CA2334712A1 - Detection of ligands with signal amplification - Google Patents
Detection of ligands with signal amplification Download PDFInfo
- Publication number
- CA2334712A1 CA2334712A1 CA002334712A CA2334712A CA2334712A1 CA 2334712 A1 CA2334712 A1 CA 2334712A1 CA 002334712 A CA002334712 A CA 002334712A CA 2334712 A CA2334712 A CA 2334712A CA 2334712 A1 CA2334712 A1 CA 2334712A1
- Authority
- CA
- Canada
- Prior art keywords
- receptor
- ligand
- enzyme
- binding
- antibody
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
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- 230000003321 amplification Effects 0.000 title claims abstract description 42
- 238000003199 nucleic acid amplification method Methods 0.000 title claims abstract description 42
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
- G01N33/545—Synthetic resin
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Inorganic Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Silicon Polymers (AREA)
- Absorbent Articles And Supports Therefor (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/095,196 | 1998-06-10 | ||
| US09/095,196 US6171802B1 (en) | 1998-06-10 | 1998-06-10 | Detection and amplification of ligands |
| PCT/US1999/010413 WO1999064862A1 (en) | 1998-06-10 | 1999-05-12 | Detection of ligands with signal amplification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2334712A1 true CA2334712A1 (en) | 1999-12-16 |
Family
ID=22250600
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002334712A Abandoned CA2334712A1 (en) | 1998-06-10 | 1999-05-12 | Detection of ligands with signal amplification |
Country Status (10)
| Country | Link |
|---|---|
| US (5) | US6171802B1 (https=) |
| EP (1) | EP1086374B1 (https=) |
| JP (1) | JP4377058B2 (https=) |
| AT (1) | ATE287539T1 (https=) |
| AU (1) | AU753758B2 (https=) |
| BR (1) | BR9910982A (https=) |
| CA (1) | CA2334712A1 (https=) |
| DE (1) | DE69923313D1 (https=) |
| IL (2) | IL139911A0 (https=) |
| WO (1) | WO1999064862A1 (https=) |
Families Citing this family (40)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020052002A1 (en) * | 1998-06-10 | 2002-05-02 | Niehaus Gary D. | Detection and amplification of ligands |
| US6171802B1 (en) * | 1998-06-10 | 2001-01-09 | Kent State University | Detection and amplification of ligands |
| US6537631B1 (en) * | 1999-04-30 | 2003-03-25 | Kimberly-Clark Worldwide, Inc. | Roll of wet wipes |
| AU774638B2 (en) * | 2000-02-16 | 2004-07-01 | Wisconsin Alumni Research Foundation | Method and apparatus for detection of microscopic pathogens |
| KR100463979B1 (ko) * | 2000-02-16 | 2005-01-03 | 위스콘신 얼럼나이 리서어치 화운데이션 | 액정 분석을 위한 생화학적 블로킹층 |
| US7135143B2 (en) | 2001-03-14 | 2006-11-14 | Wisconsin Alumni Research Foundation | Detecting compounds with liquid crystals |
| US6411354B1 (en) | 2001-05-11 | 2002-06-25 | Kent State University | Bulk alignment of lyotropic chromonic liquid crystals |
| US6673398B2 (en) | 2001-05-14 | 2004-01-06 | Kent State University | Alignment of lyotropic chromonic liquid crystals at surfaces as monolayers and multilayered stacks |
| US7666661B2 (en) | 2001-08-27 | 2010-02-23 | Platypus Technologies, Llc | Substrates, devices, and methods for quantitative liquid crystal assays |
| ATE438883T1 (de) * | 2001-09-04 | 2009-08-15 | Wisconsin Alumni Res Found | Flüssigkristallumschaltmechanismus |
| WO2003029481A2 (en) * | 2001-10-04 | 2003-04-10 | Wisconsin Alumni Research Foundation | Detection of dna hybridization on surfaces |
| US7807348B2 (en) * | 2002-03-20 | 2010-10-05 | Wisconsin Alumni Research Foundation | Optical imaging of nanostructured substrates |
| US7125592B2 (en) * | 2002-04-10 | 2006-10-24 | Wisconsin Alumni Research Foundation | Detecting interactions at biomimetic interfaces with liquid crystals |
| EP2093565B1 (en) | 2002-05-22 | 2014-11-19 | Platypus Technologies, Inc. | Uses of devices and methods for cellular assays |
| US20030232340A1 (en) * | 2002-06-13 | 2003-12-18 | David Anderson | Nanoporous particle with a retained target |
| WO2004044583A1 (en) * | 2002-11-08 | 2004-05-27 | Wisconsin Alumni Research Foundation | Surfaces with gradients in surface topography |
| US7060225B2 (en) * | 2003-03-20 | 2006-06-13 | Northeastern Ohio Universities College Of Medicine | Self-contained assay device for rapid detection of biohazardous agents |
| WO2005010160A2 (en) * | 2003-07-17 | 2005-02-03 | Wisconsin Alumni Research Foundation | Liquid crystals with reduced toxicity and applications thereof |
| AU2004262301A1 (en) | 2003-07-25 | 2005-02-10 | Platypus Technologies, Llc | Liquid crystal based analyte detection |
| US20050069928A1 (en) * | 2003-08-04 | 2005-03-31 | Blue Heron Biotechnology, Inc. | Methods for synthesis of defined polynucleotides |
| US7795007B2 (en) | 2003-09-23 | 2010-09-14 | Wisconsin Alumni Research Foundation | Detection of post-translationally modified peptides with liquid crystals |
| JP4668911B2 (ja) * | 2003-09-23 | 2011-04-13 | ウィスコンシン アルムニ リサーチ ファンデイション | アフィニティー・マイクロコンタクトプリントされた生体分子を検出するための液晶の使用 |
| CA2545482A1 (en) | 2003-11-10 | 2005-05-26 | Platypus Technologies, Llc | Substrates, devices, and methods for cellular assays |
| US7531366B2 (en) * | 2004-07-23 | 2009-05-12 | Platypus Technologies, Llc | Bead based assays using a liquid crystal reporter |
| US7294370B2 (en) * | 2004-08-17 | 2007-11-13 | Kent State University | Aligned lyotropic chromonic liquid crystal films |
| WO2007025129A2 (en) * | 2005-08-25 | 2007-03-01 | Platypus Technologies, Llc. | Compositions and liquid crystals |
| US7407815B2 (en) * | 2005-09-28 | 2008-08-05 | Kent State University | Liquid crystal cassette |
| US8586389B2 (en) * | 2005-09-28 | 2013-11-19 | Kent State University | Ligand concentrating, liquid crystal biosensor system |
| US20070099306A1 (en) * | 2005-09-28 | 2007-05-03 | Kent State University | Liquid crystal sensor system |
| WO2007111681A2 (en) * | 2005-10-31 | 2007-10-04 | Wisconsin Alumni Research Foundation | Device and methods for liquid crystal-based bioagent detection |
| EP2057277B1 (en) | 2006-08-07 | 2018-06-13 | Platypus Technologies, LLC | Substrates, devices, and methods for cellular assays |
| US7842499B2 (en) * | 2006-08-07 | 2010-11-30 | Platypus Technologies, Llc | Substrates, devices, and methods for cellular assays |
| EP2158627A2 (en) * | 2007-05-04 | 2010-03-03 | Akermin, Inc. | Immobilized enzymes and uses thereof |
| US9968935B2 (en) * | 2007-08-20 | 2018-05-15 | Platypus Technologies, Llc | Devices for cell assays |
| US8472683B2 (en) * | 2008-05-09 | 2013-06-25 | General Electric Company | Motion correction in tomographic images |
| US7947492B2 (en) * | 2008-08-20 | 2011-05-24 | Northeastern Ohio Universities College Of Medicine | Device improving the detection of a ligand |
| WO2010031047A2 (en) * | 2008-09-15 | 2010-03-18 | Platypus Technologies, Llc | Detection of vapor phase compounds by changes in physical properties of a liquid crystal |
| US9080973B2 (en) | 2009-04-22 | 2015-07-14 | Wisconsin Alumni Research Foundation | Analyte detection using liquid crystals |
| US8404318B2 (en) * | 2009-06-16 | 2013-03-26 | Kent State University | Methods and apparatus to produce aligned film of lyotropic chromonic liquid crystals |
| WO2017087659A1 (en) | 2015-11-18 | 2017-05-26 | Platypus Technologies, Llc | Liquid crystal sensor |
Family Cites Families (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4067774A (en) * | 1971-05-14 | 1978-01-10 | Syva Company | Compounds for enzyme amplification assay |
| US4203802A (en) * | 1971-05-14 | 1980-05-20 | Syva Company | Inhibitable enzyme amplification assay |
| US3817837A (en) * | 1971-05-14 | 1974-06-18 | Syva Corp | Enzyme amplification assay |
| US3975237A (en) * | 1972-11-06 | 1976-08-17 | Syva Company | Compounds for enzyme amplification assay - - ecgonine analogs |
| US4604364A (en) | 1974-01-04 | 1986-08-05 | Kosak Kenneth M | Bioluminescent tracer composition and method of use in immunoassays |
| US4600690A (en) * | 1978-08-07 | 1986-07-15 | Albert Einstein College Of Medicine Of Yeshiva University, A Division Of Yeshiva University | Immunoassay |
| US5156965A (en) * | 1983-11-29 | 1992-10-20 | Igen, Inc. | Catalytic antibodies |
| US5096807A (en) * | 1985-03-06 | 1992-03-17 | Murex Corporation | Imaging immunoassay detection system with background compensation and its use |
| JPS61271460A (ja) | 1985-05-28 | 1986-12-01 | Olympus Optical Co Ltd | 免疫学的分析方法 |
| US4693970A (en) * | 1985-06-21 | 1987-09-15 | Becton, Dickinson And Company | Immunoassay using complement |
| US5149626A (en) | 1987-05-14 | 1992-09-22 | Mclean Hospital Corporation | Multiple antigen immunoassay |
| US5447837A (en) | 1987-08-05 | 1995-09-05 | Calypte, Inc. | Multi-immunoassay diagnostic system for antigens or antibodies or both |
| IL83451A (en) * | 1987-08-06 | 1991-06-10 | Univ Ramot | Stabilized water soluble enzymes and a method for their preparation |
| US4943525A (en) | 1987-11-02 | 1990-07-24 | Bioventures, Inc. | Simultaneous immunoassay for the determination of antigens and antibodies |
| US5252459A (en) | 1988-09-23 | 1993-10-12 | Abbott Laboratories | Indicator reagents, diagnostic assays and test kits employing organic polymer latex particles |
| CA2108451A1 (en) * | 1991-04-26 | 1992-10-27 | Beverley J. Randle | Novel antibodies, and methods for their use |
| US5596434A (en) * | 1993-09-24 | 1997-01-21 | University Research Corporation | Self-assembled monolayers for liquid crystal alignment |
| US5681571A (en) | 1993-10-08 | 1997-10-28 | Duotol Ab | Immunological tolerance-inducing agent |
| EP0764195A1 (en) | 1994-06-06 | 1997-03-26 | Hoechst Celanese Corporation | Thermotropic liquid crystalline poly(esteramides) |
| GB9417593D0 (en) * | 1994-09-01 | 1994-10-19 | Secr Defence | Luciferase labelling method |
| US6207369B1 (en) * | 1995-03-10 | 2001-03-27 | Meso Scale Technologies, Llc | Multi-array, multi-specific electrochemiluminescence testing |
| US6001571A (en) | 1995-11-30 | 1999-12-14 | Mandecki; Wlodek | Multiplex assay for nucleic acids employing transponders |
| GB9712818D0 (en) * | 1996-07-08 | 1997-08-20 | Cambridge Antibody Tech | Labelling and selection of specific binding molecules |
| GB9622524D0 (en) * | 1996-10-29 | 1997-01-08 | London Biotechnology Ltd | Enzyme labels for assays |
| US5763158A (en) * | 1997-02-06 | 1998-06-09 | The United States Of America As Represented By The Secretary Of The Army | Detection of multiple antigens or antibodies |
| PT942780E (pt) | 1997-09-09 | 2003-11-28 | Lyotropic Therapeutics Inc | Particulas revestidas processos de obtencao e uso |
| US6284197B1 (en) | 1998-06-05 | 2001-09-04 | The Regents Of The University Of California | Optical amplification of molecular interactions using liquid crystals |
| US20020052002A1 (en) * | 1998-06-10 | 2002-05-02 | Niehaus Gary D. | Detection and amplification of ligands |
| US6171802B1 (en) * | 1998-06-10 | 2001-01-09 | Kent State University | Detection and amplification of ligands |
| AU1763701A (en) | 1999-11-12 | 2001-06-06 | 3M Innovative Properties Company | Liquid crystal alignment structures and optical devices containing same |
-
1998
- 1998-06-10 US US09/095,196 patent/US6171802B1/en not_active Expired - Lifetime
-
1999
- 1999-05-12 AU AU39844/99A patent/AU753758B2/en not_active Ceased
- 1999-05-12 IL IL13991199A patent/IL139911A0/xx active IP Right Grant
- 1999-05-12 BR BR9910982-4A patent/BR9910982A/pt not_active IP Right Cessation
- 1999-05-12 WO PCT/US1999/010413 patent/WO1999064862A1/en not_active Ceased
- 1999-05-12 EP EP99922970A patent/EP1086374B1/en not_active Expired - Lifetime
- 1999-05-12 JP JP2000553806A patent/JP4377058B2/ja not_active Expired - Fee Related
- 1999-05-12 DE DE69923313T patent/DE69923313D1/de not_active Expired - Lifetime
- 1999-05-12 AT AT99922970T patent/ATE287539T1/de not_active IP Right Cessation
- 1999-05-12 CA CA002334712A patent/CA2334712A1/en not_active Abandoned
-
2000
- 2000-11-26 IL IL139911A patent/IL139911A/en not_active IP Right Cessation
-
2002
- 2002-04-26 US US10/133,610 patent/US7267957B2/en not_active Expired - Fee Related
-
2007
- 2007-05-21 US US11/751,108 patent/US20070243593A1/en not_active Abandoned
- 2007-05-21 US US11/751,065 patent/US7927827B2/en not_active Expired - Fee Related
- 2007-09-10 US US11/852,662 patent/US7732219B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| IL139911A (en) | 2008-06-05 |
| US7732219B2 (en) | 2010-06-08 |
| JP2002517756A (ja) | 2002-06-18 |
| EP1086374A1 (en) | 2001-03-28 |
| US7267957B2 (en) | 2007-09-11 |
| US6171802B1 (en) | 2001-01-09 |
| US20070243593A1 (en) | 2007-10-18 |
| US20070059780A1 (en) | 2007-03-15 |
| BR9910982A (pt) | 2001-02-13 |
| AU753758B2 (en) | 2002-10-31 |
| ATE287539T1 (de) | 2005-02-15 |
| IL139911A0 (en) | 2002-02-10 |
| US20070218508A1 (en) | 2007-09-20 |
| US7927827B2 (en) | 2011-04-19 |
| US20080138244A1 (en) | 2008-06-12 |
| JP4377058B2 (ja) | 2009-12-02 |
| EP1086374B1 (en) | 2005-01-19 |
| DE69923313D1 (de) | 2005-02-24 |
| WO1999064862A1 (en) | 1999-12-16 |
| AU3984499A (en) | 1999-12-30 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| FZDE | Discontinued |