CA2489520A1 - A method for the detection of apoptosis - Google Patents
A method for the detection of apoptosis Download PDFInfo
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- CA2489520A1 CA2489520A1 CA002489520A CA2489520A CA2489520A1 CA 2489520 A1 CA2489520 A1 CA 2489520A1 CA 002489520 A CA002489520 A CA 002489520A CA 2489520 A CA2489520 A CA 2489520A CA 2489520 A1 CA2489520 A1 CA 2489520A1
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- nucleolin
- detecting
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- antibody
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Classifications
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6875—Nucleoproteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/25—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/564—Immunoassay; Biospecific binding assay; Materials therefor for pre-existing immune complex or autoimmune disease, i.e. systemic lupus erythematosus, rheumatoid arthritis, multiple sclerosis, rheumatoid factors or complement components C1-C9
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
- G01N33/57488—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites involving compounds identifable in body fluids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
- G01N33/6896—Neurological disorders, e.g. Alzheimer's disease
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/91—Transferases (2.)
- G01N2333/91091—Glycosyltransferases (2.4)
- G01N2333/91142—Pentosyltransferases (2.4.2)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2510/00—Detection of programmed cell death, i.e. apoptosis
Landscapes
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
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- General Health & Medical Sciences (AREA)
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- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
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- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Rehabilitation Therapy (AREA)
- Neurology (AREA)
- Rheumatology (AREA)
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- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Hospice & Palliative Care (AREA)
- Oncology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
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| US39214302P | 2002-06-26 | 2002-06-26 | |
| US60/392,143 | 2002-06-26 | ||
| PCT/US2003/020167 WO2004003554A1 (en) | 2002-06-26 | 2003-06-26 | A method for the detection of apoptosis |
Publications (1)
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|---|---|
| CA2489520A1 true CA2489520A1 (en) | 2004-01-08 |
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|---|---|---|---|
| CA002489520A Abandoned CA2489520A1 (en) | 2002-06-26 | 2003-06-26 | A method for the detection of apoptosis |
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| US (2) | US20040132049A1 (enExample) |
| EP (1) | EP1516186B1 (enExample) |
| JP (1) | JP2005530515A (enExample) |
| AT (1) | ATE458196T1 (enExample) |
| AU (2) | AU2003248724A1 (enExample) |
| CA (1) | CA2489520A1 (enExample) |
| DE (1) | DE60331310D1 (enExample) |
| WO (1) | WO2004003554A1 (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9452219B2 (en) | 2011-06-02 | 2016-09-27 | University Of Louisville Research Foundation, Inc. | Anti-nucleolin agent-conjugated nanoparticles |
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| US8114850B2 (en) * | 1999-04-08 | 2012-02-14 | Advanced Cancer Therapeutics, Llc | Antiproliferative activity of G-rich oligonucleotides and method of using same to bind to nucleolin |
| WO2000061597A1 (en) | 1999-04-08 | 2000-10-19 | Uab Research Foundation | Antiproliferative activity of g-righ oligonucleotides and method of using same to bind to nucleolin |
| US7960540B2 (en) * | 1999-04-08 | 2011-06-14 | Advanced Cancer Therapeutics, Llc | Antiproliferative activity of G-rich oligonucleotides and method of using same to bind to nucleolin |
| US20110178161A1 (en) * | 1999-04-08 | 2011-07-21 | Antisoma Research Limited | Antiproliferative activity of G-rich oligonucleotides and method of using same to bind to nucleolin |
| US7541150B2 (en) | 2002-04-08 | 2009-06-02 | University Of Louisville Research Foundation, Inc | Method for the diagnosis and prognosis of malignant diseases |
| US7357928B2 (en) | 2002-04-08 | 2008-04-15 | University Of Louisville Research Foundation, Inc. | Method for the diagnosis and prognosis of malignant diseases |
| KR20050114217A (ko) * | 2003-02-21 | 2005-12-05 | 메드벳 싸이언스 피티와이. 리미티드 | 진단 및 치료 방법 |
| JP5025724B2 (ja) * | 2006-04-18 | 2012-09-12 | ウェルスタット バイオロジクス コーポレイション | 循環腫瘍性細胞からのタンパク質の検出 |
| WO2008076870A2 (en) * | 2006-12-14 | 2008-06-26 | Cady Roger K | Method and system for interactive cognitive testing |
| US20090131351A1 (en) * | 2007-11-16 | 2009-05-21 | Antisoma Research Limited | Methods, compositions, and kits for modulating tumor cell proliferation |
| JP4924415B2 (ja) * | 2007-12-28 | 2012-04-25 | 株式会社日立プラントテクノロジー | アポトーシス検出方法及び装置 |
| US20090226914A1 (en) * | 2007-12-31 | 2009-09-10 | Bates Paula J | Methods and products to target, capture and characterize stem cells |
| JP6174320B2 (ja) | 2009-11-17 | 2017-08-02 | エムユーエスシー ファウンデーション フォー リサーチ ディベロップメント | ヒトヌクレオリンに対するヒトモノクローナル抗体 |
| US20130052647A1 (en) * | 2010-02-10 | 2013-02-28 | Marianna Goldrick | Methods for fractionating and processing microparticles from biological samples and using them for biomarker discovery |
| UA105278C2 (ru) | 2012-09-06 | 2014-04-25 | Інститут Біології Клітини Нан України | Способ получения каталитически активных антител (абзимов) с сиалидазной активностью |
| EP3291839A1 (en) | 2015-05-05 | 2018-03-14 | The University of Louisville Research Foundation, Inc. | Anti-nucleolin agent-conjugated nanoparticles as radio-sensitizers and mri and/or x-ray contrast agents |
| CN111579538B (zh) * | 2020-04-22 | 2022-12-30 | 山东第一医科大学(山东省医学科学院) | 一种利用凋亡试剂盒检测循环肿瘤细胞的方法 |
| CN117054200B (zh) * | 2023-07-21 | 2024-11-26 | 伊莱瑞特(武汉)生物技术有限公司 | 用于流式细胞术分析凋亡细胞的tunel专用固定破膜剂及其制备方法与应用 |
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|---|---|---|---|---|
| US4522811A (en) * | 1982-07-08 | 1985-06-11 | Syntex (U.S.A.) Inc. | Serial injection of muramyldipeptides and liposomes enhances the anti-infective activity of muramyldipeptides |
| US4474892A (en) * | 1983-02-16 | 1984-10-02 | Board Of Trustees Of The Leland Stanford Junior University | Two-site immunoassays using monoclonal antibodies of different classes or subclasses and test kits for performing same |
| US5169774A (en) * | 1984-02-08 | 1992-12-08 | Cetus Oncology Corporation | Monoclonal anti-human breast cancer antibodies |
| US4671256A (en) * | 1984-05-25 | 1987-06-09 | Lemelson Jerome H | Medical scanning, monitoring and treatment system and method |
| US5055459A (en) * | 1986-06-30 | 1991-10-08 | Board Of Regents, The University Of Texas | Selective elimination of malignant cells from bone marrow by bis (acyloxy) propylphosphoramidates |
| US4987071A (en) * | 1986-12-03 | 1991-01-22 | University Patents, Inc. | RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods |
| GB8823869D0 (en) * | 1988-10-12 | 1988-11-16 | Medical Res Council | Production of antibodies |
| US5530101A (en) * | 1988-12-28 | 1996-06-25 | Protein Design Labs, Inc. | Humanized immunoglobulins |
| US5192660A (en) * | 1989-04-24 | 1993-03-09 | The United States Of America As Represented By The Department Of Health And Human Services | ELISA methods for the determination of human platelet derived growth factor (PDGF) dimer forms present in human tissues and fluids |
| US6020145A (en) * | 1989-06-30 | 2000-02-01 | Bristol-Myers Squibb Company | Methods for determining the presence of carcinoma using the antigen binding region of monoclonal antibody BR96 |
| AU635008B2 (en) * | 1989-12-13 | 1993-03-11 | Genelabs Diagnostics Pte Ltd | Analytical apparatus and method for automated blot assay |
| US6657103B1 (en) * | 1990-01-12 | 2003-12-02 | Abgenix, Inc. | Human antibodies derived from immunized xenomice |
| US5770429A (en) * | 1990-08-29 | 1998-06-23 | Genpharm International, Inc. | Transgenic non-human animals capable of producing heterologous antibodies |
| US5736348A (en) * | 1990-11-28 | 1998-04-07 | Fundacao Oswaldo Cruz (Fiocruz) | Method for the immunological diagnosis of Chagas' Disease using recombinant antigens |
| US5691447A (en) * | 1995-03-24 | 1997-11-25 | Tanox Biosystems, Inc. | GC1q receptor, HIV-1 gp120 region binding thereto, and related peptides and targeting antibodies |
| US6096532A (en) * | 1995-06-07 | 2000-08-01 | Aastrom Biosciences, Inc. | Processor apparatus for use in a system for maintaining and growing biological cells |
| US6048703A (en) * | 1996-11-15 | 2000-04-11 | Cephalon, Inc. | Methods for detecting cell apoptosis |
| WO1998040480A1 (en) * | 1997-03-12 | 1998-09-17 | Institut Pasteur | A novel cell surface receptor for hiv retroviruses, therapeutic and diagnostic uses |
| CA2293724C (en) * | 1997-06-05 | 2010-02-02 | Xiaodong Wang | Apaf-1, the ced-4 human homolog, an activator of caspase-3 |
| EP1958962A3 (en) * | 1997-06-12 | 2013-05-01 | Novartis International Pharmaceutical Ltd. | Artificial antibody polypeptides |
| US6339075B1 (en) * | 1997-06-30 | 2002-01-15 | The University Of British Columbia | Use of dextran and other polysaccharides to improve mucus clearance |
| AU6053898A (en) * | 1997-08-04 | 1999-02-22 | Burnham Institute, The | A cell-free system of mitochondria-dependent apoptosis, and methods of use ther eof |
| US6325785B1 (en) * | 1997-08-14 | 2001-12-04 | Sherwood Services Ag | Sputum trap manifold with nested caps |
| US5925334A (en) * | 1997-08-27 | 1999-07-20 | Rubin; Bruce K. | Use of surface active agents to promote mucus clearance |
| US5932475A (en) * | 1997-12-12 | 1999-08-03 | Incyte Pharmaceuticals, Inc. | Human nucleolin-like protein |
| US6506559B1 (en) * | 1997-12-23 | 2003-01-14 | Carnegie Institute Of Washington | Genetic inhibition by double-stranded RNA |
| US6306404B1 (en) * | 1998-07-14 | 2001-10-23 | American Cyanamid Company | Adjuvant and vaccine compositions containing monophosphoryl lipid A |
| WO2000017648A1 (en) * | 1998-09-24 | 2000-03-30 | Promega Corporation | Apoptosis marker antibodies and methods of use |
| CA2363984A1 (en) * | 1999-04-15 | 2000-10-26 | Merck Frosst Canada & Co. | Antibodies that recognize app cleaved by caspases and methods of use |
| JP3869722B2 (ja) * | 1999-11-08 | 2007-01-17 | エーザイ・アール・アンド・ディー・マネジメント株式会社 | 細胞死の検出方法及び検出試薬 |
| US20020028488A1 (en) * | 2000-06-19 | 2002-03-07 | Sujay Singh | Transgenic avian species for making human and chimeric antibodies |
| US7357928B2 (en) * | 2002-04-08 | 2008-04-15 | University Of Louisville Research Foundation, Inc. | Method for the diagnosis and prognosis of malignant diseases |
| US7541150B2 (en) * | 2002-04-08 | 2009-06-02 | University Of Louisville Research Foundation, Inc | Method for the diagnosis and prognosis of malignant diseases |
| US20110011002A1 (en) * | 2006-01-17 | 2011-01-20 | Christian Guillemette | Semi-removable window frame assembly |
| CA2774361C (en) * | 2009-10-21 | 2018-04-24 | Health Research, Inc. | Paa nanoparticles for enhancement of tumor imaging |
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- 2003-06-26 AT AT03762073T patent/ATE458196T1/de not_active IP Right Cessation
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- 2003-06-26 US US10/607,455 patent/US20040132049A1/en not_active Abandoned
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2010
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9452219B2 (en) | 2011-06-02 | 2016-09-27 | University Of Louisville Research Foundation, Inc. | Anti-nucleolin agent-conjugated nanoparticles |
Also Published As
| Publication number | Publication date |
|---|---|
| US20040132049A1 (en) | 2004-07-08 |
| US20110065121A1 (en) | 2011-03-17 |
| DE60331310D1 (de) | 2010-04-01 |
| ATE458196T1 (de) | 2010-03-15 |
| EP1516186A4 (en) | 2006-11-22 |
| EP1516186A1 (en) | 2005-03-23 |
| JP2005530515A (ja) | 2005-10-13 |
| AU2010202118A1 (en) | 2010-06-17 |
| AU2003248724A1 (en) | 2004-01-19 |
| EP1516186B1 (en) | 2010-02-17 |
| WO2004003554A1 (en) | 2004-01-08 |
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| EEER | Examination request | ||
| FZDE | Discontinued |