CA2330252A1 - Method of generating nucleic acid hybrids for mutation analysis - Google Patents

Method of generating nucleic acid hybrids for mutation analysis Download PDF

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Publication number
CA2330252A1
CA2330252A1 CA002330252A CA2330252A CA2330252A1 CA 2330252 A1 CA2330252 A1 CA 2330252A1 CA 002330252 A CA002330252 A CA 002330252A CA 2330252 A CA2330252 A CA 2330252A CA 2330252 A1 CA2330252 A1 CA 2330252A1
Authority
CA
Canada
Prior art keywords
sequences
primers
sense strand
complementary
linker
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002330252A
Other languages
English (en)
French (fr)
Inventor
Andrew Wallace
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CENTRAL MANCHESTER HEALTHCARE NHS TRUST
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2330252A1 publication Critical patent/CA2330252A1/en
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA002330252A 1998-06-12 1999-06-14 Method of generating nucleic acid hybrids for mutation analysis Abandoned CA2330252A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB9812674.1 1998-06-12
GBGB9812674.1A GB9812674D0 (en) 1998-06-12 1998-06-12 Nucleic acids
PCT/GB1999/001691 WO1999064624A2 (en) 1998-06-12 1999-06-14 Method of generating nucleic acid hybrids for mutation analysis

Publications (1)

Publication Number Publication Date
CA2330252A1 true CA2330252A1 (en) 1999-12-16

Family

ID=10833627

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002330252A Abandoned CA2330252A1 (en) 1998-06-12 1999-06-14 Method of generating nucleic acid hybrids for mutation analysis

Country Status (6)

Country Link
EP (1) EP1086250A2 (de)
JP (1) JP2002517258A (de)
AU (1) AU5377599A (de)
CA (1) CA2330252A1 (de)
GB (1) GB9812674D0 (de)
WO (1) WO1999064624A2 (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2351853A1 (de) * 2000-06-06 2011-08-03 Life Technologies Corporation Methode und Vorrichtungen für multiplex Amplifizierungsreaktionen
US6605451B1 (en) * 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
US7087414B2 (en) 2000-06-06 2006-08-08 Applera Corporation Methods and devices for multiplexing amplification reactions
US8323897B2 (en) 2002-12-04 2012-12-04 Applied Biosystems, Llc Multiplex amplification of polynucleotides
HU229967B1 (hu) 2011-12-20 2015-03-30 Kps Diagnosztika Zrt Eljárás töredezett nukleinsavak szekvenciájának meghatározására

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5023171A (en) * 1989-08-10 1991-06-11 Mayo Foundation For Medical Education And Research Method for gene splicing by overlap extension using the polymerase chain reaction
WO1992015678A1 (en) * 1991-03-01 1992-09-17 Stratagene Pcr generated dicistronic dna molecules for producing antibodies
US5707863A (en) * 1993-02-25 1998-01-13 General Hospital Corporation Tumor suppressor gene merlin
ATE361377T1 (de) * 1997-09-29 2007-05-15 Hope City Leistungstarke verknüpfung von nukleinsäuresegmenten

Also Published As

Publication number Publication date
GB9812674D0 (en) 1998-08-12
WO1999064624A2 (en) 1999-12-16
AU5377599A (en) 1999-12-30
EP1086250A2 (de) 2001-03-28
JP2002517258A (ja) 2002-06-18
WO1999064624A3 (en) 2000-09-14

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