CA2370478A1 - N-acylphosphoramidites and their use in oligonucleotide synthesis - Google Patents

N-acylphosphoramidites and their use in oligonucleotide synthesis Download PDF

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Publication number
CA2370478A1
CA2370478A1 CA002370478A CA2370478A CA2370478A1 CA 2370478 A1 CA2370478 A1 CA 2370478A1 CA 002370478 A CA002370478 A CA 002370478A CA 2370478 A CA2370478 A CA 2370478A CA 2370478 A1 CA2370478 A1 CA 2370478A1
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CA
Canada
Prior art keywords
alkyl
different
group
same
halogen
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
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CA002370478A
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English (en)
French (fr)
Inventor
Serge L. Beaucage
Andrzej Wilk
Andrzej Grajkowski
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Individual
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US Department of Health and Human Services
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by US Department of Health and Human Services filed Critical US Department of Health and Human Services
Publication of CA2370478A1 publication Critical patent/CA2370478A1/en
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups
CA002370478A 1999-03-24 2000-02-16 N-acylphosphoramidites and their use in oligonucleotide synthesis Abandoned CA2370478A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US12586799P 1999-03-24 1999-03-24
US60/125,867 1999-03-24
PCT/US2000/004032 WO2000056749A1 (en) 1999-03-24 2000-02-16 N-acylphosphoramidites and their use in oligonucleotide synthesis

Publications (1)

Publication Number Publication Date
CA2370478A1 true CA2370478A1 (en) 2000-09-28

Family

ID=22421815

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002370478A Abandoned CA2370478A1 (en) 1999-03-24 2000-02-16 N-acylphosphoramidites and their use in oligonucleotide synthesis

Country Status (5)

Country Link
US (2) US6965041B1 (US06762298-20040713-C00026.png)
EP (1) EP1163251A1 (US06762298-20040713-C00026.png)
AU (1) AU2998800A (US06762298-20040713-C00026.png)
CA (1) CA2370478A1 (US06762298-20040713-C00026.png)
WO (1) WO2000056749A1 (US06762298-20040713-C00026.png)

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US7355037B2 (en) 2001-12-03 2008-04-08 The United States Of America As Represented By The Department Of Health And Human Services Thermolabile hydroxyl protecting groups and methods of use
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WO2004101582A2 (en) 2003-05-09 2004-11-25 Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services Thermolabile hydroxyl protecting groups and methods of use
JP4865544B2 (ja) * 2004-03-25 2012-02-01 株式会社キラルジェン 立体規則性の高いリボヌクレオチド類縁体及びデオキシリボヌクレオチド類縁体の製造法
WO2006065751A2 (en) 2004-12-13 2006-06-22 Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services Cpg oligonucleotide prodrugs, compositions thereof and associated therapeutic methods
JP5558811B2 (ja) * 2006-06-01 2014-07-23 トリリンク バイオテクノロジーズ 核酸増幅のための化学的に修飾されたオリゴヌクレオチドプライマー
US7902345B2 (en) 2006-12-05 2011-03-08 Sequenom, Inc. Detection and quantification of biomolecules using mass spectrometry
WO2009032781A2 (en) 2007-08-29 2009-03-12 Sequenom, Inc. Methods and compositions for universal size-specific polymerase chain reaction
EP2205619A4 (en) * 2007-10-15 2013-04-17 Honeywell Int Inc SYNTHESIS OF OLIGONUCLEOTIDES OR A PHOSPHOROTHIOATE OLIGONUCLEOTIDE WITH A 1,3,5-TRIMETHYLHEXAHYDRO-1,3,5-TRIAZINE-FREE N-METHYLIMIDAZOLE STRIPPING AGENT
US8039612B2 (en) * 2007-10-15 2011-10-18 Honeywell International, Inc. Synthesis of oligonucleotides or phosphorothioate oligonucleotide with a capping agent of N-methylimidazole free of 1,3,5-trimethylhexahydro-1,3,5-triazine
CA2725239C (en) 2008-05-27 2019-02-26 Trilink Biotechnologies Chemically modified nucleoside 5'-triphosphates for thermally initiated amplification of nucleic acid
EP2408936A4 (en) * 2009-03-18 2013-01-30 Sequenom Inc USE OF HEAT-STAINED ENDONUCLEASES FOR THE PREPARATION OF REPORTER MOLECULES
US9631227B2 (en) 2009-07-06 2017-04-25 Trilink Biotechnologies, Inc. Chemically modified ligase cofactors, donors and acceptors
ES2521740T3 (es) * 2009-07-06 2014-11-13 Trilink Biotechnologies Cofactores, dadores y aceptores de ligasa modificados químicamente
US9556124B2 (en) 2013-10-31 2017-01-31 Honeywell International Inc. Methods for removing triazine from N-methylimidazole for synthesis of oligonucleotide
CN113584020A (zh) 2015-09-21 2021-11-02 垂林克生物技术公司 用于合成5’-加帽rna的组合物和方法
US20230271998A1 (en) 2020-06-30 2023-08-31 United States Of America, As Represented By The Secretary, Department Of Health And Human Services Solid support for synthesizing nucleic acid sequences and methods for making and using
WO2023147352A1 (en) 2022-01-27 2023-08-03 Trilink Biotechnologies, Llc Trinucleotide cap analogs and methods of use thereof

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Also Published As

Publication number Publication date
US6762298B2 (en) 2004-07-13
US20010044529A1 (en) 2001-11-22
AU2998800A (en) 2000-10-09
US6965041B1 (en) 2005-11-15
EP1163251A1 (en) 2001-12-19
WO2000056749A1 (en) 2000-09-28

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