AU2001245359A1 - Marker free transgenic plants: engineering the chloroplast genome without the use of antibiotic selection - Google Patents

Marker free transgenic plants: engineering the chloroplast genome without the use of antibiotic selection

Info

Publication number
AU2001245359A1
AU2001245359A1 AU2001245359A AU4535901A AU2001245359A1 AU 2001245359 A1 AU2001245359 A1 AU 2001245359A1 AU 2001245359 A AU2001245359 A AU 2001245359A AU 4535901 A AU4535901 A AU 4535901A AU 2001245359 A1 AU2001245359 A1 AU 2001245359A1
Authority
AU
Australia
Prior art keywords
plant
aldehyde
plastid
phytotoxic
chloroplast
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001245359A
Other languages
English (en)
Inventor
Henry Daniell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Auburn University
University of Central Florida
Original Assignee
Auburn University
University of Central Florida
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Auburn University, University of Central Florida filed Critical Auburn University
Publication of AU2001245359A1 publication Critical patent/AU2001245359A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214Plastid transformation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Catching Or Destruction (AREA)
  • Pretreatment Of Seeds And Plants (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
AU2001245359A 2000-03-02 2001-02-28 Marker free transgenic plants: engineering the chloroplast genome without the use of antibiotic selection Abandoned AU2001245359A1 (en)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US18630800P 2000-03-02 2000-03-02
US60186308 2000-03-02
US20876300P 2000-06-02 2000-06-02
US60208763 2000-06-04
US25740600P 2000-12-22 2000-12-22
US60257406 2000-12-22
US25915400P 2000-12-28 2000-12-28
US60259154 2000-12-29
PCT/US2001/006275 WO2001064023A1 (fr) 2000-03-02 2001-02-28 Plantes transgeniques sans marqueurs: manipulation du genome chloroplastique sans selection antibiotique

Publications (1)

Publication Number Publication Date
AU2001245359A1 true AU2001245359A1 (en) 2001-09-12

Family

ID=27497669

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001245359A Abandoned AU2001245359A1 (en) 2000-03-02 2001-02-28 Marker free transgenic plants: engineering the chloroplast genome without the use of antibiotic selection

Country Status (4)

Country Link
EP (1) EP1294221A4 (fr)
AU (1) AU2001245359A1 (fr)
CA (1) CA2401954A1 (fr)
WO (1) WO2001064023A1 (fr)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100251425A9 (en) 1998-05-15 2010-09-30 University Of Central Florida Expression of human interferon in transgenic chloroplasts
ATE510916T1 (de) 2000-03-01 2011-06-15 Univ Auburn Plastidtransformationsvektoren zur expression von proinsulin, das an die cholera toxin b- untereinheit fusioniert ist, in pflanzen
AU2002358292B2 (en) 2001-12-26 2008-12-18 University Of Central Florida Expression of protective antigens in transgenic chloroplasts and the production of improved vaccines
CN102634540B (zh) * 2002-07-03 2015-04-08 中佛罗里达大学 经由体细胞胚胎发生的质体遗传工程
EP1527183B1 (fr) 2002-07-26 2008-08-20 BASF Plant Science GmbH Nouveaux procedes de selection
AU2003285818A1 (en) * 2002-11-28 2004-06-18 Universiteit Leiden A method for marker-less integration of a sequence of interest into the genome of a cell
US20040210961A1 (en) 2003-03-07 2004-10-21 Palys Joseph Michael Markerless transformation
US20090007294A1 (en) * 2004-09-01 2009-01-01 Henry Daniell Genetic Engineering of Male Sterility in Plants
US20080274143A1 (en) 2005-05-27 2008-11-06 Henry Daniell Chloroplasts Engineering to Express Pharmaceutical Proteins
CA2619657C (fr) 2005-09-16 2016-01-26 Bayer Cropscience Sa Plantes transplastomiques exprimant une proteine a destination du lumen
WO2008028119A2 (fr) 2006-08-31 2008-03-06 Monsanto Technology Llc. Procédés pour transformer rapidement des monocotylédones
GB2447416B (en) * 2006-12-15 2010-08-25 Uni I Stavanger Methods and vectors for transformation of plastids and marker excision using site-specific recombination
CA2682199C (fr) 2007-03-30 2016-06-28 University Of Central Florida Research Foundation, Inc. Chloroplastes genetiquement modifies afin d'exprimer des proteines pharmaceutiques dans des plantes comestibles
WO2015058214A1 (fr) 2013-10-18 2015-04-23 Trustees Of The University Of Pennsylvania Administration orale d'enzyme de conversion de l'angiotensine 2 (ace2) ou d'angiotensine-(1-7) bioencapsulée dans des cellules végétales
PL3068869T3 (pl) 2013-11-15 2021-03-08 The Trustees Of The University Of Pennsylvania Kompozycje do hamowania wytwarzania inhibitorów przeciwko czynnikowi viii u pacjentów z hemofilią a
MA41180A (fr) 2014-12-17 2017-10-24 Bayer Cropscience Nv Plantes caractérisées par une capacité améliorée de fixation du carbone photosynthétique
JP6696806B2 (ja) * 2015-08-28 2020-05-20 本田技研工業株式会社 プラスチド形質転換体の製造方法
WO2017038835A2 (fr) * 2015-08-28 2017-03-09 Honda Motor Co., Ltd. Procédé de production de transformants plastidiaux
CA3043995A1 (fr) 2015-11-16 2017-05-26 The Trustees Of The University Of Pennsylvania Liberation ciblee dans des types de cellules d'interet de proteines therapeutiques bioencapsulees dans des cellules vegetales pour le traitement de maladies
CN109554445B (zh) * 2019-01-23 2021-09-21 山东省花生研究所(山东省农业科学院花生工程技术研究中心) 一种解析花生属种间遗传关系有效简易的方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5877402A (en) * 1990-05-01 1999-03-02 Rutgers, The State University Of New Jersey DNA constructs and methods for stably transforming plastids of multicellular plants and expressing recombinant proteins therein
US5633153A (en) * 1994-10-14 1997-05-27 Calgene, Inc. Aldehyde dehydrogenase selectable markers for plant transformation
ES2244073T5 (es) * 1997-08-07 2009-02-01 Auburn University Vectores universales de integracion y de expresion de cloroplastos, y plantas transformadas.
ES2281183T3 (es) * 1998-07-10 2007-09-16 Calgene Llc Expresion de genes de tolerancia a herbicida en plastidios vegetales.

Also Published As

Publication number Publication date
WO2001064023A1 (fr) 2001-09-07
EP1294221A4 (fr) 2003-06-25
CA2401954A1 (fr) 2001-09-07
EP1294221A1 (fr) 2003-03-26
WO2001064023B1 (fr) 2001-12-13

Similar Documents

Publication Publication Date Title
US20110072541A1 (en) Marker free transgenic plants engineering the chloroplast genome without the use of antibiotic selection
Daniell et al. Marker free transgenic plants: engineering the chloroplast genome without the use of antibiotic selection
CA2299609C (fr) Vecteurs universels d'integration et d'expression de chloroplastes, plantes transformees et produits obtenus
AU2001245359A1 (en) Marker free transgenic plants: engineering the chloroplast genome without the use of antibiotic selection
Grevich et al. Chloroplast genetic engineering: recent advances and future perspectives
US7129391B1 (en) Universal chloroplast integration and expression vectors, transformed plants and products thereof
Lee et al. Plastid transformation in the monocotyledonous cereal crop, rice (Oryza sativa) and transmission of transgenes to their progeny
Kumar et al. Plastid-expressed betaine aldehyde dehydrogenase gene in carrot cultured cells, roots, and leaves confers enhanced salt tolerance
US7294506B2 (en) Tobacco chloroplast transformation vectors comprising a multi-gene operon encoding a biopharmaceutical protein and a chaperonin
Daniell et al. Containment of herbicide resistance through genetic engineering of the chloroplast genome
Verma et al. Chloroplast vector systems for biotechnology applications
You et al. Sweet pepper ferredoxin-like protein (pflp) gene as a novel selection marker for orchid transformation
FR2848568A1 (fr) Gene chimere permettant l'expression d'une hydroxy-phenyl pyruvate dioxygenase dans les plastes et plantes transplastomiques contenant un tel gene tolerantes aux herbicides
Daniell et al. Chloroplast genetic engineering
EP1263281B1 (fr) Expression de genes multiples pour la mise au point de nouvelles voies et hyperexpression de proteines etrangeres dans des plantes
Devine et al. Chloroplast genetic engineering for enhanced agronomic traits and expression of proteins for medical/industrial applications
EP1571220B1 (fr) Vecteurs universels d'intégration et d'expression de chloroplastes, plantes transformées et produits obtenus
RU2238324C2 (ru) Универсальный вектор для стабильной трансформации хлоропластного генома, способ стабильной трансформации растения-мишени, способ придания устойчивости к гербициду растению-мишени, способ определения трансформации хлоропласта и стабильно трансформированный хлоропластный геном
Koya et al. OBPC Symposium: Maize 2004 & Beyond—recent advances in chloroplast genetic engineering
Grevich Bioinformatic Analysis Of Solanaceae Chloroplast Genomes And Characterization Of An Arabidopsis Protein Disulfide Isomerase In Transgenic Tobacco Chloroplasts
DUFOURMANTEL CHLOROPLAST GENETIC ENGINEERING
Chung et al. Agrobacterium Mediated Transformation of Rehmannia glutinosa L. with Glutathione S—Transferase Gene (Gh-5)
Kim Genetic engineering of Populus species to improve resistance to environmental stresses
Holsters et al. Present plant biotechnology
MXPA00001341A (en) Universal chloroplast integration and expression vectors, transformed plants and products thereof