CA2371176C - Assays for nucleoside diphosphates and triphosphates - Google Patents

Assays for nucleoside diphosphates and triphosphates Download PDF

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Publication number
CA2371176C
CA2371176C CA002371176A CA2371176A CA2371176C CA 2371176 C CA2371176 C CA 2371176C CA 002371176 A CA002371176 A CA 002371176A CA 2371176 A CA2371176 A CA 2371176A CA 2371176 C CA2371176 C CA 2371176C
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CA
Canada
Prior art keywords
ndpk
phosphoenzyme
label
nucleoside
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA002371176A
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English (en)
French (fr)
Other versions
CA2371176A1 (en
Inventor
Martin Hermann Klemens Brune
John Edgar Thomas Corrie
Martin Ronald Webb
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Francis Crick Institute Ltd
Original Assignee
Medical Research Council
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medical Research Council filed Critical Medical Research Council
Publication of CA2371176A1 publication Critical patent/CA2371176A1/en
Application granted granted Critical
Publication of CA2371176C publication Critical patent/CA2371176C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA002371176A 1999-05-10 2000-05-05 Assays for nucleoside diphosphates and triphosphates Expired - Lifetime CA2371176C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB9910811.0 1999-05-10
GBGB9910811.0A GB9910811D0 (en) 1999-05-10 1999-05-10 Assays
PCT/GB2000/001740 WO2000068418A1 (en) 1999-05-10 2000-05-05 Assays for nucleoside diphosphates and triphospates

Publications (2)

Publication Number Publication Date
CA2371176A1 CA2371176A1 (en) 2000-11-16
CA2371176C true CA2371176C (en) 2008-12-23

Family

ID=10853167

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002371176A Expired - Lifetime CA2371176C (en) 1999-05-10 2000-05-05 Assays for nucleoside diphosphates and triphosphates

Country Status (9)

Country Link
US (2) US6746849B1 (https=)
EP (1) EP1177314B1 (https=)
JP (1) JP4338320B2 (https=)
AT (1) ATE264400T1 (https=)
AU (1) AU771858B2 (https=)
CA (1) CA2371176C (https=)
DE (1) DE60009891T2 (https=)
GB (1) GB9910811D0 (https=)
WO (1) WO2000068418A1 (https=)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4431334B2 (ja) 2003-07-29 2010-03-10 独立行政法人科学技術振興機構 改良されたatpの増幅方法およびその利用
GB0711328D0 (en) * 2007-06-12 2007-07-25 Medical Res Council Biosensor for detection and visualisation of single-stranded dna
GB0817166D0 (en) * 2008-09-19 2008-10-29 Medical Res Council Sensor
CN103031287B (zh) * 2012-12-10 2014-12-10 浙江工业大学 冬虫夏草中国被毛孢二磷酸核苷激酶、编码基因及其应用
GB201505266D0 (en) * 2015-03-27 2015-05-13 Medical Res Council ATP sensor
CN106290166A (zh) * 2016-09-20 2017-01-04 江南大学 一种细胞内atp的圆二色光谱实时检测方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2834704A1 (de) * 1978-08-08 1980-02-21 Boehringer Mannheim Gmbh Verfahren zur quantitativen enzymatischen bestimmung von adp
IT1172385B (it) * 1983-12-21 1987-06-18 Miles Italiana Composizione e metodo per la determinazione enzimatica di atp ed fmn
FR2660933A1 (fr) 1990-04-12 1991-10-18 Pasteur Institut Moyens pour la detection de cellules hautement proliferatives.
US5741635A (en) 1996-01-30 1998-04-21 Mount Sinai Hospital Corporation Method of quantitating GTP and GDP bound to a G protein and uses thereof
WO2000052467A1 (en) * 1999-03-02 2000-09-08 The University Of Dundee Methods of determining altered ndpk functions

Also Published As

Publication number Publication date
AU771858B2 (en) 2004-04-01
DE60009891T2 (de) 2005-04-21
EP1177314B1 (en) 2004-04-14
EP1177314A1 (en) 2002-02-06
GB9910811D0 (en) 1999-07-07
US7297506B2 (en) 2007-11-20
JP2002543798A (ja) 2002-12-24
WO2000068418A1 (en) 2000-11-16
JP4338320B2 (ja) 2009-10-07
US20040248079A1 (en) 2004-12-09
AU4592300A (en) 2000-11-21
ATE264400T1 (de) 2004-04-15
DE60009891D1 (en) 2004-05-19
CA2371176A1 (en) 2000-11-16
US6746849B1 (en) 2004-06-08

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