AU2003256587A1 - Detection of chemical ligation using fluorescence quenching leaving groups - Google Patents

Detection of chemical ligation using fluorescence quenching leaving groups

Info

Publication number
AU2003256587A1
AU2003256587A1 AU2003256587A AU2003256587A AU2003256587A1 AU 2003256587 A1 AU2003256587 A1 AU 2003256587A1 AU 2003256587 A AU2003256587 A AU 2003256587A AU 2003256587 A AU2003256587 A AU 2003256587A AU 2003256587 A1 AU2003256587 A1 AU 2003256587A1
Authority
AU
Australia
Prior art keywords
detection
leaving groups
fluorescence quenching
chemical ligation
quenching leaving
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2003256587A
Other languages
English (en)
Other versions
AU2003256587A8 (en
Inventor
Eric T. Kool
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Leland Stanford Junior University
Original Assignee
Leland Stanford Junior University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Leland Stanford Junior University filed Critical Leland Stanford Junior University
Publication of AU2003256587A1 publication Critical patent/AU2003256587A1/en
Publication of AU2003256587A8 publication Critical patent/AU2003256587A8/xx
Abandoned legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2003256587A 2002-07-18 2003-07-17 Detection of chemical ligation using fluorescence quenching leaving groups Abandoned AU2003256587A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US39677402P 2002-07-18 2002-07-18
US60/396,774 2002-07-18
PCT/US2003/022320 WO2004010101A2 (en) 2002-07-18 2003-07-17 Detection of chemical ligation using fluorescence quenching leaving groups

Publications (2)

Publication Number Publication Date
AU2003256587A1 true AU2003256587A1 (en) 2004-02-09
AU2003256587A8 AU2003256587A8 (en) 2004-02-09

Family

ID=30770949

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2003256587A Abandoned AU2003256587A1 (en) 2002-07-18 2003-07-17 Detection of chemical ligation using fluorescence quenching leaving groups

Country Status (6)

Country Link
US (1) US7749699B2 (cg-RX-API-DMAC7.html)
EP (1) EP1543156A4 (cg-RX-API-DMAC7.html)
JP (1) JP2006510348A (cg-RX-API-DMAC7.html)
AU (1) AU2003256587A1 (cg-RX-API-DMAC7.html)
CA (1) CA2492048A1 (cg-RX-API-DMAC7.html)
WO (1) WO2004010101A2 (cg-RX-API-DMAC7.html)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7033753B1 (en) * 1999-01-15 2006-04-25 University Of Rochester Compositions and methods for nonenzymatic ligation of oligonucleotides and detection of genetic polymorphisms
US7745614B2 (en) 2004-09-03 2010-06-29 The Board Of Trustees Of The Leland Stanford Junior University Universal linker compositions for the release or transfer of chemical agents from a polynucleotide
BRPI0611474A2 (pt) * 2005-05-26 2010-09-14 Ensemble Discovery Corp biodetecção por quìmica modelada com ácido nucléico
WO2007133703A2 (en) * 2006-05-10 2007-11-22 Dxterity Diagnostics Detection of nucleic acid targets using chemically reactive oligonucleotide probes
JP2008259453A (ja) * 2007-04-12 2008-10-30 Toyobo Co Ltd 核酸の検出方法
SG173627A1 (en) 2009-02-13 2011-09-29 Chem Inc X Methods of creating and screening dna-encoded libraries
WO2010114599A1 (en) * 2009-04-01 2010-10-07 Dx Terity Diagnostics Inc. Chemical ligation dependent probe amplification (clpa)
US10066257B2 (en) 2010-03-29 2018-09-04 Dxterity Diagnostics Incorporated Methods and compositions for detecting target nucleic acids
US9845495B2 (en) 2010-12-10 2017-12-19 Abbott Laboratories Method and kit for detecting target nucleic acid
US20140170653A1 (en) 2011-04-15 2014-06-19 Life Technologies Corporation Chemical ligation
DK2748357T3 (en) 2011-09-07 2018-07-16 X Chem Inc Methods for labeling DNA-encoded libraries
KR102146721B1 (ko) 2012-07-13 2020-08-21 엑스-켐, 인크. 폴리머라제에 의해 판독가능하지 않은 코딩 올리고뉴클레오티드 연결을 갖는 dna-코딩된 라이브러리
CN105659091A (zh) 2013-08-19 2016-06-08 卓异生物公司 用于单分子检测的测定及其应用
CA2936157C (en) 2014-01-15 2021-08-24 Tokyo Institute Of Technology Covered sequence conversion dna and detection methods
WO2015191777A2 (en) 2014-06-10 2015-12-17 Dxterity Diagnostics Incorporated Devices and methods for collecting and stabilizing biological samples
CN107075585B (zh) * 2014-10-14 2021-07-20 雅培实验室 具有锁核酸的序列转换和信号扩增dna以及使用其的检测方法
ES2791280T3 (es) 2014-12-24 2020-11-03 Abbott Lab Método de detección, composición y kit basados en reacciones en cascada de ADN amplificador de señal con extensión de la diana
WO2016134191A1 (en) 2015-02-18 2016-08-25 Singular Bio, Inc. Assays for single molecule detection and use thereof
CN108350487B (zh) * 2015-11-19 2022-05-27 加利福尼亚太平洋生物科学股份有限公司 用于改善信号检测的化合物和系统

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5476925A (en) * 1993-02-01 1995-12-19 Northwestern University Oligodeoxyribonucleotides including 3'-aminonucleoside-phosphoramidate linkages and terminal 3'-amino groups
ES2204913T3 (es) * 1993-04-12 2004-05-01 Northwestern University Metodo para formacion de oligonucleotidos.
US5681943A (en) * 1993-04-12 1997-10-28 Northwestern University Method for covalently linking adjacent oligonucleotides
US5925517A (en) * 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
CA2219891C (en) * 1995-05-05 2002-01-29 Applied Biosystems, Llc Methods and reagents for combined pcr amplification and hybridization probing assay
US6080868A (en) * 1998-01-23 2000-06-27 The Perkin-Elmer Corporation Nitro-substituted non-fluorescent asymmetric cyanine dye compounds
US5952202A (en) * 1998-03-26 1999-09-14 The Perkin Elmer Corporation Methods using exogenous, internal controls and analogue blocks during nucleic acid amplification

Also Published As

Publication number Publication date
US20040259102A1 (en) 2004-12-23
EP1543156A2 (en) 2005-06-22
CA2492048A1 (en) 2004-01-29
EP1543156A4 (en) 2005-12-21
WO2004010101A2 (en) 2004-01-29
AU2003256587A8 (en) 2004-02-09
US7749699B2 (en) 2010-07-06
WO2004010101A3 (en) 2004-09-02
JP2006510348A (ja) 2006-03-30

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Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase