CA2480642C - Lambda integrase mediated recombination in plants - Google Patents

Lambda integrase mediated recombination in plants Download PDF

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Publication number
CA2480642C
CA2480642C CA2480642A CA2480642A CA2480642C CA 2480642 C CA2480642 C CA 2480642C CA 2480642 A CA2480642 A CA 2480642A CA 2480642 A CA2480642 A CA 2480642A CA 2480642 C CA2480642 C CA 2480642C
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CA
Canada
Prior art keywords
integrase
recognition site
int
sequence
plant cell
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Expired - Fee Related
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CA2480642A
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English (en)
French (fr)
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CA2480642A1 (en
Inventor
Janet Louise Suttie
Mary-Dell Chilton
Qiudeng Que
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Syngenta Participations AG
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Syngenta Participations AG
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Publication of CA2480642A1 publication Critical patent/CA2480642A1/en
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Publication of CA2480642C publication Critical patent/CA2480642C/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases RNAses, DNAses
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213Targeted insertion of genes into the plant genome by homologous recombination

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2480642A 2002-03-29 2003-03-28 Lambda integrase mediated recombination in plants Expired - Fee Related CA2480642C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US36904102P 2002-03-29 2002-03-29
US60/369,041 2002-03-29
US42551202P 2002-11-12 2002-11-12
US60/425,512 2002-11-12
PCT/US2003/010124 WO2003083045A2 (en) 2002-03-29 2003-03-28 Lambda integrase mediated recombination in plants

Publications (2)

Publication Number Publication Date
CA2480642A1 CA2480642A1 (en) 2003-10-09
CA2480642C true CA2480642C (en) 2012-10-16

Family

ID=28678262

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2480642A Expired - Fee Related CA2480642C (en) 2002-03-29 2003-03-28 Lambda integrase mediated recombination in plants

Country Status (6)

Country Link
US (2) US7351877B2 (de)
EP (1) EP1572914B1 (de)
JP (1) JP2005521400A (de)
AU (2) AU2003220642C1 (de)
CA (1) CA2480642C (de)
WO (1) WO2003083045A2 (de)

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US7164056B2 (en) * 2002-05-03 2007-01-16 Pioneer Hi-Bred International, Inc. Gene targeting using replicating DNA molecules
US7491539B2 (en) * 2002-12-05 2009-02-17 Boehringer Ingelheim Pharma Kg Sequence specific DNA recombination in eukaryotic cells
TW200502390A (en) * 2002-11-28 2005-01-16 Peter Droege Sequence specific DNA recombination in eukaryotic cells
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WO2007011733A2 (en) 2005-07-18 2007-01-25 Pioneer Hi-Bred International, Inc. Modified frt recombination sites and methods of use
US20070143881A1 (en) * 2005-12-16 2007-06-21 Pioneer Hi-Bred International, Inc. Methods and Compositions for Improving the Efficiency of Site-Specific Polynucleotide Exchange
CA2654415A1 (en) * 2006-05-22 2007-11-29 Hiprocell, Llc. Protein production using eukaryotic cell lines
CN101548011B (zh) 2006-06-03 2018-04-27 先正达参股股份有限公司 玉米事件mir162
WO2009006297A2 (en) * 2007-06-29 2009-01-08 Pioneer Hi-Bred International, Inc. Methods for altering the genome of a monocot plant cell
WO2009114321A2 (en) * 2008-03-11 2009-09-17 Precision Biosciencs, Inc. Rationally-designed meganucleases for maize genome engineering
JP2011519558A (ja) * 2008-04-28 2011-07-14 プレシジョン バイオサイエンシズ,インク. 合理的に設計されたdna結合タンパク質とエフェクタードメインとの融合分子
UA119135C2 (uk) 2012-09-07 2019-05-10 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб отримання трансгенної рослини
EP3113630B1 (de) 2014-03-06 2019-12-11 The Research Institute at Nationwide Children's Hospital Probiotische formulierungen und verfahren zur verwendung
US10624934B2 (en) * 2014-03-06 2020-04-21 Research Institute At Nationwide Children's Hospital Prebiotic formulations
EP3365027B1 (de) 2015-10-14 2022-03-30 Research Institute at Nationwide Children's Hospital Hu-spezifische antikörper und deren verwendeng zur hemmung von biofilm
CN114207131A (zh) * 2019-08-05 2022-03-18 孟山都技术公司 用于染色体重排的组合物和方法
CN112852828A (zh) * 2019-11-27 2021-05-28 江苏师范大学 一种植物花粉管液泡荧光标记构建方法与应用

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US5227288A (en) * 1990-10-01 1993-07-13 Blattner Frederick R DNA sequencing vector with reversible insert
UA48104C2 (uk) * 1991-10-04 2002-08-15 Новартіс Аг Фрагмент днк, який містить послідовність,що кодує інсектицидний протеїн, оптимізовану для кукурудзи,фрагмент днк, який забезпечує направлену бажану для серцевини стебла експресію зв'язаного з нею структурного гена в рослині, фрагмент днк, який забезпечує специфічну для пилку експресію зв`язаного з нею структурного гена в рослині, рекомбінантна молекула днк, спосіб одержання оптимізованої для кукурудзи кодуючої послідовності інсектицидного протеїну, спосіб захисту рослин кукурудзи щонайменше від однієї комахи-шкідника
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ATE222289T1 (de) 1995-06-07 2002-08-15 Invitrogen Corp Rekombinatorische klonierung in vitro unter verwendung genmanipulierter rekombinationsorte
US6143557A (en) 1995-06-07 2000-11-07 Life Technologies, Inc. Recombination cloning using engineered recombination sites
US6051409A (en) 1995-09-25 2000-04-18 Novartis Finance Corporation Method for achieving integration of exogenous DNA delivered by non-biological means to plant cells
JP4303418B2 (ja) 1997-10-24 2009-07-29 ライフ テクノロジーズ コーポレーション 組換え部位を有する核酸を使用する組換えクローニング
ATE454459T1 (de) 1997-11-18 2010-01-15 Pioneer Hi Bred Int Mobilisierung eines viralen genoms aus t-dna durch ortsspezifische rekombinationssysteme
ES2245487T3 (es) 1997-11-18 2006-01-01 Pioneer Hi-Bred International, Inc. Composiciones y metodos para la modificacion genetica de plantas.
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Also Published As

Publication number Publication date
EP1572914A4 (de) 2007-08-08
CA2480642A1 (en) 2003-10-09
AU2008229766A1 (en) 2008-10-30
AU2003220642A1 (en) 2003-10-13
JP2005521400A (ja) 2005-07-21
AU2003220642B2 (en) 2008-07-03
EP1572914A2 (de) 2005-09-14
US20030226164A1 (en) 2003-12-04
US7351877B2 (en) 2008-04-01
US20060130179A1 (en) 2006-06-15
WO2003083045A2 (en) 2003-10-09
WO2003083045A3 (en) 2006-07-20
AU2003220642C1 (en) 2009-01-29
AU2008229766B2 (en) 2011-10-20
EP1572914B1 (de) 2012-06-06

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