydrogenase Human genes 0.000 description 2
- 108700023483 L-lactate dehydrogenases Proteins 0.000 description 2
- 108090000416 L-ribulose-5-phosphate 4-epimerases Proteins 0.000 description 2
- 108010015328 Mannokinase Proteins 0.000 description 2
- 238000005481 NMR spectroscopy Methods 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 2
- 101710148592 PTS system fructose-like EIIA component Proteins 0.000 description 2
- 101710169713 PTS system fructose-specific EIIA component Proteins 0.000 description 2
- 108010022684 Phosphofructokinase-1 Proteins 0.000 description 2
- 102000012435 Phosphofructokinase-1 Human genes 0.000 description 2
- 102000011755 Phosphoglycerate Kinase Human genes 0.000 description 2
- 108700023219 Phosphoglycerate kinases Proteins 0.000 description 2
- 108010053763 Pyruvate Carboxylase Proteins 0.000 description 2
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 2
- 108010006873 Threonine Dehydratase Proteins 0.000 description 2
- 108030003022 Threonine ammonia-lyases Proteins 0.000 description 2
- XJLXINKUBYWONI-DQQFMEOOSA-N [[(2r,3r,4r,5r)-5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2s,3r,4s,5s)-5-(3-carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate Chemical compound NC(=O)C1=CC=C[N+]([C@@H]2[C@H]([C@@H](O)[C@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](OP(O)(O)=O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 XJLXINKUBYWONI-DQQFMEOOSA-N 0.000 description 2
- LIPOUNRJVLNBCD-UHFFFAOYSA-N acetyl dihydrogen phosphate Chemical compound CC(=O)OP(O)(O)=O LIPOUNRJVLNBCD-UHFFFAOYSA-N 0.000 description 2
- 108010043899 acetylphosphatase Proteins 0.000 description 2
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 2
- 239000011942 biocatalyst Substances 0.000 description 2
- KDYFGRWQOYBRFD-NUQCWPJISA-N butanedioic acid Chemical compound O[14C](=O)CC[14C](O)=O KDYFGRWQOYBRFD-NUQCWPJISA-N 0.000 description 2
- 210000004899 c-terminal region Anatomy 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 239000012847 fine chemical Substances 0.000 description 2
- 239000002803 fossil fuel Substances 0.000 description 2
- 230000037433 frameshift Effects 0.000 description 2
- 238000002290 gas chromatography-mass spectrometry Methods 0.000 description 2
- 230000004110 gluconeogenesis Effects 0.000 description 2
- 102000006602 glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 2
- 101150112623 hemA gene Proteins 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 238000012269 metabolic engineering Methods 0.000 description 2
- 230000037353 metabolic pathway Effects 0.000 description 2
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 description 2
- 150000007524 organic acids Chemical class 0.000 description 2
- 235000005985 organic acids Nutrition 0.000 description 2
- 230000002018 overexpression Effects 0.000 description 2
- 235000004252 protein component Nutrition 0.000 description 2
- 108020000318 saccharopine dehydrogenase Proteins 0.000 description 2
- 230000002103 transcriptional effect Effects 0.000 description 2
- LXJXRIRHZLFYRP-VKHMYHEASA-L (R)-2-Hydroxy-3-(phosphonooxy)-propanal Natural products O=C[C@H](O)COP([O-])([O-])=O LXJXRIRHZLFYRP-VKHMYHEASA-L 0.000 description 1
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- CHUGKEQJSLOLHL-UHFFFAOYSA-N 2,2-Bis(bromomethyl)propane-1,3-diol Chemical compound OCC(CO)(CBr)CBr CHUGKEQJSLOLHL-UHFFFAOYSA-N 0.000 description 1
- 108010030844 2-methylcitrate synthase Proteins 0.000 description 1
- 108050003384 2-oxoglutarate dehydrogenase E1 component Proteins 0.000 description 1
- 102100026936 2-oxoglutarate dehydrogenase, mitochondrial Human genes 0.000 description 1
- 108010052384 5-Aminolevulinate Synthetase Proteins 0.000 description 1
- 102000018727 5-Aminolevulinate Synthetase Human genes 0.000 description 1
- 101150007902 ASPA gene Proteins 0.000 description 1
- 108010009924 Aconitate hydratase Proteins 0.000 description 1
- 102100026608 Aldehyde dehydrogenase family 3 member A2 Human genes 0.000 description 1
- 241000722954 Anaerobiospirillum succiniciproducens Species 0.000 description 1
- 102100034193 Aspartate aminotransferase, mitochondrial Human genes 0.000 description 1
- 102100026189 Beta-galactosidase Human genes 0.000 description 1
- 101710180847 Carbon storage regulator Proteins 0.000 description 1
- 108090000209 Carbonic anhydrases Proteins 0.000 description 1
- 102000003846 Carbonic anhydrases Human genes 0.000 description 1
- 102100034229 Citramalyl-CoA lyase, mitochondrial Human genes 0.000 description 1
- 108010071536 Citrate (Si)-synthase Proteins 0.000 description 1
- 108010036824 Citrate (pro-3S)-lyase Proteins 0.000 description 1
- 102000006732 Citrate synthase Human genes 0.000 description 1
- 108020004705 Codon Proteins 0.000 description 1
- 241000186031 Corynebacteriaceae Species 0.000 description 1
- 102100039868 Cytoplasmic aconitate hydratase Human genes 0.000 description 1
- GSXOAOHZAIYLCY-UHFFFAOYSA-N D-F6P Natural products OCC(=O)C(O)C(O)C(O)COP(O)(O)=O GSXOAOHZAIYLCY-UHFFFAOYSA-N 0.000 description 1
- XPYBSIWDXQFNMH-UHFFFAOYSA-N D-fructose 1,6-bisphosphate Natural products OP(=O)(O)OCC(O)C(O)C(O)C(=O)COP(O)(O)=O XPYBSIWDXQFNMH-UHFFFAOYSA-N 0.000 description 1
- LXJXRIRHZLFYRP-VKHMYHEASA-N D-glyceraldehyde 3-phosphate Chemical compound O=C[C@H](O)COP(O)(O)=O LXJXRIRHZLFYRP-VKHMYHEASA-N 0.000 description 1
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
- FNZLKVNUWIIPSJ-RFZPGFLSSA-N D-xylulose 5-phosphate Chemical compound OCC(=O)[C@@H](O)[C@H](O)COP(O)(O)=O FNZLKVNUWIIPSJ-RFZPGFLSSA-N 0.000 description 1
- 108020004414 DNA Proteins 0.000 description 1
- 108010073112 Dihydrolipoyllysine-residue acetyltransferase Proteins 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 108700033374 EC 4.1.1.3 Proteins 0.000 description 1
- 108010052375 Glutamate Dehydrogenase (NADP+) Proteins 0.000 description 1
- 241000606768 Haemophilus influenzae Species 0.000 description 1
- 241000590002 Helicobacter pylori Species 0.000 description 1
- 101000992065 Homo sapiens Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial Proteins 0.000 description 1
- 102000012011 Isocitrate Dehydrogenase Human genes 0.000 description 1
- 108010075869 Isocitrate Dehydrogenase Proteins 0.000 description 1
- 108020003285 Isocitrate lyase Proteins 0.000 description 1
- 101100277154 Klebsiella pneumoniae oadA gene Proteins 0.000 description 1
- ZAQJHHRNXZUBTE-UCORVYFPSA-N L-ribulose Chemical compound OC[C@H](O)[C@H](O)C(=O)CO ZAQJHHRNXZUBTE-UCORVYFPSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 108020004687 Malate Synthase Proteins 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 102000006746 NADH Dehydrogenase Human genes 0.000 description 1
- 108010086428 NADH Dehydrogenase Proteins 0.000 description 1
- 101150045799 PEPCK gene Proteins 0.000 description 1
- OFOBLEOULBTSOW-UHFFFAOYSA-N Propanedioic acid Natural products OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 description 1
- 102000001253 Protein Kinase Human genes 0.000 description 1
- 108010031852 Pyruvate Synthase Proteins 0.000 description 1
- 102100039895 Pyruvate carboxylase, mitochondrial Human genes 0.000 description 1
- 108050006183 Pyruvate dehydrogenase E1 component Proteins 0.000 description 1
- 241000191043 Rhodobacter sphaeroides Species 0.000 description 1
- 101100277156 Salmonella typhi oadA2 gene Proteins 0.000 description 1
- 101100241861 Salmonella typhi oadB1 gene Proteins 0.000 description 1
- 101100406182 Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) oadG2 gene Proteins 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 102100024241 Succinate-CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial Human genes 0.000 description 1
- 101710195626 Transcriptional activator protein Proteins 0.000 description 1
- 101710115749 Translational regulator CsrA Proteins 0.000 description 1
- 241000606834 [Haemophilus] ducreyi Species 0.000 description 1
- 108091006088 activator proteins Proteins 0.000 description 1
- RNBGYGVWRKECFJ-ZXXMMSQZSA-N alpha-D-fructofuranose 1,6-bisphosphate Chemical compound O[C@H]1[C@H](O)[C@](O)(COP(O)(O)=O)O[C@@H]1COP(O)(O)=O RNBGYGVWRKECFJ-ZXXMMSQZSA-N 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- BGWGXPAPYGQALX-ARQDHWQXSA-N beta-D-fructofuranose 6-phosphate Chemical compound OC[C@@]1(O)O[C@H](COP(O)(O)=O)[C@@H](O)[C@@H]1O BGWGXPAPYGQALX-ARQDHWQXSA-N 0.000 description 1
- 108010005774 beta-Galactosidase Proteins 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 238000005422 blasting Methods 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 230000000911 decarboxylating effect Effects 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 108010079167 dihydrolipoamide succinyltransferase Proteins 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 101150018857 emp gene Proteins 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 101150087653 frdC gene Proteins 0.000 description 1
- 101150023570 frdD gene Proteins 0.000 description 1
- RNBGYGVWRKECFJ-UHFFFAOYSA-N fructose-1,6-phosphate Natural products OC1C(O)C(O)(COP(O)(O)=O)OC1COP(O)(O)=O RNBGYGVWRKECFJ-UHFFFAOYSA-N 0.000 description 1
- 238000003208 gene overexpression Methods 0.000 description 1
- 102000034356 gene-regulatory proteins Human genes 0.000 description 1
- 230000023266 generation of precursor metabolites and energy Effects 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000012268 genome sequencing Methods 0.000 description 1
- 229940047650 haemophilus influenzae Drugs 0.000 description 1
- 229940037467 helicobacter pylori Drugs 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000009655 industrial fermentation Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 description 1
- 239000011976 maleic acid Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 101150004568 oadB gene Proteins 0.000 description 1
- 101150055522 oadB2 gene Proteins 0.000 description 1
- 101150106114 oadG gene Proteins 0.000 description 1
- 230000004108 pentose phosphate pathway Effects 0.000 description 1
- 108010071189 phosphoenolpyruvate-glucose phosphotransferase Proteins 0.000 description 1
- 230000026731 phosphorylation Effects 0.000 description 1
- 238000006366 phosphorylation reaction Methods 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 108060006633 protein kinase Proteins 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 108020002667 ribulokinase Proteins 0.000 description 1
- 239000007320 rich medium Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 230000004102 tricarboxylic acid cycle Effects 0.000 description 1
- 108010062110 water dikinase pyruvate Proteins 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/44—Polycarboxylic acids
- C12P7/46—Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/02—Bacterial antigens
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/285—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Pasteurellaceae (F), e.g. Haemophilus influenza
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/001—Amines; Imines
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/14—Glutamic acid; Glutamine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/20—Aspartic acid; Asparagine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/44—Polycarboxylic acids
- C12P7/50—Polycarboxylic acids having keto groups, e.g. 2-ketoglutaric acid
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Epidemiology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Mycology (AREA)
- Communicable Diseases (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US70584105P | 2005-08-05 | 2005-08-05 | |
US60/705,841 | 2005-08-05 | ||
PCT/US2006/030425 WO2007019301A2 (fr) | 2005-08-05 | 2006-08-04 | Genes issus de d'actinobacillus succinogenes 130z (atcc 55618) pour la production d'agents chimiques issus de la voie c4 d'a. succinogenes |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2618694A1 true CA2618694A1 (fr) | 2007-02-15 |
Family
ID=37727918
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002618694A Abandoned CA2618694A1 (fr) | 2005-08-05 | 2006-08-04 | Genes issus de d'actinobacillus succinogenes 130z (atcc 55618) pour la production d'agents chimiques issus de la voie c4 d'a. succinogenes |
Country Status (7)
Country | Link |
---|---|
EP (1) | EP1922405A4 (fr) |
KR (1) | KR20080031504A (fr) |
CN (1) | CN101278041A (fr) |
BR (1) | BRPI0614716A2 (fr) |
CA (1) | CA2618694A1 (fr) |
MX (1) | MX2008001716A (fr) |
WO (1) | WO2007019301A2 (fr) |
Families Citing this family (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
BRPI0819717B1 (pt) | 2007-11-20 | 2022-05-17 | Dsm Ip Assets B.V. | Levedura recombinante e processo para a produção de ácido succínico |
JP5641938B2 (ja) | 2007-11-20 | 2014-12-17 | ディーエスエム アイピー アセッツ ビー.ブイ. | 真核細胞中におけるコハク酸の生成 |
EP2841572B1 (fr) | 2012-04-27 | 2019-06-19 | Duke University | Correction génétique de gènes ayant subi une mutation |
BE1021047B1 (fr) * | 2013-01-18 | 2015-02-25 | Man To Tree S.A. | Actinobacillus succinogenes genetiquement modifiee et son utilisation pour la production d'acide succinique |
CN103981203B (zh) | 2013-02-07 | 2018-01-12 | 中国科学院天津工业生物技术研究所 | 5‑氨基乙酰丙酸高产菌株及其制备方法和应用 |
CN105849555B (zh) * | 2013-12-18 | 2019-05-14 | 加利福尼亚太平洋生物科学股份有限公司 | 用于错误校正的序列读数迭代聚类 |
CN103695364B (zh) * | 2014-01-14 | 2018-02-02 | 中国科学院天津工业生物技术研究所 | 通过弱化5‑氨基乙酰丙酸脱水酶活性获得5‑氨基乙酰丙酸高产菌株及其应用 |
CN103710374B (zh) * | 2014-01-14 | 2018-02-06 | 中国科学院天津工业生物技术研究所 | 一种5‑氨基乙酰丙酸产生菌株及其制备方法与应用 |
BR112016018195A2 (pt) | 2014-02-07 | 2018-02-20 | Basf Se | micro-organismo modificado, método de produção de um composto orgânico e uso de um micro-organismo modificado |
CN104357497B (zh) * | 2014-10-22 | 2018-04-27 | 江南大学 | 一种提高产酸丙酸杆菌丙酸产量的方法 |
EP3362571A4 (fr) | 2015-10-13 | 2019-07-10 | Duke University | Ingénierie génomique avec systèmes crispr de type i dans des cellules eucaryotes |
US10428359B2 (en) * | 2016-10-03 | 2019-10-01 | Ajinomoto Co, Inc. | Method for producing L-amino acid |
US11279955B2 (en) | 2017-05-19 | 2022-03-22 | Basf Se | Process for producing an organic compound |
CN108517327B (zh) * | 2018-04-20 | 2020-10-30 | 中国科学院天津工业生物技术研究所 | 5-氨基乙酰丙酸高产菌株及其制备方法和应用 |
CN110904018B (zh) * | 2018-09-14 | 2022-09-09 | 中国科学院天津工业生物技术研究所 | 5-氨基乙酰丙酸生产菌株及其构建方法和应用 |
US20230383297A1 (en) * | 2020-10-09 | 2023-11-30 | Duke University | Novel targets for reactivation of prader-willi syndrome-associated genes |
WO2023095896A1 (fr) * | 2021-11-26 | 2023-06-01 | Dic株式会社 | Micro-organisme génétiquement modifié et procédé de production d'asparagine |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6696561B1 (en) * | 1909-07-09 | 2004-02-24 | Basf Aktiengesellschaft | Corynebacterium glutamicum genes encoding proteins involved in membrane synthesis and membrane transport |
US20030087381A1 (en) * | 1998-04-13 | 2003-05-08 | University Of Georgia Research Foundation, Inc. | Metabolically engineered organisms for enhanced production of oxaloacetate-derived biochemicals |
US6495023B1 (en) * | 1998-07-09 | 2002-12-17 | Michigan State University | Electrochemical methods for generation of a biological proton motive force and pyridine nucleotide cofactor regeneration |
US6599732B1 (en) * | 1999-06-29 | 2003-07-29 | Archer-Daniels-Midland Company | Regulation of carbon assimilation |
US20040152159A1 (en) * | 2002-11-06 | 2004-08-05 | Causey Thomas B. | Materials and methods for the efficient production of acetate and other products |
WO2004108894A2 (fr) * | 2003-05-30 | 2004-12-16 | Microbia, Inc. | Methodes et compositions destinees a la production d'acides amines |
US7393669B2 (en) * | 2003-07-07 | 2008-07-01 | Fluxome Sciences A/S | Metabolically engineered micro-organisms having improved galactose uptake |
US7163812B2 (en) * | 2003-08-06 | 2007-01-16 | Board Of Trustees Of Michigan State University | Actinobacillus succinogenes shuttle vector and methods of use |
-
2006
- 2006-08-04 EP EP06800746A patent/EP1922405A4/fr not_active Withdrawn
- 2006-08-04 MX MX2008001716A patent/MX2008001716A/es not_active Application Discontinuation
- 2006-08-04 WO PCT/US2006/030425 patent/WO2007019301A2/fr active Application Filing
- 2006-08-04 KR KR1020087005227A patent/KR20080031504A/ko not_active Application Discontinuation
- 2006-08-04 BR BRPI0614716-0A patent/BRPI0614716A2/pt not_active IP Right Cessation
- 2006-08-04 CA CA002618694A patent/CA2618694A1/fr not_active Abandoned
- 2006-08-04 CN CNA2006800367584A patent/CN101278041A/zh active Pending
Also Published As
Publication number | Publication date |
---|---|
BRPI0614716A2 (pt) | 2011-04-12 |
EP1922405A2 (fr) | 2008-05-21 |
MX2008001716A (es) | 2008-04-07 |
WO2007019301A3 (fr) | 2007-07-12 |
KR20080031504A (ko) | 2008-04-08 |
WO2007019301A2 (fr) | 2007-02-15 |
CN101278041A (zh) | 2008-10-01 |
EP1922405A4 (fr) | 2009-04-01 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CA2618694A1 (fr) | Genes issus de d'actinobacillus succinogenes 130z (atcc 55618) pour la production d'agents chimiques issus de la voie c4 d'a. succinogenes | |
Chen et al. | Metabolic engineering of Corynebacterium glutamicum for the production of 3-hydroxypropionic acid from glucose and xylose | |
Ikeda et al. | Identification and application of a different glucose uptake system that functions as an alternative to the phosphotransferase system in Corynebacterium glutamicum | |
Kawaguchi et al. | Engineering of a xylose metabolic pathway in Corynebacterium glutamicum | |
CA2659246C (fr) | Microorganisme produit par genie genetique produisant de l'acide homo-succinique et procede de preparation d'acide succinique au moyen dudit micro-organisme | |
US9228210B2 (en) | Process for producing an organic compound | |
US10717964B2 (en) | Synthetic methylotrophy to liquid fuels and chemicals | |
EP3280794B1 (fr) | Micro-organisme modifié pour optimiser la production d'efflux 2,4-dihydroxybutyrate avec des efflux de 2,4-dihydroxybutyrate améliorés | |
Ikeda | Lysine fermentation: history and genome breeding | |
JPWO2008133131A1 (ja) | 有機酸の製造方法 | |
JP5493854B2 (ja) | 有機酸の製造方法 | |
Gopinath et al. | Corynebacterium glutamicum as a potent biocatalyst for the bioconversion of pentose sugars to value-added products | |
CA2879680A1 (fr) | Xylose isomerases et leurs utilisations | |
US8962272B2 (en) | Engineered bacteria produce succinate from sucrose | |
US20110104771A1 (en) | Process for the production of a dicarboxylic acid | |
CN112481288B (zh) | 促进谷氨酸棒杆菌发酵生产目标产物的方法 | |
CN111705030B (zh) | 高产l-高丝氨酸的大肠杆菌基因工程菌、构建方法及菌株 | |
Yokota et al. | 10 Central Metabolism: Sugar Uptake and Conversion | |
Krahn et al. | Evolving a new efficient mode of fructose utilization for improved bioproduction in Corynebacterium glutamicum | |
CN111295446B (zh) | 引入高活性苹果酸脱氢酶用以生产琥珀酸的突变型微生物及使用其生产琥珀酸的方法 | |
KR101437041B1 (ko) | 숙신산 내성 효모균주를 이용한 숙신산의 제조방법 | |
WO2016030373A1 (fr) | Microorganisme modifié pour la production améliorée de produits chimiques fins sur du saccharose | |
KR101745333B1 (ko) | 수크로스에서 중등도 호열성 바실루스의 발효 | |
Fukui | Studies on Microbial Succinate Production | |
KR20230013492A (ko) | 이타콘산을 생산하는 신규 대사 경로 및 이를 이용한 이타콘산의 생산 방법 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
FZDE | Discontinued |