CA2399809A1 - Domaine de proteines combinatoire - Google Patents

Domaine de proteines combinatoire Download PDF

Info

Publication number
CA2399809A1
CA2399809A1 CA002399809A CA2399809A CA2399809A1 CA 2399809 A1 CA2399809 A1 CA 2399809A1 CA 002399809 A CA002399809 A CA 002399809A CA 2399809 A CA2399809 A CA 2399809A CA 2399809 A1 CA2399809 A1 CA 2399809A1
Authority
CA
Canada
Prior art keywords
protein
chimaeric
amino acid
sequence
proteins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002399809A
Other languages
English (en)
Inventor
Lutz Riechmann
Greg Winter
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Domantis Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0002492A external-priority patent/GB0002492D0/en
Priority claimed from GB0016346A external-priority patent/GB0016346D0/en
Priority claimed from GB0019362A external-priority patent/GB0019362D0/en
Application filed by Domantis Ltd filed Critical Domantis Ltd
Publication of CA2399809A1 publication Critical patent/CA2399809A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

L'invention concerne un domaine de protéines pliées chimériques qui comprend au moins deux segments de séquence provenant de séquences d'acides aminés parentes non homologues. Chacun des segments de séquence n'est pas conçu ou choisi pour ne comporter qu'un seul élément structurel complet de protéine ni n'est conçu ou choisi pour ne comporter qu'un domaine de protéines entier et, en situation d'isolement, ne montre aucun pliage important à température de fusion de la protéine chimérique. L'invention concerne également des procédés de sélection de ces domaines et un domaine de protéines pliées chimériques qui comprend au moins deux segments de séquence qui partagent des séquences communes ou des séquences de régions communes dans le pli de protéine de leurs séquences parentes d'acides aminés, ladite région commune du pli séquence n'étant pas conçue ou choisie pour ne comporter qu'un seul élément structurel complet de protéine et chacun des segments de séquence n'étant pas conçu ou choisi pour ne comporter qu'un domaine de protéines entier. En situation d'isolement, par ailleurs, les segments de séquence ne montrent aucun pliage important à température de fusion de la protéine chimérique. L'invention concerne enfin des procédés de sélection de ces domaines.
CA002399809A 2000-02-03 2001-02-02 Domaine de proteines combinatoire Abandoned CA2399809A1 (fr)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
GB0002492A GB0002492D0 (en) 2000-02-03 2000-02-03 Combinatorial protein domains
GB0002492.7 2000-02-03
US18032600P 2000-02-04 2000-02-04
US60/180,326 2000-02-04
GB0016346.9 2000-07-03
GB0016346A GB0016346D0 (en) 2000-07-03 2000-07-03 Combinatorial protein domains
GB0019362A GB0019362D0 (en) 2000-08-07 2000-08-07 Combinational protein Domains
GB0019362.3 2000-08-07
PCT/GB2001/000445 WO2001057065A2 (fr) 2000-02-03 2001-02-02 Domaine de protéines combinatoire

Publications (1)

Publication Number Publication Date
CA2399809A1 true CA2399809A1 (fr) 2001-08-09

Family

ID=27447768

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002399809A Abandoned CA2399809A1 (fr) 2000-02-03 2001-02-02 Domaine de proteines combinatoire

Country Status (7)

Country Link
EP (1) EP1252319A2 (fr)
JP (1) JP2003523742A (fr)
AU (1) AU3040101A (fr)
CA (1) CA2399809A1 (fr)
GB (1) GB2375112A (fr)
NO (1) NO20023606L (fr)
WO (1) WO2001057065A2 (fr)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004101790A1 (fr) 2003-05-14 2004-11-25 Domantis Limited Procede de recuperation de polypeptides qui se deplient de façon reversible a partir d'un repertoire de polypeptides
KR20100018040A (ko) 2007-06-06 2010-02-16 도만티스 리미티드 프로테아제 내성 폴리펩티드를 선택하는 방법
CL2008003561A1 (es) 2007-11-30 2010-02-05 Glaxo Group Ltd Construccion de union a il - 13 que comprende a lo menos un dominio simple (dab) de anticuerpo injertado en un anticuerpo monoclonal ( mabdab); polinucleotido y celula huesped; procedimiento para preparar la construccion; composicion farmaceutica que la comprende y uso para tratar cancer o enfermedades inflamatorias.
WO2010063818A2 (fr) 2008-12-05 2010-06-10 Glaxo Group Limited Procédés de sélection de polypeptides résistants aux protéases
WO2010097394A1 (fr) 2009-02-24 2010-09-02 Glaxo Group Limited Constructions multivalentes et/ou multispécifiques de liaison au rankl
US20110305693A1 (en) 2009-02-24 2011-12-15 Glaxo Group Limited Anitigen-binding constructs
CA2753287A1 (fr) 2009-02-24 2010-09-02 Glaxo Group Limited Constructions de liaison d'antigene
US20130236467A1 (en) 2010-11-24 2013-09-12 Jeremy Griggs Multispecific antigen binding proteins targeting hgf
EP2736925A2 (fr) 2011-07-27 2014-06-04 Glaxo Group Limited Constructions de liaison à un antigène
EP3561703B1 (fr) * 2018-04-25 2021-01-20 Bayer AG Identification d'appariement de domaines variables des chaînes légères et lourdes d'anticorps
WO2023140360A1 (fr) * 2022-01-20 2023-07-27 京都府公立大学法人 Molécule mimétique d'anticorps

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2715940B1 (fr) * 1994-02-10 1996-04-26 Pasteur Merieux Serums Vacc Procédé de préparation d'une banque multicombinatoire de vecteurs d'expression de gènes d'anticorps, banque et systèmes d'expression d'anticorps "coliclonaux" obtenus.
WO1997032017A1 (fr) * 1996-02-26 1997-09-04 Morphosys Gesellschaft Für Proteinoptimierung Mbh Nouvelle methode pour l'identification de sequences d'acides nucleiques codant pour deux (poly)peptides interactifs, ou plus
EP1078051B1 (fr) * 1998-05-13 2007-12-12 Domantis Limited Systeme d'expression phagique pour la selection des proteines correctement pliees
GB9929061D0 (en) * 1999-12-08 2000-02-02 Medical Res Council Methods of producing novel enzymes

Also Published As

Publication number Publication date
JP2003523742A (ja) 2003-08-12
GB0217404D0 (en) 2002-09-04
WO2001057065A2 (fr) 2001-08-09
AU3040101A (en) 2001-08-14
EP1252319A2 (fr) 2002-10-30
NO20023606D0 (no) 2002-07-30
GB2375112A (en) 2002-11-06
NO20023606L (no) 2002-10-02
WO2001057065A3 (fr) 2002-01-31

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Legal Events

Date Code Title Description
FZDE Discontinued