CA2506999C - Systemes d'expression pouvant etre induits par le l-rhamnose - Google Patents

Systemes d'expression pouvant etre induits par le l-rhamnose Download PDF

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Publication number
CA2506999C
CA2506999C CA2506999A CA2506999A CA2506999C CA 2506999 C CA2506999 C CA 2506999C CA 2506999 A CA2506999 A CA 2506999A CA 2506999 A CA2506999 A CA 2506999A CA 2506999 C CA2506999 C CA 2506999C
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CA
Canada
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leu
ala
gly
ser
val
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Expired - Fee Related
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CA2506999A
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CA2506999A1 (fr
Inventor
Maria Kesseler
Thomas Zelinski
Bernhard Hauer
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BASF SE
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BASF SE
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Publication of CA2506999A1 publication Critical patent/CA2506999A1/fr
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Detergent Compositions (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Curing Cements, Concrete, And Artificial Stone (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'invention concerne un procédé permettant d'exprimer des séquences d'acides nucléiques dans des cellules hôtes procaryotes. Au moins une structure ADN réplicable de manière épisomique dans lesdites cellules hôtes, comprenant une séquence d'acides nucléiques à exprimer, est introduite dans lesdites cellules hôtes par contrôle transcriptionnel d'un promoteur pouvant être induit par un L-rhamnose, ledit promoteur étant hétérologue par rapport à la séquence d'acides nucléiques. L'expression de ladite séquence d'acides nucléiques est induite par addition d'un L-rahmnose. Le procédé de l'invention est caractérisé en ce que la cellule hôte procaryote est au moins déficiente par rapport à une L-rahmnose isomérase.
CA2506999A 2002-12-02 2003-11-27 Systemes d'expression pouvant etre induits par le l-rhamnose Expired - Fee Related CA2506999C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10256381.0 2002-12-02
DE10256381 2002-12-02
PCT/EP2003/013367 WO2004050877A1 (fr) 2002-12-02 2003-11-27 Systemes d'expression pouvant etre induits par le l-rhamnose

Publications (2)

Publication Number Publication Date
CA2506999A1 CA2506999A1 (fr) 2004-06-17
CA2506999C true CA2506999C (fr) 2014-01-07

Family

ID=32403685

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2506999A Expired - Fee Related CA2506999C (fr) 2002-12-02 2003-11-27 Systemes d'expression pouvant etre induits par le l-rhamnose

Country Status (13)

Country Link
US (1) US8895310B2 (fr)
EP (1) EP1570060B1 (fr)
JP (1) JP4619126B2 (fr)
CN (1) CN100445391C (fr)
AT (1) ATE368746T1 (fr)
AU (1) AU2003288189B8 (fr)
CA (1) CA2506999C (fr)
CY (1) CY1107772T1 (fr)
DE (1) DE50307835D1 (fr)
DK (1) DK1570060T3 (fr)
ES (1) ES2288630T3 (fr)
PT (1) PT1570060E (fr)
WO (1) WO2004050877A1 (fr)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030194798A1 (en) * 2001-05-24 2003-10-16 Surber Mark W. Minicell compositions and methods
CA2541177A1 (fr) * 2003-10-03 2005-09-22 Promega Corporation Vecteurs pour le clonage directionnel
US8293503B2 (en) 2003-10-03 2012-10-23 Promega Corporation Vectors for directional cloning
EP2123761A3 (fr) 2004-12-07 2009-12-16 Lonza Ag Système d'expression utilisant un promoteur inductible par le rhamnose
AU2013250804A1 (en) * 2012-04-17 2016-08-11 Agtive Bio-Sciences Private Limited Method of production of monosaccharides
CN104603274B (zh) * 2012-08-05 2020-08-18 Absci有限责任公司 可诱导共表达系统
CA2947941C (fr) * 2014-03-05 2021-02-23 National University Corporation Kobe University Procede de modification de sequence genomique permettant la conversion de facon specifique de bases d'acide nucleique de sequences d'adn ciblees et complexe moleculaire destine a etre utilisee dans ce dernier
CN104651357B (zh) * 2014-05-13 2017-06-16 中国农业科学院生物技术研究所 鼠李糖诱导型启动子及其应用
GB201501081D0 (en) * 2015-01-22 2015-03-11 Cilian Ag Use of enzymes with a wide pH activity range as medicaments for promoting digestion
CN106676125B (zh) * 2015-11-05 2020-08-14 中国科学院天津工业生物技术研究所 一种包含麦芽糖启动子的载体以及麦芽糖启动子突变体
CN105734061B (zh) * 2016-03-25 2019-07-23 中国农业科学院生物技术研究所 从毕赤酵母中分离的鼠李糖诱导型启动子及其应用
CA3056801A1 (fr) 2017-04-28 2018-11-01 Agrospheres, Inc. Compositions et procedes pour l'encapsulation et l'administration evolutive de produits agrochimiques
US11624061B2 (en) 2017-04-28 2023-04-11 Agrospheres, Inc. Compositions and methods for enzyme immobilization
EP3444269A1 (fr) * 2017-08-17 2019-02-20 National Research Council of Canada Systèmes et méthodes pour la production de polypeptides de toxine diphterique
US11812743B2 (en) 2017-09-25 2023-11-14 Agrospheres, Inc. Compositions and methods for scalable production and delivery of biologicals
US20230383321A1 (en) 2020-05-15 2023-11-30 Basf Se Improved method for the production of isoprenoids
CN118401535A (zh) 2021-12-10 2024-07-26 巴斯夫欧洲公司 草铵膦衍生物的酶促脱氨基甲酰化
MX2024007022A (es) 2021-12-10 2024-06-19 Basf Se Actividad herbicida de alquilfosfinatos.
WO2023105080A1 (fr) 2021-12-10 2023-06-15 Basf Se Synthèse de glufosinate à l'aide d'un procédé à base d'hydantoïnase
CN114107342B (zh) * 2021-12-27 2024-01-26 黄山同兮生物科技有限公司 一种去除发酵液中乳糖的方法
WO2024126202A1 (fr) 2022-12-16 2024-06-20 Basf Se Nouvelles synthases d'acide éthylènediamine-n,n'-disuccinique (edds)
WO2024175643A1 (fr) 2023-02-23 2024-08-29 Basf Se Procédé de préparation de l-glufosinate à partir de cyanhydrine ou de dérivés de cyanhydrine

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5028530A (en) 1985-01-28 1991-07-02 Xoma Corporation AraB promoters and method of producing polypeptides, including cecropins, by microbiological techniques
US5576195A (en) 1985-11-01 1996-11-19 Xoma Corporation Vectors with pectate lyase signal sequence
US5837509A (en) * 1992-12-30 1998-11-17 Bioteknologisk Institut Recombinant lactic acid bacterium containing an inserted promoter and method of constructing same
CN1189185A (zh) * 1995-05-23 1998-07-29 国家农艺研究院 降解鼠李半乳糖醛酸聚糖ⅱ的酶和微生物
SE511524C2 (sv) * 1997-06-02 1999-10-11 Essum Ab Lactobacillus casei rhamnosus-stam samt farmaceutisk beredning för bekämpning av patogena tarmbakterier
DE19848129A1 (de) 1998-10-19 2000-04-20 Basf Ag Verfahren zur Herstellung chiraler Carbonsäuren aus Nitrilen mit Hilfe einer Nitrilase oder Mikroorganismen, die ein Gen für die Nitrilase enthalten
US6713288B1 (en) * 1999-09-28 2004-03-30 University Of Stuttgart Whole cell catalysts
EP1224296A1 (fr) 1999-10-29 2002-07-24 Basf Aktiengesellschaft Hydrolase de l-pantolactone et procede de production de d-pantolactone
EP1268823B1 (fr) 2000-03-24 2007-11-07 XOMA Technology Ltd. Procedes et cellules ameliores servant a exprimer des produits de proteine de recombinaison
CN1160451C (zh) * 2000-10-16 2004-08-04 鼎健生物科技食品股份有限公司 鼠李糖乳酸杆菌菌株及其用途

Also Published As

Publication number Publication date
PT1570060E (pt) 2007-09-13
CY1107772T1 (el) 2013-06-19
CN100445391C (zh) 2008-12-24
EP1570060A1 (fr) 2005-09-07
ATE368746T1 (de) 2007-08-15
AU2003288189B8 (en) 2009-06-25
EP1570060B1 (fr) 2007-08-01
JP2006507831A (ja) 2006-03-09
US20060014291A1 (en) 2006-01-19
CN1720328A (zh) 2006-01-11
ES2288630T3 (es) 2008-01-16
AU2003288189A1 (en) 2004-06-23
JP4619126B2 (ja) 2011-01-26
DE50307835D1 (de) 2007-09-13
WO2004050877A1 (fr) 2004-06-17
DK1570060T3 (da) 2007-11-05
CA2506999A1 (fr) 2004-06-17
US8895310B2 (en) 2014-11-25
AU2003288189B2 (en) 2009-05-28

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