CA2384123A1 - Promoteur et terminateur de glyceraldehyde-3-phosphate deshydrogenase 2 de pichia methanolica - Google Patents

Promoteur et terminateur de glyceraldehyde-3-phosphate deshydrogenase 2 de pichia methanolica Download PDF

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Publication number
CA2384123A1
CA2384123A1 CA002384123A CA2384123A CA2384123A1 CA 2384123 A1 CA2384123 A1 CA 2384123A1 CA 002384123 A CA002384123 A CA 002384123A CA 2384123 A CA2384123 A CA 2384123A CA 2384123 A1 CA2384123 A1 CA 2384123A1
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CA
Canada
Prior art keywords
dna
ala
gly
seq
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002384123A
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English (en)
Inventor
Christopher K. Raymond
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Zymogenetics Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2384123A1 publication Critical patent/CA2384123A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/01013—Glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) (1.2.1.13)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention concerne des séquences de promoteur de transcription et de terminateur utilisées dans le gène de glycéraldéhyde-3-phosphate déshydrogénase 2 (gène GAP2) issues de Pichia methanolica. Ces séquences sont utiles dans des constructions d'ADN permettant de produire des protéines considérées dans des cellules cultivées de P. methanolica. Dans les vecteurs d'expression, un promoteur et/ou un terminateur de GAP2 est lié, de manière fonctionnelle, à un segment d'ADN codant pour la protéine considérée.
CA002384123A 1999-09-08 2000-09-01 Promoteur et terminateur de glyceraldehyde-3-phosphate deshydrogenase 2 de pichia methanolica Abandoned CA2384123A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US39195199A 1999-09-08 1999-09-08
US09/391,951 1999-09-08
PCT/US2000/024110 WO2001018182A1 (fr) 1999-09-08 2000-09-01 Promoteur et terminateur de glyceraldehyde-3-phosphate deshydrogenase 2 de pichia methanolica

Publications (1)

Publication Number Publication Date
CA2384123A1 true CA2384123A1 (fr) 2001-03-15

Family

ID=23548649

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002384123A Abandoned CA2384123A1 (fr) 1999-09-08 2000-09-01 Promoteur et terminateur de glyceraldehyde-3-phosphate deshydrogenase 2 de pichia methanolica

Country Status (5)

Country Link
EP (1) EP1210412A1 (fr)
JP (1) JP2003509024A (fr)
AU (1) AU7103800A (fr)
CA (1) CA2384123A1 (fr)
WO (1) WO2001018182A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2401357B1 (fr) * 2009-02-26 2015-03-25 GlaxoSmithKline LLC Cellules hôtes et procédés d'utilisation

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000500014A (ja) * 1995-11-09 2000-01-11 ザイモジェネティクス，インコーポレイティド ピヒア・メタノリカ中で異種ポリペプチドを生産するための組成物と方法
DK1015577T3 (da) * 1997-09-15 2006-04-03 Zymogenetics Inc Kromosomal mutagenese i Pichia methanolica
WO2000056903A2 (fr) * 1999-03-22 2000-09-28 Zymogenetics, Inc. Methodes ameliorees de production de proteines dans la pichia transformee

Also Published As

Publication number Publication date
EP1210412A1 (fr) 2002-06-05
AU7103800A (en) 2001-04-10
WO2001018182A1 (fr) 2001-03-15
JP2003509024A (ja) 2003-03-11

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued