CA2492048A1 - Detection of chemical ligation using fluorescence quenching leaving groups - Google Patents
Detection of chemical ligation using fluorescence quenching leaving groups Download PDFInfo
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- CA2492048A1 CA2492048A1 CA002492048A CA2492048A CA2492048A1 CA 2492048 A1 CA2492048 A1 CA 2492048A1 CA 002492048 A CA002492048 A CA 002492048A CA 2492048 A CA2492048 A CA 2492048A CA 2492048 A1 CA2492048 A1 CA 2492048A1
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- nucleic acid
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- fluorescence
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- 230000035945 sensitivity Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 125000004354 sulfur functional group Chemical group 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 229940124597 therapeutic agent Drugs 0.000 description 1
- 150000007944 thiolates Chemical group 0.000 description 1
- 150000003573 thiols Chemical class 0.000 description 1
- 229940104230 thymidine Drugs 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 125000002088 tosyl group Chemical group [H]C1=C([H])C(=C([H])C([H])=C1C([H])([H])[H])S(*)(=O)=O 0.000 description 1
- 238000001890 transfection Methods 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- 229910052722 tritium Inorganic materials 0.000 description 1
- 201000008827 tuberculosis Diseases 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
- 229940035893 uracil Drugs 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6823—Release of bound markers
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US39677402P | 2002-07-18 | 2002-07-18 | |
| US60/396,774 | 2002-07-18 | ||
| PCT/US2003/022320 WO2004010101A2 (en) | 2002-07-18 | 2003-07-17 | Detection of chemical ligation using fluorescence quenching leaving groups |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2492048A1 true CA2492048A1 (en) | 2004-01-29 |
Family
ID=30770949
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002492048A Abandoned CA2492048A1 (en) | 2002-07-18 | 2003-07-17 | Detection of chemical ligation using fluorescence quenching leaving groups |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US7749699B2 (cg-RX-API-DMAC7.html) |
| EP (1) | EP1543156A4 (cg-RX-API-DMAC7.html) |
| JP (1) | JP2006510348A (cg-RX-API-DMAC7.html) |
| AU (1) | AU2003256587A1 (cg-RX-API-DMAC7.html) |
| CA (1) | CA2492048A1 (cg-RX-API-DMAC7.html) |
| WO (1) | WO2004010101A2 (cg-RX-API-DMAC7.html) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7033753B1 (en) * | 1999-01-15 | 2006-04-25 | University Of Rochester | Compositions and methods for nonenzymatic ligation of oligonucleotides and detection of genetic polymorphisms |
| US7745614B2 (en) | 2004-09-03 | 2010-06-29 | The Board Of Trustees Of The Leland Stanford Junior University | Universal linker compositions for the release or transfer of chemical agents from a polynucleotide |
| BRPI0611474A2 (pt) * | 2005-05-26 | 2010-09-14 | Ensemble Discovery Corp | biodetecção por quìmica modelada com ácido nucléico |
| WO2007133703A2 (en) * | 2006-05-10 | 2007-11-22 | Dxterity Diagnostics | Detection of nucleic acid targets using chemically reactive oligonucleotide probes |
| JP2008259453A (ja) * | 2007-04-12 | 2008-10-30 | Toyobo Co Ltd | 核酸の検出方法 |
| SG173627A1 (en) | 2009-02-13 | 2011-09-29 | Chem Inc X | Methods of creating and screening dna-encoded libraries |
| WO2010114599A1 (en) * | 2009-04-01 | 2010-10-07 | Dx Terity Diagnostics Inc. | Chemical ligation dependent probe amplification (clpa) |
| US10066257B2 (en) | 2010-03-29 | 2018-09-04 | Dxterity Diagnostics Incorporated | Methods and compositions for detecting target nucleic acids |
| US9845495B2 (en) | 2010-12-10 | 2017-12-19 | Abbott Laboratories | Method and kit for detecting target nucleic acid |
| US20140170653A1 (en) | 2011-04-15 | 2014-06-19 | Life Technologies Corporation | Chemical ligation |
| DK2748357T3 (en) | 2011-09-07 | 2018-07-16 | X Chem Inc | Methods for labeling DNA-encoded libraries |
| KR102146721B1 (ko) | 2012-07-13 | 2020-08-21 | 엑스-켐, 인크. | 폴리머라제에 의해 판독가능하지 않은 코딩 올리고뉴클레오티드 연결을 갖는 dna-코딩된 라이브러리 |
| CN105659091A (zh) | 2013-08-19 | 2016-06-08 | 卓异生物公司 | 用于单分子检测的测定及其应用 |
| CA2936157C (en) | 2014-01-15 | 2021-08-24 | Tokyo Institute Of Technology | Covered sequence conversion dna and detection methods |
| WO2015191777A2 (en) | 2014-06-10 | 2015-12-17 | Dxterity Diagnostics Incorporated | Devices and methods for collecting and stabilizing biological samples |
| CN107075585B (zh) * | 2014-10-14 | 2021-07-20 | 雅培实验室 | 具有锁核酸的序列转换和信号扩增dna以及使用其的检测方法 |
| ES2791280T3 (es) | 2014-12-24 | 2020-11-03 | Abbott Lab | Método de detección, composición y kit basados en reacciones en cascada de ADN amplificador de señal con extensión de la diana |
| WO2016134191A1 (en) | 2015-02-18 | 2016-08-25 | Singular Bio, Inc. | Assays for single molecule detection and use thereof |
| CN108350487B (zh) * | 2015-11-19 | 2022-05-27 | 加利福尼亚太平洋生物科学股份有限公司 | 用于改善信号检测的化合物和系统 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5476925A (en) * | 1993-02-01 | 1995-12-19 | Northwestern University | Oligodeoxyribonucleotides including 3'-aminonucleoside-phosphoramidate linkages and terminal 3'-amino groups |
| ES2204913T3 (es) * | 1993-04-12 | 2004-05-01 | Northwestern University | Metodo para formacion de oligonucleotidos. |
| US5681943A (en) * | 1993-04-12 | 1997-10-28 | Northwestern University | Method for covalently linking adjacent oligonucleotides |
| US5925517A (en) * | 1993-11-12 | 1999-07-20 | The Public Health Research Institute Of The City Of New York, Inc. | Detectably labeled dual conformation oligonucleotide probes, assays and kits |
| CA2219891C (en) * | 1995-05-05 | 2002-01-29 | Applied Biosystems, Llc | Methods and reagents for combined pcr amplification and hybridization probing assay |
| US6080868A (en) * | 1998-01-23 | 2000-06-27 | The Perkin-Elmer Corporation | Nitro-substituted non-fluorescent asymmetric cyanine dye compounds |
| US5952202A (en) * | 1998-03-26 | 1999-09-14 | The Perkin Elmer Corporation | Methods using exogenous, internal controls and analogue blocks during nucleic acid amplification |
-
2003
- 2003-07-17 JP JP2004523488A patent/JP2006510348A/ja active Pending
- 2003-07-17 EP EP03765644A patent/EP1543156A4/en not_active Withdrawn
- 2003-07-17 CA CA002492048A patent/CA2492048A1/en not_active Abandoned
- 2003-07-17 US US10/604,400 patent/US7749699B2/en not_active Expired - Fee Related
- 2003-07-17 WO PCT/US2003/022320 patent/WO2004010101A2/en not_active Ceased
- 2003-07-17 AU AU2003256587A patent/AU2003256587A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US20040259102A1 (en) | 2004-12-23 |
| EP1543156A2 (en) | 2005-06-22 |
| AU2003256587A1 (en) | 2004-02-09 |
| EP1543156A4 (en) | 2005-12-21 |
| WO2004010101A2 (en) | 2004-01-29 |
| AU2003256587A8 (en) | 2004-02-09 |
| US7749699B2 (en) | 2010-07-06 |
| WO2004010101A3 (en) | 2004-09-02 |
| JP2006510348A (ja) | 2006-03-30 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| FZDE | Discontinued |