CA2378657A1 - Identification et surexpression d'une sequence d'adn codant pour une 2-methyl-6-phytylhydroquinone-methyltransferase dans des plantes - Google Patents

Identification et surexpression d'une sequence d'adn codant pour une 2-methyl-6-phytylhydroquinone-methyltransferase dans des plantes Download PDF

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Publication number
CA2378657A1
CA2378657A1 CA002378657A CA2378657A CA2378657A1 CA 2378657 A1 CA2378657 A1 CA 2378657A1 CA 002378657 A CA002378657 A CA 002378657A CA 2378657 A CA2378657 A CA 2378657A CA 2378657 A1 CA2378657 A1 CA 2378657A1
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CA
Canada
Prior art keywords
leu
ala
gly
dna sequence
plants
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002378657A
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English (en)
Inventor
Karin Herbers
Ralf Badur
Irene Kunze
Michael Geiger
Hans-Peter Mock
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SunGene GmbH
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2378657A1 publication Critical patent/CA2378657A1/fr
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
    • C07H3/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

L'invention concerne un procédé pour produire des plantes présentant une teneur accrue en tocophérols et en tocotriénols, par surexpression d'un gène codant pour une 2-méthyl-6-phytylhydroquinone-méthyltransférase.
CA002378657A 1999-07-09 2000-06-23 Identification et surexpression d'une sequence d'adn codant pour une 2-methyl-6-phytylhydroquinone-methyltransferase dans des plantes Abandoned CA2378657A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE1999131834 DE19931834A1 (de) 1999-07-09 1999-07-09 Identifizierung und Überexpression einer DNA-Sequenz codierend für eine 2-Methyl-6-phytylhydrochinon-methyltransferase in Pflanzen
DE19931834.4 1999-07-09
PCT/EP2000/005862 WO2001004330A1 (fr) 1999-07-09 2000-06-23 Identification et surexpression d'une sequence d'adn codant pour une 2-methyl-6-phytylhydroquinone-methyltransferase dans des plantes

Publications (1)

Publication Number Publication Date
CA2378657A1 true CA2378657A1 (fr) 2001-01-18

Family

ID=7914084

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002378657A Abandoned CA2378657A1 (fr) 1999-07-09 2000-06-23 Identification et surexpression d'une sequence d'adn codant pour une 2-methyl-6-phytylhydroquinone-methyltransferase dans des plantes

Country Status (5)

Country Link
EP (1) EP1194577A1 (fr)
AU (1) AU5685800A (fr)
CA (1) CA2378657A1 (fr)
DE (1) DE19931834A1 (fr)
WO (1) WO2001004330A1 (fr)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR0010597A (pt) 1999-04-12 2002-02-13 Monsanto Technology Llc Plantas transgênicas contendo nìveis alterados de compostos esterol e tocoferóis
EP1171610B1 (fr) 1999-04-15 2007-04-04 Calgene LLC Sequences d'acide nucleique et proteines intervenant dans la synthese de l'isoprenoide
WO2000068393A1 (fr) 1999-05-07 2000-11-16 Pioneer Hi-Bred International, Inc. Acides nucleiques de transferase phytyle/prenyle, polypeptides, et leur utilisation
US6872815B1 (en) 2000-10-14 2005-03-29 Calgene Llc Nucleic acid sequences to proteins involved in tocopherol synthesis
AR030124A1 (es) 2000-08-07 2003-08-13 Monsanto Technology Llc Genes de la via del fosfato de metil-d-eritritol
EP1392106B1 (fr) 2001-05-09 2008-12-31 Monsanto Technology LLC Genes tyra et utilisations associees
US7244877B2 (en) 2001-08-17 2007-07-17 Monsanto Technology Llc Methyltransferase from cotton and uses thereof
US7262339B2 (en) 2001-10-25 2007-08-28 Monsanto Technology Llc Tocopherol methyltransferase tMT2 and uses thereof
WO2003080647A2 (fr) 2002-03-19 2003-10-02 Monsanto Technology, Llc Acides nucleiques et polypeptides se rapportant a l'enzyme homogentisate prenyl transferase ('hpt'), et utilisations correspondantes
DE10212703A1 (de) * 2002-03-21 2003-10-02 Sungene Gmbh & Co Kgaa Erhöhung Vitamin-E-Gehalts in Organismen durch Erhöhung der 2-Methyl-6-phytythydrochinon-Methyltransferase-Aktivität
WO2004013312A2 (fr) 2002-08-05 2004-02-12 Monsanto Technology, Llc Genes associes a la biosynthese du tocopherol et utilisation de ceux-ci
JP2007516704A (ja) * 2003-12-02 2007-06-28 ビーエーエスエフ　アクチェンゲゼルシャフト 除草剤の標的としての２−メチル−６−ソラニルベンゾキノン・メチルトランスフェラーゼ

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6642434B1 (en) * 1997-07-25 2003-11-04 University Of Community College System Of Nevada Transgenic plants with γ-tocopherol methyltransferase
US6448475B1 (en) * 1998-08-25 2002-09-10 The University And Community College System Of Nevada Manipulation of tocopherol levels in transgenic plants by transformation with a synechocystis 2-methyl-6-phytylplastoquinol/2-methyl-6-solanylplastoquinol-9 methyltransferase
AU2037700A (en) * 1998-12-03 2000-06-19 E.I. Du Pont De Nemours And Company Plant vitamin e biosynthetic enzymes

Also Published As

Publication number Publication date
EP1194577A1 (fr) 2002-04-10
AU5685800A (en) 2001-01-30
DE19931834A1 (de) 2001-01-11
WO2001004330A1 (fr) 2001-01-18

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FZDE Discontinued