CA2465594C - Compositions de microparticules marquees et procedes associes - Google Patents

Compositions de microparticules marquees et procedes associes Download PDF

Info

Publication number
CA2465594C
CA2465594C CA2465594A CA2465594A CA2465594C CA 2465594 C CA2465594 C CA 2465594C CA 2465594 A CA2465594 A CA 2465594A CA 2465594 A CA2465594 A CA 2465594A CA 2465594 C CA2465594 C CA 2465594C
Authority
CA
Canada
Prior art keywords
molecular tags
molecular
binding
composition
binding moiety
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2465594A
Other languages
English (en)
Other versions
CA2465594A1 (fr
Inventor
Peter Dehlinger
Sharat Singh
Hrair Kirakossian
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Monogram Biosciences Inc
Original Assignee
Monogram Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monogram Biosciences Inc filed Critical Monogram Biosciences Inc
Publication of CA2465594A1 publication Critical patent/CA2465594A1/fr
Application granted granted Critical
Publication of CA2465594C publication Critical patent/CA2465594C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D495/00—Heterocyclic compounds containing in the condensed system at least one hetero ring having sulfur atoms as the only ring hetero atoms
    • C07D495/02—Heterocyclic compounds containing in the condensed system at least one hetero ring having sulfur atoms as the only ring hetero atoms in which the condensed system contains two hetero rings
    • C07D495/04—Ortho-condensed systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D405/00—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom
    • C07D405/02—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings
    • C07D405/12—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings linked by a chain containing hetero atoms as chain links
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D493/00—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system
    • C07D493/02—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system in which the condensed system contains two hetero rings
    • C07D493/10—Spiro-condensed systems
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Food Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

L'invention concerne des compositions et des procédés de détection de nombreux mélanges cibles à analyser dans un échantillon au moyen de microparticules présentant des marqueurs moléculaires qui sont fixés par des liaisons pouvant être coupées. De manière générale, un mélange d'analyse est formé et comprend un échantillon et un réactif comprenant de nombreuses microparticules dans un état permettant à des complexes stables de se former entre les fractions de liaisons sur lesdites surfaces des microparticules et lesdits mélanges à analyser. Selon un aspect de l'invention, une seconde composition de liaison est ajoutée de sorte que des complexes se forment parmi les fractions de liaison liées aux microparticules, lesdits mélanges à analyser, et les secondes fractions de liaison de la seconde composition de liaison. Lesdites secondes fractions présentent des fractions induisant le clivage fixées qui provoquent le clivage desdites liaisons et la libération des marqueurs moléculaires à la suite de l'activation. Lesdits marqueurs moléculaires libérés sont séparés et la présence et/ou la quantité des mélanges cibles à analyser sont déterminées sur la base de l'analyse des marqueurs moléculaires libérés et séparés.
CA2465594A 2001-11-09 2002-11-08 Compositions de microparticules marquees et procedes associes Expired - Fee Related CA2465594C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US33776801P 2001-11-09 2001-11-09
US60/337,768 2001-11-09
PCT/US2002/035907 WO2003042699A1 (fr) 2001-11-09 2002-11-08 Compositions de microparticules marquees et procedes associes

Publications (2)

Publication Number Publication Date
CA2465594A1 CA2465594A1 (fr) 2003-05-22
CA2465594C true CA2465594C (fr) 2012-02-07

Family

ID=23321917

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2465594A Expired - Fee Related CA2465594C (fr) 2001-11-09 2002-11-08 Compositions de microparticules marquees et procedes associes

Country Status (4)

Country Link
EP (1) EP1456657A4 (fr)
JP (1) JP2005509859A (fr)
CA (1) CA2465594C (fr)
WO (1) WO2003042699A1 (fr)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040229294A1 (en) 2002-05-21 2004-11-18 Po-Ying Chan-Hui ErbB surface receptor complexes as biomarkers
EP1494028A1 (fr) * 2003-07-03 2005-01-05 Labsoft Diagnostics AG Séparation immuno-magnétique des cellulles cibles spécifiques
US20050266578A1 (en) * 2004-05-06 2005-12-01 Gruenke Larry D Methods and systems for detection of macrolides
EP1747295B1 (fr) * 2004-05-12 2012-01-11 Nanosphere, Inc. Detection d'analytes cibles fondee sur des codes a barres biologiques
WO2007061098A1 (fr) * 2005-11-25 2007-05-31 Japan Science And Technology Agency Analyseur et procede d’analyse
EP2235536A4 (fr) 2007-12-20 2011-05-04 Lab Corp America Holdings Procédés de diagnostic du her-2
US8622987B2 (en) 2008-06-04 2014-01-07 The University Of Chicago Chemistrode, a plug-based microfluidic device and method for stimulation and sampling with high temporal, spatial, and chemical resolution
US8349574B2 (en) 2009-01-15 2013-01-08 Laboratory Corporation Of America Holdings Methods of determining patient response by measurement of Her-3
CA2764386C (fr) 2008-12-01 2018-05-01 Laboratory Corporation Of America Holdings Anticorps p35-her2 et leurs utilisations
CN108780089B (zh) 2016-03-15 2020-09-08 美国控股实验室公司 评估细胞间的蛋白质相互作用的方法
CN107764906A (zh) * 2016-08-22 2018-03-06 洛阳惠中兽药有限公司 一种加米霉素的含量检测方法
CN110823872A (zh) * 2018-08-13 2020-02-21 博阳生物科技（上海）有限公司 一种用于化学发光分析的微球组合物及其应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5310687A (en) * 1984-10-31 1994-05-10 Igen, Inc. Luminescent metal chelate labels and means for detection
US5935779A (en) * 1988-11-03 1999-08-10 Igen International Inc. Methods for improved particle electrochemiluminescence assay
US5326692B1 (en) * 1992-05-13 1996-04-30 Molecular Probes Inc Fluorescent microparticles with controllable enhanced stokes shift
US5846703A (en) * 1990-05-15 1998-12-08 Diatron Corporation Fluorescence immunoassays using fluorescent dyes free of aggregation and serum binding
US5736410A (en) * 1992-09-14 1998-04-07 Sri International Up-converting reporters for biological and other assays using laser excitation techniques
WO1996033411A1 (fr) * 1995-04-18 1996-10-24 Igen, Inc. L'electrochimioluminescence de chelates de metaux de terres rares
JP2002504689A (ja) * 1998-02-18 2002-02-12 デイド・ベーリング・インコーポレイテッド 複数の検体の検出において使用するための化学ルミネセンス組成物
US6322980B1 (en) * 1999-04-30 2001-11-27 Aclara Biosciences, Inc. Single nucleotide detection using degradation of a fluorescent sequence
WO2002095356A2 (fr) * 2001-05-21 2002-11-28 Aclara Biosciences, Inc. Procedes et compositions pour l'analyse de proteines
US7041459B2 (en) * 2001-05-21 2006-05-09 Monogram Biosciences, Inc. Analyzing phosphorylated proteins

Also Published As

Publication number Publication date
WO2003042699A1 (fr) 2003-05-22
CA2465594A1 (fr) 2003-05-22
JP2005509859A (ja) 2005-04-14
EP1456657A1 (fr) 2004-09-15
EP1456657A4 (fr) 2008-03-12

Similar Documents

Publication Publication Date Title
US7160735B2 (en) Tagged microparticle compositions and methods
US20030207300A1 (en) Multiplex analytical platform using molecular tags
US9939447B2 (en) Methods and compositions for analyzing proteins
US7217531B2 (en) Multiplex analysis using membrane-bound sensitizers
US20050048553A1 (en) Multiplexed analysis by chromatographic separation of molecular tags
US7041459B2 (en) Analyzing phosphorylated proteins
CA2465594C (fr) Compositions de microparticules marquees et procedes associes
WO2003042398A2 (fr) Analyse multiplexee par separation chromatographique d'etiquettes moleculaires
US20060127947A1 (en) Receptor deorphanization using tagged molecular libraries
JP2007504482A6 (ja) タグ付加分子ライブラリーを使用した受容体の脱オーファン化
AU2002340431A1 (en) Tagged microparticle compositions and methods
Cassiano In vitro and in cell functional proteomics on natural compounds

Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed

Effective date: 20171108