CA2363297C - Mise au point de voies de sialylation intracellulaires - Google Patents

Mise au point de voies de sialylation intracellulaires Download PDF

Info

Publication number
CA2363297C
CA2363297C CA2363297A CA2363297A CA2363297C CA 2363297 C CA2363297 C CA 2363297C CA 2363297 A CA2363297 A CA 2363297A CA 2363297 A CA2363297 A CA 2363297A CA 2363297 C CA2363297 C CA 2363297C
Authority
CA
Canada
Prior art keywords
cell
cmp
cells
leu
lys
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA2363297A
Other languages
English (en)
Other versions
CA2363297A1 (fr
Inventor
Michael J. Betenbaugh
Shawn Lawrence
Yuan C. Lee
Timothy A. Coleman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Human Genome Sciences Inc
Johns Hopkins University
Original Assignee
Human Genome Sciences Inc
Johns Hopkins University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Human Genome Sciences Inc, Johns Hopkins University filed Critical Human Genome Sciences Inc
Publication of CA2363297A1 publication Critical patent/CA2363297A1/fr
Application granted granted Critical
Publication of CA2363297C publication Critical patent/CA2363297C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01052—Beta-N-acetylhexosaminidase (3.2.1.52)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1081—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/026—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a baculovirus

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention concerne des méthodes de manipulation des voies de traitement des glucides dans des cellules étudiées. Ces méthodes consistent à manipuler plusieurs voies impliquées dans la réaction de sialylation par utilisation des techniques de l'ADN de recombinaison et de l'alimentation en substrat, de manière à permettre la production des glycoprotéines sialylées dans les cellules étudiées. Parmi ces travaux de manipulation réalisés sur les glucides, figurent la mise en oeuvre de nouveaux dosages biologiques, l'examen d'une sélection de lignées cellulaires d'insectes et l'utilisation de la bioinformatique pour l'identification de séquences géniques destinées au traitement critique des enzymes. L'invention concerne également des compositions renfermant les cellules étudiées produisant des glycoprotéines sialylées. Ces méthodes et ces compositions sont utilisées pour l'expression hétérologue des glycoprotéines.
CA2363297A 1999-03-02 2000-03-01 Mise au point de voies de sialylation intracellulaires Expired - Lifetime CA2363297C (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US12258299P 1999-03-02 1999-03-02
US60/122,582 1999-03-02
US16962499P 1999-12-08 1999-12-08
US60/169,624 1999-12-08
PCT/US2000/005313 WO2000052135A2 (fr) 1999-03-02 2000-03-01 Mise au point de voies de sialylation intracellulaires

Publications (2)

Publication Number Publication Date
CA2363297A1 CA2363297A1 (fr) 2000-09-08
CA2363297C true CA2363297C (fr) 2011-08-09

Family

ID=26820692

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2363297A Expired - Lifetime CA2363297C (fr) 1999-03-02 2000-03-01 Mise au point de voies de sialylation intracellulaires

Country Status (5)

Country Link
EP (1) EP1399538A2 (fr)
JP (1) JP2003524395A (fr)
AU (1) AU3508300A (fr)
CA (1) CA2363297C (fr)
WO (1) WO2000052135A2 (fr)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6949372B2 (en) 1999-03-02 2005-09-27 The Johns Hopkins University Engineering intracellular sialylation pathways
AU2001236739A1 (en) * 2000-02-08 2001-08-20 Genentech Inc. Improved sialylation of glycoproteins
US20050260729A1 (en) * 2004-03-17 2005-11-24 Hamilton Stephen R Method of engineering a cytidine monophosphate-sialic acid synthetic pathway in fungi and yeast
US7863020B2 (en) 2000-06-28 2011-01-04 Glycofi, Inc. Production of sialylated N-glycans in lower eukaryotes
KR100938026B1 (ko) 2002-03-07 2010-01-21 아이드게노쉬쉐 테흐니쉐 호흐슐레 쥬리히 원핵 숙주에서 재조합 글리코실화 단백질의 생산방법 및생산계
PT1481057E (pt) 2002-03-07 2006-05-31 Eidgenoess Tech Hochschule Sistema e metodo para a producao de proteinas glicosiladas recombinantes num hospedeiro procariota
JP2006333701A (ja) * 2003-01-15 2006-12-14 Kazuhito Fujiyama 動物型糖鎖をもつ糖タンパク質の生産方法
CA2557725C (fr) * 2004-02-13 2015-06-30 Glycotope Gmbh Conditions de traitement de glycoproteines extremement actives et methode efficace de production associee
US20090298120A1 (en) * 2004-05-04 2009-12-03 National University Of Singapore Method for expressing sialylated glycoproteins in mammalian cells and cells thereof
US7479549B2 (en) 2005-02-23 2009-01-20 Jaques John Scott T Recombinant canine thyroid stimulating hormone and methods of production and use thereof
CN103396478B (zh) 2005-05-11 2016-09-28 Eth苏黎世公司 来自原核细胞的重组n-糖基化蛋白
DK1904636T3 (da) 2005-07-12 2010-10-18 Greenovation Biotech Gmbh Forbedringer inden for eller i sammenhæng med proteinfremstilling
EP2428224B1 (fr) 2006-09-10 2016-12-21 Glycotope GmbH Utilisation des cellules humaines provenant d'une leucémie myéloïde pour l'expression d'anticorps
PL1920781T3 (pl) 2006-11-10 2015-06-30 Glycotope Gmbh Kompozycje zawierające core-1-dodatnie mikroorganizmy i ich zastosowanie w leczeniu lub profilaktyce nowotworów
LT2257307T (lt) 2008-02-20 2018-08-10 Glaxosmithkline Biologicals S.A. Biokonjugatai, gauti iš rekombinantinių n-glikozilintų baltymų iš prokariotinių ląstelių
JP5968784B2 (ja) 2009-11-19 2016-08-10 グリコヴァキシン アーゲー 原核細胞における免疫原性多糖体生成の生合成システム
SG185433A1 (en) 2010-05-06 2012-12-28 Glycovaxyn Ag Capsular gram-positive bacteria bioconjugate vaccines
EP2412815A1 (fr) * 2010-07-27 2012-02-01 Universite De Rouen N-glycosylation dans le phaeodctylum tricornutum transformé
WO2012050175A1 (fr) * 2010-10-15 2012-04-19 日本ケミカルリサーチ株式会社 Procédé de production de glycoprotéines ayant un résidu mannose comme extrémité non réductrice de la chaîne sucrée
BR112014004065A2 (pt) 2011-08-22 2017-03-14 Glycotope Gmbh micro-organismos carregando um antígeno de tumor
DK3794042T3 (da) 2018-05-18 2024-04-15 Daiichi Sankyo Co Ltd Anti-muc1-exatecet-antistof-lægemiddelkonjugat

Also Published As

Publication number Publication date
WO2000052135A9 (fr) 2001-10-11
CA2363297A1 (fr) 2000-09-08
EP1399538A4 (fr) 2004-03-24
JP2003524395A (ja) 2003-08-19
AU3508300A (en) 2000-09-21
EP1399538A2 (fr) 2004-03-24
WO2000052135A3 (fr) 2004-01-08
WO2000052135A2 (fr) 2000-09-08

Similar Documents

Publication Publication Date Title
US6949372B2 (en) Engineering intracellular sialylation pathways
CA2363297C (fr) Mise au point de voies de sialylation intracellulaires
Harrison et al. Protein N‐glycosylation in the baculovirus–insect cell expression system and engineering of insect cells to produce “mammalianized” recombinant glycoproteins
Aumiller et al. A transgenic insect cell line engineered to produce CMP–sialic acid and sialylated glycoproteins
Shi et al. Protein N-glycosylation in the baculovirus-insect cell system
Lawrence et al. Cloning and expression of the humanN-acetylneuraminic acid phosphate synthase gene with 2-Keto-3-deoxy-d-glycero-d-galacto-nononic acid biosynthetic ability
US20170159095A1 (en) Method of production of recombinant glycoproteins with increased circulatory half-life in mammalian cells
Kim et al. Expression of a functional Drosophila melanogaster N-acetylneuraminic acid (Neu5Ac) phosphate synthase gene: evidence for endogenous sialic acid biosynthetic ability in insects
Geisler et al. Substrate specificities and intracellular distributions of three N-glycan processing enzymes functioning at a key branch point in the insect N-glycosylation pathway
CA2558635A1 (fr) Procede de mise au point d'une voie de synthese du monophosphate de cytidine-acide sialique dans les champignons et la levure
Clark et al. Gene‐expression profiles for five key glycosylation genes for galactose‐fed CHO cells expressing recombinant IL‐4/13 cytokine trap
US6323332B1 (en) Sulfotransferase for HNK-1 glycan
US9012176B2 (en) Chinese hamster ovary cell lines
US5856159A (en) Production of galactosyltransferase
Zheng et al. Molecular cloning and characterization of a novel α1, 2-fucosyltransferase (CE2FT-1) from Caenorhabditis elegans
JP2003047467A (ja) コンドロイチン合成酵素
WO2001042492A1 (fr) Procede de creation par genie genetique de trajets de sialylation intracellulaires
US20070154982A1 (en) Mammalian cell lines modified for the production of recombinant glycoproteins
Kokuho et al. Molecular cloning and expression profile analysis of a novel β‐D‐N‐acetylhexosaminidase of domestic silkworm (Bombyx mori)
Zheng et al. A novel α1, 2-fucosyltransferase (CE2FT-2) in Caenorhabditis elegans generates H-type 3 glycan structures
WO2001059075A1 (fr) Amelioration concernant la sialylation de glycoproteines
KR100446010B1 (ko) 신규 재조합 배큘로바이러스 발현벡터 및 이를 이용한효소의 제조방법
US20130029413A1 (en) Methods of Using a Bacterial GlcNAc-6-P 2'- Epimerase to Promote Sialylation of Glycoconjugates
KR20070060439A (ko) 곤충세포주에 시알산 전이효소 유전자 및 갈락토오스전이효소 유전자가 형질전환된 당사슬 부가기능이 향상된형질전환 곤충세포주 및 그 제조 방법
US6762038B1 (en) Mutant cell lines and methods for producing enhanced levels of recombinant proteins

Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry

Effective date: 20200302