CA2401954A1 - Plantes transgeniques sans marqueurs: manipulation du genome chloroplastique sans selection antibiotique - Google Patents
Plantes transgeniques sans marqueurs: manipulation du genome chloroplastique sans selection antibiotique Download PDFInfo
- Publication number
- CA2401954A1 CA2401954A1 CA002401954A CA2401954A CA2401954A1 CA 2401954 A1 CA2401954 A1 CA 2401954A1 CA 002401954 A CA002401954 A CA 002401954A CA 2401954 A CA2401954 A CA 2401954A CA 2401954 A1 CA2401954 A1 CA 2401954A1
- Authority
- CA
- Canada
- Prior art keywords
- plant
- plastid
- aldehyde
- dna sequence
- phytotoxic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8214—Plastid transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Catching Or Destruction (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
La présente invention concerne un procédé permettant de contourner le problème de l'utilisation de marqueurs sélectionnables résistants aux antibiotiques. En particulier, des plantes cibles sont transformées à l'aide d'un vecteur de plastes contenant des séquences d'ADN hétérologue codant une enzyme ou une protéine de détoxification de phytotoxine. Le procédé de sélection consiste à transformer un agent phytotoxique sans antibiotiques à l'aide de l'enzyme ou de la protéine de détoxification de phytotoxine exprimée afin d'obtenir un composé non toxique. L'invention concerne de nombreux procédés d'utilisation de la sélection sans antibiotiques dans la transformation chloroplastique.
Applications Claiming Priority (9)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US18630800P | 2000-03-02 | 2000-03-02 | |
US60/186,308 | 2000-03-02 | ||
US20876300P | 2000-06-02 | 2000-06-02 | |
US60/208,763 | 2000-06-04 | ||
US25740600P | 2000-12-22 | 2000-12-22 | |
US60/257,406 | 2000-12-22 | ||
US25915400P | 2000-12-28 | 2000-12-28 | |
US60/259,154 | 2000-12-29 | ||
PCT/US2001/006275 WO2001064023A1 (fr) | 2000-03-02 | 2001-02-28 | Plantes transgeniques sans marqueurs: manipulation du genome chloroplastique sans selection antibiotique |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2401954A1 true CA2401954A1 (fr) | 2001-09-07 |
Family
ID=27497669
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002401954A Abandoned CA2401954A1 (fr) | 2000-03-02 | 2001-02-28 | Plantes transgeniques sans marqueurs: manipulation du genome chloroplastique sans selection antibiotique |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP1294221A4 (fr) |
AU (1) | AU2001245359A1 (fr) |
CA (1) | CA2401954A1 (fr) |
WO (1) | WO2001064023A1 (fr) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8581035B2 (en) | 2006-08-31 | 2013-11-12 | Monsanto Technology Llc | Plant transformation without selection |
Families Citing this family (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20100251425A9 (en) | 1998-05-15 | 2010-09-30 | University Of Central Florida | Expression of human interferon in transgenic chloroplasts |
ATE510916T1 (de) | 2000-03-01 | 2011-06-15 | Univ Auburn | Plastidtransformationsvektoren zur expression von proinsulin, das an die cholera toxin b- untereinheit fusioniert ist, in pflanzen |
AU2002358292B2 (en) | 2001-12-26 | 2008-12-18 | University Of Central Florida | Expression of protective antigens in transgenic chloroplasts and the production of improved vaccines |
CN102634540B (zh) * | 2002-07-03 | 2015-04-08 | 中佛罗里达大学 | 经由体细胞胚胎发生的质体遗传工程 |
EP1527183B1 (fr) | 2002-07-26 | 2008-08-20 | BASF Plant Science GmbH | Nouveaux procedes de selection |
AU2003285818A1 (en) * | 2002-11-28 | 2004-06-18 | Universiteit Leiden | A method for marker-less integration of a sequence of interest into the genome of a cell |
US20040210961A1 (en) | 2003-03-07 | 2004-10-21 | Palys Joseph Michael | Markerless transformation |
US20090007294A1 (en) * | 2004-09-01 | 2009-01-01 | Henry Daniell | Genetic Engineering of Male Sterility in Plants |
US20080274143A1 (en) | 2005-05-27 | 2008-11-06 | Henry Daniell | Chloroplasts Engineering to Express Pharmaceutical Proteins |
CA2619657C (fr) | 2005-09-16 | 2016-01-26 | Bayer Cropscience Sa | Plantes transplastomiques exprimant une proteine a destination du lumen |
GB2447416B (en) * | 2006-12-15 | 2010-08-25 | Uni I Stavanger | Methods and vectors for transformation of plastids and marker excision using site-specific recombination |
CA2682199C (fr) | 2007-03-30 | 2016-06-28 | University Of Central Florida Research Foundation, Inc. | Chloroplastes genetiquement modifies afin d'exprimer des proteines pharmaceutiques dans des plantes comestibles |
WO2015058214A1 (fr) | 2013-10-18 | 2015-04-23 | Trustees Of The University Of Pennsylvania | Administration orale d'enzyme de conversion de l'angiotensine 2 (ace2) ou d'angiotensine-(1-7) bioencapsulée dans des cellules végétales |
PL3068869T3 (pl) | 2013-11-15 | 2021-03-08 | The Trustees Of The University Of Pennsylvania | Kompozycje do hamowania wytwarzania inhibitorów przeciwko czynnikowi viii u pacjentów z hemofilią a |
MA41180A (fr) | 2014-12-17 | 2017-10-24 | Bayer Cropscience Nv | Plantes caractérisées par une capacité améliorée de fixation du carbone photosynthétique |
JP6696806B2 (ja) * | 2015-08-28 | 2020-05-20 | 本田技研工業株式会社 | プラスチド形質転換体の製造方法 |
WO2017038835A2 (fr) * | 2015-08-28 | 2017-03-09 | Honda Motor Co., Ltd. | Procédé de production de transformants plastidiaux |
CA3043995A1 (fr) | 2015-11-16 | 2017-05-26 | The Trustees Of The University Of Pennsylvania | Liberation ciblee dans des types de cellules d'interet de proteines therapeutiques bioencapsulees dans des cellules vegetales pour le traitement de maladies |
CN109554445B (zh) * | 2019-01-23 | 2021-09-21 | 山东省花生研究所(山东省农业科学院花生工程技术研究中心) | 一种解析花生属种间遗传关系有效简易的方法 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5877402A (en) * | 1990-05-01 | 1999-03-02 | Rutgers, The State University Of New Jersey | DNA constructs and methods for stably transforming plastids of multicellular plants and expressing recombinant proteins therein |
US5633153A (en) * | 1994-10-14 | 1997-05-27 | Calgene, Inc. | Aldehyde dehydrogenase selectable markers for plant transformation |
ES2244073T5 (es) * | 1997-08-07 | 2009-02-01 | Auburn University | Vectores universales de integracion y de expresion de cloroplastos, y plantas transformadas. |
ES2281183T3 (es) * | 1998-07-10 | 2007-09-16 | Calgene Llc | Expresion de genes de tolerancia a herbicida en plastidios vegetales. |
-
2001
- 2001-02-28 AU AU2001245359A patent/AU2001245359A1/en not_active Abandoned
- 2001-02-28 EP EP01918263A patent/EP1294221A4/fr not_active Ceased
- 2001-02-28 CA CA002401954A patent/CA2401954A1/fr not_active Abandoned
- 2001-02-28 WO PCT/US2001/006275 patent/WO2001064023A1/fr active Search and Examination
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8581035B2 (en) | 2006-08-31 | 2013-11-12 | Monsanto Technology Llc | Plant transformation without selection |
US8847009B2 (en) | 2006-08-31 | 2014-09-30 | Monsanto Technology Llc | Plant transformation without selection |
US9617552B2 (en) | 2006-08-31 | 2017-04-11 | Monsanto Technology Llc | Plant transformation without selection |
US10233455B2 (en) | 2006-08-31 | 2019-03-19 | Monsanto Technology Llc | Plant transformation without selection |
US10941407B2 (en) | 2006-08-31 | 2021-03-09 | Monsanto Technology Llc | Plant transformation without selection |
Also Published As
Publication number | Publication date |
---|---|
WO2001064023A1 (fr) | 2001-09-07 |
EP1294221A4 (fr) | 2003-06-25 |
EP1294221A1 (fr) | 2003-03-26 |
AU2001245359A1 (en) | 2001-09-12 |
WO2001064023B1 (fr) | 2001-12-13 |
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DUFOURMANTEL | CHLOROPLAST GENETIC ENGINEERING |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
FZDE | Discontinued |