CA2335324A1 - Procedes de detection et de mappage de genes, de mutations et de sequences de polynucleotides du type variant - Google Patents

Procedes de detection et de mappage de genes, de mutations et de sequences de polynucleotides du type variant Download PDF

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Publication number
CA2335324A1
CA2335324A1 CA002335324A CA2335324A CA2335324A1 CA 2335324 A1 CA2335324 A1 CA 2335324A1 CA 002335324 A CA002335324 A CA 002335324A CA 2335324 A CA2335324 A CA 2335324A CA 2335324 A1 CA2335324 A1 CA 2335324A1
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CA
Canada
Prior art keywords
probe
linker
reporter
gene
tag
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002335324A
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English (en)
Inventor
David A. Shafer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genetag Technology Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2335324A1 publication Critical patent/CA2335324A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6862—Ligase chain reaction [LCR]

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention comprend un certain nombre de structures apparentées destinées à des constituants de sonde génique, à des constituants assurant des liaisons multiples et à des constituants de signalisation, qui sont tous modulaires par nature et qui peuvent être utilisés ensemble ou en partiellement. Ces constituants sont en général reliés ensemble dans des structures composites par hybridation de sous-segments complémentaires, appelés lieurs. Les reporteurs de la présente invention sont également prévus pour être accolés dans des réseaux qui peuvent assurer la signalisation amplifiée. Les constituants assurant des liaisons multiples de la présente invention peuvent être intercalés entre la sonde et les unités reporteur et assurer la liaison de plusieurs reporteurs. Ces sondes et ces procédés de signalisation peuvent également comprendre des systèmes qui permettent d'effectuer le marquage à couleurs mixtes qui est spécifique à chaque cible. La présente invention est utile pour détecter des séquences cibles présentes dans une grande diversité de formats comprenant sans limitation aucune, les formats de membrane, les formats in situ et divers formats de puce à substrat solide.
CA002335324A 1998-07-17 1999-07-16 Procedes de detection et de mappage de genes, de mutations et de sequences de polynucleotides du type variant Abandoned CA2335324A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US9321998P 1998-07-17 1998-07-17
US60/093,219 1998-07-17
PCT/US1999/016242 WO2000004192A1 (fr) 1998-07-17 1999-07-16 Procedes de detection et de mappage de genes, de mutations et de sequences de polynucleotides du type variant

Publications (1)

Publication Number Publication Date
CA2335324A1 true CA2335324A1 (fr) 2000-01-27

Family

ID=22237801

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002335324A Abandoned CA2335324A1 (fr) 1998-07-17 1999-07-16 Procedes de detection et de mappage de genes, de mutations et de sequences de polynucleotides du type variant

Country Status (4)

Country Link
EP (1) EP1112378A1 (fr)
AU (1) AU5003299A (fr)
CA (1) CA2335324A1 (fr)
WO (1) WO2000004192A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3480320A4 (fr) * 2016-06-30 2020-03-11 Biois Co., Ltd. Sonde de signal d'acide nucléique bicaténaire et procédé de détection de molécule cible utilisant celle-ci

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001066802A1 (fr) * 2000-03-09 2001-09-13 Genetag Technology, Inc. Systemes et procedes de quantification et amplification a la fois d'elements de signalisation et de sondes, dans des puces d'adnc et des matrices de micro-echantillons d'expression
US8673570B2 (en) * 2000-03-09 2014-03-18 Genetag Technology, Inc. System and methods to quantify and amplify both signaling and probes for cDNA chips and gene expression microarrays
US6893822B2 (en) * 2001-07-19 2005-05-17 Nanogen Recognomics Gmbh Enzymatic modification of a nucleic acid-synthetic binding unit conjugate
EP1595953B1 (fr) * 2003-02-21 2012-04-04 Eisai R&D Management Co., Ltd. Procede d'amplification de signal pour detecter un gene mutant
WO2005061733A1 (fr) * 2003-11-20 2005-07-07 Singulex, Inc. Preparation de sondes etiquetees hautement definies
GB0404187D0 (en) * 2004-02-25 2004-03-31 Biotransformations Ltd Binding agents
JP2005304489A (ja) * 2004-03-24 2005-11-04 Sysmex Corp 標的物質検出用プローブセット及び標的物質検出方法。
US8076067B2 (en) * 2006-08-15 2011-12-13 Genetag Technology, Inc. Probe-antiprobe compositions and methods for DNA or RNA detection
US8685899B2 (en) * 2007-02-14 2014-04-01 Genisphere Inc. Methods, reagents and kits for detection of nucleic acid molecules
CA2697032C (fr) 2007-08-22 2021-09-14 The Regents Of The University Of California Polypeptides de liaison activables et procedes d'identification et utilisation de ceux-ci
EP2385955B1 (fr) 2009-01-12 2020-08-12 CytomX Therapeutics, Inc. Compositions d anticorps modifiées et leurs procédés de production et d utilisation
CN102481341B (zh) 2009-02-23 2017-05-17 希托马克斯医疗有限公司 蛋白原及其使用方法
EP2949759B1 (fr) 2014-05-27 2017-07-26 Université Paris Sud 11 Détection multiplexe homogène d'oligonucléotides
WO2017127741A1 (fr) * 2016-01-22 2017-07-27 Grail, Inc. Procédés et systèmes de séquençage haute fidélité
CN112601823A (zh) * 2018-06-12 2021-04-02 安可济控股有限公司 用于形成连接产物的方法和组合物
CN112805394B (zh) * 2018-12-07 2024-03-19 深圳华大生命科学研究院 长片段核酸测序的方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5359100A (en) * 1987-10-15 1994-10-25 Chiron Corporation Bifunctional blocked phosphoramidites useful in making nucleic acid mutimers
WO1992017484A1 (fr) * 1991-03-27 1992-10-15 Research Corporation Technologies, Inc. Oligonucleotides circulaires a brin unique
JPH09500378A (ja) * 1993-07-02 1997-01-14 リンクス セラピューティクス，インコーポレイティド 枝分れされそして複雑に結合された高分子構造体の集中的合成
FR2710075B1 (fr) * 1993-09-15 1995-10-27 Bio Merieux Réactif et procédé pour la détection d'une séquence nucléotidique avec amplification de signal.
US5681697A (en) * 1993-12-08 1997-10-28 Chiron Corporation Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor
US5912124A (en) * 1996-06-14 1999-06-15 Sarnoff Corporation Padlock probe detection
US5853993A (en) * 1996-10-21 1998-12-29 Hewlett-Packard Company Signal enhancement method and kit

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3480320A4 (fr) * 2016-06-30 2020-03-11 Biois Co., Ltd. Sonde de signal d'acide nucléique bicaténaire et procédé de détection de molécule cible utilisant celle-ci

Also Published As

Publication number Publication date
EP1112378A1 (fr) 2001-07-04
AU5003299A (en) 2000-02-07
WO2000004192A1 (fr) 2000-01-27
WO2000004192A8 (fr) 2000-07-13

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