CA2540822C - Detection of no by using guanylyl cyclase and cgmp production as a readout system - Google Patents
Detection of no by using guanylyl cyclase and cgmp production as a readout system Download PDFInfo
- Publication number
- CA2540822C CA2540822C CA2540822A CA2540822A CA2540822C CA 2540822 C CA2540822 C CA 2540822C CA 2540822 A CA2540822 A CA 2540822A CA 2540822 A CA2540822 A CA 2540822A CA 2540822 C CA2540822 C CA 2540822C
- Authority
- CA
- Canada
- Prior art keywords
- cgmp
- nitric oxide
- nos
- oxide synthase
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 108010078321 Guanylate Cyclase Proteins 0.000 title claims abstract description 35
- 102000014469 Guanylate cyclase Human genes 0.000 title claims abstract description 35
- 238000001514 detection method Methods 0.000 title claims description 32
- 238000004519 manufacturing process Methods 0.000 title description 8
- MWUXSHHQAYIFBG-UHFFFAOYSA-N Nitric oxide Chemical compound O=[N] MWUXSHHQAYIFBG-UHFFFAOYSA-N 0.000 claims abstract description 133
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- 108010021487 Nitric Oxide Synthase Proteins 0.000 claims abstract description 98
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- ZOOGRGPOEVQQDX-KHLHZJAASA-N cyclic guanosine monophosphate Chemical compound C([C@H]1O2)O[P@](O)(=O)O[C@@H]1[C@H](O)[C@H]2N1C(N=C(NC2=O)N)=C2N=C1 ZOOGRGPOEVQQDX-KHLHZJAASA-N 0.000 claims 19
- 125000000637 arginyl group Chemical group N[C@@H](CCCNC(N)=N)C(=O)* 0.000 claims 1
- ZOOGRGPOEVQQDX-UUOKFMHZSA-N 3',5'-cyclic GMP Chemical compound C([C@H]1O2)OP(O)(=O)O[C@H]1[C@@H](O)[C@@H]2N1C(N=C(NC2=O)N)=C2N=C1 ZOOGRGPOEVQQDX-UUOKFMHZSA-N 0.000 abstract description 5
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- 125000001417 5'-guanylyl group Chemical group C=12N=C(N([H])[H])N([H])C(=O)C=1N=C([H])N2[C@@]1([H])[C@@](O[H])([H])[C@@](O[H])([H])[C@](C(OP(=O)(O[H])[*])([H])[H])([H])O1 0.000 description 3
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- XEYBHCRIKKKOSS-UHFFFAOYSA-N disodium;azanylidyneoxidanium;iron(2+);pentacyanide Chemical compound [Na+].[Na+].[Fe+2].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].[O+]#N XEYBHCRIKKKOSS-UHFFFAOYSA-N 0.000 description 1
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- 238000007254 oxidation reaction Methods 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
- A61K31/19—Carboxylic acids, e.g. valproic acid
- A61K31/195—Carboxylic acids, e.g. valproic acid having an amino group
- A61K31/197—Carboxylic acids, e.g. valproic acid having an amino group the amino and the carboxyl groups being attached to the same acyclic carbon chain, e.g. gamma-aminobutyric acid [GABA], beta-alanine, epsilon-aminocaproic acid or pantothenic acid
- A61K31/198—Alpha-amino acids, e.g. alanine or edetic acid [EDTA]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Epidemiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP03027159 | 2003-11-26 | ||
| EP03027159.7 | 2003-11-26 | ||
| PCT/EP2004/013707 WO2005052185A1 (en) | 2003-11-26 | 2004-11-25 | Detection of no by using guanylyl cyclase and cgmp production as a readout system |
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| CA2540822A1 CA2540822A1 (en) | 2005-06-09 |
| CA2540822C true CA2540822C (en) | 2010-09-21 |
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| CA2540822A Expired - Lifetime CA2540822C (en) | 2003-11-26 | 2004-11-25 | Detection of no by using guanylyl cyclase and cgmp production as a readout system |
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| EP (1) | EP1689880B1 (cg-RX-API-DMAC7.html) |
| JP (1) | JP5671194B2 (cg-RX-API-DMAC7.html) |
| AT (1) | ATE429508T1 (cg-RX-API-DMAC7.html) |
| CA (1) | CA2540822C (cg-RX-API-DMAC7.html) |
| DE (1) | DE602004020795D1 (cg-RX-API-DMAC7.html) |
| DK (1) | DK1689880T3 (cg-RX-API-DMAC7.html) |
| ES (1) | ES2324477T3 (cg-RX-API-DMAC7.html) |
| WO (1) | WO2005052185A1 (cg-RX-API-DMAC7.html) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102006038942A1 (de) * | 2006-08-18 | 2008-02-21 | Bayer Healthcare Ag | Verfahren zur Identifizierung von Stickstoffmonoxid Modulatoren |
| JP5350607B2 (ja) * | 2007-06-14 | 2013-11-27 | キッコーマン株式会社 | グアニル酸シクラーゼ活性測定法 |
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| DE10043845A1 (de) * | 2000-09-06 | 2002-03-14 | Gruenenthal Gmbh | Verfahren zur Messung der Aktivität der NO-Synthase |
-
2004
- 2004-11-25 WO PCT/EP2004/013707 patent/WO2005052185A1/en not_active Ceased
- 2004-11-25 ES ES04819231T patent/ES2324477T3/es not_active Expired - Lifetime
- 2004-11-25 DE DE602004020795T patent/DE602004020795D1/de not_active Expired - Lifetime
- 2004-11-25 JP JP2006540410A patent/JP5671194B2/ja not_active Expired - Lifetime
- 2004-11-25 CA CA2540822A patent/CA2540822C/en not_active Expired - Lifetime
- 2004-11-25 DK DK04819231T patent/DK1689880T3/da active
- 2004-11-25 EP EP04819231A patent/EP1689880B1/en not_active Expired - Lifetime
- 2004-11-25 AT AT04819231T patent/ATE429508T1/de active
Also Published As
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|---|---|
| JP5671194B2 (ja) | 2015-02-18 |
| WO2005052185A1 (en) | 2005-06-09 |
| JP2007512011A (ja) | 2007-05-17 |
| EP1689880B1 (en) | 2009-04-22 |
| ATE429508T1 (de) | 2009-05-15 |
| DE602004020795D1 (de) | 2009-06-04 |
| CA2540822A1 (en) | 2005-06-09 |
| ES2324477T3 (es) | 2009-08-07 |
| DK1689880T3 (da) | 2009-07-27 |
| EP1689880A1 (en) | 2006-08-16 |
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