CA2469477A1 - Phenotypic screen of chimeric proteins - Google Patents

Phenotypic screen of chimeric proteins Download PDF

Info

Publication number
CA2469477A1
CA2469477A1 CA002469477A CA2469477A CA2469477A1 CA 2469477 A1 CA2469477 A1 CA 2469477A1 CA 002469477 A CA002469477 A CA 002469477A CA 2469477 A CA2469477 A CA 2469477A CA 2469477 A1 CA2469477 A1 CA 2469477A1
Authority
CA
Canada
Prior art keywords
cell
nucleic acid
protein
transcription factor
zinc finger
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002469477A
Other languages
English (en)
French (fr)
Inventor
Jin-Soo Kim
Kyung-Soon Park
Dong-Ki Lee
Wongi Seol
Horim Lee
Seong-Il Lee
Hyo-Young Yang
Yangsoon Lee
Young-Soon Jang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Toolgen Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2469477A1 publication Critical patent/CA2469477A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10Cells modified by introduction of foreign genetic material
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1079Screening libraries by altering the phenotype or phenotypic trait of the host
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04Plant cells or tissues
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00Genetically modified cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00Genetically modified cells
    • C12N2510/02Cells for production

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA002469477A 2001-12-07 2002-12-07 Phenotypic screen of chimeric proteins Abandoned CA2469477A1 (en)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US33844101P 2001-12-07 2001-12-07
US60/338,441 2001-12-07
US37605302P 2002-04-26 2002-04-26
US60/376,053 2002-04-26
US40090402P 2002-08-02 2002-08-02
US60/400,904 2002-08-02
US40108902P 2002-08-05 2002-08-05
US60/401,089 2002-08-05
PCT/KR2002/002309 WO2003048345A1 (en) 2001-12-07 2002-12-07 Phenotypic screen of chimeric proteins

Publications (1)

Publication Number Publication Date
CA2469477A1 true CA2469477A1 (en) 2003-06-12

Family

ID=27502584

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002469477A Abandoned CA2469477A1 (en) 2001-12-07 2002-12-07 Phenotypic screen of chimeric proteins

Country Status (8)

Country Link
US (2) US20030194727A1 (enExample)
EP (1) EP1451297A4 (enExample)
JP (1) JP2005511049A (enExample)
KR (1) KR20040065235A (enExample)
CN (1) CN100463962C (enExample)
AU (1) AU2002365796A1 (enExample)
CA (1) CA2469477A1 (enExample)
WO (1) WO2003048345A1 (enExample)

Families Citing this family (84)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1417344B1 (en) * 2001-08-17 2011-06-15 Toolgen, Inc. Zinc finger domain libraries
EP1451297A4 (en) 2001-12-07 2006-06-28 Toolgen Inc PHENOTYPIC SCANNING OF CHIMERIC PROTEINS
US20030180777A1 (en) * 2002-03-12 2003-09-25 Victor Bartsevich Rapid identification of transcriptional regulatory domains
US20050032186A1 (en) * 2002-12-09 2005-02-10 Kim Jin-Soo Regulatory zinc finger proteins
WO2005005662A2 (en) * 2003-07-08 2005-01-20 Axiogenesis Ag Secreted proteins as markers for cell differentiation
WO2005061705A1 (en) * 2003-12-23 2005-07-07 Toolgen, Inc. Regulation of prokaryotic gene expression with zinc finger proteins
BRPI0514343A (pt) * 2004-08-13 2008-06-10 Anormed Inc combinações de quimiocinas para mobilizar células progenitoras/tronco
US7855279B2 (en) 2005-09-27 2010-12-21 Amunix Operating, Inc. Unstructured recombinant polymers and uses thereof
US20090099031A1 (en) * 2005-09-27 2009-04-16 Stemmer Willem P Genetic package and uses thereof
US20070212703A1 (en) * 2005-09-27 2007-09-13 Stemmer Willem P Proteinaceous pharmaceuticals and uses thereof
US7846445B2 (en) * 2005-09-27 2010-12-07 Amunix Operating, Inc. Methods for production of unstructured recombinant polymers and uses thereof
WO2007058833A2 (en) 2005-11-10 2007-05-24 Pioneer Hi-Bred International, Inc. Dof (dna binding with one finger) sequences and methods of use
AR060523A1 (es) 2006-04-19 2008-06-25 Pioneer Hi Bred Int Moleculas de polinucleotidos aislados que corresponden a alelos mutantes y tipo salvaje del gen de maiz d9 y metodos para usarlas
WO2007137114A2 (en) 2006-05-17 2007-11-29 Pioneer Hi-Bred International, Inc. Artificial plant minichromosomes
EP2032149A4 (en) * 2006-06-13 2010-09-01 Fmc Biopolymer As METHOD AND SYSTEMS FOR THE USE OF BIOPOLYMER-BASED BEADS AND HYDROGELS
BRPI0715393A2 (pt) * 2006-08-07 2013-06-25 Genzyme Corp terapia de combinaÇço
KR100812110B1 (ko) 2006-10-24 2008-03-12 한국과학기술원 징크 핑거 단백질과 원핵 생물의 전사 인자를 포함하는인공 전사 인자의 제조 및 이의 이용
US7642436B2 (en) 2007-04-13 2010-01-05 Ball Horticultural Company Petunia mutant allele
CA2687211A1 (en) 2007-05-25 2008-12-04 Cropdesign N.V. Yield enhancement in plants by modulation of maize alfins
EP2164863A4 (en) 2007-06-15 2010-07-28 Univ Arkansas METHOD FOR THE ADMINISTRATION OF MOLECULES IN CELLS USING A RICIN SUB-UNIT AND RELATED COMPOSITIONS THEREOF
BRPI0815416A2 (pt) * 2007-08-15 2014-10-21 Amunix Inc Composições e métodos para modificar propriedades de polipeptídeos biologicamente ativos
US8829282B2 (en) 2008-05-14 2014-09-09 Monsanto Technology, Llc Plants and seeds of spring canola variety SCV425044
US7964774B2 (en) 2008-05-14 2011-06-21 Monsanto Technology Llc Plants and seeds of spring canola variety SCV384196
US7935870B2 (en) 2008-05-14 2011-05-03 Monsanto Technology Llc Plants and seeds of spring canola variety SCV354718
US7947877B2 (en) 2008-05-14 2011-05-24 Monosanto Technology LLC Plants and seeds of spring canola variety SCV328921
AU2009329893A1 (en) * 2008-12-23 2011-06-30 Vivoscript, Inc. Compositions and methods for re-programming cells without genetic modification
EP2387567B1 (en) 2009-01-14 2014-06-11 The Salk Institute for Biological Studies Compounds that protect against amyloid diseases
WO2010091122A1 (en) * 2009-02-03 2010-08-12 Amunix, Inc. Extended recombinant polypeptides and compositions comprising same
WO2010118477A1 (en) 2009-04-17 2010-10-21 Molecular Plant Breeding Nominees Ltd Plant promoter operable in endosperm and uses thereof
CN102439032B (zh) 2009-05-04 2015-07-22 先锋国际良种公司 通过调节ap2转录因子增加植物中的产量
US8071848B2 (en) 2009-06-17 2011-12-06 Monsanto Technology Llc Plants and seeds of spring canola variety SCV218328
BR112012004094A2 (pt) 2009-08-24 2016-03-08 Amunix Operating Inc composições de fator vii de coagulação e métodos para fazer e usar as mesmas
US8440891B2 (en) 2009-09-22 2013-05-14 Board of Trustees of the University of Akransas, N.A. Rice cultivar CL 142-AR
US8440892B2 (en) 2009-10-15 2013-05-14 Board Of Trustees Of The University Of Arkansas, N.A. Rice cultivar CL 181-AR
EP2529018B1 (en) 2009-12-30 2016-06-22 Pioneer Hi-Bred International, Inc. Methods and compositions for the introduction and regulated expression of genes in plants
AU2010339481B2 (en) 2009-12-30 2016-02-04 Pioneer Hi-Bred International, Inc. Methods and compositions for targeted polynucleotide modification
US20130189780A1 (en) * 2009-12-31 2013-07-25 Fate Therapeutics, Inc. Reprogramming compositions
US8148611B2 (en) 2010-02-26 2012-04-03 Monsanto Technology Llc Plants and seeds of spring canola variety SCV453784
US8143488B2 (en) 2010-02-26 2012-03-27 Monsanto Technoloy LLC Plants and seeds of spring canola variety SCV470336
US8138394B2 (en) 2010-02-26 2012-03-20 Monsanto Technology Llc Plants and seeds of spring canola variety SCV431158
US8581048B2 (en) 2010-03-09 2013-11-12 Monsanto Technology, Llc Plants and seeds of spring canola variety SCV119103
US8153865B2 (en) 2010-03-11 2012-04-10 Monsanto Technology Llc Plants and seeds of spring canola variety SCV152154
BR112012028052A2 (pt) 2010-05-06 2019-09-24 Du Pont polipeptídeo, polinucleotídeo, cassete de expressão,planta e método de melhoramento de tolerância a seca em uma planta.
US8513487B2 (en) 2011-04-07 2013-08-20 Zenon LISIECZKO Plants and seeds of spring canola variety ND-662c
US8513494B2 (en) 2011-04-08 2013-08-20 Chunren Wu Plants and seeds of spring canola variety SCV695971
US8507761B2 (en) 2011-05-05 2013-08-13 Teresa Huskowska Plants and seeds of spring canola variety SCV372145
US8513495B2 (en) 2011-05-10 2013-08-20 Dale Burns Plants and seeds of spring canola variety SCV291489
US8785729B2 (en) 2011-08-09 2014-07-22 Nunhems, B.V. Lettuce variety redglace
US8754293B2 (en) 2011-09-09 2014-06-17 Nunhems B.V. Lettuce variety intred
WO2013096810A1 (en) 2011-12-21 2013-06-27 The Curators Of The University Of Missouri Soybean variety s05-11482
WO2013096818A1 (en) 2011-12-21 2013-06-27 The Curators Of The University Of Missouri Soybean variety s05-11268
US9380756B2 (en) 2012-01-04 2016-07-05 Nunhems B.V. Lettuce variety multigreen 50
LT2822577T (lt) 2012-02-15 2019-03-25 Bioverativ Therapeutics Inc. Rekombinantiniai faktoriaus viii baltymai
HRP20221531T1 (hr) 2012-02-15 2023-02-17 Bioverativ Therapeutics Inc. Pripravci faktora viii i postupci dobivanja i korištenja istih
US8859857B2 (en) 2012-04-26 2014-10-14 Monsanto Technology Llc Plants and seeds of spring canola variety SCV259778
US8835720B2 (en) 2012-04-26 2014-09-16 Monsanto Technology Llc Plants and seeds of spring canola variety SCV967592
US8878009B2 (en) 2012-04-26 2014-11-04 Monsanto Technology, LLP Plants and seeds of spring canola variety SCV318181
US8802935B2 (en) 2012-04-26 2014-08-12 Monsanto Technology Llc Plants and seeds of spring canola variety SCV942568
CN102816214B (zh) * 2012-07-27 2014-08-13 中国人民解放军军事医学科学院生物工程研究所 乙型肝炎病毒HBx基因的人工转录因子及其应用
CN102994437B (zh) * 2012-11-06 2014-04-02 大连理工大学 高产木质纤维素降解酶的链霉菌人工锌指蛋白突变菌株及其获得方法
EP3033097B1 (en) 2013-08-14 2021-03-10 Bioverativ Therapeutics Inc. Factor viii-xten fusions and uses thereof
WO2016007640A1 (en) 2014-07-10 2016-01-14 Benson Hill Biosystems, Inc. Compositions and methods for increasing plant growth and yield
MX2018001497A (es) 2015-08-03 2018-05-15 Bioverativ Therapeutics Inc Proteinas de fusion de factor ix y metodos para producirlas y usarlas.
IL293963A (en) 2015-09-01 2022-08-01 Univ California Modular polypeptide libraries and methods of making and using same
US10947554B2 (en) 2015-10-23 2021-03-16 Donald Danforth Plant Science Center Expression of dicarboxylate transporter from setaria italica in transgenic plants to increase yield
EP3372985B1 (en) 2015-10-28 2025-06-25 The University Of Tokyo Analysis device
US11330776B2 (en) 2015-10-30 2022-05-17 Pioneer Hi-Bred International, Inc. Methods and compositions for rapid plant transformation
WO2017078836A1 (en) 2015-11-06 2017-05-11 Pioneer Hi-Bred International, Inc. Methods and compositions of improved plant transformation
WO2018102743A1 (en) 2016-12-02 2018-06-07 Bioverativ Therapeutics Inc. Methods of treating hemophilic arthropathy using chimeric clotting factors
AU2019234474B2 (en) 2018-03-12 2025-05-15 Pioneer Hi-Bred International, Inc. Methods for plant transformation
RS66972B1 (sr) 2018-05-18 2025-07-31 Bioverativ Therapeutics Inc Metode lečenja hemofilije a
WO2019241443A1 (en) 2018-06-13 2019-12-19 Thinkcyte Inc. Methods and systems for cytometry
US20210277409A1 (en) 2018-06-28 2021-09-09 Pioneer Hi-Bred International, Inc. Methods for selecting transformed plants
CN113226333A (zh) 2018-10-02 2021-08-06 桑格摩生物治疗股份有限公司 用于调控Tau蛋白的方法和组合物
WO2020081819A1 (en) * 2018-10-18 2020-04-23 Thinkcyte Inc. Methods and systems for target screening
JP2022512817A (ja) 2018-10-31 2022-02-07 パイオニア ハイ-ブレッド インターナショナル, インコーポレイテッド オクロバクテリウム(Ochrobactrum)媒介植物形質転換のための組成物及び方法
EP4083603A4 (en) 2019-12-27 2024-04-03 ThinkCyte, Inc. FLOW CYTOMETER PERFORMANCE EVALUATION METHODS AND STANDARD PARTICLE SUSPENSION
WO2021138560A2 (en) 2020-01-02 2021-07-08 The Trustees Of Columbia University In The City Of New York Programmable and portable crispr-cas transcriptional activation in bacteria
WO2021200911A1 (ja) 2020-04-01 2021-10-07 シンクサイト株式会社 フローサイトメーター
WO2021200960A1 (ja) 2020-04-01 2021-10-07 シンクサイト株式会社 観察装置
CN113077849B (zh) * 2021-03-16 2023-03-31 华南农业大学 一种大肠杆菌β-内酰胺类获得性耐药表型预测复合方法
CN113643758B (zh) * 2021-09-22 2023-04-07 华南农业大学 面向肠杆科细菌获得抗β-内酰胺类耐药性基因的预测方法
CN115873900A (zh) * 2021-12-28 2023-03-31 武汉爱博泰克生物科技有限公司 胞外表达人源Ly6d蛋白的重组表达质粒、重组细胞及构建方法
CN114360652B (zh) * 2022-01-28 2023-04-28 深圳太力生物技术有限责任公司 细胞株相似性评价方法及相似细胞株培养基配方推荐方法

Family Cites Families (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5763209A (en) * 1988-09-26 1998-06-09 Arch Development Corporation Methods and materials relating to the functional domains of DNA binding proteins
US6107059A (en) * 1992-04-29 2000-08-22 Affymax Technologies N.V. Peptide library and screening method
US6242568B1 (en) * 1994-01-18 2001-06-05 The Scripps Research Institute Zinc finger protein derivatives and methods therefor
US6140466A (en) * 1994-01-18 2000-10-31 The Scripps Research Institute Zinc finger protein derivatives and methods therefor
US5882941A (en) * 1994-05-04 1999-03-16 Massachusette Institute Of Technology Programmable genotoxic agents and uses therefor
EP0781331B1 (en) * 1994-08-20 2008-09-03 Gendaq Limited Improvements in or relating to binding proteins for recognition of dna
US6326166B1 (en) * 1995-12-29 2001-12-04 Massachusetts Institute Of Technology Chimeric DNA-binding proteins
US5789538A (en) * 1995-02-03 1998-08-04 Massachusetts Institute Of Technology Zinc finger proteins with high affinity new DNA binding specificities
US5866941A (en) * 1995-02-23 1999-02-02 Silicon Systems, Inc. Ultra thin, leadless and molded surface mount integrated circuit package
GB9710809D0 (en) * 1997-05-23 1997-07-23 Medical Res Council Nucleic acid binding proteins
US20030166107A1 (en) * 1997-09-18 2003-09-04 Genentech, Inc. Secreted and transmembrane polypeptides and nucleic acids encoding the same
WO1999045132A1 (en) * 1998-03-02 1999-09-10 Massachusetts Institute Of Technology Poly zinc finger proteins with improved linkers
US7013219B2 (en) * 1999-01-12 2006-03-14 Sangamo Biosciences, Inc. Regulation of endogenous gene expression in cells using zinc finger proteins
US6534261B1 (en) * 1999-01-12 2003-03-18 Sangamo Biosciences, Inc. Regulation of endogenous gene expression in cells using zinc finger proteins
US6453242B1 (en) * 1999-01-12 2002-09-17 Sangamo Biosciences, Inc. Selection of sites for targeting by zinc finger proteins and methods of designing zinc finger proteins to bind to preselected sites
US6599692B1 (en) * 1999-09-14 2003-07-29 Sangamo Bioscience, Inc. Functional genomics using zinc finger proteins
US20020164575A1 (en) * 1999-09-14 2002-11-07 Sangamo Biosciences, Inc., A Delaware Corporation Gene identification
AU776576B2 (en) * 1999-12-06 2004-09-16 Sangamo Biosciences, Inc. Methods of using randomized libraries of zinc finger proteins for the identification of gene function
ATE355368T1 (de) * 2000-01-24 2006-03-15 Gendaq Ltd Nucleinsäure bindende polypeptide gekennzeichnet durch flexible linker verbundene nucleinsäuredomäne
JP5047437B2 (ja) * 2000-02-08 2012-10-10 サンガモ バイオサイエンシーズ, インコーポレイテッド 薬物の発見のための細胞
US20020061512A1 (en) * 2000-02-18 2002-05-23 Kim Jin-Soo Zinc finger domains and methods of identifying same
AU2001257331A1 (en) * 2000-04-28 2001-11-12 Sangamo Biosciences, Inc. Methods for designing exogenous regulatory molecules
WO2001088197A2 (en) * 2000-05-16 2001-11-22 Massachusetts Institute Of Technology Methods and compositions for interaction trap assays
US6492117B1 (en) * 2000-07-12 2002-12-10 Gendaq Limited Zinc finger polypeptides capable of binding DNA quadruplexes
US7067317B2 (en) * 2000-12-07 2006-06-27 Sangamo Biosciences, Inc. Regulation of angiogenesis with zinc finger proteins
US7026462B2 (en) * 2000-12-07 2006-04-11 Sangamo Biosciences, Inc. Regulation of angiogenesis with zinc finger proteins
EP1417344B1 (en) * 2001-08-17 2011-06-15 Toolgen, Inc. Zinc finger domain libraries
US20040214766A1 (en) * 2001-10-01 2004-10-28 Kari Alitalo VEGF-C or VEGF-D materials and methods for treatment of neuropathologies
EP1451297A4 (en) * 2001-12-07 2006-06-28 Toolgen Inc PHENOTYPIC SCANNING OF CHIMERIC PROTEINS

Also Published As

Publication number Publication date
CN1610737A (zh) 2005-04-27
AU2002365796A1 (en) 2003-06-17
WO2003048345A1 (en) 2003-06-12
CN100463962C (zh) 2009-02-25
US7514257B2 (en) 2009-04-07
EP1451297A1 (en) 2004-09-01
JP2005511049A (ja) 2005-04-28
KR20040065235A (ko) 2004-07-21
EP1451297A4 (en) 2006-06-28
US20030194727A1 (en) 2003-10-16
US20040209277A1 (en) 2004-10-21

Similar Documents

Publication Publication Date Title
US7514257B2 (en) Zinc finger transcription factor differentiation proteins
Strunnikov et al. SMC2, a Saccharomyces cerevisiae gene essential for chromosome segregation and condensation, defines a subgroup within the SMC family.
JP4156840B2 (ja) ジンクフィンガードメイン及びその同定方法
CA2290720C (en) Nucleic acid binding polypeptide library
Quadbeck‐Seeger et al. A protein with similarity to the human retinoblastoma binding protein 2 acts specifically as a repressor for genes regulated by the b mating type locus in Ustilago maydis
Freire Translation initiation factor (iso) 4E interacts with BTF3, the β subunit of the nascent polypeptide-associated complex
JP2007075107A (ja) 逆ツーハイブリッドシステム
WO1998053059A1 (en) Nucleic acid binding proteins
US20090176653A1 (en) Zinc finger domain libraries
AU2002324352A1 (en) Zinc finger domain libraries
CN113711046A (zh) 用于揭示与Tau聚集相关的基因脆弱性的CRISPR/Cas脱落筛选平台
Nagata et al. Tobacco BY-2 cells
CA2508631A1 (en) Regulatory zinc finger proteins
Jiang et al. Construction of comprehensive dosage-matching core histone mutant libraries for Saccharomyces cerevisiae
US20070042378A1 (en) Regulation of prokaryotic gene expression with zinc finger proteins
US20040259258A1 (en) Regulation of prokaryotic gene expression with zinc finger proteins
Sanchez Garcia et al. Functional mapping of the fission yeast DNA polymerase δ B-subunit Cdc1 by site-directed and random pentapeptide insertion mutagenesis
WO2004022575A2 (en) Bioinformatics analysis of cellular effects of artificial transcription factors
Fiore et al. Interaction-Based Cloning of Novel Proteins
Park Screening new cytokinesis genes and investigation of regulation of Hof1 in cytokinesis
Převorovský CSL proteins of Schizosaccharomyces pombe
Strunnikov et al. and condensation, defines a subgroup within the SMC family
Ma Studies on the Mechanisms of Homolog Pairing and Sister Chromatid Cohesion During Drosophila Male Meiosis

Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued