CA2363297C - Mise au point de voies de sialylation intracellulaires - Google Patents

Mise au point de voies de sialylation intracellulaires Download PDF

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Publication number
CA2363297C
CA2363297C CA2363297A CA2363297A CA2363297C CA 2363297 C CA2363297 C CA 2363297C CA 2363297 A CA2363297 A CA 2363297A CA 2363297 A CA2363297 A CA 2363297A CA 2363297 C CA2363297 C CA 2363297C
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Prior art keywords
cell
cmp
cells
leu
lys
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Expired - Lifetime
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CA2363297A
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CA2363297A1 (fr
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Michael J. Betenbaugh
Shawn Lawrence
Yuan C. Lee
Timothy A. Coleman
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Human Genome Sciences Inc
Johns Hopkins University
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Human Genome Sciences Inc
Johns Hopkins University
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Publication of CA2363297A1 publication Critical patent/CA2363297A1/fr
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01052—Beta-N-acetylhexosaminidase (3.2.1.52)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1081—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/026—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a baculovirus

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention concerne des méthodes de manipulation des voies de traitement des glucides dans des cellules étudiées. Ces méthodes consistent à manipuler plusieurs voies impliquées dans la réaction de sialylation par utilisation des techniques de l'ADN de recombinaison et de l'alimentation en substrat, de manière à permettre la production des glycoprotéines sialylées dans les cellules étudiées. Parmi ces travaux de manipulation réalisés sur les glucides, figurent la mise en oeuvre de nouveaux dosages biologiques, l'examen d'une sélection de lignées cellulaires d'insectes et l'utilisation de la bioinformatique pour l'identification de séquences géniques destinées au traitement critique des enzymes. L'invention concerne également des compositions renfermant les cellules étudiées produisant des glycoprotéines sialylées. Ces méthodes et ces compositions sont utilisées pour l'expression hétérologue des glycoprotéines.
CA2363297A 1999-03-02 2000-03-01 Mise au point de voies de sialylation intracellulaires Expired - Lifetime CA2363297C (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US12258299P 1999-03-02 1999-03-02
US60/122,582 1999-03-02
US16962499P 1999-12-08 1999-12-08
US60/169,624 1999-12-08
PCT/US2000/005313 WO2000052135A2 (fr) 1999-03-02 2000-03-01 Mise au point de voies de sialylation intracellulaires

Publications (2)

Publication Number Publication Date
CA2363297A1 CA2363297A1 (fr) 2000-09-08
CA2363297C true CA2363297C (fr) 2011-08-09

Family

ID=26820692

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2363297A Expired - Lifetime CA2363297C (fr) 1999-03-02 2000-03-01 Mise au point de voies de sialylation intracellulaires

Country Status (5)

Country Link
EP (1) EP1399538A4 (fr)
JP (1) JP2003524395A (fr)
AU (1) AU3508300A (fr)
CA (1) CA2363297C (fr)
WO (1) WO2000052135A2 (fr)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6949372B2 (en) 1999-03-02 2005-09-27 The Johns Hopkins University Engineering intracellular sialylation pathways
AU2001236739A1 (en) * 2000-02-08 2001-08-20 Genentech Inc. Improved sialylation of glycoproteins
US7863020B2 (en) 2000-06-28 2011-01-04 Glycofi, Inc. Production of sialylated N-glycans in lower eukaryotes
DE60303591T2 (de) * 2002-03-07 2007-03-01 Eidgenössische Technische Hochschule Zürich System und methode zur herstellung von rekombinanten, glykosylierten protein in prokaryontischen wirtszellen
KR20130101592A (ko) 2002-03-07 2013-09-13 아이드게노쉬쉐 테흐니쉐 호흐슐레 쥬리히 원핵 숙주에서의 재조합 글리코실화 단백질 생산 방법 및 생산계
JP2006333701A (ja) * 2003-01-15 2006-12-14 Kazuhito Fujiyama 動物型糖鎖をもつ糖タンパク質の生産方法
AU2005215889B9 (en) * 2004-02-13 2011-11-17 Glycotope Gmbh Highly active glycoproteins-process conditions and an efficient method for their production
EP2365089B1 (fr) * 2004-03-17 2014-05-14 GlycoFi, Inc. Procédé d'ingénierie d'une voie de synthèse de l'acide cytidine monophosphate-sialique chez les levures
EP1758614A4 (fr) * 2004-05-04 2007-09-12 Univ Singapore Methode d'expression de glycoproteines sialylees dans des cellules mammiferes et cellules associees
US7479549B2 (en) 2005-02-23 2009-01-20 Jaques John Scott T Recombinant canine thyroid stimulating hormone and methods of production and use thereof
US8753864B2 (en) 2005-05-11 2014-06-17 Eth Zurich Recombinant N-glycosylated proteins from procaryotic cells
ES2348857T3 (es) * 2005-07-12 2010-12-03 Greenovation Biotech Gmbh Mejoras en o relacionadas con la produccion de proteinas.
AU2007294122B2 (en) 2006-09-10 2013-03-07 Glycotope Gmbh Use of human cells of myeloid leukaemia origin for expression of antibodies
EP1920782A1 (fr) 2006-11-10 2008-05-14 Glycotope Gmbh Microorganismes, ou fractions desdits microorganismes, capables d'induir de l' immunité cellulaire specifique contre des hydrates de carbone
LT2257307T (lt) 2008-02-20 2018-08-10 Glaxosmithkline Biologicals S.A. Biokonjugatai, gauti iš rekombinantinių n-glikozilintų baltymų iš prokariotinių ląstelių
KR101855380B1 (ko) 2009-11-19 2018-05-08 글락소스미스클라인 바이오로지칼즈 에스.에이. 원핵세포에서 면역원성 폴리사카라이드를 제조하는 생합성 시스템
CA2798381C (fr) 2010-05-06 2020-10-06 Glycovaxyn Ag Vaccins de bioconjugue de bacteries gram positif capsulaire
EP2412815A1 (fr) * 2010-07-27 2012-02-01 Universite De Rouen N-glycosylation dans le phaeodctylum tricornutum transformé
KR101898302B1 (ko) * 2010-10-15 2018-09-13 제이씨알 파마 가부시키가이샤 당사슬의 비환원 말단이 만노오스 잔기인 당 단백질의 제조 방법
CA2844543A1 (fr) 2011-08-22 2013-02-28 Glycotope Gmbh Microorganismes portant un antigene tumoral
SG11202010493XA (en) 2018-05-18 2020-11-27 Glycotope Gmbh Anti-muc1 antibody

Also Published As

Publication number Publication date
CA2363297A1 (fr) 2000-09-08
EP1399538A2 (fr) 2004-03-24
JP2003524395A (ja) 2003-08-19
AU3508300A (en) 2000-09-21
WO2000052135A2 (fr) 2000-09-08
EP1399538A4 (fr) 2004-03-24
WO2000052135A9 (fr) 2001-10-11
WO2000052135A3 (fr) 2004-01-08

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