CA2597673C - Methods and compositions for detecting a drug resistant egfr mutant - Google Patents
Methods and compositions for detecting a drug resistant egfr mutant Download PDFInfo
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- CA2597673C CA2597673C CA2597673A CA2597673A CA2597673C CA 2597673 C CA2597673 C CA 2597673C CA 2597673 A CA2597673 A CA 2597673A CA 2597673 A CA2597673 A CA 2597673A CA 2597673 C CA2597673 C CA 2597673C
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- egfr
- mutation
- mutant
- sequence
- gefitinib
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
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| US12366585B2 (en) | 2006-05-18 | 2025-07-22 | Caris Mpi, Inc. | Molecular profiling of tumors |
| AU2009246398A1 (en) | 2008-05-14 | 2009-11-19 | Bristol-Myers Squibb Company | Predictors of patient response to treatment with EGF receptor inhibitors |
| US11351168B1 (en) | 2008-06-27 | 2022-06-07 | Celgene Car Llc | 2,4-disubstituted pyrimidines useful as kinase inhibitors |
| EP2361248B1 (en) | 2008-06-27 | 2018-09-19 | Celgene CAR LLC | Heteroaryl compounds and uses thereof |
| US8338439B2 (en) | 2008-06-27 | 2012-12-25 | Celgene Avilomics Research, Inc. | 2,4-disubstituted pyrimidines useful as kinase inhibitors |
| US9089844B2 (en) | 2010-11-01 | 2015-07-28 | Bio-Rad Laboratories, Inc. | System for forming emulsions |
| US8633015B2 (en) | 2008-09-23 | 2014-01-21 | Bio-Rad Laboratories, Inc. | Flow-based thermocycling system with thermoelectric cooler |
| US9156010B2 (en) | 2008-09-23 | 2015-10-13 | Bio-Rad Laboratories, Inc. | Droplet-based assay system |
| US8709762B2 (en) | 2010-03-02 | 2014-04-29 | Bio-Rad Laboratories, Inc. | System for hot-start amplification via a multiple emulsion |
| US9132394B2 (en) | 2008-09-23 | 2015-09-15 | Bio-Rad Laboratories, Inc. | System for detection of spaced droplets |
| US9598725B2 (en) | 2010-03-02 | 2017-03-21 | Bio-Rad Laboratories, Inc. | Emulsion chemistry for encapsulated droplets |
| US12162008B2 (en) | 2008-09-23 | 2024-12-10 | Bio-Rad Laboratories, Inc. | Partition-based method of analysis |
| US12090480B2 (en) | 2008-09-23 | 2024-09-17 | Bio-Rad Laboratories, Inc. | Partition-based method of analysis |
| US9399215B2 (en) | 2012-04-13 | 2016-07-26 | Bio-Rad Laboratories, Inc. | Sample holder with a well having a wicking promoter |
| US11130128B2 (en) | 2008-09-23 | 2021-09-28 | Bio-Rad Laboratories, Inc. | Detection method for a target nucleic acid |
| US9492797B2 (en) | 2008-09-23 | 2016-11-15 | Bio-Rad Laboratories, Inc. | System for detection of spaced droplets |
| US10512910B2 (en) | 2008-09-23 | 2019-12-24 | Bio-Rad Laboratories, Inc. | Droplet-based analysis method |
| CN102405402A (zh) | 2008-09-23 | 2012-04-04 | 阔达生命有限公司 | 基于液滴的测定系统 |
| US9417190B2 (en) | 2008-09-23 | 2016-08-16 | Bio-Rad Laboratories, Inc. | Calibrations and controls for droplet-based assays |
| US9764322B2 (en) | 2008-09-23 | 2017-09-19 | Bio-Rad Laboratories, Inc. | System for generating droplets with pressure monitoring |
| WO2011120024A1 (en) | 2010-03-25 | 2011-09-29 | Quantalife, Inc. | Droplet generation for droplet-based assays |
| WO2011120006A1 (en) | 2010-03-25 | 2011-09-29 | Auantalife, Inc. A Delaware Corporation | Detection system for droplet-based assays |
| US8951939B2 (en) | 2011-07-12 | 2015-02-10 | Bio-Rad Laboratories, Inc. | Digital assays with multiplexed detection of two or more targets in the same optical channel |
| CN101463390B (zh) * | 2008-12-31 | 2011-09-14 | 广州益善生物技术有限公司 | Egfr基因外显子20突变的检测探针、液相芯片及其检测方法 |
| EP3722810A3 (en) * | 2009-02-11 | 2021-01-13 | Caris MPI, Inc. | Molecular profiling of tumors |
| CN101608240B (zh) * | 2009-04-13 | 2011-06-29 | 郑立谋 | 用于检测人类egfr基因突变的引物、探针及其使用方法 |
| JP5918693B2 (ja) | 2009-05-05 | 2016-05-18 | ダナ ファーバー キャンサー インスティテュート インコーポレイテッド | Ｅｇｆｒ阻害剤及び疾患の治療方法 |
| CA3021714C (en) | 2009-09-02 | 2021-03-09 | Bio-Rad Laboratories, Inc. | System for mixing fluids by coalescence of multiple emulsions |
| CN102021232A (zh) * | 2009-09-22 | 2011-04-20 | 北京雅康博生物科技有限公司 | 用于定量检测egfr突变的试剂盒 |
| KR101472940B1 (ko) | 2009-10-30 | 2014-12-24 | 아크레이 가부시키가이샤 | Ｅｇｆｒ 유전자 다형 검출용 프로브 및 그 용도 |
| WO2011079231A1 (en) | 2009-12-23 | 2011-06-30 | Gatekeeper Pharmaceutical, Inc. | Compounds that modulate egfr activity and methods for treating or preventing conditions therewith |
| EP2542692B1 (en) | 2010-03-04 | 2016-08-24 | Carpén, Olli | Method for selecting patients for treatment with an egfr inhibitor |
| EP2556170A4 (en) | 2010-03-25 | 2014-01-01 | Quantalife Inc | TRAPPING TRANSPORT AND DETECTION SYSTEM |
| CN102234683B (zh) * | 2010-04-23 | 2013-07-17 | 广州益善生物技术有限公司 | 一种egfr基因突变检测液相芯片 |
| NZ607845A (en) | 2010-08-10 | 2015-03-27 | Celgene Avilomics Res Inc | Besylate salt of a btk inhibitor |
| US9315848B2 (en) | 2010-08-18 | 2016-04-19 | Technion Research And Development Foundation Ltd. | Volatile organic compounds for detecting cell dysplasia and genetic alterations associated with lung cancer |
| JP5860667B2 (ja) | 2010-10-29 | 2016-02-16 | アークレイ株式会社 | Ｅｇｆｒエクソン２１ｌ８５８ｒ遺伝子多型検出用プライマーセット及びその用途 |
| EP2635285B1 (en) | 2010-11-01 | 2017-05-03 | Celgene Avilomics Research, Inc. | Heteroaryl compounds and uses thereof |
| WO2012061299A1 (en) | 2010-11-01 | 2012-05-10 | Avila Therapeutics, Inc. | Heterocyclic compounds and uses thereof |
| US8796255B2 (en) | 2010-11-10 | 2014-08-05 | Celgene Avilomics Research, Inc | Mutant-selective EGFR inhibitors and uses thereof |
| EP2468883A1 (en) * | 2010-12-22 | 2012-06-27 | Pangaea Biotech S.L. | Molecular biomarkers for predicting response to tyrosine kinase inhibitors in lung cancer |
| US12097495B2 (en) | 2011-02-18 | 2024-09-24 | Bio-Rad Laboratories, Inc. | Methods and compositions for detecting genetic material |
| CN103534360A (zh) | 2011-03-18 | 2014-01-22 | 伯乐生命医学产品有限公司 | 借助对信号的组合使用进行的多重数字分析 |
| AU2012249759A1 (en) | 2011-04-25 | 2013-11-07 | Bio-Rad Laboratories, Inc. | Methods and compositions for nucleic acid analysis |
| EP2737089B1 (en) | 2011-07-29 | 2017-09-06 | Bio-rad Laboratories, Inc. | Library characterization by digital assay |
| EP2770830A4 (en) | 2011-10-28 | 2015-05-27 | Celgene Avilomics Res Inc | METHODS OF TREATING A DISEASE OR DISEASE ASSOCIATED WITH TYROSINE KINASE BTK (BRUTON'S TYROSINE KINASE) |
| CN103184274A (zh) * | 2011-12-27 | 2013-07-03 | 上海复星医学科技发展有限公司 | 一种ldr技术的egfr突变检测试剂盒 |
| MX356753B (es) | 2012-03-15 | 2018-06-12 | Celgene Avilomics Res Inc | Formas solidas de un inhibidor de cinasa del receptor del factor de crecimiento epidermico. |
| PT2825042T (pt) | 2012-03-15 | 2018-11-16 | Celgene Car Llc | Sais de um inibidor de cinases do recetor do fator de crescimento epidérmico |
| US10260089B2 (en) | 2012-10-29 | 2019-04-16 | The Research Foundation Of The State University Of New York | Compositions and methods for recognition of RNA using triple helical peptide nucleic acids |
| US9126950B2 (en) | 2012-12-21 | 2015-09-08 | Celgene Avilomics Research, Inc. | Heteroaryl compounds and uses thereof |
| WO2014124230A2 (en) | 2013-02-08 | 2014-08-14 | Celgene Avilomics Research, Inc. | Erk inhibitors and uses thereof |
| US20140287417A1 (en) | 2013-03-08 | 2014-09-25 | Roche Molecular Systems, Inc. | EGFR Blood Monitoring |
| EP2964781B1 (en) | 2013-03-08 | 2018-01-10 | Roche Diagnostics GmbH | Egfr mutation blood testing |
| WO2014147631A1 (en) | 2013-03-22 | 2014-09-25 | Natco Pharma Limited | Formulation comprising gefitinib as oral suspension |
| US9492471B2 (en) | 2013-08-27 | 2016-11-15 | Celgene Avilomics Research, Inc. | Methods of treating a disease or disorder associated with Bruton'S Tyrosine Kinase |
| US9415049B2 (en) | 2013-12-20 | 2016-08-16 | Celgene Avilomics Research, Inc. | Heteroaryl compounds and uses thereof |
| GB201402996D0 (en) * | 2014-02-20 | 2014-04-09 | Vela Operations Pte Ltd | Variant analysis in high-throughput sequencing applications |
| WO2015187855A1 (en) * | 2014-06-03 | 2015-12-10 | The Regents Of The University Of California | Non-invasive gene mutation detection in lung cancer patients |
| ES2741785T3 (es) | 2014-08-13 | 2020-02-12 | Celgene Car Llc | Formas y composiciones de un inhibidor de ERK |
| CN106498028B (zh) * | 2016-05-26 | 2020-06-30 | 格诺思博生物科技南通有限公司 | Egfr的t790m突变的诊断方法及试剂盒 |
| SG11201810694WA (en) * | 2016-06-03 | 2018-12-28 | Singapore Health Serv Pte Ltd | Use of biomarkers in determining susceptibility to disease treatment |
| CN106498029B (zh) * | 2016-06-20 | 2020-05-19 | 格诺思博生物科技南通有限公司 | 提高egfr的t790m突变的诊断效率的方法 |
| JP2018088883A (ja) * | 2016-12-06 | 2018-06-14 | 東洋紡株式会社 | 上皮成長因子受容体遺伝子変異の検出方法 |
| CN106811522A (zh) * | 2016-12-26 | 2017-06-09 | 北京奥维森基因科技有限公司 | 用于检测以egfp为靶点的药物相关基因耐药位点的pcr引物组及其应用和检测试剂盒 |
| US9980967B1 (en) | 2017-03-16 | 2018-05-29 | National Chiao Tung University | Method for overcoming drug resistance of EGFR mutation and cancerous stemness of human non-small cell lung carcinoma |
| CN106906295B (zh) * | 2017-03-31 | 2020-12-08 | 天津诺禾致源生物信息科技有限公司 | 数字pcr检测基因点突变方法及装置 |
| CN108546748A (zh) * | 2017-04-05 | 2018-09-18 | 杭州丹威生物科技有限公司 | 一种检测核酸的方法和试剂盒 |
| CN109722478A (zh) * | 2017-10-31 | 2019-05-07 | 嘉兴雅康博医学检验所有限公司 | 用于检测egfr基因2312-2313位突变的引物、探针及试剂盒 |
| WO2019176939A1 (ja) | 2018-03-15 | 2019-09-19 | 栄研化学株式会社 | 一塩基多型検出用オリゴヌクレオチドプローブ、及びシス型－トランス型判別方法 |
| US11459391B2 (en) | 2019-02-26 | 2022-10-04 | Janssen Biotech, Inc. | Combination therapies and patient stratification with bispecific anti-EGFR/c-Met antibodies |
| CN111748026A (zh) * | 2019-03-29 | 2020-10-09 | 天津亨佳生物科技发展有限公司 | 一种针对egfr l858r基因突变的特异性t细胞受体及其应用 |
| JOP20210304A1 (ar) | 2019-05-14 | 2023-01-30 | Janssen Biotech Inc | علاجات مركبة باستخدام الأجسام المضادة ثنائية النوعية المضادة لمستقبل عامل نمو البشرة (EGFR)/ مستقبل عامل نمو خلايا الكبد (c-Met) ومثبطات كيناز التيروسين الخاصة بمستقبل عامل نمو البشرة (EGFR) من الجيل الثالث |
| JOP20220184A1 (ar) | 2020-02-12 | 2023-01-30 | Janssen Biotech Inc | علاج مصابي السرطان ممن لديهم طفرات تخطي c-Met إكسون14 |
| KR20230039608A (ko) * | 2020-05-07 | 2023-03-21 | 더 리전츠 오브 더 유니버시티 오브 캘리포니아 | 혈장 및 타액의 액체 생검 플랫폼 |
Family Cites Families (53)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3687808A (en) | 1969-08-14 | 1972-08-29 | Univ Leland Stanford Junior | Synthetic polynucleotides |
| US4182124A (en) | 1977-01-28 | 1980-01-08 | Kraus Edmund J | Gravity augmented air compression turbine power plant |
| US4196265A (en) | 1977-06-15 | 1980-04-01 | The Wistar Institute | Method of producing antibodies |
| CA1142466A (en) | 1979-01-09 | 1983-03-08 | Cesar Milstein | Cell lines |
| US5132418A (en) | 1980-02-29 | 1992-07-21 | University Patents, Inc. | Process for preparing polynucleotides |
| US4458066A (en) | 1980-02-29 | 1984-07-03 | University Patents, Inc. | Process for preparing polynucleotides |
| US4500707A (en) | 1980-02-29 | 1985-02-19 | University Patents, Inc. | Nucleosides useful in the preparation of polynucleotides |
| US4668777A (en) | 1981-03-27 | 1987-05-26 | University Patents, Inc. | Phosphoramidite nucleoside compounds |
| US4973679A (en) | 1981-03-27 | 1990-11-27 | University Patents, Inc. | Process for oligonucleo tide synthesis using phosphormidite intermediates |
| US4415732A (en) | 1981-03-27 | 1983-11-15 | University Patents, Inc. | Phosphoramidite compounds and processes |
| US5030565A (en) | 1983-08-17 | 1991-07-09 | Scripps Clinic And Research Foundation | Polypeptide-induced monoclonal receptors to protein ligands |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US5153319A (en) | 1986-03-31 | 1992-10-06 | University Patents, Inc. | Process for preparing polynucleotides |
| US5561058A (en) | 1986-08-22 | 1996-10-01 | Hoffmann-La Roche Inc. | Methods for coupled high temperatures reverse transcription and polymerase chain reactions |
| CA1339731C (en) | 1988-10-12 | 1998-03-17 | Charles T. Caskey | Multiplex genomic dna amplification for deletion detection |
| US5223168A (en) | 1989-12-12 | 1993-06-29 | Gary Holt | Surface cleaner and treatment |
| US5506351A (en) | 1992-07-23 | 1996-04-09 | Isis Pharmaceuticals | Process for the preparation of 2'-O-alkyl guanosine and related compounds |
| US5212295A (en) | 1990-01-11 | 1993-05-18 | Isis Pharmaceuticals | Monomers for preparation of oligonucleotides having chiral phosphorus linkages |
| US5587361A (en) | 1991-10-15 | 1996-12-24 | Isis Pharmaceuticals, Inc. | Oligonucleotides having phosphorothioate linkages of high chiral purity |
| US5587470A (en) | 1990-01-11 | 1996-12-24 | Isis Pharmaceuticals, Inc. | 3-deazapurines |
| US5578718A (en) | 1990-01-11 | 1996-11-26 | Isis Pharmaceuticals, Inc. | Thiol-derivatized nucleosides |
| US5459255A (en) | 1990-01-11 | 1995-10-17 | Isis Pharmaceuticals, Inc. | N-2 substituted purines |
| US5457191A (en) | 1990-01-11 | 1995-10-10 | Isis Pharmaceuticals, Inc. | 3-deazapurines |
| DE69131255T2 (de) | 1990-01-26 | 1999-12-16 | Washington Research Foundation, Seattle | Immunreaktivität gegenüber exprimierten aktivierten Oncogenen zur Diagnose und Behandlung von bösartigen Geschwülsten |
| US5378825A (en) | 1990-07-27 | 1995-01-03 | Isis Pharmaceuticals, Inc. | Backbone modified oligonucleotide analogs |
| US5218105A (en) | 1990-07-27 | 1993-06-08 | Isis Pharmaceuticals | Polyamine conjugated oligonucleotides |
| US5610289A (en) | 1990-07-27 | 1997-03-11 | Isis Pharmaceuticals, Inc. | Backbone modified oligonucleotide analogues |
| US5138045A (en) | 1990-07-27 | 1992-08-11 | Isis Pharmaceuticals | Polyamine conjugated oligonucleotides |
| US5386023A (en) | 1990-07-27 | 1995-01-31 | Isis Pharmaceuticals | Backbone modified oligonucleotide analogs and preparation thereof through reductive coupling |
| US5541307A (en) | 1990-07-27 | 1996-07-30 | Isis Pharmaceuticals, Inc. | Backbone modified oligonucleotide analogs and solid phase synthesis thereof |
| US5602240A (en) | 1990-07-27 | 1997-02-11 | Ciba Geigy Ag. | Backbone modified oligonucleotide analogs |
| US5608046A (en) | 1990-07-27 | 1997-03-04 | Isis Pharmaceuticals, Inc. | Conjugated 4'-desmethyl nucleoside analog compounds |
| US6262241B1 (en) | 1990-08-13 | 2001-07-17 | Isis Pharmaceuticals, Inc. | Compound for detecting and modulating RNA activity and gene expression |
| US5852184A (en) | 1990-11-28 | 1998-12-22 | Ludwig Institute For Cancer Research | Protein tyrosine kinase |
| US5610276A (en) | 1991-05-17 | 1997-03-11 | Chiron Corporation | Polypeptides and antibodies derived from GAP associated protein, p62 |
| US5539082A (en) | 1993-04-26 | 1996-07-23 | Nielsen; Peter E. | Peptide nucleic acids |
| US5599797A (en) | 1991-10-15 | 1997-02-04 | Isis Pharmaceuticals, Inc. | Oligonucleotides having phosphorothioate linkages of high chiral purity |
| US5633134A (en) | 1992-10-06 | 1997-05-27 | Ig Laboratories, Inc. | Method for simultaneously detecting multiple mutations in a DNA sample |
| US5405941A (en) | 1993-04-15 | 1995-04-11 | National Jewish Center For Immunology And Respiratory Medicine | MEKK protein, capable of phosphorylating MEK |
| US5571902A (en) | 1993-07-29 | 1996-11-05 | Isis Pharmaceuticals, Inc. | Synthesis of oligonucleotides |
| US5554746A (en) | 1994-05-16 | 1996-09-10 | Isis Pharmaceuticals, Inc. | Lactam nucleic acids |
| US5589330A (en) | 1994-07-28 | 1996-12-31 | Genzyme Corporation | High-throughput screening method for sequence or genetic alterations in nucleic acids using elution and sequencing of complementary oligonucleotides |
| US5939269A (en) | 1994-12-28 | 1999-08-17 | The Regents Of The University Of California | Antagonists to insulin receptor tyrosine kinase inhibitor |
| US6066625A (en) | 1998-02-03 | 2000-05-23 | Methylgene, Inc. | Optimized antisense oligonucleotides complementary to DNA methyltransferase sequences |
| US6503754B1 (en) | 2000-09-07 | 2003-01-07 | Isis Pharmaceuticals, Inc. | Antisense modulation of BH3 interacting domain death agonist expression |
| EP2186901B1 (en) | 2001-06-14 | 2015-06-03 | The Regents of The University of California | Mutations in the BCR-Abl-tyrosine kinase associated with resistance to STI-571 |
| EP2439285B1 (en) | 2004-03-31 | 2019-05-08 | The General Hospital Corporation | Method to determine responsiveness of cancer to epidermal growth factor receptor targeting treatments |
| EP2592155B2 (en) | 2004-06-04 | 2019-09-11 | Genentech, Inc. | EGFR mutations |
| RU2405566C9 (ru) | 2005-02-03 | 2012-04-27 | Дзе Дженерал Хоспитал Корпорейшн | Способ лечения рака, устойчивого к гефитинибу |
| WO2006086777A2 (en) * | 2005-02-11 | 2006-08-17 | Memorial Sloan Kettering Cancer Center | Methods and compositions for detecting a drug resistant egfr mutant |
| KR20070106029A (ko) | 2005-02-24 | 2007-10-31 | 암젠 인코포레이티드 | 상피세포 성장 인자 수용체 돌연변이 |
| EP1881079A1 (en) | 2006-07-20 | 2008-01-23 | Pangaea Biotech, S.A. | Method for the detection of EGFR mutations in blood samples |
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| CA2597673A1 (en) | 2006-08-17 |
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| CN101193905B (zh) | 2014-06-25 |
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| WO2006086777A2 (en) | 2006-08-17 |
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| US8501413B2 (en) | 2013-08-06 |
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| EP1851339A2 (en) | 2007-11-07 |
| EP1851339A4 (en) | 2009-05-27 |
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