CA2426731A1 - Agent d'extraction a code isotopique instable vis-a-vis de l'acide et son utilisation dans l'analyse spectrometrique de masse quantitative de melanges de proteines - Google Patents
Agent d'extraction a code isotopique instable vis-a-vis de l'acide et son utilisation dans l'analyse spectrometrique de masse quantitative de melanges de proteines Download PDFInfo
- Publication number
- CA2426731A1 CA2426731A1 CA002426731A CA2426731A CA2426731A1 CA 2426731 A1 CA2426731 A1 CA 2426731A1 CA 002426731 A CA002426731 A CA 002426731A CA 2426731 A CA2426731 A CA 2426731A CA 2426731 A1 CA2426731 A1 CA 2426731A1
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- Prior art keywords
- peptides
- reagent
- polymer
- cysteine
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- Abandoned
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6848—Methods of protein analysis involving mass spectrometry
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D405/00—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom
- C07D405/02—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings
- C07D405/12—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings linked by a chain containing hetero atoms as chain links
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/38—Flow patterns
- G01N30/46—Flow patterns using more than one column
- G01N30/461—Flow patterns using more than one column with serial coupling of separation columns
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/38—Flow patterns
- G01N30/46—Flow patterns using more than one column
- G01N30/461—Flow patterns using more than one column with serial coupling of separation columns
- G01N30/463—Flow patterns using more than one column with serial coupling of separation columns for multidimensional chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/38—Flow patterns
- G01N30/46—Flow patterns using more than one column
- G01N30/468—Flow patterns using more than one column involving switching between different column configurations
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/72—Mass spectrometers
- G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/105831—Protein or peptide standard or control [e.g., hemoglobin, etc.]
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/13—Tracers or tags
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/24—Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/25125—Digestion or removing interfering materials
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
- Plural Heterocyclic Compounds (AREA)
- Peptides Or Proteins (AREA)
Abstract
La présente invention concerne de nouveaux composés, désignés agents d'extraction à code isotopique instable vis-à-vis de l'acide (ALICE) destinés à l'analyse spectrométrique de masse quantitative des mélanges de protéines. Les composés comprennent un groupe réactif au thiol utilisé pour la capture des peptides contenant de la cystéine à partir de mélanges de peptides, un lieur instable à l'acide, et un polymère non biologique. Un des deux lieurs instables à l'acide est isotopiquement marqué et permet donc la quantification directe des peptides/protéines par l'analyse spectrométrique de masse. Etant donné qu'il n'est pas nécessaire d'utiliser des protéines fonctionnelles pour la capture des peptides, on peut utiliser un pourcentage supérieur de solvant pour la solubilisation des peptides, notamment des peptides hydrophobes, grâce à des étapes de liaison, de lavage et d'élution, permettant ainsi une bien meilleure récupération des peptides. Par ailleurs, étant donné que les peptides sont liés par covalence au polymère non-biologique (ALICE), on peut effectuer un lavage plus rigoureux afin d'enlever complètement les espèces qui ne sont pas liées de manière spécifique. Enfin, on peut aisément effectuer l'élution des peptides capturés par ALICE à partir du support polymérique dans des conditions d'acidité moyenne avec un rendement élevé et ceci permet une analyse spectrométrique de masse directe en aval sans qu'une manipulation additionnelle d'échantillons ne soit nécessaire. En combinaison avec le nouveau concept de chromatographie liquide-spectrométrie de masse (2D-LC-MS/MS) bidimensionnelle à deux colonnes, le procédé ALICE constitue une approche globale à l'analyse spectrométrique de masse quantitative de mélanges de protéines présentant une meilleure dynamique de mesure et une meilleure sensibilité.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US24264300P | 2000-10-23 | 2000-10-23 | |
US60/242,643 | 2000-10-23 | ||
PCT/US2001/050745 WO2002048717A2 (fr) | 2000-10-23 | 2001-10-22 | Agent d'extraction a code isotopique instable vis-a-vis de l'acide et son utilisation dans l'analyse spectrometrique de masse quantitative de melanges de proteines |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2426731A1 true CA2426731A1 (fr) | 2002-06-20 |
Family
ID=22915614
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002426731A Abandoned CA2426731A1 (fr) | 2000-10-23 | 2001-10-22 | Agent d'extraction a code isotopique instable vis-a-vis de l'acide et son utilisation dans l'analyse spectrometrique de masse quantitative de melanges de proteines |
Country Status (8)
Country | Link |
---|---|
US (2) | US6902936B2 (fr) |
EP (1) | EP1330654B1 (fr) |
JP (1) | JP2004531694A (fr) |
AT (1) | ATE320007T1 (fr) |
AU (2) | AU4338502A (fr) |
CA (1) | CA2426731A1 (fr) |
DE (1) | DE60117849T2 (fr) |
WO (1) | WO2002048717A2 (fr) |
Families Citing this family (31)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2426731A1 (fr) * | 2000-10-23 | 2002-06-20 | Genetics Institute, Llc. | Agent d'extraction a code isotopique instable vis-a-vis de l'acide et son utilisation dans l'analyse spectrometrique de masse quantitative de melanges de proteines |
ATE405839T1 (de) | 2000-10-23 | 2008-09-15 | Genetics Inst Llc | Isotop-kodierte ionisierung-förderende reagenzien zur hochdurchsatz protein identifikation und quantifikation mit massenspectrometrie |
US7183116B2 (en) | 2001-05-14 | 2007-02-27 | The Institute For Systems Biology | Methods for isolation and labeling of sample molecules |
EP1475632B1 (fr) * | 2002-02-14 | 2013-05-22 | Ajinomoto Co., Inc. | Procede d'analyse aminofonctionnelle et reactif analytique |
US7422865B2 (en) * | 2003-01-13 | 2008-09-09 | Agilent Technologies, Inc. | Method of identifying peptides in a proteomic sample |
WO2005000226A2 (fr) * | 2003-06-06 | 2005-01-06 | Diversa Corporation | Systemes de chromatographie multi-dimensionnels a lit melange et procedes de fabrication et d'utilisation associes |
GB0314209D0 (en) * | 2003-06-19 | 2003-07-23 | Amersham Biosciences Ab | Novel MS reagents |
WO2005040197A1 (fr) * | 2003-10-24 | 2005-05-06 | Invitrogen Corporation | Compositions, procedes et necessaires de marquage par fluorophores biarsenicaux |
EP1711833A2 (fr) * | 2004-02-06 | 2006-10-18 | Applera Corporation | Preparation de fluides d'origine biologique pour la determination de biomarqueurs par spectrometrie de masse |
US20070054345A1 (en) | 2004-05-19 | 2007-03-08 | Hunter Christie L | Expression quantification using mass spectrometry |
US20060154318A1 (en) * | 2004-06-09 | 2006-07-13 | Anderson Norman L | Stable isotope labeled polypeptide standards for protein quantitation |
US20070048752A1 (en) | 2004-07-12 | 2007-03-01 | Applera Corporation | Mass tags for quantitative analyses |
US20060172319A1 (en) * | 2004-07-12 | 2006-08-03 | Applera Corporation | Mass tags for quantitative analyses |
US7700364B2 (en) * | 2004-10-12 | 2010-04-20 | Quest Diagnostics Investments Incorporated | Analysis of amino acids in body fluid by liquid chromatography-mass spectrometry |
US8969089B2 (en) * | 2004-10-12 | 2015-03-03 | Quest Diagnostics Investments, Inc. | Analysis of amino acids in body fluid by liquid chromatography-mass spectrometry |
US20060183238A1 (en) | 2005-02-09 | 2006-08-17 | Applera Corporation | Amine-containing compound analysis methods |
EP3543691A1 (fr) * | 2007-06-29 | 2019-09-25 | Quest Diagnostics Investments Incorporated | Analyse des acides aminés dans un liquide corporel par chromatographie en phase liquide/spectrométrie de masse |
KR20110084182A (ko) * | 2008-09-09 | 2011-07-21 | 드 슈타트 데르 네덜란덴, 베르테겐부어디그트 두어 드 미니스터 반 폭스겐트존하이트 벨지인 엔 스포츠 | Lcms 기술 및 이의 용도 |
EP2694528A1 (fr) * | 2011-04-05 | 2014-02-12 | Martin-Protean, LLC | Isolement de peptides contenant de la cystéine |
GB2577626B (en) | 2011-06-23 | 2020-09-23 | Univ Texas | Identifying peptides at the single molecule level |
US11435358B2 (en) | 2011-06-23 | 2022-09-06 | Board Of Regents, The University Of Texas System | Single molecule peptide sequencing |
WO2013078222A1 (fr) | 2011-11-23 | 2013-05-30 | Quest Diagnostics Investments Incorporated | Détection de la kisspeptine-54 par spectrométrie de masse en tandem |
JP6296533B2 (ja) * | 2013-09-03 | 2018-03-20 | 株式会社 資生堂 | システイン及びシスチンの定量方法 |
CN103743851B (zh) * | 2014-02-14 | 2015-06-17 | 中国农业科学院油料作物研究所 | 一种食用油中甘油三酯的单柱二维液相色谱-质谱分析方法及其应用 |
CA2961493C (fr) | 2014-09-15 | 2023-10-03 | Board Of Regents, The University Of Texas System | Sequencage ameliore des peptides d'une seule molecule |
CN107179378B (zh) * | 2016-03-11 | 2018-08-21 | 中国科学院大连化学物理研究所 | 一种同时分离代谢组和脂质组组份的液相色谱分析方法及其应用 |
CN106841404B (zh) * | 2017-01-25 | 2019-11-05 | 苏州大学 | 一种高温全二维液相色谱装置及其使用方法 |
US20210311069A1 (en) * | 2018-08-21 | 2021-10-07 | The Board Of Trustees Of The Leland Stanford Junior University | Isotopically-encoded nanoparticles for multimodal high-order multiplexed detection and imaging |
CN113376286B (zh) * | 2021-05-14 | 2022-03-04 | 谱天(天津)生物科技有限公司 | 一种评估提取纯化方法亲疏水偏好性的方法 |
CN113267554A (zh) * | 2021-05-14 | 2021-08-17 | 谱天(天津)生物科技有限公司 | 一种用于评估蛋白质组还原烷基化效率的质控方法 |
CN114740121A (zh) * | 2022-04-24 | 2022-07-12 | 杭州广科安德生物科技有限公司 | 基于一级质谱分析的双氨基酸稳定同位素标记方法及应用 |
Family Cites Families (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4847325A (en) * | 1988-01-20 | 1989-07-11 | Cetus Corporation | Conjugation of polymer to colony stimulating factor-1 |
US4847324A (en) * | 1988-04-25 | 1989-07-11 | Hydromer, Inc. | Hydrophilic polyvinylbutyral alloys |
US5045694A (en) | 1989-09-27 | 1991-09-03 | The Rockefeller University | Instrument and method for the laser desorption of ions in mass spectrometry |
US4977320A (en) | 1990-01-22 | 1990-12-11 | The Rockefeller University | Electrospray ionization mass spectrometer with new features |
US5288644A (en) | 1990-04-04 | 1994-02-22 | The Rockefeller University | Instrument and method for the sequencing of genome |
US5117009A (en) | 1990-08-31 | 1992-05-26 | University Of Minnesota | Xanthenylamide handle for use in peptide synthesis |
US5245186A (en) | 1991-11-18 | 1993-09-14 | The Rockefeller University | Electrospray ion source for mass spectrometry |
US5792664A (en) | 1992-05-29 | 1998-08-11 | The Rockefeller University | Methods for producing and analyzing biopolymer ladders |
DE69334274D1 (de) | 1992-05-29 | 2009-05-14 | Univ Rockefeller | Verfahren zur Bestimmung der Folge von Peptiden unter Verwendung eines Massenspektrometers |
US6207370B1 (en) * | 1997-09-02 | 2001-03-27 | Sequenom, Inc. | Diagnostics based on mass spectrometric detection of translated target polypeptides |
JP2002521319A (ja) * | 1998-07-23 | 2002-07-16 | メタファー・インコーポレイテッド | ポリヒドロキサメートおよびそのアナログのライブラリー |
ATE253126T1 (de) | 1998-08-25 | 2003-11-15 | Univ Washington | Schnelle quantitative analyse von proteinen oder proteinfunktionen in komplexen gemischen |
EP1059531A1 (fr) | 1999-06-09 | 2000-12-13 | Alfred Prof. Dr. Nordheim | Marquage des peptides et des protéines |
WO2001062827A2 (fr) * | 2000-02-22 | 2001-08-30 | Shearwater Corporation | Derives de polymere n-maleimidyle |
ATE405839T1 (de) | 2000-10-23 | 2008-09-15 | Genetics Inst Llc | Isotop-kodierte ionisierung-förderende reagenzien zur hochdurchsatz protein identifikation und quantifikation mit massenspectrometrie |
CA2426731A1 (fr) * | 2000-10-23 | 2002-06-20 | Genetics Institute, Llc. | Agent d'extraction a code isotopique instable vis-a-vis de l'acide et son utilisation dans l'analyse spectrometrique de masse quantitative de melanges de proteines |
-
2001
- 2001-10-22 CA CA002426731A patent/CA2426731A1/fr not_active Abandoned
- 2001-10-22 DE DE60117849T patent/DE60117849T2/de not_active Expired - Fee Related
- 2001-10-22 WO PCT/US2001/050745 patent/WO2002048717A2/fr active IP Right Grant
- 2001-10-22 JP JP2002549971A patent/JP2004531694A/ja not_active Ceased
- 2001-10-22 AT AT01989278T patent/ATE320007T1/de not_active IP Right Cessation
- 2001-10-22 EP EP01989278A patent/EP1330654B1/fr not_active Expired - Lifetime
- 2001-10-22 AU AU4338502A patent/AU4338502A/xx active Pending
- 2001-10-22 AU AU2002243385A patent/AU2002243385C1/en not_active Ceased
- 2001-10-22 US US10/045,170 patent/US6902936B2/en not_active Expired - Fee Related
-
2004
- 2004-08-17 US US10/919,365 patent/US7122377B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
ATE320007T1 (de) | 2006-03-15 |
DE60117849T2 (de) | 2006-09-21 |
EP1330654B1 (fr) | 2006-03-08 |
US6902936B2 (en) | 2005-06-07 |
JP2004531694A (ja) | 2004-10-14 |
AU2002243385B2 (en) | 2007-07-05 |
AU4338502A (en) | 2002-06-24 |
EP1330654A2 (fr) | 2003-07-30 |
WO2002048717A3 (fr) | 2003-05-01 |
AU2002243385C1 (en) | 2008-07-24 |
DE60117849D1 (de) | 2006-05-04 |
US7122377B2 (en) | 2006-10-17 |
WO2002048717A2 (fr) | 2002-06-20 |
US20020164809A1 (en) | 2002-11-07 |
US20050014278A1 (en) | 2005-01-20 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
FZDE | Discontinued |