CA2338185A1 - Complexe in vitro thermostable a activite de polymerase - Google Patents

Complexe in vitro thermostable a activite de polymerase Download PDF

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Publication number
CA2338185A1
CA2338185A1 CA002338185A CA2338185A CA2338185A1 CA 2338185 A1 CA2338185 A1 CA 2338185A1 CA 002338185 A CA002338185 A CA 002338185A CA 2338185 A CA2338185 A CA 2338185A CA 2338185 A1 CA2338185 A1 CA 2338185A1
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CA
Canada
Prior art keywords
glu
leu
lys
ile
val
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002338185A
Other languages
English (en)
Inventor
Christian Kilger
Ingo Kober
Hartmut Voss
Gerd Moeckel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sygnis Pharma AG
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE19840771A external-priority patent/DE19840771A1/de
Application filed by Individual filed Critical Individual
Publication of CA2338185A1 publication Critical patent/CA2338185A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Le complexe in vitro thermostable selon la présente invention destiné à l'élongation, dépendante de matrices, d'acide nucléique comporte une protéine agrafe thermostable et une protéine d'élongation thermostable.
CA002338185A 1998-08-06 1999-08-06 Complexe in vitro thermostable a activite de polymerase Abandoned CA2338185A1 (fr)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
DE19835653 1998-08-06
DE19835653.6 1998-08-06
DE19840771A DE19840771A1 (de) 1998-08-06 1998-09-07 Thermostabiler in vitro-Komplex mit Polymeraseaktivität
DE19840771.8 1998-09-07
EP99111795.3 1999-06-18
EP99111795 1999-06-18
PCT/DE1999/002480 WO2000008164A2 (fr) 1998-08-06 1999-08-06 Complexe in vitro thermostable a activite de polymerase

Publications (1)

Publication Number Publication Date
CA2338185A1 true CA2338185A1 (fr) 2000-02-17

Family

ID=56289931

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002338185A Abandoned CA2338185A1 (fr) 1998-08-06 1999-08-06 Complexe in vitro thermostable a activite de polymerase

Country Status (5)

Country Link
EP (1) EP1100923A2 (fr)
JP (1) JP2002522042A (fr)
AU (1) AU5617199A (fr)
CA (1) CA2338185A1 (fr)
WO (1) WO2000008164A2 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2792651B1 (fr) * 1999-04-21 2005-03-18 Centre Nat Rech Scient Sequence genomique et polypeptides de pyrococcus abyssi, leurs fragments et leurs utilisations
DE19937230A1 (de) * 1999-08-06 2001-02-08 Lion Bioscience Ag Chimäre Proteine
US6627424B1 (en) * 2000-05-26 2003-09-30 Mj Bioworks, Inc. Nucleic acid modifying enzymes
JP2005511012A (ja) * 2001-08-10 2005-04-28 セローノ ジェネティクス インスティテュート ソシエテ アノニム ヒトｃＤＮＡおよびタンパク質およびその使用
JP6699560B2 (ja) * 2014-11-28 2020-05-27 東洋紡株式会社 Ｐｃｎａ単量体
WO2016084879A1 (fr) * 2014-11-28 2016-06-02 東洋紡株式会社 Dispositif d'amplification d'acide nucléique
WO2017090685A1 (fr) * 2015-11-27 2017-06-01 国立大学法人九州大学 Mutant d'adn polymérase

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5583026A (en) * 1994-08-31 1996-12-10 Cornell Research Foundation, Inc. Process for reconstituting the polymerase III* and other subassemblies of E. coli DNA polymerase III holoenzyme from peptide subunits
WO1993015115A1 (fr) * 1992-01-24 1993-08-05 Cornell Research Foundation, Inc. HOLOENZYME ADN POLYMERASE III et E. COLI ET SOUS-UNITES
US5633159A (en) * 1995-03-10 1997-05-27 Becton, Dickinson And Company DNA polymerase III β-subunit from mycobacteriophage DS6A
AU7683998A (en) * 1997-04-08 1998-10-30 Rockfeller University, The Enzyme derived from thermophilic organisms that functions as a chromosomal replicase, and preparation and uses thereof
US6238905B1 (en) * 1997-09-12 2001-05-29 University Technology Corporation Thermophilic polymerase III holoenzyme
WO1999053074A1 (fr) * 1998-04-09 1999-10-21 The Rockfeller University Enzyme derivee d'organismes termophiles qui fonctionnent comme une replicase chromosomique, sa preparation et ses utilisations

Also Published As

Publication number Publication date
AU5617199A (en) 2000-02-28
JP2002522042A (ja) 2002-07-23
WO2000008164A3 (fr) 2000-05-11
EP1100923A2 (fr) 2001-05-23
WO2000008164A2 (fr) 2000-02-17

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