CA2382483A1 - Techniques et compositions permettant de construire et d'utiliser des bibliotheques de fusion - Google Patents

Techniques et compositions permettant de construire et d'utiliser des bibliotheques de fusion Download PDF

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Publication number
CA2382483A1
CA2382483A1 CA002382483A CA2382483A CA2382483A1 CA 2382483 A1 CA2382483 A1 CA 2382483A1 CA 002382483 A CA002382483 A CA 002382483A CA 2382483 A CA2382483 A CA 2382483A CA 2382483 A1 CA2382483 A1 CA 2382483A1
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CA
Canada
Prior art keywords
nucleic acid
protein
enzyme
nam
fusion
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002382483A
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English (en)
Inventor
Min Li
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School of Medicine of Johns Hopkins University
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Individual
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Application filed by Individual filed Critical Individual
Publication of CA2382483A1 publication Critical patent/CA2382483A1/fr
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'invention concerne des bibliothèques d'acides nucléiques de fusion, chacune comprenant des acides nucléiques codant pour un enzyme de modification d'acide nucléique (NAM), et un acide nucléique codant pour une protéine candidate. L'invention concerne également une bibliothèque de polypeptides de fusion comprenant un enzyme de modification d'acide nucléique (NAM), et une protéine candidate, notamment une bibliothèque de vecteurs d'expression, chaque vecteur comprenant i) un acide nucléique de fusion renfermant un acide nucléique codant pour un enzyme de modification d'acide nucléique (NAM), et un acide nucléique codant pour une protéine candidate, et ii) une séquence de fixation d'enzyme (EAS). Il existe au moins deux protéines candidates différentes. L'enzyme NAM est, de préférence, une protéine Rep, et EAS est, de préférence, plus long que 20 nucléotides. De même, des modes de réalisation préférés utilisent des acides nucléiques de fusion comprenant des acides nucléiques codant pour des structures de présentation, des acides nucléiques codant pour des marqueurs, ou des acides nucléiques codant pour des séquences cibles. L'invention concerne également des bibliothèques de conjugués d'acide nucléique/protéine (NAP), chacun renfermant un polypeptide de fusion comprenant un enzyme NAM et une protéine candidate. Les conjugués NAP comprennent également un vecteur d'expression comprenant un acide nucléique de fusion renfermant un acide nucléique codant pour un enzyme NAM, un acide nucléique codant pour une protéine candidate, et une séquence de fixation d'enzyme (EAS) reconnue par l'enzyme NAM. Les enzymes EAS et NAM sont fixés de manière covalente. L'invention concerne enfin des bibliothèques de cellules hôtes et des procédés de criblage.
CA002382483A 1999-08-20 2000-08-18 Techniques et compositions permettant de construire et d'utiliser des bibliotheques de fusion Abandoned CA2382483A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US15000499P 1999-08-20 1999-08-20
US60/150,004 1999-08-20
US20913000P 2000-06-02 2000-06-02
US60/209,130 2000-06-02
PCT/US2000/022906 WO2001014539A2 (fr) 1999-08-20 2000-08-18 Techniques et compositions permettant de construire et d'utiliser des bibliotheques de fusion

Publications (1)

Publication Number Publication Date
CA2382483A1 true CA2382483A1 (fr) 2001-03-01

Family

ID=26847232

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002382483A Abandoned CA2382483A1 (fr) 1999-08-20 2000-08-18 Techniques et compositions permettant de construire et d'utiliser des bibliotheques de fusion

Country Status (7)

Country Link
EP (1) EP1212411A2 (fr)
JP (1) JP2003507063A (fr)
KR (1) KR20020059370A (fr)
CN (1) CN1378593A (fr)
AU (1) AU781478B2 (fr)
CA (1) CA2382483A1 (fr)
WO (1) WO2001014539A2 (fr)

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US20020168640A1 (en) * 2001-02-22 2002-11-14 Min Li Biochips comprising nucleic acid/protein conjugates
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WO2002068453A2 (fr) * 2001-02-22 2002-09-06 Xencor Procedes et compositions pour la realisation et l'utilisation de librairies de fusion, au moyen de techniques d'elaboration informatique de proteines
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AU2003239899A1 (en) * 2002-05-24 2003-12-12 Somagenics, Inc. Methods and compositions for production of directed sequence libraries
US20090010920A1 (en) 2003-03-03 2009-01-08 Xencor, Inc. Fc Variants Having Decreased Affinity for FcyRIIb
US8883147B2 (en) 2004-10-21 2014-11-11 Xencor, Inc. Immunoglobulins insertions, deletions, and substitutions
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US9714282B2 (en) 2003-09-26 2017-07-25 Xencor, Inc. Optimized Fc variants and methods for their generation
WO2005053811A2 (fr) 2003-11-21 2005-06-16 Johns Hopkins University Motifs de separation biomoleculaire et utilisations
WO2005086679A2 (fr) 2004-03-05 2005-09-22 Medical College Of Ohio Procedes et compositions pour l'evaluation d'acides nucleiques et d'alleles
CA2568238C (fr) 2004-04-12 2013-10-15 Medical College Of Ohio Methodes et compositions pour analyser des analytes
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HUE044466T2 (hu) 2007-12-26 2019-10-28 Xencor Inc Megváltozott FcRn-kötõdési képességû Fc variánsok
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SG11201703281RA (en) 2014-11-14 2017-05-30 Voyager Therapeutics Inc Compositions and methods of treating amyotrophic lateral sclerosis (als)
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KR102175826B1 (ko) * 2015-05-11 2020-11-06 일루미나, 인코포레이티드 치료제의 발견 및 분석을 위한 플랫폼
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Also Published As

Publication number Publication date
WO2001014539A3 (fr) 2001-09-27
EP1212411A2 (fr) 2002-06-12
JP2003507063A (ja) 2003-02-25
KR20020059370A (ko) 2002-07-12
AU781478B2 (en) 2005-05-26
AU6792500A (en) 2001-03-19
WO2001014539A2 (fr) 2001-03-01
CN1378593A (zh) 2002-11-06

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EEER Examination request
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