CA2603177C - Methods and means for removal of a selected dna sequence - Google Patents

Methods and means for removal of a selected dna sequence Download PDF

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Publication number
CA2603177C
CA2603177C CA2603177A CA2603177A CA2603177C CA 2603177 C CA2603177 C CA 2603177C CA 2603177 A CA2603177 A CA 2603177A CA 2603177 A CA2603177 A CA 2603177A CA 2603177 C CA2603177 C CA 2603177C
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Canada
Prior art keywords
dna
plant
flanking
sequence
enzyme
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2603177A
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English (en)
French (fr)
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CA2603177A1 (en
Inventor
Kathleen D'halluin
Rene Ruiter
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Bayer CropScience NV
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Bayer CropScience NV
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Publication of CA2603177A1 publication Critical patent/CA2603177A1/en
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Publication of CA2603177C publication Critical patent/CA2603177C/en
Expired - Fee Related legal-status Critical Current
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Sink And Installation For Waste Water (AREA)
  • Electrical Discharge Machining, Electrochemical Machining, And Combined Machining (AREA)
CA2603177A 2005-04-04 2006-03-31 Methods and means for removal of a selected dna sequence Expired - Fee Related CA2603177C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP05075781.4 2005-04-04
EP05075781 2005-04-04
US66924305P 2005-04-07 2005-04-07
US60/669,243 2005-04-07
PCT/EP2006/003086 WO2006105946A2 (en) 2005-04-04 2006-03-31 Methods and means for removal of a selected dna sequence

Publications (2)

Publication Number Publication Date
CA2603177A1 CA2603177A1 (en) 2006-10-12
CA2603177C true CA2603177C (en) 2016-01-26

Family

ID=39256902

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2603177A Expired - Fee Related CA2603177C (en) 2005-04-04 2006-03-31 Methods and means for removal of a selected dna sequence

Country Status (12)

Country Link
US (1) US8148607B2 (enExample)
EP (1) EP1869186B1 (enExample)
JP (1) JP5639336B2 (enExample)
CN (2) CN102286456B (enExample)
AT (1) ATE484594T1 (enExample)
AU (1) AU2006232828B2 (enExample)
CA (1) CA2603177C (enExample)
DE (1) DE602006017527D1 (enExample)
DK (1) DK1869186T3 (enExample)
ES (1) ES2354109T3 (enExample)
SI (1) SI1869186T1 (enExample)
WO (1) WO2006105946A2 (enExample)

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US20120196370A1 (en) 2010-12-03 2012-08-02 Fyodor Urnov Methods and compositions for targeted genomic deletion
AU2015264885B2 (en) * 2006-08-11 2017-08-17 Corteva Agriscience Llc Zinc finger nuclease-mediated homologous recombination
WO2008021207A2 (en) 2006-08-11 2008-02-21 Dow Agrosciences Llc Zinc finger nuclease-mediated homologous recombination
CA2664414C (en) * 2006-09-28 2016-07-12 Bayer Bioscience N.V. Methods and means for removal of a selected dna sequence
WO2008145731A1 (en) * 2007-05-31 2008-12-04 Basf Plant Science Gmbh Method of excising a nucleic acid sequence from a plant genome
CA2689345C (en) * 2007-06-05 2017-07-11 Bayer Bioscience N.V. Methods and means for exact replacement of target dna in eukaryotic organisms
WO2009006297A2 (en) 2007-06-29 2009-01-08 Pioneer Hi-Bred International, Inc. Methods for altering the genome of a monocot plant cell
WO2009114321A2 (en) 2008-03-11 2009-09-17 Precision Biosciencs, Inc. Rationally-designed meganucleases for maize genome engineering
JP2013517774A (ja) * 2010-01-22 2013-05-20 ダウ アグロサイエンシィズ エルエルシー 遺伝子改変生物における導入遺伝子の切除
SG185668A1 (en) 2010-06-09 2012-12-28 Bayer Cropscience Nv Methods and means to modify a plant genome at a nucleotide sequence commonly used in plant genome engineering
EP2580336B1 (en) 2010-06-09 2017-05-10 Bayer CropScience NV Methods and means to modify a plant genome at a nucleotide sequence commonly used in plant genome engineering
WO2012084742A1 (en) 2010-12-24 2012-06-28 Bayer Cropscience Nv Brassica plant comprising a mutant alcatraz allele
AU2012266597B2 (en) 2011-06-06 2016-09-22 Bayer Cropscience Nv Methods and means to modify a plant genome at a preselected site
MX346439B (es) 2011-08-22 2017-03-17 Bayer Cropscience Nv Métodos y medios para modificar un genoma vegetal.
EP2612918A1 (en) 2012-01-06 2013-07-10 BASF Plant Science Company GmbH In planta recombination
WO2013160230A1 (en) 2012-04-23 2013-10-31 Bayer Cropscience Nv Targeted genome engineering in plants
IN2014DN11215A (enExample) 2012-07-06 2015-10-02 Bayer Cropscience Nv
US9968041B2 (en) 2012-07-06 2018-05-15 Bayer Cropscience Nv Soybean ROD1 gene sequences and uses thereof
EP2877583A1 (en) 2012-07-06 2015-06-03 Bayer CropScience NV Brassica plants with modified seed oil composition
WO2014161821A1 (en) 2013-04-02 2014-10-09 Bayer Cropscience Nv Targeted genome engineering in eukaryotes
CN105073994A (zh) 2013-04-05 2015-11-18 拜尔作物科学公司 包含突变da1等位基因的芸苔属植物
UA122386C2 (uk) 2013-07-01 2020-11-10 Байєр Кропсаєнс Нв Спосіб прискорення цвітіння, розвитку насіння або уповільнення проростання насіння у однодольних рослин
WO2016050512A1 (en) 2014-10-03 2016-04-07 Bayer Cropscience Nv Methods and means for increasing stress tolerance and biomass in plants
WO2016050984A1 (en) 2014-10-03 2016-04-07 Vib Vzw Functional mutant alleles of ein5 for yield increase
WO2016174119A1 (en) 2015-04-28 2016-11-03 Bayer Cropscience Nv Brassica plants with modified seed oil composition
CN106554957B (zh) * 2015-09-30 2020-04-21 中国农业科学院深圳农业基因组研究所 测序文库及其制备和应用
EA201890964A1 (ru) 2015-10-16 2019-01-31 Байер Кропсайенс Нв Растения рода brassica с измененными характеристиками семенной продуктивности
CN109415737A (zh) * 2016-05-25 2019-03-01 嘉吉公司 用于在植物中产生突变的工程化核酸酶
US20190225974A1 (en) 2016-09-23 2019-07-25 BASF Agricultural Solutions Seed US LLC Targeted genome optimization in plants
EP3555285A4 (en) 2016-12-14 2020-07-08 Dow AgroSciences LLC RECONSTRUCTION OF SITE SPECIFIC NUCLEASE BINDING SITES
MX2020007466A (es) 2018-01-12 2020-11-12 Basf Se Gen subyacente al qtl de la cantidad de espiguillas por espiga en trigo en el cromosoma 7a.
CN112969792A (zh) 2018-09-07 2021-06-15 巴斯夫植物科学有限公司 用于在植物中产生高水平pufa的改进方法

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EP0317509A3 (de) 1987-11-16 1990-01-31 Ciba-Geigy Ag Gezielter Einbau von Genen ins pflanzliche Genom
US5436150A (en) 1992-04-03 1995-07-25 The Johns Hopkins University Functional domains in flavobacterium okeanokoities (foki) restriction endonuclease
US5792632A (en) 1992-05-05 1998-08-11 Institut Pasteur Nucleotide sequence encoding the enzyme I-SceI and the uses thereof
US5527695A (en) 1993-01-29 1996-06-18 Purdue Research Foundation Controlled modification of eukaryotic genomes
ATE513037T1 (de) 1993-02-12 2011-07-15 Univ Johns Hopkins Funktionelle domänen der restriktionsendonukleasen aus flavobakterium okeanokoites (foki)
US6013859A (en) * 1994-07-14 2000-01-11 Pioneer Hi-Bred International, Inc. Molecular methods of hybrid seed production
EP0790311A1 (en) * 1996-02-16 1997-08-20 Centrum Voor Plantenveredelings- En Reproduktieonderzoek Microspore-specific promoter from tobacco
FR2768745B1 (fr) 1997-09-23 2001-10-05 Agronomique Inst Nat Rech Promoteur specifique des microspores et procede d'obtention de plantes hybrides
US6632980B1 (en) 1997-10-24 2003-10-14 E. I. Du Pont De Nemours And Company Binary viral expression system in plants
CN1249133A (zh) * 1998-12-25 2000-04-05 北京大学 获得雄性不育植物的分子方法
WO2000046386A2 (en) 1999-02-03 2000-08-10 The Children's Medical Center Corporation Gene repair involving the induction of double-stranded dna cleavage at a chromosomal target site
US6750379B2 (en) 2000-03-09 2004-06-15 Dekalb Genetics Corporation Homologous recombination-mediated transgene alterations in plants
DE10131786A1 (de) * 2001-07-04 2003-01-16 Sungene Gmbh & Co Kgaa Rekombinationssysteme und Verfahren zum Entfernen von Nukleinsäuresequenzen aus dem Genom eukaryotischer Organismen
US20030232410A1 (en) 2002-03-21 2003-12-18 Monika Liljedahl Methods and compositions for using zinc finger endonucleases to enhance homologous recombination
EP1590468A2 (en) 2003-01-28 2005-11-02 Cellectis Use of meganucleases for inducing homologous recombination ex vivo and in toto in vertebrate somatic tissues and application thereof
AU2004291692B2 (en) * 2003-11-18 2010-06-24 BASF Agricultural Solutions Seed US LLC Improved targeted DNA insertion in plants
CA2578140A1 (en) * 2004-09-23 2006-03-30 Basf Plant Science Gmbh Recombination cassettes and methods for sequence excision in plants

Also Published As

Publication number Publication date
AU2006232828A1 (en) 2006-10-12
DK1869186T3 (da) 2011-01-24
ATE484594T1 (de) 2010-10-15
EP1869186A2 (en) 2007-12-26
CN101155921B (zh) 2013-02-06
AU2006232828B2 (en) 2011-01-27
ES2354109T3 (es) 2011-03-10
WO2006105946A2 (en) 2006-10-12
WO2006105946A3 (en) 2007-04-05
US20090089890A1 (en) 2009-04-02
EP1869186B1 (en) 2010-10-13
DE602006017527D1 (de) 2010-11-25
JP2008534023A (ja) 2008-08-28
CA2603177A1 (en) 2006-10-12
CN101155921A (zh) 2008-04-02
JP5639336B2 (ja) 2014-12-10
CN102286456B (zh) 2014-04-30
US8148607B2 (en) 2012-04-03
CN102286456A (zh) 2011-12-21
SI1869186T1 (sl) 2011-02-28

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