CA2366069A1 - Generation de mutants de levure conditionnels, techniques et reactifs s'y rapportant - Google Patents

Generation de mutants de levure conditionnels, techniques et reactifs s'y rapportant Download PDF

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Publication number
CA2366069A1
CA2366069A1 CA002366069A CA2366069A CA2366069A1 CA 2366069 A1 CA2366069 A1 CA 2366069A1 CA 002366069 A CA002366069 A CA 002366069A CA 2366069 A CA2366069 A CA 2366069A CA 2366069 A1 CA2366069 A1 CA 2366069A1
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CA
Canada
Prior art keywords
condition
sensitive
gene
yeast
restrictive
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002366069A
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English (en)
Inventor
Vivian Berlin
Csilla Csank
Susan E. Smith
Donald Sullivan
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Agennix USA Inc
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Individual
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Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2366069A1 publication Critical patent/CA2366069A1/fr
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/905—Stable introduction of foreign DNA into chromosome using homologous recombination in yeast

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne une technique permettant de générer de façon systématique des souches de mutant de levure d'haploïde et de diploïde obligatoires, dont les mutations létales d'un gène sont sensibles aux conditions, gène qui, par ailleurs, est essentiel à la viabilité cellulaire sous toutes les conditions de la croissance cellulaire. Les mutants conditionnels permettent l'analyse des changements physiologiques induits par l'inactivation d'un gène ou d'un produit génique et ils peuvent être utilisés pour solliciter la fonction d'un gène quelconque. Dans un mode de réalisation de l'invention, la technique consiste en général à: (i) prendre une souche de Candida hétérozygote en vue d'une mutation de perte de fonction d'un allèle de gène essentiel; (ii) et introduire un homologue conditionnellement sensible de ce gène, par exemple avec une protéine conditionnellement sensible ou une séquence de régulation de la transcription conditionnellement sensible, au niveau de l'allèle fonctionnel par transformation intégrative.
CA002366069A 1999-03-11 2000-03-13 Generation de mutants de levure conditionnels, techniques et reactifs s'y rapportant Abandoned CA2366069A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US12380799P 1999-03-11 1999-03-11
US60/123,807 1999-03-11
PCT/US2000/006514 WO2000053781A1 (fr) 1999-03-11 2000-03-13 Generation de mutants de levure conditionnels, techniques et reactifs s'y rapportant

Publications (1)

Publication Number Publication Date
CA2366069A1 true CA2366069A1 (fr) 2000-09-14

Family

ID=22411011

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002366069A Abandoned CA2366069A1 (fr) 1999-03-11 2000-03-13 Generation de mutants de levure conditionnels, techniques et reactifs s'y rapportant

Country Status (4)

Country Link
EP (1) EP1161544A1 (fr)
AU (1) AU3526200A (fr)
CA (1) CA2366069A1 (fr)
WO (1) WO2000053781A1 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6783985B1 (en) 2000-02-18 2004-08-31 Elitra Pharmaceuticals Inc. Gene disruption methodologies for drug target discovery
CA2398861A1 (fr) * 2000-02-18 2001-08-23 Elitra Pharmaceuticals, Inc. Methodologies de disruption genique destinees a la decouverte de medicaments cibles
US20030180953A1 (en) * 2000-12-29 2003-09-25 Elitra Pharmaceuticals, Inc. Gene disruption methodologies for drug target discovery
EP1932921A1 (fr) * 2006-12-13 2008-06-18 Novartis AG Procédé de criblage différentiel à base mutante simple pour des composés modulant une cible spécifique

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5756305A (en) * 1996-05-06 1998-05-26 Millennium Pharmaceuticals, Inc. Identification of essential survival genes

Also Published As

Publication number Publication date
WO2000053781A1 (fr) 2000-09-14
EP1161544A1 (fr) 2001-12-12
AU3526200A (en) 2000-09-28

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Legal Events

Date Code Title Description
FZDE Discontinued