CA2430475A1 - Methods and cells for detecting modulators of rgs proteins - Google Patents

Methods and cells for detecting modulators of rgs proteins Download PDF

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Publication number
CA2430475A1
CA2430475A1 CA002430475A CA2430475A CA2430475A1 CA 2430475 A1 CA2430475 A1 CA 2430475A1 CA 002430475 A CA002430475 A CA 002430475A CA 2430475 A CA2430475 A CA 2430475A CA 2430475 A1 CA2430475 A1 CA 2430475A1
Authority
CA
Canada
Prior art keywords
terminus
rgs4
cell
rgs
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002430475A
Other languages
English (en)
French (fr)
Inventor
Kathleen H. Young
Jian Cao
David Sheuy
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wyeth LLC
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2430475A1 publication Critical patent/CA2430475A1/en
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA002430475A 2000-12-01 2001-12-03 Methods and cells for detecting modulators of rgs proteins Abandoned CA2430475A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US25014700P 2000-12-01 2000-12-01
US60/250,147 2000-12-01
PCT/US2001/045105 WO2002050104A2 (en) 2000-12-01 2001-12-03 Methods and cells for detecting modulators of rgs proteins

Publications (1)

Publication Number Publication Date
CA2430475A1 true CA2430475A1 (en) 2002-06-27

Family

ID=22946480

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002430475A Abandoned CA2430475A1 (en) 2000-12-01 2001-12-03 Methods and cells for detecting modulators of rgs proteins

Country Status (9)

Country Link
EP (1) EP1339740A2 (zh)
JP (1) JP2004520824A (zh)
KR (1) KR20030081337A (zh)
CN (1) CN1478097A (zh)
AU (1) AU2002243255A1 (zh)
CA (1) CA2430475A1 (zh)
IL (1) IL156199A0 (zh)
MX (1) MXPA03004700A (zh)
WO (1) WO2002050104A2 (zh)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7846671B2 (en) 2002-01-28 2010-12-07 Bristol-Myers Squibb Company Methods of screening for agents that modulate the interaction of RGS and Gαq and urinary incontinence
KR100968640B1 (ko) * 2007-10-06 2010-07-06 재단법인서울대학교산학협력재단 단일세포의 신호분석방법
US10364421B2 (en) * 2015-02-06 2019-07-30 Cargill, Incorporated Modified glucoamylase enzymes and yeast strains having enhanced bioproduct production
CN105126079B (zh) * 2015-09-29 2019-03-01 武汉大学 G蛋白信号转导调节蛋白14（rgs14）在治疗心肌肥厚中的功能及应用
CN105106936B (zh) * 2015-09-29 2018-05-15 武汉大学 G蛋白信号转导调节蛋白10（rgs10）在治疗心肌肥厚中的功能及应用
CA3032736A1 (en) 2016-08-05 2018-02-08 Cargill, Incorporated Leader-modified glucoamylase polypeptides and engineered yeast strains having enhanced bioproduct production

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU733007B2 (en) * 1996-09-24 2001-05-03 Cadus Pharmaceutical Corporation Methods and compositions for identifying receptor effectors
AU3977699A (en) * 1998-05-08 1999-11-29 Cadus Pharmaceutical Corporation Ags proteins and nucleic acid molecules and uses therefor

Also Published As

Publication number Publication date
IL156199A0 (en) 2003-12-23
WO2002050104A9 (en) 2002-12-19
WO2002050104A3 (en) 2003-04-10
CN1478097A (zh) 2004-02-25
WO2002050104A2 (en) 2002-06-27
AU2002243255A1 (en) 2002-07-01
KR20030081337A (ko) 2003-10-17
JP2004520824A (ja) 2004-07-15
MXPA03004700A (es) 2003-08-19
EP1339740A2 (en) 2003-09-03

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Legal Events

Date Code Title Description
FZDE Discontinued