CA2401621A1 - Modulation of the activity of the endothelial cel surface receptor ecrtp/dep-1 in the regulation of angiogenesis - Google Patents

Info

Publication number

CA2401621A1

CA2401621A1 CA002401621A CA2401621A CA2401621A1 CA 2401621 A1 CA2401621 A1 CA 2401621A1 CA 002401621 A CA002401621 A CA 002401621A CA 2401621 A CA2401621 A CA 2401621A CA 2401621 A1 CA2401621 A1 CA 2401621A1

Authority

CA

Canada

Prior art keywords

ecrtp

dep

antibody

cells

cell

Prior art date

2000-03-01

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Abandoned

Links

• Espacenet
• Global Dossier
• CIPO
• Discuss

• 230000000694 effects Effects 0.000 title claims description 109
• 230000003511 endothelial effect Effects 0.000 title description 45
• 230000006884 regulation of angiogenesis Effects 0.000 title description 4
• 101100277553 Caenorhabditis elegans dep-1 gene Proteins 0.000 title description 2
• 238000000034 method Methods 0.000 claims abstract description 165
• 230000033115 angiogenesis Effects 0.000 claims abstract description 79
• 101000606506 Homo sapiens Receptor-type tyrosine-protein phosphatase eta Proteins 0.000 claims abstract description 45
• 102100039808 Receptor-type tyrosine-protein phosphatase eta Human genes 0.000 claims abstract description 45
• 238000012216 screening Methods 0.000 claims abstract description 30
• 210000004027 cell Anatomy 0.000 claims description 261
• 108090000765 processed proteins & peptides Proteins 0.000 claims description 121
• 102000004196 processed proteins & peptides Human genes 0.000 claims description 92
• 239000003446 ligand Substances 0.000 claims description 76
• 229920001184 polypeptide Polymers 0.000 claims description 65
• 230000027455 binding Effects 0.000 claims description 60
• 239000000126 substance Substances 0.000 claims description 60
• 150000001875 compounds Chemical class 0.000 claims description 51
• 230000012010 growth Effects 0.000 claims description 51
• 238000012360 testing method Methods 0.000 claims description 50
• 150000001413 amino acids Chemical class 0.000 claims description 44
• 239000012634 fragment Substances 0.000 claims description 44
• 108091008600 receptor tyrosine phosphatases Proteins 0.000 claims description 41
• 238000003556 assay Methods 0.000 claims description 39
• 108020003175 receptors Proteins 0.000 claims description 27
• 230000004071 biological effect Effects 0.000 claims description 24
• 210000004408 hybridoma Anatomy 0.000 claims description 24
• 230000003993 interaction Effects 0.000 claims description 21
• 239000000523 sample Substances 0.000 claims description 21
• 230000004663 cell proliferation Effects 0.000 claims description 18
• 239000003153 chemical reaction reagent Substances 0.000 claims description 13
• 108020004414 DNA Proteins 0.000 claims description 12
• 230000015572 biosynthetic process Effects 0.000 claims description 12
• 239000007790 solid phase Substances 0.000 claims description 12
• 230000010595 endothelial cell migration Effects 0.000 claims description 10
• 239000002299 complementary DNA Substances 0.000 claims description 9
• 239000013592 cell lysate Substances 0.000 claims description 8
• DCWXELXMIBXGTH-QMMMGPOBSA-N phosphonotyrosine Chemical group OC(=O)[C@@H](N)CC1=CC=C(OP(O)(O)=O)C=C1 DCWXELXMIBXGTH-QMMMGPOBSA-N 0.000 claims description 8
• 230000036046 immunoreaction Effects 0.000 claims description 7
• 239000008194 pharmaceutical composition Substances 0.000 claims description 7
• 230000009870 specific binding Effects 0.000 claims description 7
• 102000045595 Phosphoprotein Phosphatases Human genes 0.000 claims description 5
• 239000013068 control sample Substances 0.000 claims description 5
• 239000000546 pharmaceutical excipient Substances 0.000 claims description 5
• 102000004190 Enzymes Human genes 0.000 claims description 4
• 108090000790 Enzymes Proteins 0.000 claims description 4
• 239000011159 matrix material Substances 0.000 claims description 4
• 150000001408 amides Chemical class 0.000 claims description 3
• 239000003085 diluting agent Substances 0.000 claims description 3
• 239000003937 drug carrier Substances 0.000 claims description 3
• 230000002285 radioactive effect Effects 0.000 claims description 3
• 238000012163 sequencing technique Methods 0.000 claims description 3
• 108091028043 Nucleic acid sequence Proteins 0.000 claims description 2
• 230000001747 exhibiting effect Effects 0.000 claims description 2
• 230000002349 favourable effect Effects 0.000 claims description 2
• 230000002934 lysing effect Effects 0.000 claims description 2
• 239000000203 mixture Substances 0.000 abstract description 57
• 241000251539 Vertebrata <Metazoa> Species 0.000 abstract description 14
• 108090000623 proteins and genes Proteins 0.000 description 70
• 210000001519 tissue Anatomy 0.000 description 67
• 210000002889 endothelial cell Anatomy 0.000 description 64
• 239000003795 chemical substances by application Substances 0.000 description 55
• 108700012359 toxins Proteins 0.000 description 52
• 102000004169 proteins and genes Human genes 0.000 description 51
• 239000003053 toxin Substances 0.000 description 50
• 231100000765 toxin Toxicity 0.000 description 50
• 235000018102 proteins Nutrition 0.000 description 48
• 206010028980 Neoplasm Diseases 0.000 description 46
• 230000008685 targeting Effects 0.000 description 46
• 230000014509 gene expression Effects 0.000 description 39
• 238000013508 migration Methods 0.000 description 39
• 229940024606 amino acid Drugs 0.000 description 34
• 230000005012 migration Effects 0.000 description 34
• 235000001014 amino acid Nutrition 0.000 description 33
• 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 31
• 230000035755 proliferation Effects 0.000 description 31
• 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 30
• 210000003734 kidney Anatomy 0.000 description 30
• 210000004379 membrane Anatomy 0.000 description 28
• 239000012528 membrane Substances 0.000 description 28
• 108050007957 Cadherin Proteins 0.000 description 27
• 102000000905 Cadherin Human genes 0.000 description 27
• 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 26
• 102000005962 receptors Human genes 0.000 description 26
• 101710154606 Hemagglutinin Proteins 0.000 description 23
• 101710093908 Outer capsid protein VP4 Proteins 0.000 description 23
• 101710135467 Outer capsid protein sigma-1 Proteins 0.000 description 23
• 101710176177 Protein A56 Proteins 0.000 description 23
• 239000000185 hemagglutinin Substances 0.000 description 23
• 241000699666 Mus <mouse, genus> Species 0.000 description 21
• 230000001225 therapeutic effect Effects 0.000 description 21
• 206010052428 Wound Diseases 0.000 description 20
• 208000027418 Wounds and injury Diseases 0.000 description 20
• 201000010099 disease Diseases 0.000 description 20
• 238000009826 distribution Methods 0.000 description 19
• 230000006870 function Effects 0.000 description 19
• 238000010186 staining Methods 0.000 description 19
• OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 18
• OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 18
• 210000001736 capillary Anatomy 0.000 description 18
• 230000010261 cell growth Effects 0.000 description 18
• OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 18
• 235000002374 tyrosine Nutrition 0.000 description 18
• 239000013598 vector Substances 0.000 description 18
• 125000000539 amino acid group Chemical group 0.000 description 16
• CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 15
• 239000000427 antigen Substances 0.000 description 15
• 108091007433 antigens Proteins 0.000 description 15
• 102000036639 antigens Human genes 0.000 description 15
• 210000004925 microvascular endothelial cell Anatomy 0.000 description 15
• 230000008569 process Effects 0.000 description 15
• -1 HPTPr) Proteins 0.000 description 14
• LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 14
• 230000005764 inhibitory process Effects 0.000 description 14
• 150000007523 nucleic acids Chemical class 0.000 description 14
• 239000002953 phosphate buffered saline Substances 0.000 description 14
• 206010029113 Neovascularisation Diseases 0.000 description 13
• 238000002474 experimental method Methods 0.000 description 13
• 102000037865 fusion proteins Human genes 0.000 description 13
• 108020001507 fusion proteins Proteins 0.000 description 13
• 239000001963 growth medium Substances 0.000 description 13
• 230000001965 increasing effect Effects 0.000 description 13
• 238000007423 screening assay Methods 0.000 description 13
• 230000004083 survival effect Effects 0.000 description 13
• 210000004204 blood vessel Anatomy 0.000 description 12
• 201000011510 cancer Diseases 0.000 description 12
• 239000013604 expression vector Substances 0.000 description 12
• 238000002360 preparation method Methods 0.000 description 12
• 235000002639 sodium chloride Nutrition 0.000 description 12
• WOVKYSAHUYNSMH-RRKCRQDMSA-N 5-bromodeoxyuridine Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(Br)=C1 WOVKYSAHUYNSMH-RRKCRQDMSA-N 0.000 description 11
• 241000283707 Capra Species 0.000 description 11
• 108060003951 Immunoglobulin Proteins 0.000 description 11
• 108010054477 Immunoglobulin Fab Fragments Proteins 0.000 description 11
• 102000001706 Immunoglobulin Fab Fragments Human genes 0.000 description 11
• 238000007792 addition Methods 0.000 description 11
• 238000013459 approach Methods 0.000 description 11
• 230000001434 glomerular Effects 0.000 description 11
• 102000018358 immunoglobulin Human genes 0.000 description 11
• 230000002401 inhibitory effect Effects 0.000 description 11
• 230000001404 mediated effect Effects 0.000 description 11
• 108020004707 nucleic acids Proteins 0.000 description 11
• 102000039446 nucleic acids Human genes 0.000 description 11
• 239000013612 plasmid Substances 0.000 description 11
• 239000000047 product Substances 0.000 description 11
• 230000004044 response Effects 0.000 description 11
• 241000894007 species Species 0.000 description 11
• WOVKYSAHUYNSMH-UHFFFAOYSA-N BROMODEOXYURIDINE Natural products C1C(O)C(CO)OC1N1C(=O)NC(=O)C(Br)=C1 WOVKYSAHUYNSMH-UHFFFAOYSA-N 0.000 description 10
• 239000004971 Cross linker Substances 0.000 description 10
• 108090001090 Lectins Proteins 0.000 description 10
• 102000004856 Lectins Human genes 0.000 description 10
• 241001465754 Metazoa Species 0.000 description 10
• 241000700605 Viruses Species 0.000 description 10
• 230000002491 angiogenic effect Effects 0.000 description 10
• 239000002523 lectin Substances 0.000 description 10
• 230000004807 localization Effects 0.000 description 10
• 239000002609 medium Substances 0.000 description 10
• 230000014399 negative regulation of angiogenesis Effects 0.000 description 10
• 238000006366 phosphorylation reaction Methods 0.000 description 10
• 238000000746 purification Methods 0.000 description 10
• 230000002792 vascular Effects 0.000 description 10
• 229920000936 Agarose Polymers 0.000 description 9
• 241000124008 Mammalia Species 0.000 description 9
• HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 9
• 230000008901 benefit Effects 0.000 description 9
• 210000004369 blood Anatomy 0.000 description 9
• 239000008280 blood Substances 0.000 description 9
• 210000003711 chorioallantoic membrane Anatomy 0.000 description 9
• 210000003038 endothelium Anatomy 0.000 description 9
• 210000005086 glomerual capillary Anatomy 0.000 description 9
• 238000003119 immunoblot Methods 0.000 description 9
• 238000000338 in vitro Methods 0.000 description 9
• 238000002372 labelling Methods 0.000 description 9
• 230000001105 regulatory effect Effects 0.000 description 9
• 238000011160 research Methods 0.000 description 9
• 150000003839 salts Chemical class 0.000 description 9
• 238000001890 transfection Methods 0.000 description 9
• 108091003079 Bovine Serum Albumin Proteins 0.000 description 8
• 241000282412 Homo Species 0.000 description 8
• KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 8
• 206010027476 Metastases Diseases 0.000 description 8
• 241000699670 Mus sp. Species 0.000 description 8
• 241000283973 Oryctolagus cuniculus Species 0.000 description 8
• 239000004480 active ingredient Substances 0.000 description 8
• 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 8
• 230000003197 catalytic effect Effects 0.000 description 8
• 230000004087 circulation Effects 0.000 description 8
• YBYRMVIVWMBXKQ-UHFFFAOYSA-N phenylmethanesulfonyl fluoride Chemical compound FS(=O)(=O)CC1=CC=CC=C1 YBYRMVIVWMBXKQ-UHFFFAOYSA-N 0.000 description 8
• 230000026731 phosphorylation Effects 0.000 description 8
• 125000006239 protecting group Chemical group 0.000 description 8
• 210000002966 serum Anatomy 0.000 description 8
• 239000007787 solid Substances 0.000 description 8
• 238000006467 substitution reaction Methods 0.000 description 8
• 238000002560 therapeutic procedure Methods 0.000 description 8
• 239000004472 Lysine Substances 0.000 description 7
• 108020004511 Recombinant DNA Proteins 0.000 description 7
• 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 7
• 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 7
• 229920002684 Sepharose Polymers 0.000 description 7
• 230000035508 accumulation Effects 0.000 description 7
• 238000009825 accumulation Methods 0.000 description 7
• 239000002253 acid Substances 0.000 description 7
• 239000000872 buffer Substances 0.000 description 7
• 239000006274 endogenous ligand Substances 0.000 description 7
• 239000013613 expression plasmid Substances 0.000 description 7
• 230000003053 immunization Effects 0.000 description 7
• 238000001727 in vivo Methods 0.000 description 7
• 125000005647 linker group Chemical group 0.000 description 7
• 235000018977 lysine Nutrition 0.000 description 7
• 238000004519 manufacturing process Methods 0.000 description 7
• 102000006240 membrane receptors Human genes 0.000 description 7
• 230000002018 overexpression Effects 0.000 description 7
• 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 7
• 239000000243 solution Substances 0.000 description 7
• 125000006850 spacer group Chemical group 0.000 description 7
• 238000011282 treatment Methods 0.000 description 7
• 241000894006 Bacteria Species 0.000 description 6
• KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 6
• 241001529936 Murinae Species 0.000 description 6
• 230000012292 cell migration Effects 0.000 description 6
• 239000003431 cross linking reagent Substances 0.000 description 6
• 230000001086 cytosolic effect Effects 0.000 description 6
• 208000035475 disorder Diseases 0.000 description 6
• 230000002500 effect on skin Effects 0.000 description 6
• 210000002919 epithelial cell Anatomy 0.000 description 6
• 238000002649 immunization Methods 0.000 description 6
• 239000002596 immunotoxin Substances 0.000 description 6
• 230000002637 immunotoxin Effects 0.000 description 6
• 229940051026 immunotoxin Drugs 0.000 description 6
• 231100000608 immunotoxin Toxicity 0.000 description 6
• 239000008188 pellet Substances 0.000 description 6
• 238000007747 plating Methods 0.000 description 6
• 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 6
• 239000000758 substrate Substances 0.000 description 6
• 241000701161 unidentified adenovirus Species 0.000 description 6
• 108010001857 Cell Surface Receptors Proteins 0.000 description 5
• PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 5
• 206010035226 Plasma cell myeloma Diseases 0.000 description 5
• DTQVDTLACAAQTR-UHFFFAOYSA-N Trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-N 0.000 description 5
• 230000009471 action Effects 0.000 description 5
• 239000011230 binding agent Substances 0.000 description 5
• 238000004113 cell culture Methods 0.000 description 5
• 210000000170 cell membrane Anatomy 0.000 description 5
• 238000011161 development Methods 0.000 description 5
• 230000018109 developmental process Effects 0.000 description 5
• 239000003814 drug Substances 0.000 description 5
• 239000012091 fetal bovine serum Substances 0.000 description 5
• 230000003394 haemopoietic effect Effects 0.000 description 5
• 238000011065 in-situ storage Methods 0.000 description 5
• 238000011534 incubation Methods 0.000 description 5
• 239000006166 lysate Substances 0.000 description 5
• 201000000050 myeloid neoplasm Diseases 0.000 description 5
• 238000010647 peptide synthesis reaction Methods 0.000 description 5
• DCWXELXMIBXGTH-UHFFFAOYSA-N phosphotyrosine Chemical compound OC(=O)C(N)CC1=CC=C(OP(O)(O)=O)C=C1 DCWXELXMIBXGTH-UHFFFAOYSA-N 0.000 description 5
• 239000002243 precursor Substances 0.000 description 5
• 239000002356 single layer Substances 0.000 description 5
• 238000013518 transcription Methods 0.000 description 5
• 230000035897 transcription Effects 0.000 description 5
• 230000004614 tumor growth Effects 0.000 description 5
• 210000005166 vasculature Anatomy 0.000 description 5
• XZKIHKMTEMTJQX-UHFFFAOYSA-N 4-Nitrophenyl Phosphate Chemical compound OP(O)(=O)OC1=CC=C([N+]([O-])=O)C=C1 XZKIHKMTEMTJQX-UHFFFAOYSA-N 0.000 description 4
• NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 4
• 108091026890 Coding region Proteins 0.000 description 4
• 102000001189 Cyclic Peptides Human genes 0.000 description 4
• 108010069514 Cyclic Peptides Proteins 0.000 description 4
• AOJJSUZBOXZQNB-TZSSRYMLSA-N Doxorubicin Chemical compound O([C@H]1C[C@@](O)(CC=2C(O)=C3C(=O)C=4C=CC=C(C=4C(=O)C3=C(O)C=21)OC)C(=O)CO)[C@H]1C[C@H](N)[C@H](O)[C@H](C)O1 AOJJSUZBOXZQNB-TZSSRYMLSA-N 0.000 description 4
• KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 4
• 238000002965 ELISA Methods 0.000 description 4
• 102100030779 Ephrin type-B receptor 1 Human genes 0.000 description 4
• WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
• 101001064150 Homo sapiens Ephrin type-B receptor 1 Proteins 0.000 description 4
• VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 4
• ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 4
• HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 4
• FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 description 4
• BAVYZALUXZFZLV-UHFFFAOYSA-N Methylamine Chemical compound NC BAVYZALUXZFZLV-UHFFFAOYSA-N 0.000 description 4
• 101710182846 Polyhedrin Proteins 0.000 description 4
• 241000282887 Suidae Species 0.000 description 4
• 239000013504 Triton X-100 Substances 0.000 description 4
• 229920004890 Triton X-100 Polymers 0.000 description 4
• 150000007513 acids Chemical class 0.000 description 4
• 238000001042 affinity chromatography Methods 0.000 description 4
• 125000003277 amino group Chemical group 0.000 description 4
• 239000002246 antineoplastic agent Substances 0.000 description 4
• 210000002469 basement membrane Anatomy 0.000 description 4
• WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 4
• 230000036770 blood supply Effects 0.000 description 4
• 239000011575 calcium Substances 0.000 description 4
• 239000000969 carrier Substances 0.000 description 4
• 210000004671 cell-free system Anatomy 0.000 description 4
• 230000001413 cellular effect Effects 0.000 description 4
• 210000004978 chinese hamster ovary cell Anatomy 0.000 description 4
• 230000008045 co-localization Effects 0.000 description 4
• 238000012217 deletion Methods 0.000 description 4
• 230000037430 deletion Effects 0.000 description 4
• 230000030609 dephosphorylation Effects 0.000 description 4
• 238000006209 dephosphorylation reaction Methods 0.000 description 4
• 230000004069 differentiation Effects 0.000 description 4
• 238000005516 engineering process Methods 0.000 description 4
• 238000009472 formulation Methods 0.000 description 4
• 239000000499 gel Substances 0.000 description 4
• 239000011521 glass Substances 0.000 description 4
• RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 description 4
• 239000003102 growth factor Substances 0.000 description 4
• HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 4
• JYGXADMDTFJGBT-VWUMJDOOSA-N hydrocortisone Chemical compound O=C1CC[C@]2(C)[C@H]3[C@@H](O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 JYGXADMDTFJGBT-VWUMJDOOSA-N 0.000 description 4
• 238000003384 imaging method Methods 0.000 description 4
• 238000012744 immunostaining Methods 0.000 description 4
• 230000000977 initiatory effect Effects 0.000 description 4
• 210000004698 lymphocyte Anatomy 0.000 description 4
• 229960000485 methotrexate Drugs 0.000 description 4
• 230000004048 modification Effects 0.000 description 4
• 238000012986 modification Methods 0.000 description 4
• 210000000056 organ Anatomy 0.000 description 4
• 238000012545 processing Methods 0.000 description 4
• 210000000952 spleen Anatomy 0.000 description 4
• UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 4
• 238000013519 translation Methods 0.000 description 4
• 230000006459 vascular development Effects 0.000 description 4
• GKSPIZSKQWTXQG-UHFFFAOYSA-N (2,5-dioxopyrrolidin-1-yl) 4-[1-(pyridin-2-yldisulfanyl)ethyl]benzoate Chemical group C=1C=C(C(=O)ON2C(CCC2=O)=O)C=CC=1C(C)SSC1=CC=CC=N1 GKSPIZSKQWTXQG-UHFFFAOYSA-N 0.000 description 3
• JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 3
• QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
• 108010039627 Aprotinin Proteins 0.000 description 3
• 241000271566 Aves Species 0.000 description 3
• UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical group C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
• OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 3
• 108090000695 Cytokines Proteins 0.000 description 3
• 102000004127 Cytokines Human genes 0.000 description 3
• 206010012689 Diabetic retinopathy Diseases 0.000 description 3
• YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 3
• IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
• 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 3
• 102000006397 Ephrin-B1 Human genes 0.000 description 3
• 108010044099 Ephrin-B1 Proteins 0.000 description 3
• 241000283074 Equus asinus Species 0.000 description 3
• LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
• XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
• 108010070675 Glutathione transferase Proteins 0.000 description 3
• 102000005720 Glutathione transferase Human genes 0.000 description 3
• HVLSXIKZNLPZJJ-TXZCQADKSA-N HA peptide Chemical compound C([C@@H](C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](C(C)C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](C)C(O)=O)NC(=O)[C@H]1N(CCC1)C(=O)[C@@H](N)CC=1C=CC(O)=CC=1)C1=CC=C(O)C=C1 HVLSXIKZNLPZJJ-TXZCQADKSA-N 0.000 description 3
• 102000008394 Immunoglobulin Fragments Human genes 0.000 description 3
• 108010021625 Immunoglobulin Fragments Proteins 0.000 description 3
• GDBQQVLCIARPGH-UHFFFAOYSA-N Leupeptin Natural products CC(C)CC(NC(C)=O)C(=O)NC(CC(C)C)C(=O)NC(C=O)CCCN=C(N)N GDBQQVLCIARPGH-UHFFFAOYSA-N 0.000 description 3
• ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 3
• SJRJJKPEHAURKC-UHFFFAOYSA-N N-Methylmorpholine Chemical compound CN1CCOCC1 SJRJJKPEHAURKC-UHFFFAOYSA-N 0.000 description 3
• 108091034117 Oligonucleotide Proteins 0.000 description 3
• MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 description 3
• 239000002033 PVDF binder Substances 0.000 description 3
• KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
• 240000004808 Saccharomyces cerevisiae Species 0.000 description 3
• FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
• 108091081024 Start codon Proteins 0.000 description 3
• 241000282898 Sus scrofa Species 0.000 description 3
• 239000006180 TBST buffer Substances 0.000 description 3
• ZMANZCXQSJIPKH-UHFFFAOYSA-N Triethylamine Chemical compound CCN(CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-N 0.000 description 3
• 102000008790 VE-cadherin Human genes 0.000 description 3
• 108010073929 Vascular Endothelial Growth Factor A Proteins 0.000 description 3
• 102000016549 Vascular Endothelial Growth Factor Receptor-2 Human genes 0.000 description 3
• 108010053099 Vascular Endothelial Growth Factor Receptor-2 Proteins 0.000 description 3
• 102000005789 Vascular Endothelial Growth Factors Human genes 0.000 description 3
• 108010019530 Vascular Endothelial Growth Factors Proteins 0.000 description 3
• 238000004458 analytical method Methods 0.000 description 3
• 239000004037 angiogenesis inhibitor Substances 0.000 description 3
• 229940121369 angiogenesis inhibitor Drugs 0.000 description 3
• 230000006427 angiogenic response Effects 0.000 description 3
• 239000005557 antagonist Substances 0.000 description 3
• 229960004405 aprotinin Drugs 0.000 description 3
• 239000007864 aqueous solution Substances 0.000 description 3
• 210000001367 artery Anatomy 0.000 description 3
• 230000008827 biological function Effects 0.000 description 3
• 230000000903 blocking effect Effects 0.000 description 3
• 108010018828 cadherin 5 Proteins 0.000 description 3
• 229910052791 calcium Inorganic materials 0.000 description 3
• 238000006243 chemical reaction Methods 0.000 description 3
• 230000001684 chronic effect Effects 0.000 description 3
• 238000004624 confocal microscopy Methods 0.000 description 3
• 238000004132 cross linking Methods 0.000 description 3
• 229940127089 cytotoxic agent Drugs 0.000 description 3
• UAOMVDZJSHZZME-UHFFFAOYSA-N diisopropylamine Chemical compound CC(C)NC(C)C UAOMVDZJSHZZME-UHFFFAOYSA-N 0.000 description 3
• 239000000539 dimer Substances 0.000 description 3
• 238000006471 dimerization reaction Methods 0.000 description 3
• 229940079593 drug Drugs 0.000 description 3
• 238000010828 elution Methods 0.000 description 3
• 210000004954 endothelial membrane Anatomy 0.000 description 3
• 239000002158 endotoxin Substances 0.000 description 3
• 229940088598 enzyme Drugs 0.000 description 3
• 238000001317 epifluorescence microscopy Methods 0.000 description 3
• DEFVIWRASFVYLL-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl)tetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O DEFVIWRASFVYLL-UHFFFAOYSA-N 0.000 description 3
• 238000011156 evaluation Methods 0.000 description 3
• 230000000763 evoking effect Effects 0.000 description 3
• 210000002950 fibroblast Anatomy 0.000 description 3
• 230000005714 functional activity Effects 0.000 description 3
• 230000004927 fusion Effects 0.000 description 3
• 238000002523 gelfiltration Methods 0.000 description 3
• 230000013595 glycosylation Effects 0.000 description 3
• 238000006206 glycosylation reaction Methods 0.000 description 3
• 239000005090 green fluorescent protein Substances 0.000 description 3
• GPRLSGONYQIRFK-UHFFFAOYSA-N hydron Chemical compound [H+] GPRLSGONYQIRFK-UHFFFAOYSA-N 0.000 description 3
• RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 description 3
• 230000002163 immunogen Effects 0.000 description 3
• 238000001114 immunoprecipitation Methods 0.000 description 3
• 238000010348 incorporation Methods 0.000 description 3
• 239000003112 inhibitor Substances 0.000 description 3
• ZPNFWUPYTFPOJU-LPYSRVMUSA-N iniprol Chemical compound C([C@H]1C(=O)NCC(=O)NCC(=O)N[C@H]2CSSC[C@H]3C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@H](C(N[C@H](C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=4C=CC(O)=CC=4)C(=O)N[C@@H](CC=4C=CC=CC=4)C(=O)N[C@@H](CC=4C=CC(O)=CC=4)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC=4C=CC=CC=4)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CCCCN)NC(=O)[C@H](C)NC(=O)[C@H](CCCNC(N)=N)NC2=O)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](CC=2C=CC=CC=2)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H]2N(CCC2)C(=O)[C@@H](N)CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N2[C@@H](CCC2)C(=O)N2[C@@H](CCC2)C(=O)N[C@@H](CC=2C=CC(O)=CC=2)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N2[C@@H](CCC2)C(=O)N3)C(=O)NCC(=O)NCC(=O)N[C@@H](C)C(O)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@H](C(=O)N[C@@H](CC=2C=CC=CC=2)C(=O)N[C@H](C(=O)N1)C(C)C)[C@@H](C)O)[C@@H](C)CC)=O)[C@@H](C)CC)C1=CC=C(O)C=C1 ZPNFWUPYTFPOJU-LPYSRVMUSA-N 0.000 description 3
• 239000007924 injection Substances 0.000 description 3
• 238000002347 injection Methods 0.000 description 3
• 238000003780 insertion Methods 0.000 description 3
• 230000037431 insertion Effects 0.000 description 3
• 150000002500 ions Chemical class 0.000 description 3
• GDBQQVLCIARPGH-ULQDDVLXSA-N leupeptin Chemical compound CC(C)C[C@H](NC(C)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](C=O)CCCN=C(N)N GDBQQVLCIARPGH-ULQDDVLXSA-N 0.000 description 3
• 108010052968 leupeptin Proteins 0.000 description 3
• 239000007788 liquid Substances 0.000 description 3
• 210000004937 luminal membrane Anatomy 0.000 description 3
• 238000012423 maintenance Methods 0.000 description 3
• 210000004962 mammalian cell Anatomy 0.000 description 3
• 239000000463 material Substances 0.000 description 3
• 230000035800 maturation Effects 0.000 description 3
• 210000003584 mesangial cell Anatomy 0.000 description 3
• 235000015097 nutrients Nutrition 0.000 description 3
• 230000036470 plasma concentration Effects 0.000 description 3
• 229920003023 plastic Polymers 0.000 description 3
• 239000004033 plastic Substances 0.000 description 3
• 229920002338 polyhydroxyethylmethacrylate Polymers 0.000 description 3
• 229920002981 polyvinylidene fluoride Polymers 0.000 description 3
• 230000026341 positive regulation of angiogenesis Effects 0.000 description 3
• 150000003141 primary amines Chemical group 0.000 description 3
• 230000007115 recruitment Effects 0.000 description 3
• 230000009467 reduction Effects 0.000 description 3
• 210000005084 renal tissue Anatomy 0.000 description 3
• 230000002207 retinal effect Effects 0.000 description 3
• 238000012552 review Methods 0.000 description 3
• PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 3
• 238000001179 sorption measurement Methods 0.000 description 3
• 238000001356 surgical procedure Methods 0.000 description 3
• 208000024891 symptom Diseases 0.000 description 3
• 238000003786 synthesis reaction Methods 0.000 description 3
• 238000011191 terminal modification Methods 0.000 description 3
• 125000005931 tert-butyloxycarbonyl group Chemical group [H]C([H])([H])C(OC(*)=O)(C([H])([H])[H])C([H])([H])[H] 0.000 description 3
• 231100000419 toxicity Toxicity 0.000 description 3
• 230000001988 toxicity Effects 0.000 description 3
• LSGOVYNHVSXFFJ-UHFFFAOYSA-N vanadate(3-) Chemical compound [O-][V]([O-])([O-])=O LSGOVYNHVSXFFJ-UHFFFAOYSA-N 0.000 description 3
• 230000003612 virological effect Effects 0.000 description 3
• MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 2
• VEEGZPWAAPPXRB-BJMVGYQFSA-N (3e)-3-(1h-imidazol-5-ylmethylidene)-1h-indol-2-one Chemical compound O=C1NC2=CC=CC=C2\C1=C/C1=CN=CN1 VEEGZPWAAPPXRB-BJMVGYQFSA-N 0.000 description 2
• HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 description 2
• HVBSAKJJOYLTQU-UHFFFAOYSA-N 4-aminobenzenesulfonic acid Chemical compound NC1=CC=C(S(O)(=O)=O)C=C1 HVBSAKJJOYLTQU-UHFFFAOYSA-N 0.000 description 2
• TVZGACDUOSZQKY-LBPRGKRZSA-N 4-aminofolic acid Chemical compound C1=NC2=NC(N)=NC(N)=C2N=C1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 TVZGACDUOSZQKY-LBPRGKRZSA-N 0.000 description 2
• STQGQHZAVUOBTE-UHFFFAOYSA-N 7-Cyan-hept-2t-en-4,6-diinsaeure Natural products C1=2C(O)=C3C(=O)C=4C(OC)=CC=CC=4C(=O)C3=C(O)C=2CC(O)(C(C)=O)CC1OC1CC(N)C(O)C(C)O1 STQGQHZAVUOBTE-UHFFFAOYSA-N 0.000 description 2
• QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
• 208000003120 Angiofibroma Diseases 0.000 description 2
• 102400000068 Angiostatin Human genes 0.000 description 2
• 108010079709 Angiostatins Proteins 0.000 description 2
• 241000272517 Anseriformes Species 0.000 description 2
• 108010032595 Antibody Binding Sites Proteins 0.000 description 2
• 239000004475 Arginine Substances 0.000 description 2
• 241000283690 Bos taurus Species 0.000 description 2
• 101710172562 Cobra venom factor Proteins 0.000 description 2
• 108020004635 Complementary DNA Proteins 0.000 description 2
• WEAHRLBPCANXCN-UHFFFAOYSA-N Daunomycin Natural products CCC1(O)CC(OC2CC(N)C(O)C(C)O2)c3cc4C(=O)c5c(OC)cccc5C(=O)c4c(O)c3C1 WEAHRLBPCANXCN-UHFFFAOYSA-N 0.000 description 2
• ROSDSFDQCJNGOL-UHFFFAOYSA-N Dimethylamine Chemical compound CNC ROSDSFDQCJNGOL-UHFFFAOYSA-N 0.000 description 2
• 101001102324 Drosophila melanogaster Tyrosine-protein phosphatase 10D Proteins 0.000 description 2
• 239000012983 Dulbecco’s minimal essential medium Substances 0.000 description 2
• 102400001047 Endostatin Human genes 0.000 description 2
• 108010079505 Endostatins Proteins 0.000 description 2
• 102400001368 Epidermal growth factor Human genes 0.000 description 2
• 101800003838 Epidermal growth factor Proteins 0.000 description 2
• 241000283086 Equidae Species 0.000 description 2
• 241000588724 Escherichia coli Species 0.000 description 2
• 108010074860 Factor Xa Proteins 0.000 description 2
• VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 description 2
• 208000010412 Glaucoma Diseases 0.000 description 2
• WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 2
• 108010024636 Glutathione Proteins 0.000 description 2
• DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
• AEMRFAOFKBGASW-UHFFFAOYSA-N Glycolic acid Chemical compound OCC(O)=O AEMRFAOFKBGASW-UHFFFAOYSA-N 0.000 description 2
• 239000007995 HEPES buffer Substances 0.000 description 2
• 241000238631 Hexapoda Species 0.000 description 2
• 108010001336 Horseradish Peroxidase Proteins 0.000 description 2
• DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
• XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 2
• ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 2
• CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 2
• 229930182816 L-glutamine Natural products 0.000 description 2
• OFOBLEOULBTSOW-UHFFFAOYSA-N Malonic acid Chemical compound OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 description 2
• 102000018697 Membrane Proteins Human genes 0.000 description 2
• 108010052285 Membrane Proteins Proteins 0.000 description 2
• NWIBSHFKIJFRCO-WUDYKRTCSA-N Mytomycin Chemical compound C1N2C(C(C(C)=C(N)C3=O)=O)=C3[C@@H](COC(N)=O)[C@@]2(OC)[C@@H]2[C@H]1N2 NWIBSHFKIJFRCO-WUDYKRTCSA-N 0.000 description 2
• 229910019142 PO4 Inorganic materials 0.000 description 2
• 229930182555 Penicillin Natural products 0.000 description 2
• JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 2
• 108091005804 Peptidases Proteins 0.000 description 2
• 102000035195 Peptidases Human genes 0.000 description 2
• 241000276498 Pollachius virens Species 0.000 description 2
• 239000002202 Polyethylene glycol Substances 0.000 description 2
• 229920001213 Polysorbate 20 Polymers 0.000 description 2
• 101000762949 Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) Exotoxin A Proteins 0.000 description 2
• LCTONWCANYUPML-UHFFFAOYSA-N Pyruvic acid Chemical compound CC(=O)C(O)=O LCTONWCANYUPML-UHFFFAOYSA-N 0.000 description 2
• 102100028516 Receptor-type tyrosine-protein phosphatase U Human genes 0.000 description 2
• 108010083644 Ribonucleases Proteins 0.000 description 2
• 102000006382 Ribonucleases Human genes 0.000 description 2
• 108010039491 Ricin Proteins 0.000 description 2
• 241000282849 Ruminantia Species 0.000 description 2
• 230000018199 S phase Effects 0.000 description 2
• MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 2
• CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
• 241000256251 Spodoptera frugiperda Species 0.000 description 2
• 108010090804 Streptavidin Proteins 0.000 description 2
• QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
• 108090000190 Thrombin Proteins 0.000 description 2
• 102000002938 Thrombospondin Human genes 0.000 description 2
• 108060008245 Thrombospondin Proteins 0.000 description 2
• 241000723873 Tobacco mosaic virus Species 0.000 description 2
• 239000007983 Tris buffer Substances 0.000 description 2
• 241000209140 Triticum Species 0.000 description 2
• 235000021307 Triticum Nutrition 0.000 description 2
• 108091008605 VEGF receptors Proteins 0.000 description 2
• 102000009484 Vascular Endothelial Growth Factor Receptors Human genes 0.000 description 2
• 230000002378 acidificating effect Effects 0.000 description 2
• 230000004913 activation Effects 0.000 description 2
• 230000002411 adverse Effects 0.000 description 2
• 230000004075 alteration Effects 0.000 description 2
• 229960003896 aminopterin Drugs 0.000 description 2
• 235000019270 ammonium chloride Nutrition 0.000 description 2
• RWZYAGGXGHYGMB-UHFFFAOYSA-N anthranilic acid Chemical compound NC1=CC=CC=C1C(O)=O RWZYAGGXGHYGMB-UHFFFAOYSA-N 0.000 description 2
• 230000000340 anti-metabolite Effects 0.000 description 2
• 229940100197 antimetabolite Drugs 0.000 description 2
• 239000002256 antimetabolite Substances 0.000 description 2
• ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 2
• 210000004618 arterial endothelial cell Anatomy 0.000 description 2
• 235000003704 aspartic acid Nutrition 0.000 description 2
• FZCSTZYAHCUGEM-UHFFFAOYSA-N aspergillomarasmine B Natural products OC(=O)CNC(C(O)=O)CNC(C(O)=O)CC(O)=O FZCSTZYAHCUGEM-UHFFFAOYSA-N 0.000 description 2
• 102000005936 beta-Galactosidase Human genes 0.000 description 2
• 108010005774 beta-Galactosidase Proteins 0.000 description 2
• OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 2
• 238000010256 biochemical assay Methods 0.000 description 2
• 230000031018 biological processes and functions Effects 0.000 description 2
• 230000033228 biological regulation Effects 0.000 description 2
• 210000004899 c-terminal region Anatomy 0.000 description 2
• 238000000423 cell based assay Methods 0.000 description 2
• 238000001516 cell proliferation assay Methods 0.000 description 2
• 238000005119 centrifugation Methods 0.000 description 2
• 230000008859 change Effects 0.000 description 2
• 230000009920 chelation Effects 0.000 description 2
• 238000002512 chemotherapy Methods 0.000 description 2
• 210000003837 chick embryo Anatomy 0.000 description 2
• YKCWQPZFAFZLBI-UHFFFAOYSA-N cibacron blue Chemical compound C1=2C(=O)C3=CC=CC=C3C(=O)C=2C(N)=C(S(O)(=O)=O)C=C1NC(C=C1S(O)(=O)=O)=CC=C1NC(N=1)=NC(Cl)=NC=1NC1=CC=CC=C1S(O)(=O)=O YKCWQPZFAFZLBI-UHFFFAOYSA-N 0.000 description 2
• 238000003776 cleavage reaction Methods 0.000 description 2
• 238000010367 cloning Methods 0.000 description 2
• 210000004246 corpus luteum Anatomy 0.000 description 2
• 210000004748 cultured cell Anatomy 0.000 description 2
• XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 2
• 235000018417 cysteine Nutrition 0.000 description 2
• 230000006378 damage Effects 0.000 description 2
• STQGQHZAVUOBTE-VGBVRHCVSA-N daunorubicin Chemical compound O([C@H]1C[C@@](O)(CC=2C(O)=C3C(=O)C=4C=CC=C(C=4C(=O)C3=C(O)C=21)OC)C(C)=O)[C@H]1C[C@H](N)[C@H](O)[C@H](C)O1 STQGQHZAVUOBTE-VGBVRHCVSA-N 0.000 description 2
• 230000007423 decrease Effects 0.000 description 2
• 230000002939 deleterious effect Effects 0.000 description 2
• 230000001419 dependent effect Effects 0.000 description 2
• 238000013461 design Methods 0.000 description 2
• 239000008121 dextrose Substances 0.000 description 2
• XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 2
• 238000010494 dissociation reaction Methods 0.000 description 2
• 230000005593 dissociations Effects 0.000 description 2
• 229960004679 doxorubicin Drugs 0.000 description 2
• 238000009510 drug design Methods 0.000 description 2
• 238000007877 drug screening Methods 0.000 description 2
• 230000013020 embryo development Effects 0.000 description 2
• 210000003989 endothelium vascular Anatomy 0.000 description 2
• 229940116977 epidermal growth factor Drugs 0.000 description 2
• 210000003560 epithelium corneal Anatomy 0.000 description 2
• 230000000925 erythroid effect Effects 0.000 description 2
• 210000003013 erythroid precursor cell Anatomy 0.000 description 2
• 235000013861 fat-free Nutrition 0.000 description 2
• 238000000684 flow cytometry Methods 0.000 description 2
• GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 2
• 125000000524 functional group Chemical group 0.000 description 2
• 235000013922 glutamic acid Nutrition 0.000 description 2
• ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 2
• 229960003180 glutathione Drugs 0.000 description 2
• 235000011187 glycerol Nutrition 0.000 description 2
• 230000009036 growth inhibition Effects 0.000 description 2
• 201000011066 hemangioma Diseases 0.000 description 2
• 230000003054 hormonal effect Effects 0.000 description 2
• 235000011167 hydrochloric acid Nutrition 0.000 description 2
• 229960000890 hydrocortisone Drugs 0.000 description 2
• 210000000987 immune system Anatomy 0.000 description 2
• 238000003125 immunofluorescent labeling Methods 0.000 description 2
• 230000002998 immunogenetic effect Effects 0.000 description 2
• 238000003364 immunohistochemistry Methods 0.000 description 2
• 239000012133 immunoprecipitate Substances 0.000 description 2
• 238000012613 in situ experiment Methods 0.000 description 2
• 238000000099 in vitro assay Methods 0.000 description 2
• 230000001939 inductive effect Effects 0.000 description 2
• 230000036512 infertility Effects 0.000 description 2
• 238000001802 infusion Methods 0.000 description 2
• 150000007529 inorganic bases Chemical class 0.000 description 2
• 230000003834 intracellular effect Effects 0.000 description 2
• 238000007912 intraperitoneal administration Methods 0.000 description 2
• 238000001990 intravenous administration Methods 0.000 description 2
• 230000009545 invasion Effects 0.000 description 2
• 238000012332 laboratory investigation Methods 0.000 description 2
• JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
• 210000000265 leukocyte Anatomy 0.000 description 2
• 238000012417 linear regression Methods 0.000 description 2
• 239000007791 liquid phase Substances 0.000 description 2
• 239000012139 lysis buffer Substances 0.000 description 2
• 239000003550 marker Substances 0.000 description 2
• 230000007246 mechanism Effects 0.000 description 2
• 108020004084 membrane receptors Proteins 0.000 description 2
• 108020004999 messenger RNA Proteins 0.000 description 2
• TWXDDNPPQUTEOV-FVGYRXGTSA-N methamphetamine hydrochloride Chemical compound Cl.CN[C@@H](C)CC1=CC=CC=C1 TWXDDNPPQUTEOV-FVGYRXGTSA-N 0.000 description 2
• 150000004702 methyl esters Chemical class 0.000 description 2
• 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 2
• 244000005700 microbiome Species 0.000 description 2
• 238000010232 migration assay Methods 0.000 description 2
• 235000013336 milk Nutrition 0.000 description 2
• 239000008267 milk Substances 0.000 description 2
• 210000004080 milk Anatomy 0.000 description 2
• 230000003278 mimic effect Effects 0.000 description 2
• 150000007522 mineralic acids Chemical class 0.000 description 2
• 230000009149 molecular binding Effects 0.000 description 2
• 238000010369 molecular cloning Methods 0.000 description 2
• 210000003205 muscle Anatomy 0.000 description 2
• 201000003142 neovascular glaucoma Diseases 0.000 description 2
• 230000003472 neutralizing effect Effects 0.000 description 2
• QYSGYZVSCZSLHT-UHFFFAOYSA-N octafluoropropane Chemical compound FC(F)(F)C(F)(F)C(F)(F)F QYSGYZVSCZSLHT-UHFFFAOYSA-N 0.000 description 2
• 150000007530 organic bases Chemical class 0.000 description 2
• 230000001575 pathological effect Effects 0.000 description 2
• 229940049954 penicillin Drugs 0.000 description 2
• VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical compound OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 description 2
• 239000002831 pharmacologic agent Substances 0.000 description 2
• 230000000144 pharmacologic effect Effects 0.000 description 2
• CTRLRINCMYICJO-UHFFFAOYSA-N phenyl azide Chemical class [N-]=[N+]=NC1=CC=CC=C1 CTRLRINCMYICJO-UHFFFAOYSA-N 0.000 description 2
• PHEDXBVPIONUQT-RGYGYFBISA-N phorbol 13-acetate 12-myristate Chemical compound C([C@]1(O)C(=O)C(C)=C[C@H]1[C@@]1(O)[C@H](C)[C@H]2OC(=O)CCCCCCCCCCCCC)C(CO)=C[C@H]1[C@H]1[C@]2(OC(C)=O)C1(C)C PHEDXBVPIONUQT-RGYGYFBISA-N 0.000 description 2
• NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
• 239000010452 phosphate Substances 0.000 description 2
• 210000000557 podocyte Anatomy 0.000 description 2
• 229920002401 polyacrylamide Polymers 0.000 description 2
• 229920001223 polyethylene glycol Polymers 0.000 description 2
• 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 2
• 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 2
• 244000144977 poultry Species 0.000 description 2
• 235000013594 poultry meat Nutrition 0.000 description 2
• 230000002062 proliferating effect Effects 0.000 description 2
• 230000006337 proteolytic cleavage Effects 0.000 description 2
• 239000002510 pyrogen Substances 0.000 description 2
• 108091008598 receptor tyrosine kinases Proteins 0.000 description 2
• 102000027426 receptor tyrosine kinases Human genes 0.000 description 2
• 230000006798 recombination Effects 0.000 description 2
• 238000005215 recombination Methods 0.000 description 2
• 230000021281 regulation of endothelial cell migration Effects 0.000 description 2
• 230000010045 regulation of endothelial cell proliferation Effects 0.000 description 2
• 102000037983 regulatory factors Human genes 0.000 description 2
• 108091008025 regulatory factors Proteins 0.000 description 2
• 238000007363 ring formation reaction Methods 0.000 description 2
• 230000007017 scission Effects 0.000 description 2
• 239000012679 serum free medium Substances 0.000 description 2
• 210000003491 skin Anatomy 0.000 description 2
• 229910052708 sodium Inorganic materials 0.000 description 2
• 239000011734 sodium Substances 0.000 description 2
• 239000011780 sodium chloride Substances 0.000 description 2
• 238000010532 solid phase synthesis reaction Methods 0.000 description 2
• 230000000087 stabilizing effect Effects 0.000 description 2
• 229960005322 streptomycin Drugs 0.000 description 2
• 238000010257 thawing Methods 0.000 description 2
• 229940124597 therapeutic agent Drugs 0.000 description 2
• 229940126585 therapeutic drug Drugs 0.000 description 2
• ZMZDMBWJUHKJPS-UHFFFAOYSA-N thiocyanic acid Chemical compound SC#N ZMZDMBWJUHKJPS-UHFFFAOYSA-N 0.000 description 2
• CWERGRDVMFNCDR-UHFFFAOYSA-N thioglycolic acid Chemical compound OC(=O)CS CWERGRDVMFNCDR-UHFFFAOYSA-N 0.000 description 2
• 125000003396 thiol group Chemical group [H]S* 0.000 description 2
• 229960004072 thrombin Drugs 0.000 description 2
• 231100000331 toxic Toxicity 0.000 description 2
• 230000002588 toxic effect Effects 0.000 description 2
• 230000007888 toxin activity Effects 0.000 description 2
• 238000010361 transduction Methods 0.000 description 2
• 230000026683 transduction Effects 0.000 description 2
• 238000011830 transgenic mouse model Methods 0.000 description 2
• GETQZCLCWQTVFV-UHFFFAOYSA-N trimethylamine Chemical compound CN(C)C GETQZCLCWQTVFV-UHFFFAOYSA-N 0.000 description 2
• LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
• 239000003656 tris buffered saline Substances 0.000 description 2
• IHIXIJGXTJIKRB-UHFFFAOYSA-N trisodium vanadate Chemical compound [Na+].[Na+].[Na+].[O-][V]([O-])([O-])=O IHIXIJGXTJIKRB-UHFFFAOYSA-N 0.000 description 2
• VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 description 2
• 230000029663 wound healing Effects 0.000 description 2
• 238000001086 yeast two-hybrid system Methods 0.000 description 2
• NNJPGOLRFBJNIW-HNNXBMFYSA-N (-)-demecolcine Chemical compound C1=C(OC)C(=O)C=C2[C@@H](NC)CCC3=CC(OC)=C(OC)C(OC)=C3C2=C1 NNJPGOLRFBJNIW-HNNXBMFYSA-N 0.000 description 1
• QGVLYPPODPLXMB-UBTYZVCOSA-N (1aR,1bS,4aR,7aS,7bS,8R,9R,9aS)-4a,7b,9,9a-tetrahydroxy-3-(hydroxymethyl)-1,1,6,8-tetramethyl-1,1a,1b,4,4a,7a,7b,8,9,9a-decahydro-5H-cyclopropa[3,4]benzo[1,2-e]azulen-5-one Chemical compound C1=C(CO)C[C@]2(O)C(=O)C(C)=C[C@H]2[C@@]2(O)[C@H](C)[C@@H](O)[C@@]3(O)C(C)(C)[C@H]3[C@@H]21 QGVLYPPODPLXMB-UBTYZVCOSA-N 0.000 description 1
• JWDFQMWEFLOOED-UHFFFAOYSA-N (2,5-dioxopyrrolidin-1-yl) 3-(pyridin-2-yldisulfanyl)propanoate Chemical compound O=C1CCC(=O)N1OC(=O)CCSSC1=CC=CC=N1 JWDFQMWEFLOOED-UHFFFAOYSA-N 0.000 description 1
• DIGQNXIGRZPYDK-WKSCXVIASA-N (2R)-6-amino-2-[[2-[[(2S)-2-[[2-[[(2R)-2-[[(2S)-2-[[(2R,3S)-2-[[2-[[(2S)-2-[[2-[[(2S)-2-[[(2S)-2-[[(2R)-2-[[(2S,3S)-2-[[(2R)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[2-[[(2S)-2-[[(2R)-2-[[2-[[2-[[2-[(2-amino-1-hydroxyethylidene)amino]-3-carboxy-1-hydroxypropylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxybutylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1,5-dihydroxy-5-iminopentylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxybutylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxyethylidene]amino]hexanoic acid Chemical compound C[C@@H]([C@@H](C(=N[C@@H](CS)C(=N[C@@H](C)C(=N[C@@H](CO)C(=NCC(=N[C@@H](CCC(=N)O)C(=NC(CS)C(=N[C@H]([C@H](C)O)C(=N[C@H](CS)C(=N[C@H](CO)C(=NCC(=N[C@H](CS)C(=NCC(=N[C@H](CCCCN)C(=O)O)O)O)O)O)O)O)O)O)O)O)O)O)O)N=C([C@H](CS)N=C([C@H](CO)N=C([C@H](CO)N=C([C@H](C)N=C(CN=C([C@H](CO)N=C([C@H](CS)N=C(CN=C(C(CS)N=C(C(CC(=O)O)N=C(CN)O)O)O)O)O)O)O)O)O)O)O)O DIGQNXIGRZPYDK-WKSCXVIASA-N 0.000 description 1
• 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
• UKAUYVFTDYCKQA-UHFFFAOYSA-N -2-Amino-4-hydroxybutanoic acid Natural products OC(=O)C(N)CCO UKAUYVFTDYCKQA-UHFFFAOYSA-N 0.000 description 1
• WBYWAXJHAXSJNI-VOTSOKGWSA-M .beta-Phenylacrylic acid Natural products [O-]C(=O)\C=C\C1=CC=CC=C1 WBYWAXJHAXSJNI-VOTSOKGWSA-M 0.000 description 1
• RYHBNJHYFVUHQT-UHFFFAOYSA-N 1,4-Dioxane Chemical compound C1COCCO1 RYHBNJHYFVUHQT-UHFFFAOYSA-N 0.000 description 1
• ASOKPJOREAFHNY-UHFFFAOYSA-N 1-Hydroxybenzotriazole Chemical compound C1=CC=C2N(O)N=NC2=C1 ASOKPJOREAFHNY-UHFFFAOYSA-N 0.000 description 1
• GHKCSRZBNZQHKW-UHFFFAOYSA-N 1-sulfanylethanol Chemical compound CC(O)S GHKCSRZBNZQHKW-UHFFFAOYSA-N 0.000 description 1
• 150000003923 2,5-pyrrolediones Chemical class 0.000 description 1
• MIJDSYMOBYNHOT-UHFFFAOYSA-N 2-(ethylamino)ethanol Chemical compound CCNCCO MIJDSYMOBYNHOT-UHFFFAOYSA-N 0.000 description 1
• BFFPVEVGHKMWLT-UHFFFAOYSA-N 2-amino-3,7-dihydropurin-6-one;3,7-dihydropurin-6-one Chemical compound O=C1NC=NC2=C1NC=N2.O=C1NC(N)=NC2=C1NC=N2 BFFPVEVGHKMWLT-UHFFFAOYSA-N 0.000 description 1
• OZDAOHVKBFBBMZ-UHFFFAOYSA-N 2-aminopentanedioic acid;hydrate Chemical compound O.OC(=O)C(N)CCC(O)=O OZDAOHVKBFBBMZ-UHFFFAOYSA-N 0.000 description 1
• HSTOKWSFWGCZMH-UHFFFAOYSA-N 3,3'-diaminobenzidine Chemical compound C1=C(N)C(N)=CC=C1C1=CC=C(N)C(N)=C1 HSTOKWSFWGCZMH-UHFFFAOYSA-N 0.000 description 1
• BRMWTNUJHUMWMS-UHFFFAOYSA-N 3-Methylhistidine Natural products CN1C=NC(CC(N)C(O)=O)=C1 BRMWTNUJHUMWMS-UHFFFAOYSA-N 0.000 description 1
• BMYNFMYTOJXKLE-UHFFFAOYSA-N 3-azaniumyl-2-hydroxypropanoate Chemical compound NCC(O)C(O)=O BMYNFMYTOJXKLE-UHFFFAOYSA-N 0.000 description 1
• 229940117976 5-hydroxylysine Drugs 0.000 description 1
• QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
• 108010024223 Adenine phosphoribosyltransferase Proteins 0.000 description 1
• QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
• VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
• 229940122659 Angiogenesis stimulant Drugs 0.000 description 1
• 108091023037 Aptamer Proteins 0.000 description 1
• DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
• 101000669426 Aspergillus restrictus Ribonuclease mitogillin Proteins 0.000 description 1
• 241000182988 Assa Species 0.000 description 1
• 208000037260 Atherosclerotic Plaque Diseases 0.000 description 1
• 235000014469 Bacillus subtilis Nutrition 0.000 description 1
• 241000283726 Bison Species 0.000 description 1
• 241000283725 Bos Species 0.000 description 1
• 238000012756 BrdU staining Methods 0.000 description 1
• 206010006187 Breast cancer Diseases 0.000 description 1
• 208000026310 Breast neoplasm Diseases 0.000 description 1
• 101100043372 Caenorhabditis elegans sqt-1 gene Proteins 0.000 description 1
• 241000282832 Camelidae Species 0.000 description 1
• 241000282472 Canis lupus familiaris Species 0.000 description 1
• 201000009030 Carcinoma Diseases 0.000 description 1
• 206010007559 Cardiac failure congestive Diseases 0.000 description 1
• 241001466804 Carnivora Species 0.000 description 1
• 241000701489 Cauliflower mosaic virus Species 0.000 description 1
• 241000282994 Cervidae Species 0.000 description 1
• WBYWAXJHAXSJNI-SREVYHEPSA-N Cinnamic acid Chemical compound OC(=O)\C=C/C1=CC=CC=C1 WBYWAXJHAXSJNI-SREVYHEPSA-N 0.000 description 1
• 102000007644 Colony-Stimulating Factors Human genes 0.000 description 1
• 108010071942 Colony-Stimulating Factors Proteins 0.000 description 1
• 241000699802 Cricetulus griseus Species 0.000 description 1
• UHDGCWIWMRVCDJ-CCXZUQQUSA-N Cytarabine Chemical compound O=C1N=C(N)C=CN1[C@H]1[C@@H](O)[C@H](O)[C@@H](CO)O1 UHDGCWIWMRVCDJ-CCXZUQQUSA-N 0.000 description 1
• 101150074155 DHFR gene Proteins 0.000 description 1
• 239000003155 DNA primer Substances 0.000 description 1
• 230000004543 DNA replication Effects 0.000 description 1
• 230000006820 DNA synthesis Effects 0.000 description 1
• 241000271032 Daboia russelii Species 0.000 description 1
• NNJPGOLRFBJNIW-UHFFFAOYSA-N Demecolcine Natural products C1=C(OC)C(=O)C=C2C(NC)CCC3=CC(OC)=C(OC)C(OC)=C3C2=C1 NNJPGOLRFBJNIW-UHFFFAOYSA-N 0.000 description 1
• QOSSAOTZNIDXMA-UHFFFAOYSA-N Dicylcohexylcarbodiimide Chemical compound C1CCCCC1N=C=NC1CCCCC1 QOSSAOTZNIDXMA-UHFFFAOYSA-N 0.000 description 1
• BWGNESOTFCXPMA-UHFFFAOYSA-N Dihydrogen disulfide Chemical compound SS BWGNESOTFCXPMA-UHFFFAOYSA-N 0.000 description 1
• 102000016607 Diphtheria Toxin Human genes 0.000 description 1
• 108010053187 Diphtheria Toxin Proteins 0.000 description 1
• 101000738734 Drosophila melanogaster Tyrosine-protein phosphatase 69D Proteins 0.000 description 1
• 101710130332 ETS domain-containing protein Elk-4 Proteins 0.000 description 1
• 241000196324 Embryophyta Species 0.000 description 1
• 102000050554 Eph Family Receptors Human genes 0.000 description 1
• 108091008815 Eph receptors Proteins 0.000 description 1
• 108010055336 EphB1 Receptor Proteins 0.000 description 1
• YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 1
• VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
• 239000005977 Ethylene Substances 0.000 description 1
• 108010048049 Factor IXa Proteins 0.000 description 1
• 241000282326 Felis catus Species 0.000 description 1
• 102100024785 Fibroblast growth factor 2 Human genes 0.000 description 1
• 108090000379 Fibroblast growth factor 2 Proteins 0.000 description 1
• 102000002090 Fibronectin type III Human genes 0.000 description 1
• 108050009401 Fibronectin type III Proteins 0.000 description 1
• 241000287828 Gallus gallus Species 0.000 description 1
• 108700004714 Gelonium multiflorum GEL Proteins 0.000 description 1
• 108700028146 Genetic Enhancer Elements Proteins 0.000 description 1
• 241000282818 Giraffidae Species 0.000 description 1
• 239000004471 Glycine Substances 0.000 description 1
• 108010043121 Green Fluorescent Proteins Proteins 0.000 description 1
• 102000004144 Green Fluorescent Proteins Human genes 0.000 description 1
• 206010019280 Heart failures Diseases 0.000 description 1
• 101100321817 Human parvovirus B19 (strain HV) 7.5K gene Proteins 0.000 description 1
• PMMYEEVYMWASQN-DMTCNVIQSA-N Hydroxyproline Chemical compound O[C@H]1CN[C@H](C(O)=O)C1 PMMYEEVYMWASQN-DMTCNVIQSA-N 0.000 description 1
• 206010051151 Hyperviscosity syndrome Diseases 0.000 description 1
• 206010061218 Inflammation Diseases 0.000 description 1
• 108020005350 Initiator Codon Proteins 0.000 description 1
• 102000015696 Interleukins Human genes 0.000 description 1
• 108010063738 Interleukins Proteins 0.000 description 1
• AHLPHDHHMVZTML-BYPYZUCNSA-N L-Ornithine Chemical compound NCCC[C@H](N)C(O)=O AHLPHDHHMVZTML-BYPYZUCNSA-N 0.000 description 1
• DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 1
• WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
• UKAUYVFTDYCKQA-VKHMYHEASA-N L-homoserine Chemical compound OC(=O)[C@@H](N)CCO UKAUYVFTDYCKQA-VKHMYHEASA-N 0.000 description 1
• AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 1
• ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
• FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
• AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 1
• KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 1
• ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
• 102000003792 Metallothionein Human genes 0.000 description 1
• 108090000157 Metallothionein Proteins 0.000 description 1
• JDHILDINMRGULE-LURJTMIESA-N N(pros)-methyl-L-histidine Chemical compound CN1C=NC=C1C[C@H](N)C(O)=O JDHILDINMRGULE-LURJTMIESA-N 0.000 description 1
• QPCDCPDFJACHGM-UHFFFAOYSA-N N,N-bis{2-[bis(carboxymethyl)amino]ethyl}glycine Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(=O)O)CCN(CC(O)=O)CC(O)=O QPCDCPDFJACHGM-UHFFFAOYSA-N 0.000 description 1
• NQTADLQHYWFPDB-UHFFFAOYSA-N N-Hydroxysuccinimide Chemical compound ON1C(=O)CCC1=O NQTADLQHYWFPDB-UHFFFAOYSA-N 0.000 description 1
• 230000004988 N-glycosylation Effects 0.000 description 1
• 206010028813 Nausea Diseases 0.000 description 1
• 101710204212 Neocarzinostatin Proteins 0.000 description 1
• GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 1
• 241000272458 Numididae Species 0.000 description 1
• 108700020796 Oncogene Proteins 0.000 description 1
• AHLPHDHHMVZTML-UHFFFAOYSA-N Orn-delta-NH2 Natural products NCCCC(N)C(O)=O AHLPHDHHMVZTML-UHFFFAOYSA-N 0.000 description 1
• UTJLXEIPEHZYQJ-UHFFFAOYSA-N Ornithine Natural products OC(=O)C(C)CCCN UTJLXEIPEHZYQJ-UHFFFAOYSA-N 0.000 description 1
• 208000001132 Osteoporosis Diseases 0.000 description 1
• 102000016979 Other receptors Human genes 0.000 description 1
• 241001278385 Panthera tigris altaica Species 0.000 description 1
• 229930040373 Paraformaldehyde Natural products 0.000 description 1
• 241001494479 Pecora Species 0.000 description 1
• 108010067902 Peptide Library Proteins 0.000 description 1
• 241000286209 Phasianidae Species 0.000 description 1
• 108700019535 Phosphoprotein Phosphatases Proteins 0.000 description 1
• 108010089430 Phosphoproteins Proteins 0.000 description 1
• 102000007982 Phosphoproteins Human genes 0.000 description 1
• 241000235648 Pichia Species 0.000 description 1
• 229920002535 Polyethylene Glycol 1500 Polymers 0.000 description 1
• ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
• WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical class [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 1
• ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
• 239000004365 Protease Substances 0.000 description 1
• 102000002727 Protein Tyrosine Phosphatase Human genes 0.000 description 1
• 201000004681 Psoriasis Diseases 0.000 description 1
• 206010037423 Pulmonary oedema Diseases 0.000 description 1
• 230000006819 RNA synthesis Effects 0.000 description 1
• 108010025497 Raytide Proteins 0.000 description 1
• 102100039664 Receptor-type tyrosine-protein phosphatase H Human genes 0.000 description 1
• 101710138742 Receptor-type tyrosine-protein phosphatase H Proteins 0.000 description 1
• 206010038933 Retinopathy of prematurity Diseases 0.000 description 1
• 108090000829 Ribosome Inactivating Proteins Proteins 0.000 description 1
• 241000235070 Saccharomyces Species 0.000 description 1
• 108010084592 Saporins Proteins 0.000 description 1
• 206010039491 Sarcoma Diseases 0.000 description 1
• 241000700584 Simplexvirus Species 0.000 description 1
• 208000000453 Skin Neoplasms Diseases 0.000 description 1
• VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
• KDYFGRWQOYBRFD-UHFFFAOYSA-N Succinic acid Natural products OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 description 1
• 102000006601 Thymidine Kinase Human genes 0.000 description 1
• 108020004440 Thymidine kinase Proteins 0.000 description 1
• YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 1
• 101710182532 Toxin a Proteins 0.000 description 1
• 102000014384 Type C Phospholipases Human genes 0.000 description 1
• 108010079194 Type C Phospholipases Proteins 0.000 description 1
• 229940076850 Tyrosine phosphatase inhibitor Drugs 0.000 description 1
• 241000700618 Vaccinia virus Species 0.000 description 1
• KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 1
• JXLYSJRDGCGARV-WWYNWVTFSA-N Vinblastine Natural products O=C(O[C@H]1[C@](O)(C(=O)OC)[C@@H]2N(C)c3c(cc(c(OC)c3)[C@]3(C(=O)OC)c4[nH]c5c(c4CCN4C[C@](O)(CC)C[C@H](C3)C4)cccc5)[C@@]32[C@H]2[C@@]1(CC)C=CCN2CC3)C JXLYSJRDGCGARV-WWYNWVTFSA-N 0.000 description 1
• 229940122803 Vinca alkaloid Drugs 0.000 description 1
• 108010046516 Wheat Germ Agglutinins Proteins 0.000 description 1
• JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
• GELXFVQAWNTGPQ-UHFFFAOYSA-N [N].C1=CNC=N1 Chemical compound [N].C1=CNC=N1 GELXFVQAWNTGPQ-UHFFFAOYSA-N 0.000 description 1
• 230000002159 abnormal effect Effects 0.000 description 1
• 238000002835 absorbance Methods 0.000 description 1
• 230000021736 acetylation Effects 0.000 description 1
• 238000006640 acetylation reaction Methods 0.000 description 1
• 239000012190 activator Substances 0.000 description 1
• 239000011149 active material Substances 0.000 description 1
• 230000001154 acute effect Effects 0.000 description 1
• 230000010933 acylation Effects 0.000 description 1
• 238000005917 acylation reaction Methods 0.000 description 1
• 239000000654 additive Substances 0.000 description 1
• 239000000556 agonist Substances 0.000 description 1
• HAXFWIACAGNFHA-UHFFFAOYSA-N aldrithiol Chemical compound C=1C=CC=NC=1SSC1=CC=CC=N1 HAXFWIACAGNFHA-UHFFFAOYSA-N 0.000 description 1
• 239000002168 alkylating agent Substances 0.000 description 1
• 229940100198 alkylating agent Drugs 0.000 description 1
• 230000002152 alkylating effect Effects 0.000 description 1
• 230000001668 ameliorated effect Effects 0.000 description 1
• 230000009435 amidation Effects 0.000 description 1
• 238000007112 amidation reaction Methods 0.000 description 1
• 150000003862 amino acid derivatives Chemical class 0.000 description 1
• 229940126575 aminoglycoside Drugs 0.000 description 1
• 229910021529 ammonia Inorganic materials 0.000 description 1
• 239000000908 ammonium hydroxide Substances 0.000 description 1
• 230000019552 anatomical structure morphogenesis Effects 0.000 description 1
• 210000000648 angioblast Anatomy 0.000 description 1
• 239000002870 angiogenesis inducing agent Substances 0.000 description 1
• 230000000964 angiostatic effect Effects 0.000 description 1
• 230000003042 antagnostic effect Effects 0.000 description 1
• 229940045799 anthracyclines and related substance Drugs 0.000 description 1
• 230000001772 anti-angiogenic effect Effects 0.000 description 1
• 230000002022 anti-cellular effect Effects 0.000 description 1
• 230000003466 anti-cipated effect Effects 0.000 description 1
• 230000002482 anti-endothelial effect Effects 0.000 description 1
• 230000002785 anti-thrombosis Effects 0.000 description 1
• 210000000628 antibody-producing cell Anatomy 0.000 description 1
• 239000003146 anticoagulant agent Substances 0.000 description 1
• 230000000890 antigenic effect Effects 0.000 description 1
• 229940034982 antineoplastic agent Drugs 0.000 description 1
• 229940041181 antineoplastic drug Drugs 0.000 description 1
• 239000008365 aqueous carrier Substances 0.000 description 1
• 239000003125 aqueous solvent Substances 0.000 description 1
• 150000004982 aromatic amines Chemical class 0.000 description 1
• 230000002917 arthritic effect Effects 0.000 description 1
• 238000013528 artificial neural network Methods 0.000 description 1
• 235000009582 asparagine Nutrition 0.000 description 1
• 229960001230 asparagine Drugs 0.000 description 1
• FIVPIPIDMRVLAY-UHFFFAOYSA-N aspergillin Natural products C1C2=CC=CC(O)C2N2C1(SS1)C(=O)N(C)C1(CO)C2=O FIVPIPIDMRVLAY-UHFFFAOYSA-N 0.000 description 1
• 239000012131 assay buffer Substances 0.000 description 1
• 238000000376 autoradiography Methods 0.000 description 1
• 230000003376 axonal effect Effects 0.000 description 1
• 230000001580 bacterial effect Effects 0.000 description 1
• 239000011324 bead Substances 0.000 description 1
• 125000001584 benzyloxycarbonyl group Chemical group C(=O)(OCC1=CC=CC=C1)* 0.000 description 1
• WZZVUHWLNMNWLW-VFCSDQTKSA-N beta-D-GlcpNAc-(1->4)-beta-D-GlcpNAc-(1->4)-beta-D-GlcpNAc Chemical compound O[C@@H]1[C@@H](NC(=O)C)[C@H](O)O[C@H](CO)[C@H]1O[C@H]1[C@H](NC(C)=O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](CO)O1 WZZVUHWLNMNWLW-VFCSDQTKSA-N 0.000 description 1
• 230000001588 bifunctional effect Effects 0.000 description 1
• 102000023732 binding proteins Human genes 0.000 description 1
• 108091008324 binding proteins Proteins 0.000 description 1
• 239000013060 biological fluid Substances 0.000 description 1
• 230000008512 biological response Effects 0.000 description 1
• 229960002685 biotin Drugs 0.000 description 1
• 239000011616 biotin Substances 0.000 description 1
• 210000001772 blood platelet Anatomy 0.000 description 1
• 230000037396 body weight Effects 0.000 description 1
• 210000000988 bone and bone Anatomy 0.000 description 1
• 210000004556 brain Anatomy 0.000 description 1
• 210000000481 breast Anatomy 0.000 description 1
• 239000000337 buffer salt Substances 0.000 description 1
• KDYFGRWQOYBRFD-NUQCWPJISA-N butanedioic acid Chemical compound O[14C](=O)CC[14C](O)=O KDYFGRWQOYBRFD-NUQCWPJISA-N 0.000 description 1
• 239000006227 byproduct Substances 0.000 description 1
• 210000001043 capillary endothelial cell Anatomy 0.000 description 1
• 125000000837 carbohydrate group Chemical group 0.000 description 1
• 150000001720 carbohydrates Chemical class 0.000 description 1
• 235000014633 carbohydrates Nutrition 0.000 description 1
• RBHJBMIOOPYDBQ-UHFFFAOYSA-N carbon dioxide;propan-2-one Chemical compound O=C=O.CC(C)=O RBHJBMIOOPYDBQ-UHFFFAOYSA-N 0.000 description 1
• 125000002091 cationic group Chemical group 0.000 description 1
• 230000022131 cell cycle Effects 0.000 description 1
• 230000022534 cell killing Effects 0.000 description 1
• 230000005859 cell recognition Effects 0.000 description 1
• 230000033383 cell-cell recognition Effects 0.000 description 1
• 230000033077 cellular process Effects 0.000 description 1
• 238000012512 characterization method Methods 0.000 description 1
• 150000004697 chelate complex Chemical class 0.000 description 1
• 239000002738 chelating agent Substances 0.000 description 1
• 238000001311 chemical methods and process Methods 0.000 description 1
• 239000003638 chemical reducing agent Substances 0.000 description 1
• 230000000973 chemotherapeutic effect Effects 0.000 description 1
• 235000013330 chicken meat Nutrition 0.000 description 1
• 125000002668 chloroacetyl group Chemical group ClCC(=O)* 0.000 description 1
• 238000004587 chromatography analysis Methods 0.000 description 1
• 210000000349 chromosome Anatomy 0.000 description 1
• 235000013985 cinnamic acid Nutrition 0.000 description 1
• 229930016911 cinnamic acid Natural products 0.000 description 1
• 210000003040 circulating cell Anatomy 0.000 description 1
• 230000004186 co-expression Effects 0.000 description 1
• 239000000701 coagulant Substances 0.000 description 1
• 230000015271 coagulation Effects 0.000 description 1
• 238000005345 coagulation Methods 0.000 description 1
• 229940047120 colony stimulating factors Drugs 0.000 description 1
• 238000004891 communication Methods 0.000 description 1
• 210000002808 connective tissue Anatomy 0.000 description 1
• 238000010276 construction Methods 0.000 description 1
• 239000000356 contaminant Substances 0.000 description 1
• 238000011109 contamination Methods 0.000 description 1
• 238000011254 conventional chemotherapy Methods 0.000 description 1
• 210000004087 cornea Anatomy 0.000 description 1
• 230000001054 cortical effect Effects 0.000 description 1
• 239000006184 cosolvent Substances 0.000 description 1
• 235000012343 cottonseed oil Nutrition 0.000 description 1
• 239000002385 cottonseed oil Substances 0.000 description 1
• 239000007822 coupling agent Substances 0.000 description 1
• WZHCOOQXZCIUNC-UHFFFAOYSA-N cyclandelate Chemical compound C1C(C)(C)CC(C)CC1OC(=O)C(O)C1=CC=CC=C1 WZHCOOQXZCIUNC-UHFFFAOYSA-N 0.000 description 1
• 125000004122 cyclic group Chemical group 0.000 description 1
• 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 description 1
• 231100000433 cytotoxic Toxicity 0.000 description 1
• 239000002254 cytotoxic agent Substances 0.000 description 1
• 231100000599 cytotoxic agent Toxicity 0.000 description 1
• 230000001472 cytotoxic effect Effects 0.000 description 1
• 230000003247 decreasing effect Effects 0.000 description 1
• 230000002950 deficient Effects 0.000 description 1
• 230000006735 deficit Effects 0.000 description 1
• 230000003412 degenerative effect Effects 0.000 description 1
• YSMODUONRAFBET-UHFFFAOYSA-N delta-DL-hydroxylysine Natural products NCC(O)CCC(N)C(O)=O YSMODUONRAFBET-UHFFFAOYSA-N 0.000 description 1
• 229960005052 demecolcine Drugs 0.000 description 1
• CFCUWKMKBJTWLW-UHFFFAOYSA-N deoliosyl-3C-alpha-L-digitoxosyl-MTM Natural products CC=1C(O)=C2C(O)=C3C(=O)C(OC4OC(C)C(O)C(OC5OC(C)C(O)C(OC6OC(C)C(O)C(C)(O)C6)C5)C4)C(C(OC)C(=O)C(O)C(C)O)CC3=CC2=CC=1OC(OC(C)C1O)CC1OC1CC(O)C(O)C(C)O1 CFCUWKMKBJTWLW-UHFFFAOYSA-N 0.000 description 1
• 229940009976 deoxycholate Drugs 0.000 description 1
• KXGVEGMKQFWNSR-LLQZFEROSA-N deoxycholic acid Chemical compound C([C@H]1CC2)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 KXGVEGMKQFWNSR-LLQZFEROSA-N 0.000 description 1
• 230000000368 destabilizing effect Effects 0.000 description 1
• 239000003599 detergent Substances 0.000 description 1
• ZBCBWPMODOFKDW-UHFFFAOYSA-N diethanolamine Chemical compound OCCNCCO ZBCBWPMODOFKDW-UHFFFAOYSA-N 0.000 description 1
• 230000029087 digestion Effects 0.000 description 1
• 229940043279 diisopropylamine Drugs 0.000 description 1
• 238000010790 dilution Methods 0.000 description 1
• 239000012895 dilution Substances 0.000 description 1
• VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 1
• 208000002173 dizziness Diseases 0.000 description 1
• PMMYEEVYMWASQN-UHFFFAOYSA-N dl-hydroxyproline Natural products OC1C[NH2+]C(C([O-])=O)C1 PMMYEEVYMWASQN-UHFFFAOYSA-N 0.000 description 1
• 230000008030 elimination Effects 0.000 description 1
• 238000003379 elimination reaction Methods 0.000 description 1
• 230000008011 embryonic death Effects 0.000 description 1
• 210000002257 embryonic structure Anatomy 0.000 description 1
• 239000003995 emulsifying agent Substances 0.000 description 1
• 239000000839 emulsion Substances 0.000 description 1
• 210000004696 endometrium Anatomy 0.000 description 1
• 210000002472 endoplasmic reticulum Anatomy 0.000 description 1
• 239000003623 enhancer Substances 0.000 description 1
• 102000012803 ephrin Human genes 0.000 description 1
• 108060002566 ephrin Proteins 0.000 description 1
• 230000003628 erosive effect Effects 0.000 description 1
• YSMODUONRAFBET-UHNVWZDZSA-N erythro-5-hydroxy-L-lysine Chemical compound NC[C@H](O)CC[C@H](N)C(O)=O YSMODUONRAFBET-UHNVWZDZSA-N 0.000 description 1
• 150000002148 esters Chemical class 0.000 description 1
• 150000002169 ethanolamines Chemical class 0.000 description 1
• 125000004494 ethyl ester group Chemical group 0.000 description 1
• 229960005420 etoposide Drugs 0.000 description 1
• VJJPUSNTGOMMGY-MRVIYFEKSA-N etoposide Chemical compound COC1=C(O)C(OC)=CC([C@@H]2C3=CC=4OCOC=4C=C3[C@@H](O[C@H]3[C@@H]([C@@H](O)[C@@H]4O[C@H](C)OC[C@H]4O3)O)[C@@H]3[C@@H]2C(OC3)=O)=C1 VJJPUSNTGOMMGY-MRVIYFEKSA-N 0.000 description 1
• 210000003527 eukaryotic cell Anatomy 0.000 description 1
• 238000001704 evaporation Methods 0.000 description 1
• 230000007717 exclusion Effects 0.000 description 1
• 238000002270 exclusion chromatography Methods 0.000 description 1
• 210000002744 extracellular matrix Anatomy 0.000 description 1
• HJUFTIJOISQSKQ-UHFFFAOYSA-N fenoxycarb Chemical compound C1=CC(OCCNC(=O)OCC)=CC=C1OC1=CC=CC=C1 HJUFTIJOISQSKQ-UHFFFAOYSA-N 0.000 description 1
• 239000012894 fetal calf serum Substances 0.000 description 1
• 230000008175 fetal development Effects 0.000 description 1
• 230000001605 fetal effect Effects 0.000 description 1
• 238000013100 final test Methods 0.000 description 1
• MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 1
• 125000002485 formyl group Chemical group [H]C(*)=O 0.000 description 1
• 239000001530 fumaric acid Substances 0.000 description 1
• 230000002496 gastric effect Effects 0.000 description 1
• 210000001035 gastrointestinal tract Anatomy 0.000 description 1
• 238000010448 genetic screening Methods 0.000 description 1
• FIVPIPIDMRVLAY-RBJBARPLSA-N gliotoxin Chemical compound C1C2=CC=C[C@H](O)[C@H]2N2[C@]1(SS1)C(=O)N(C)[C@@]1(CO)C2=O FIVPIPIDMRVLAY-RBJBARPLSA-N 0.000 description 1
• 210000000585 glomerular basement membrane Anatomy 0.000 description 1
• 210000004244 glomerular capillary endothelial cell Anatomy 0.000 description 1
• 210000001707 glomerular endothelial cell Anatomy 0.000 description 1
• 230000028471 glomerulus development Effects 0.000 description 1
• 239000008103 glucose Substances 0.000 description 1
• 239000004220 glutamic acid Substances 0.000 description 1
• 239000003966 growth inhibitor Substances 0.000 description 1
• 229910052736 halogen Inorganic materials 0.000 description 1
• 150000002367 halogens Chemical class 0.000 description 1
• 238000003306 harvesting Methods 0.000 description 1
• 230000036541 health Effects 0.000 description 1
• 210000002216 heart Anatomy 0.000 description 1
• 210000003566 hemangioblast Anatomy 0.000 description 1
• 238000004128 high performance liquid chromatography Methods 0.000 description 1
• 238000013537 high throughput screening Methods 0.000 description 1
• 229920001519 homopolymer Polymers 0.000 description 1
• 229940088597 hormone Drugs 0.000 description 1
• 239000005556 hormone Substances 0.000 description 1
• 238000009396 hybridization Methods 0.000 description 1
• 229940042795 hydrazides for tuberculosis treatment Drugs 0.000 description 1
• OAKJQQAXSVQMHS-UHFFFAOYSA-N hydrazine group Chemical group NN OAKJQQAXSVQMHS-UHFFFAOYSA-N 0.000 description 1
• 230000002209 hydrophobic effect Effects 0.000 description 1
• 150000004679 hydroxides Chemical class 0.000 description 1
• 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
• 229960002591 hydroxyproline Drugs 0.000 description 1
• 230000001900 immune effect Effects 0.000 description 1
• 229940127121 immunoconjugate Drugs 0.000 description 1
• 230000005847 immunogenicity Effects 0.000 description 1
• 229940072221 immunoglobulins Drugs 0.000 description 1
• 238000002513 implantation Methods 0.000 description 1
• 230000002779 inactivation Effects 0.000 description 1
• 208000027866 inflammatory disease Diseases 0.000 description 1
• 230000004054 inflammatory process Effects 0.000 description 1
• 238000013383 initial experiment Methods 0.000 description 1
• 239000002198 insoluble material Substances 0.000 description 1
• 230000008611 intercellular interaction Effects 0.000 description 1
• 230000002452 interceptive effect Effects 0.000 description 1
• 229940047122 interleukins Drugs 0.000 description 1
• 230000035987 intoxication Effects 0.000 description 1
• 231100000566 intoxication Toxicity 0.000 description 1
• 230000008863 intramolecular interaction Effects 0.000 description 1
• 238000005342 ion exchange Methods 0.000 description 1
• 238000002955 isolation Methods 0.000 description 1
• AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
• 229960000310 isoleucine Drugs 0.000 description 1
• JJWLVOIRVHMVIS-UHFFFAOYSA-N isopropylamine Chemical compound CC(C)N JJWLVOIRVHMVIS-UHFFFAOYSA-N 0.000 description 1
• 210000001503 joint Anatomy 0.000 description 1
• 210000000231 kidney cortex Anatomy 0.000 description 1
• 230000029795 kidney development Effects 0.000 description 1
• 230000002147 killing effect Effects 0.000 description 1
• 239000004310 lactic acid Substances 0.000 description 1
• 235000014655 lactic acid Nutrition 0.000 description 1
• 239000010410 layer Substances 0.000 description 1
• 210000001232 limbus corneae Anatomy 0.000 description 1
• GZQKNULLWNGMCW-PWQABINMSA-N lipid A (E. coli) Chemical group O1[C@H](CO)[C@@H](OP(O)(O)=O)[C@H](OC(=O)C[C@@H](CCCCCCCCCCC)OC(=O)CCCCCCCCCCCCC)[C@@H](NC(=O)C[C@@H](CCCCCCCCCCC)OC(=O)CCCCCCCCCCC)[C@@H]1OC[C@@H]1[C@@H](O)[C@H](OC(=O)C[C@H](O)CCCCCCCCCCC)[C@@H](NC(=O)C[C@H](O)CCCCCCCCCCC)[C@@H](OP(O)(O)=O)O1 GZQKNULLWNGMCW-PWQABINMSA-N 0.000 description 1
• 239000006193 liquid solution Substances 0.000 description 1
• 239000006194 liquid suspension Substances 0.000 description 1
• 210000004185 liver Anatomy 0.000 description 1
• 244000144972 livestock Species 0.000 description 1
• 230000007774 longterm Effects 0.000 description 1
• 231100000053 low toxicity Toxicity 0.000 description 1
• 210000004072 lung Anatomy 0.000 description 1
• 210000002751 lymph Anatomy 0.000 description 1
• 125000003588 lysine group Chemical group [H]N([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 1
• 230000002101 lytic effect Effects 0.000 description 1
• 208000002780 macular degeneration Diseases 0.000 description 1
• 230000036244 malformation Effects 0.000 description 1
• 210000001161 mammalian embryo Anatomy 0.000 description 1
• 210000003593 megakaryocyte Anatomy 0.000 description 1
• 229910052751 metal Inorganic materials 0.000 description 1
• 239000002184 metal Substances 0.000 description 1
• 230000009401 metastasis Effects 0.000 description 1
• 208000037819 metastatic cancer Diseases 0.000 description 1
• 208000011575 metastatic malignant neoplasm Diseases 0.000 description 1
• 229930182817 methionine Natural products 0.000 description 1
• WBYWAXJHAXSJNI-UHFFFAOYSA-N methyl p-hydroxycinnamate Natural products OC(=O)C=CC1=CC=CC=C1 WBYWAXJHAXSJNI-UHFFFAOYSA-N 0.000 description 1
• HPNSFSBZBAHARI-UHFFFAOYSA-N micophenolic acid Natural products OC1=C(CC=C(C)CCC(O)=O)C(OC)=C(C)C2=C1C(=O)OC2 HPNSFSBZBAHARI-UHFFFAOYSA-N 0.000 description 1
• 238000001531 micro-dissection Methods 0.000 description 1
• 230000004089 microcirculation Effects 0.000 description 1
• 210000004088 microvessel Anatomy 0.000 description 1
• 230000001617 migratory effect Effects 0.000 description 1
• CFCUWKMKBJTWLW-BKHRDMLASA-N mithramycin Chemical compound O([C@@H]1C[C@@H](O[C@H](C)[C@H]1O)OC=1C=C2C=C3C[C@H]([C@@H](C(=O)C3=C(O)C2=C(O)C=1C)O[C@@H]1O[C@H](C)[C@@H](O)[C@H](O[C@@H]2O[C@H](C)[C@H](O)[C@H](O[C@@H]3O[C@H](C)[C@@H](O)[C@@](C)(O)C3)C2)C1)[C@H](OC)C(=O)[C@@H](O)[C@@H](C)O)[C@H]1C[C@@H](O)[C@H](O)[C@@H](C)O1 CFCUWKMKBJTWLW-BKHRDMLASA-N 0.000 description 1
• 230000000116 mitigating effect Effects 0.000 description 1
• 229960004857 mitomycin Drugs 0.000 description 1
• 230000011278 mitosis Effects 0.000 description 1
• 230000000394 mitotic effect Effects 0.000 description 1
• 108091005601 modified peptides Proteins 0.000 description 1
• 238000000302 molecular modelling Methods 0.000 description 1
• 230000008450 motivation Effects 0.000 description 1
• 229960000951 mycophenolic acid Drugs 0.000 description 1
• HPNSFSBZBAHARI-RUDMXATFSA-N mycophenolic acid Chemical compound OC1=C(C\C=C(/C)CCC(O)=O)C(OC)=C(C)C2=C1C(=O)OC2 HPNSFSBZBAHARI-RUDMXATFSA-N 0.000 description 1
• PSZYNBSKGUBXEH-UHFFFAOYSA-N naphthalene-1-sulfonic acid Chemical compound C1=CC=C2C(S(=O)(=O)O)=CC=CC2=C1 PSZYNBSKGUBXEH-UHFFFAOYSA-N 0.000 description 1
• 230000008693 nausea Effects 0.000 description 1
• 230000001338 necrotic effect Effects 0.000 description 1
• QZGIWPZCWHMVQL-UIYAJPBUSA-N neocarzinostatin chromophore Chemical compound O1[C@H](C)[C@H](O)[C@H](O)[C@@H](NC)[C@H]1O[C@@H]1C/2=C/C#C[C@H]3O[C@@]3([C@@H]3OC(=O)OC3)C#CC\2=C[C@H]1OC(=O)C1=C(O)C=CC2=C(C)C=C(OC)C=C12 QZGIWPZCWHMVQL-UIYAJPBUSA-N 0.000 description 1
• 210000002569 neuron Anatomy 0.000 description 1
• 229910017604 nitric acid Inorganic materials 0.000 description 1
• 229910052757 nitrogen Inorganic materials 0.000 description 1
• VIKNJXKGJWUCNN-XGXHKTLJSA-N norethisterone Chemical compound O=C1CC[C@@H]2[C@H]3CC[C@](C)([C@](CC4)(O)C#C)[C@@H]4[C@@H]3CCC2=C1 VIKNJXKGJWUCNN-XGXHKTLJSA-N 0.000 description 1
• 210000004940 nucleus Anatomy 0.000 description 1
• 210000004287 null lymphocyte Anatomy 0.000 description 1
• 239000003921 oil Substances 0.000 description 1
• 238000006384 oligomerization reaction Methods 0.000 description 1
• 230000005868 ontogenesis Effects 0.000 description 1
• 230000003287 optical effect Effects 0.000 description 1
• 150000007524 organic acids Chemical class 0.000 description 1
• 235000005985 organic acids Nutrition 0.000 description 1
• 229960003104 ornithine Drugs 0.000 description 1
• 210000001672 ovary Anatomy 0.000 description 1
• 235000006408 oxalic acid Nutrition 0.000 description 1
• 239000006179 pH buffering agent Substances 0.000 description 1
• 238000004091 panning Methods 0.000 description 1
• 229920002866 paraformaldehyde Polymers 0.000 description 1
• 230000005298 paramagnetic effect Effects 0.000 description 1
• 238000007911 parenteral administration Methods 0.000 description 1
• 230000007170 pathology Effects 0.000 description 1
• 239000000816 peptidomimetic Substances 0.000 description 1
• 210000003668 pericyte Anatomy 0.000 description 1
• 230000002572 peristaltic effect Effects 0.000 description 1
• 102000013415 peroxidase activity proteins Human genes 0.000 description 1
• 108040007629 peroxidase activity proteins Proteins 0.000 description 1
• 230000002085 persistent effect Effects 0.000 description 1
• 239000008177 pharmaceutical agent Substances 0.000 description 1
• 239000008024 pharmaceutical diluent Substances 0.000 description 1
• 229940124531 pharmaceutical excipient Drugs 0.000 description 1
• 239000008196 pharmacological composition Substances 0.000 description 1
• 239000012071 phase Substances 0.000 description 1
• 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 1
• QGVLYPPODPLXMB-QXYKVGAMSA-N phorbol Natural products C[C@@H]1[C@@H](O)[C@]2(O)[C@H]([C@H]3C=C(CO)C[C@@]4(O)[C@H](C=C(C)C4=O)[C@@]13O)C2(C)C QGVLYPPODPLXMB-QXYKVGAMSA-N 0.000 description 1
• 235000011007 phosphoric acid Nutrition 0.000 description 1
• 150000003016 phosphoric acids Chemical class 0.000 description 1
• 238000006303 photolysis reaction Methods 0.000 description 1
• 230000015843 photosynthesis, light reaction Effects 0.000 description 1
• 230000004962 physiological condition Effects 0.000 description 1
• 230000001766 physiological effect Effects 0.000 description 1
• 239000002504 physiological saline solution Substances 0.000 description 1
• 210000002826 placenta Anatomy 0.000 description 1
• 230000003169 placental effect Effects 0.000 description 1
• 229960003171 plicamycin Drugs 0.000 description 1
• 230000008488 polyadenylation Effects 0.000 description 1
• 230000001323 posttranslational effect Effects 0.000 description 1
• 229910052700 potassium Inorganic materials 0.000 description 1
• 239000011591 potassium Substances 0.000 description 1
• 235000011164 potassium chloride Nutrition 0.000 description 1
• 238000011533 pre-incubation Methods 0.000 description 1
• 239000002244 precipitate Substances 0.000 description 1
• 230000013823 prenylation Effects 0.000 description 1
• 230000002265 prevention Effects 0.000 description 1
• 230000001023 pro-angiogenic effect Effects 0.000 description 1
• MFDFERRIHVXMIY-UHFFFAOYSA-N procaine Chemical compound CCN(CC)CCOC(=O)C1=CC=C(N)C=C1 MFDFERRIHVXMIY-UHFFFAOYSA-N 0.000 description 1
• 229960004919 procaine Drugs 0.000 description 1
• 210000001236 prokaryotic cell Anatomy 0.000 description 1
• 230000009696 proliferative response Effects 0.000 description 1
• 230000001737 promoting effect Effects 0.000 description 1
• 238000011321 prophylaxis Methods 0.000 description 1
• 235000019260 propionic acid Nutrition 0.000 description 1
• 235000019833 protease Nutrition 0.000 description 1
• 230000009145 protein modification Effects 0.000 description 1
• 230000001185 psoriatic effect Effects 0.000 description 1
• 208000005333 pulmonary edema Diseases 0.000 description 1
• 229940107700 pyruvic acid Drugs 0.000 description 1
• 238000011002 quantification Methods 0.000 description 1
• 239000001397 quillaja saponaria molina bark Substances 0.000 description 1
• IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 1
• 239000011541 reaction mixture Substances 0.000 description 1
• 229940075993 receptor modulator Drugs 0.000 description 1
• 238000003259 recombinant expression Methods 0.000 description 1
• 238000011084 recovery Methods 0.000 description 1
• 230000023276 regulation of development, heterochronic Effects 0.000 description 1
• 230000008844 regulatory mechanism Effects 0.000 description 1
• 210000002254 renal artery Anatomy 0.000 description 1
• 230000003252 repetitive effect Effects 0.000 description 1
• 230000010076 replication Effects 0.000 description 1
• 206010039073 rheumatoid arthritis Diseases 0.000 description 1
• 229930182490 saponin Natural products 0.000 description 1
• 150000007949 saponins Chemical class 0.000 description 1
• 238000013391 scatchard analysis Methods 0.000 description 1
• 238000003345 scintillation counting Methods 0.000 description 1
• 150000003335 secondary amines Chemical group 0.000 description 1
• 239000006152 selective media Substances 0.000 description 1
• 230000035945 sensitivity Effects 0.000 description 1
• 238000000926 separation method Methods 0.000 description 1
• 108091006024 signal transducing proteins Proteins 0.000 description 1
• 102000034285 signal transducing proteins Human genes 0.000 description 1
• 230000019491 signal transduction Effects 0.000 description 1
• 238000002741 site-directed mutagenesis Methods 0.000 description 1
• 201000000849 skin cancer Diseases 0.000 description 1
• 210000000329 smooth muscle myocyte Anatomy 0.000 description 1
• 239000001632 sodium acetate Substances 0.000 description 1
• 235000017281 sodium acetate Nutrition 0.000 description 1
• 229910000029 sodium carbonate Inorganic materials 0.000 description 1
• 239000001488 sodium phosphate Substances 0.000 description 1
• 229910000162 sodium phosphate Inorganic materials 0.000 description 1
• 239000002904 solvent Substances 0.000 description 1
• 210000004988 splenocyte Anatomy 0.000 description 1
• 239000003381 stabilizer Substances 0.000 description 1
• 230000010473 stable expression Effects 0.000 description 1
• 238000010561 standard procedure Methods 0.000 description 1
• 150000003431 steroids Chemical class 0.000 description 1
• 239000000021 stimulant Substances 0.000 description 1
• 230000004936 stimulating effect Effects 0.000 description 1
• 210000001768 subcellular fraction Anatomy 0.000 description 1
• 229950000244 sulfanilic acid Drugs 0.000 description 1
• 239000006228 supernatant Substances 0.000 description 1
• 239000013589 supplement Substances 0.000 description 1
• 230000001629 suppression Effects 0.000 description 1
• 239000000725 suspension Substances 0.000 description 1
• 230000002194 synthesizing effect Effects 0.000 description 1
• 238000007910 systemic administration Methods 0.000 description 1
• 230000009885 systemic effect Effects 0.000 description 1
• 230000002123 temporal effect Effects 0.000 description 1
• CNHYKKNIIGEXAY-UHFFFAOYSA-N thiolan-2-imine Chemical compound N=C1CCCS1 CNHYKKNIIGEXAY-UHFFFAOYSA-N 0.000 description 1
• 150000003573 thiols Chemical group 0.000 description 1
• 229940104230 thymidine Drugs 0.000 description 1
• 230000036962 time dependent Effects 0.000 description 1
• 230000024033 toxin binding Effects 0.000 description 1
• VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 1
• 230000002103 transcriptional effect Effects 0.000 description 1
• 238000003151 transfection method Methods 0.000 description 1
• 238000012546 transfer Methods 0.000 description 1
• 230000009261 transgenic effect Effects 0.000 description 1
• 230000001052 transient effect Effects 0.000 description 1
• 238000003146 transient transfection Methods 0.000 description 1
• 108091005703 transmembrane proteins Proteins 0.000 description 1
• 230000000472 traumatic effect Effects 0.000 description 1
• 125000005270 trialkylamine group Chemical group 0.000 description 1
• PXSOHRWMIRDKMP-UHFFFAOYSA-N triaziquone Chemical compound O=C1C(N2CC2)=C(N2CC2)C(=O)C=C1N1CC1 PXSOHRWMIRDKMP-UHFFFAOYSA-N 0.000 description 1
• RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
• PZYFJWVGRGEWGO-UHFFFAOYSA-N trisodium;hydrogen peroxide;trioxido(oxo)vanadium Chemical compound [Na+].[Na+].[Na+].OO.OO.OO.[O-][V]([O-])([O-])=O PZYFJWVGRGEWGO-UHFFFAOYSA-N 0.000 description 1
• 230000005747 tumor angiogenesis Effects 0.000 description 1
• 125000001493 tyrosinyl group Chemical group [H]OC1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 1
• 210000003606 umbilical vein Anatomy 0.000 description 1
• 241000701447 unidentified baculovirus Species 0.000 description 1
• 241001515965 unidentified phage Species 0.000 description 1
• 239000004474 valine Substances 0.000 description 1
• 210000003556 vascular endothelial cell Anatomy 0.000 description 1
• 210000004509 vascular smooth muscle cell Anatomy 0.000 description 1
• 230000004862 vasculogenesis Effects 0.000 description 1
• 235000015112 vegetable and seed oil Nutrition 0.000 description 1
• 239000008158 vegetable oil Substances 0.000 description 1
• 239000003981 vehicle Substances 0.000 description 1
• 229960003048 vinblastine Drugs 0.000 description 1
• JXLYSJRDGCGARV-XQKSVPLYSA-N vincaleukoblastine Chemical compound C([C@@H](C[C@]1(C(=O)OC)C=2C(=CC3=C([C@]45[C@H]([C@@]([C@H](OC(C)=O)[C@]6(CC)C=CCN([C@H]56)CC4)(O)C(=O)OC)N3C)C=2)OC)C[C@@](C2)(O)CC)N2CCC2=C1NC1=CC=CC=C21 JXLYSJRDGCGARV-XQKSVPLYSA-N 0.000 description 1
• 239000002821 viper venom Substances 0.000 description 1
• 230000009278 visceral effect Effects 0.000 description 1
• XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
• 238000009736 wetting Methods 0.000 description 1
• 239000000080 wetting agent Substances 0.000 description 1
• 210000001325 yolk sac Anatomy 0.000 description 1
• 229950009268 zinostatin Drugs 0.000 description 1