CA2368903A1 - Detection de changements dans le nombre de copies d'un chromosome, permettant de distinguer les naevi melanocytiques des melanomes malins - Google Patents

Detection de changements dans le nombre de copies d'un chromosome, permettant de distinguer les naevi melanocytiques des melanomes malins Download PDF

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Publication number
CA2368903A1
CA2368903A1 CA002368903A CA2368903A CA2368903A1 CA 2368903 A1 CA2368903 A1 CA 2368903A1 CA 002368903 A CA002368903 A CA 002368903A CA 2368903 A CA2368903 A CA 2368903A CA 2368903 A1 CA2368903 A1 CA 2368903A1
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Canada
Prior art keywords
probe
sample
nucleic acid
hybridization
detecting
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
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CA002368903A
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English (en)
Inventor
Boris Bastian
Daniel Pinkel
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University of California
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Individual
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Publication date
Priority claimed from US09/288,940 external-priority patent/US6261775B1/en
Priority claimed from US09/541,364 external-priority patent/US6551780B1/en
Application filed by Individual filed Critical Individual
Publication of CA2368903A1 publication Critical patent/CA2368903A1/fr
Abandoned legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57407—Specifically defined cancers
    • G01N33/5743—Specifically defined cancers of skin, e.g. melanoma
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/112—Disease subtyping, staging or classification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Oncology (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Hospice & Palliative Care (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

La présente invention concerne des méthodes permettant de distinguer les naevi mélanocytiques, par exemple les naevi de Spitz, des mélanomes malins. Ces méthodes consistent à mettre en contact un échantillon d'acide nucléique d'un patient avec une sonde se fixant de manière sélective sur une séquence polynucléotidique cible sur une région chromosomique, par exemple 11p, généralement amplifiée dans les naevi de Spitz. L'échantillon d'acide nucléique provient en général de cellules tumorales cutanées d'une lésion tumorale cutanée du patient. Au moyen d'une autre sonde se fixant de manière sélective sur une région chromosomique, par exemple 1q, 6p, 7p, 9p, ou 10q, présentant en général un changement dans le nombre de copies du mélanome, la méthode de l'invention permet de déterminer que les cellules tumorales ne présentant aucun changement dans le nombre de copies de 1q, 6p, 7p, 9p, ou 10q ne sont pas des cellules de mélanome mais bien des cellules de naevus de Spitz. La découverte d'amplifications du chromosome 11p et, en particulier, la présence d'un isochromosome 11p, constituerait une indication supplémentaire de la présence du naevus de Spitz. On peut également déterminer une augmentation du nombre de copies du chromosome 11p grâce à la détection de la présence d'une amplification du gène H-RAS. Le gène amplifié peut être normal ou peut être un gène H-RAS mutant.
CA002368903A 1999-04-09 2000-04-10 Detection de changements dans le nombre de copies d'un chromosome, permettant de distinguer les naevi melanocytiques des melanomes malins Abandoned CA2368903A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US09/288,940 US6261775B1 (en) 1999-04-09 1999-04-09 Detection of chromosome copy number changes to distinguish melanocytic nevi from malignant melanoma
US09/288,940 2000-03-31
US09/541,364 US6551780B1 (en) 2000-03-31 2000-03-31 Detection of gene mutations or amplifications in melanocytic neoplasms
US09/541,364 2000-03-31
PCT/US2000/009609 WO2000061814A1 (fr) 1999-04-09 2000-04-10 Detection de changements dans le nombre de copies d'un chromosome, permettant de distinguer les naevi melanocytiques des melanomes malins

Publications (1)

Publication Number Publication Date
CA2368903A1 true CA2368903A1 (fr) 2000-10-19

Family

ID=26965345

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002368903A Abandoned CA2368903A1 (fr) 1999-04-09 2000-04-10 Detection de changements dans le nombre de copies d'un chromosome, permettant de distinguer les naevi melanocytiques des melanomes malins

Country Status (4)

Country Link
EP (1) EP1181392A4 (fr)
JP (1) JP2002541826A (fr)
CA (1) CA2368903A1 (fr)
WO (1) WO2000061814A1 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU647741B2 (en) * 1989-12-01 1994-03-31 Regents Of The University Of California, The Methods and compositions for chromosome-specific staining
WO2003027638A2 (fr) * 2001-09-27 2003-04-03 Spectral Genomics, Inc. Procedes pour detecter des mosaicismes genetiques au moyen de reseaux
WO2005052308A1 (fr) 2003-11-25 2005-06-09 Baker Hughes Incorporated Packer gonflable a couche gonflante
WO2007028031A2 (fr) * 2005-09-02 2007-03-08 The Regents Of The University Of California Methodes et combinaisons de sondes pour la detection de melanomes
US7960110B2 (en) 2006-11-15 2011-06-14 The Regents Of The University Of California Detection of chromosomal region copy number changes to diagnose melanoma
CN113817777B (zh) * 2021-10-27 2023-07-14 上海交通大学医学院附属第九人民医院 来源于人的先天性巨大黑痣良性肿瘤细胞系及其构建方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6465180B1 (en) * 1999-03-17 2002-10-15 The Regents Of The University Of California Detection of premalignant melanocytes

Also Published As

Publication number Publication date
WO2000061814A8 (fr) 2001-05-25
WO2000061814A1 (fr) 2000-10-19
WO2000061814A9 (fr) 2001-06-28
EP1181392A4 (fr) 2005-03-16
JP2002541826A (ja) 2002-12-10
EP1181392A1 (fr) 2002-02-27

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued