CA2484730A1 - Device and method for analytical cell imaging - Google Patents
Device and method for analytical cell imaging Download PDFInfo
- Publication number
- CA2484730A1 CA2484730A1 CA002484730A CA2484730A CA2484730A1 CA 2484730 A1 CA2484730 A1 CA 2484730A1 CA 002484730 A CA002484730 A CA 002484730A CA 2484730 A CA2484730 A CA 2484730A CA 2484730 A1 CA2484730 A1 CA 2484730A1
- Authority
- CA
- Canada
- Prior art keywords
- cells
- illumination
- sample
- group
- sample chamber
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000000034 method Methods 0.000 title claims description 42
- 238000003384 imaging method Methods 0.000 title claims description 27
- 210000004027 cell Anatomy 0.000 claims abstract description 133
- 238000004458 analytical method Methods 0.000 claims abstract description 30
- 210000002919 epithelial cell Anatomy 0.000 claims abstract description 16
- 208000005443 Circulating Neoplastic Cells Diseases 0.000 claims abstract description 10
- 239000000523 sample Substances 0.000 claims description 62
- 238000005286 illumination Methods 0.000 claims description 39
- 238000001514 detection method Methods 0.000 claims description 30
- 239000012472 biological sample Substances 0.000 claims description 17
- 210000001519 tissue Anatomy 0.000 claims description 7
- 239000000126 substance Substances 0.000 claims description 6
- 108020004707 nucleic acids Proteins 0.000 claims description 5
- 102000039446 nucleic acids Human genes 0.000 claims description 5
- 150000007523 nucleic acids Chemical class 0.000 claims description 5
- 210000005259 peripheral blood Anatomy 0.000 claims description 5
- 239000011886 peripheral blood Substances 0.000 claims description 5
- 241000894006 Bacteria Species 0.000 claims description 3
- 208000005228 Pericardial Effusion Diseases 0.000 claims description 3
- 206010036790 Productive cough Diseases 0.000 claims description 3
- 210000003567 ascitic fluid Anatomy 0.000 claims description 3
- 230000000112 colonic effect Effects 0.000 claims description 3
- 239000005556 hormone Substances 0.000 claims description 3
- 229940088597 hormone Drugs 0.000 claims description 3
- 210000002445 nipple Anatomy 0.000 claims description 3
- 210000004912 pericardial fluid Anatomy 0.000 claims description 3
- 210000004910 pleural fluid Anatomy 0.000 claims description 3
- 210000003802 sputum Anatomy 0.000 claims description 3
- 208000024794 sputum Diseases 0.000 claims description 3
- 210000002700 urine Anatomy 0.000 claims description 3
- 102000004856 Lectins Human genes 0.000 claims description 2
- 108090001090 Lectins Proteins 0.000 claims description 2
- 108091034117 Oligonucleotide Proteins 0.000 claims description 2
- 241000700605 Viruses Species 0.000 claims description 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims description 2
- 239000003814 drug Substances 0.000 claims description 2
- 229940079593 drug Drugs 0.000 claims description 2
- 230000001605 fetal effect Effects 0.000 claims description 2
- 239000002523 lectin Substances 0.000 claims description 2
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 2
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 2
- 102000004169 proteins and genes Human genes 0.000 claims description 2
- 108090000623 proteins and genes Proteins 0.000 claims description 2
- 210000001185 bone marrow Anatomy 0.000 claims 2
- 210000002358 circulating endothelial cell Anatomy 0.000 claims 1
- 210000000130 stem cell Anatomy 0.000 claims 1
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 abstract description 73
- 229910052759 nickel Inorganic materials 0.000 abstract description 36
- 210000004369 blood Anatomy 0.000 abstract description 25
- 239000008280 blood Substances 0.000 abstract description 25
- 210000004881 tumor cell Anatomy 0.000 abstract description 22
- 230000005291 magnetic effect Effects 0.000 abstract description 13
- 206010028980 Neoplasm Diseases 0.000 abstract description 10
- 201000009030 Carcinoma Diseases 0.000 abstract description 5
- 230000035945 sensitivity Effects 0.000 abstract description 5
- 238000001574 biopsy Methods 0.000 abstract 1
- 238000012512 characterization method Methods 0.000 abstract 1
- 201000010099 disease Diseases 0.000 abstract 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract 1
- 238000001444 catalytic combustion detection Methods 0.000 description 15
- 210000000265 leukocyte Anatomy 0.000 description 12
- 230000003287 optical effect Effects 0.000 description 12
- FWBHETKCLVMNFS-UHFFFAOYSA-N 4',6-Diamino-2-phenylindol Chemical compound C1=CC(C(=N)N)=CC=C1C1=CC2=CC=C(C(N)=N)C=C2N1 FWBHETKCLVMNFS-UHFFFAOYSA-N 0.000 description 8
- 201000011510 cancer Diseases 0.000 description 7
- 230000000875 corresponding effect Effects 0.000 description 6
- 238000009826 distribution Methods 0.000 description 6
- 239000003446 ligand Substances 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 6
- 230000003068 static effect Effects 0.000 description 6
- 102000011782 Keratins Human genes 0.000 description 5
- 108010076876 Keratins Proteins 0.000 description 5
- 238000009739 binding Methods 0.000 description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 230000001427 coherent effect Effects 0.000 description 5
- 230000005284 excitation Effects 0.000 description 5
- 210000004940 nucleus Anatomy 0.000 description 5
- 238000010186 staining Methods 0.000 description 5
- 101000738771 Homo sapiens Receptor-type tyrosine-protein phosphatase C Proteins 0.000 description 4
- 102100037422 Receptor-type tyrosine-protein phosphatase C Human genes 0.000 description 4
- 230000027455 binding Effects 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000011554 ferrofluid Substances 0.000 description 4
- 239000012530 fluid Substances 0.000 description 4
- 238000002546 full scan Methods 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 230000009870 specific binding Effects 0.000 description 4
- 102000018651 Epithelial Cell Adhesion Molecule Human genes 0.000 description 3
- 108010066687 Epithelial Cell Adhesion Molecule Proteins 0.000 description 3
- 239000000427 antigen Substances 0.000 description 3
- 102000036639 antigens Human genes 0.000 description 3
- 108091007433 antigens Proteins 0.000 description 3
- 238000000684 flow cytometry Methods 0.000 description 3
- 150000002500 ions Chemical class 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 230000003595 spectral effect Effects 0.000 description 3
- 238000001228 spectrum Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 102000010831 Cytoskeletal Proteins Human genes 0.000 description 2
- 108010037414 Cytoskeletal Proteins Proteins 0.000 description 2
- DWJXYEABWRJFSP-XOBRGWDASA-N DAPT Chemical compound N([C@@H](C)C(=O)N[C@H](C(=O)OC(C)(C)C)C=1C=CC=CC=1)C(=O)CC1=CC(F)=CC(F)=C1 DWJXYEABWRJFSP-XOBRGWDASA-N 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 229960002685 biotin Drugs 0.000 description 2
- 239000011616 biotin Substances 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 238000005094 computer simulation Methods 0.000 description 2
- 238000012790 confirmation Methods 0.000 description 2
- 238000011977 dual antiplatelet therapy Methods 0.000 description 2
- 210000000981 epithelium Anatomy 0.000 description 2
- 238000000799 fluorescence microscopy Methods 0.000 description 2
- 238000001943 fluorescence-activated cell sorting Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 238000010191 image analysis Methods 0.000 description 2
- 230000008774 maternal effect Effects 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 238000007493 shaping process Methods 0.000 description 2
- 238000004088 simulation Methods 0.000 description 2
- 239000010409 thin film Substances 0.000 description 2
- 230000000007 visual effect Effects 0.000 description 2
- BUOYTFVLNZIELF-UHFFFAOYSA-N 2-phenyl-1h-indole-4,6-dicarboximidamide Chemical compound N1C2=CC(C(=N)N)=CC(C(N)=N)=C2C=C1C1=CC=CC=C1 BUOYTFVLNZIELF-UHFFFAOYSA-N 0.000 description 1
- 241001502050 Acis Species 0.000 description 1
- 238000012935 Averaging Methods 0.000 description 1
- 206010006187 Breast cancer Diseases 0.000 description 1
- 208000026310 Breast neoplasm Diseases 0.000 description 1
- 108010077544 Chromatin Proteins 0.000 description 1
- 208000017667 Chronic Disease Diseases 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 102000009109 Fc receptors Human genes 0.000 description 1
- 108010087819 Fc receptors Proteins 0.000 description 1
- 238000005481 NMR spectroscopy Methods 0.000 description 1
- 108010004729 Phycoerythrin Proteins 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 206010036618 Premenstrual syndrome Diseases 0.000 description 1
- 206010060862 Prostate cancer Diseases 0.000 description 1
- 208000037323 Rare tumor Diseases 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 102000018265 Virus Receptors Human genes 0.000 description 1
- 108010066342 Virus Receptors Proteins 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 108010004469 allophycocyanin Proteins 0.000 description 1
- 238000000627 alternating current impedance spectroscopy Methods 0.000 description 1
- 239000005557 antagonist Substances 0.000 description 1
- 238000003491 array Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 108010053098 biotin receptor Proteins 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- GEHJBWKLJVFKPS-UHFFFAOYSA-N bromochloroacetic acid Chemical compound OC(=O)C(Cl)Br GEHJBWKLJVFKPS-UHFFFAOYSA-N 0.000 description 1
- 230000006727 cell loss Effects 0.000 description 1
- 239000002771 cell marker Substances 0.000 description 1
- 210000003855 cell nucleus Anatomy 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 238000000701 chemical imaging Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 210000003483 chromatin Anatomy 0.000 description 1
- 238000004590 computer program Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000002380 cytological effect Effects 0.000 description 1
- 238000004163 cytometry Methods 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- BFMYDTVEBKDAKJ-UHFFFAOYSA-L disodium;(2',7'-dibromo-3',6'-dioxido-3-oxospiro[2-benzofuran-1,9'-xanthene]-4'-yl)mercury;hydrate Chemical compound O.[Na+].[Na+].O1C(=O)C2=CC=CC=C2C21C1=CC(Br)=C([O-])C([Hg])=C1OC1=C2C=C(Br)C([O-])=C1 BFMYDTVEBKDAKJ-UHFFFAOYSA-L 0.000 description 1
- 238000000295 emission spectrum Methods 0.000 description 1
- 210000002889 endothelial cell Anatomy 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000000695 excitation spectrum Methods 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 230000005294 ferromagnetic effect Effects 0.000 description 1
- 210000004700 fetal blood Anatomy 0.000 description 1
- 238000002073 fluorescence micrograph Methods 0.000 description 1
- 238000012632 fluorescent imaging Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 238000010166 immunofluorescence Methods 0.000 description 1
- 238000013394 immunophenotyping Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 230000036210 malignancy Effects 0.000 description 1
- 230000003211 malignant effect Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000007431 microscopic evaluation Methods 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000003068 molecular probe Substances 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 230000009871 nonspecific binding Effects 0.000 description 1
- 238000012758 nuclear staining Methods 0.000 description 1
- 230000005298 paramagnetic effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000013610 patient sample Substances 0.000 description 1
- 238000003909 pattern recognition Methods 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 230000035935 pregnancy Effects 0.000 description 1
- 230000000135 prohibitive effect Effects 0.000 description 1
- 201000001514 prostate carcinoma Diseases 0.000 description 1
- 210000005267 prostate cell Anatomy 0.000 description 1
- 238000006862 quantum yield reaction Methods 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 239000002195 soluble material Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000010183 spectrum analysis Methods 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000000207 volumetry Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1429—Signal processing
- G01N15/1433—Signal processing using image recognition
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
- G01N33/57492—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites involving compounds localized on the membrane of tumor or cancer cells
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Hematology (AREA)
- Cell Biology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Oncology (AREA)
- Signal Processing (AREA)
- Optics & Photonics (AREA)
- Hospice & Palliative Care (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Dispersion Chemistry (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US37786802P | 2002-05-03 | 2002-05-03 | |
| US60/377,868 | 2002-05-03 | ||
| PCT/US2003/013842 WO2003093795A2 (en) | 2002-05-03 | 2003-05-05 | Device and method for analytical cell imaging |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2484730A1 true CA2484730A1 (en) | 2003-11-13 |
Family
ID=29401578
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002484730A Abandoned CA2484730A1 (en) | 2002-05-03 | 2003-05-05 | Device and method for analytical cell imaging |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP1501917A4 (de) |
| JP (1) | JP2005524833A (de) |
| AU (1) | AU2003234459A1 (de) |
| CA (1) | CA2484730A1 (de) |
| WO (1) | WO2003093795A2 (de) |
Families Citing this family (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2359689B1 (de) | 2002-09-27 | 2015-08-26 | The General Hospital Corporation | Mikrofluid-Vorrichtung zur Zelltrennung, sowie Verwendung derselben |
| JP4041458B2 (ja) * | 2003-12-12 | 2008-01-30 | 日立ソフトウエアエンジニアリング株式会社 | 蛍光ビーズ検出方法及び蛍光ビーズ検出装置 |
| WO2006017811A2 (en) | 2004-08-06 | 2006-02-16 | Compucyte Corporation | Multiple-color monochromatic light absorption and quantification of light absorption in a stained sample |
| EP1810021A1 (de) * | 2004-10-22 | 2007-07-25 | Fermiscan Australia Pty Limited | Analyseverfahren und -vorrichtung |
| US20070196820A1 (en) | 2005-04-05 | 2007-08-23 | Ravi Kapur | Devices and methods for enrichment and alteration of cells and other particles |
| US8921102B2 (en) | 2005-07-29 | 2014-12-30 | Gpb Scientific, Llc | Devices and methods for enrichment and alteration of circulating tumor cells and other particles |
| ES2886923T3 (es) | 2005-09-20 | 2021-12-21 | Menarini Silicon Biosystems Spa | Métodos y composición para generar sondas de ADN de secuencia única, marcaje de sondas de ADN y el uso de estas sondas |
| US9134237B2 (en) | 2005-09-20 | 2015-09-15 | Janssen Diagnotics, LLC | High sensitivity multiparameter method for rare event analysis in a biological sample |
| FI20051329A0 (fi) | 2005-12-27 | 2005-12-27 | Wallac Oy | Laitteisto ja menetelmä näytteiden optista mittausta varten |
| CA2652027C (en) * | 2006-05-12 | 2017-06-06 | Immunivest Corporation | A laser illumination system in fluorescent microscopy |
| US8137912B2 (en) | 2006-06-14 | 2012-03-20 | The General Hospital Corporation | Methods for the diagnosis of fetal abnormalities |
| EP2029779A4 (de) | 2006-06-14 | 2010-01-20 | Living Microsystems Inc | Verwendung hoch paralleler snp-genotypisierung zur fötalen diagnose |
| US8372584B2 (en) | 2006-06-14 | 2013-02-12 | The General Hospital Corporation | Rare cell analysis using sample splitting and DNA tags |
| US20080050739A1 (en) | 2006-06-14 | 2008-02-28 | Roland Stoughton | Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats |
| WO2008012724A2 (en) * | 2006-07-20 | 2008-01-31 | Koninklijke Philips Electronics N.V. | Optical tracking and position determination for detection methods and systems |
| US7782452B2 (en) * | 2007-08-31 | 2010-08-24 | Kla-Tencor Technologies Corp. | Systems and method for simultaneously inspecting a specimen with two distinct channels |
| US8143600B2 (en) * | 2008-02-18 | 2012-03-27 | Visiongate, Inc. | 3D imaging of live cells with ultraviolet radiation |
| CA3069082C (en) | 2008-09-20 | 2022-03-22 | The Board Of Trustees Of The Leland Stanford Junior University | Noninvasive diagnosis of fetal aneuploidy by sequencing |
| JP5119114B2 (ja) * | 2008-09-30 | 2013-01-16 | 富士フイルム株式会社 | 光源装置及び撮影装置 |
| JP4805418B1 (ja) * | 2010-03-31 | 2011-11-02 | 古河電気工業株式会社 | 細胞の識別装置及び識別方法 |
| KR20150023022A (ko) | 2011-03-24 | 2015-03-04 | 앤팩 바이오-메디컬 사이언스 시오., 엘티디. | 질병 검출용 마이크로 소자 |
| WO2012151501A2 (en) * | 2011-05-05 | 2012-11-08 | Anpac Bio-Medical Science Co., Ltd. | Apparatus for detecting tumor cells |
| US20130157347A1 (en) * | 2011-07-07 | 2013-06-20 | Veridex, Llc | Method of Analyzing Cardiovascular Disorders and Uses Thereof |
| US9511152B2 (en) * | 2012-04-05 | 2016-12-06 | The Board Of Regents Of The University Of Texas System | Multicolored pH-activatable fluorescence nanoplatform |
| CN104914078A (zh) * | 2015-04-16 | 2015-09-16 | 南京昊控软件技术有限公司 | 一种大范围多点流体浓度测量系统 |
| SG11201807053TA (en) * | 2016-03-07 | 2018-09-27 | X Zell Inc | Compositions and methods for identifying rare cells |
| FR3051556A1 (fr) * | 2016-05-18 | 2017-11-24 | Biocytex | Utilisation de marqueurs fluorescents pour la detection de cible biologique dans un echantillon |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5034613A (en) * | 1989-11-14 | 1991-07-23 | Cornell Research Foundation, Inc. | Two-photon laser microscopy |
| US5127730A (en) * | 1990-08-10 | 1992-07-07 | Regents Of The University Of Minnesota | Multi-color laser scanning confocal imaging system |
| US5698397A (en) * | 1995-06-07 | 1997-12-16 | Sri International | Up-converting reporters for biological and other assays using laser excitation techniques |
| JP3343276B2 (ja) * | 1993-04-15 | 2002-11-11 | 興和株式会社 | レーザー走査型光学顕微鏡 |
| US5479252A (en) * | 1993-06-17 | 1995-12-26 | Ultrapointe Corporation | Laser imaging system for inspection and analysis of sub-micron particles |
-
2003
- 2003-05-05 JP JP2004501911A patent/JP2005524833A/ja active Pending
- 2003-05-05 WO PCT/US2003/013842 patent/WO2003093795A2/en not_active Application Discontinuation
- 2003-05-05 CA CA002484730A patent/CA2484730A1/en not_active Abandoned
- 2003-05-05 EP EP03728685A patent/EP1501917A4/de not_active Withdrawn
- 2003-05-05 AU AU2003234459A patent/AU2003234459A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| WO2003093795A2 (en) | 2003-11-13 |
| AU2003234459A1 (en) | 2003-11-17 |
| WO2003093795A3 (en) | 2004-10-21 |
| AU2003234459A8 (en) | 2003-11-17 |
| EP1501917A2 (de) | 2005-02-02 |
| JP2005524833A (ja) | 2005-08-18 |
| EP1501917A4 (de) | 2006-04-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20060094109A1 (en) | Device and method for analytical cell imaging | |
| CA2484730A1 (en) | Device and method for analytical cell imaging | |
| US7282180B2 (en) | Devices and methods to image objects | |
| AU2008249152B2 (en) | Devices and methods to image objects | |
| US7764821B2 (en) | Methods and algorithms for cell enumeration in a low-cost cytometer | |
| US8189899B2 (en) | Methods and algorithms for cell enumeration in a low-cost cytometer | |
| US7277569B2 (en) | Apparatus and method for detecting and locating rare cells | |
| US5547849A (en) | Apparatus and method for volumetric capillary cytometry | |
| US8128890B2 (en) | Methods and algorithms for cell enumeration in a low-cost cytometer | |
| US20090029378A1 (en) | High sensitivity multiparameter method for rare event analysis in a biological sample | |
| JP2001516050A (ja) | 光散乱材料で標識した試料をイメージングするための装置および方法 | |
| US9933415B2 (en) | Internal focus reference beads for imaging cytometry | |
| WO2014079802A2 (en) | Laser ablation inductively-coupled plasma mass spectral tissue diagnostics | |
| EP1656545B1 (de) | Vorrichtungen und verfahren zur abbildung von objekten mittels "time delay integration" | |
| US20200379227A1 (en) | Method For Analyzing Fluorescent Particles in an Immunoassay | |
| Kagan et al. | CellTracks: Cell analysis system for rare cell detection | |
| US20120202189A1 (en) | Rapid, semi-automated method to detect respiratory virus infected cells in direct specimens | |
| AU2002245215A1 (en) | Devices and methods to image objects | |
| Hsieh et al. | Location and biomarker characterization of circulating tumor cells | |
| Verrant et al. | i, United States Patent (10) Patent No.: US 7,764,821 B2 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Discontinued |