CA2328427A1 - Synthase de polyketide narbonolide de recombinaison - Google Patents

Synthase de polyketide narbonolide de recombinaison Download PDF

Info

Publication number
CA2328427A1
CA2328427A1 CA002328427A CA2328427A CA2328427A1 CA 2328427 A1 CA2328427 A1 CA 2328427A1 CA 002328427 A CA002328427 A CA 002328427A CA 2328427 A CA2328427 A CA 2328427A CA 2328427 A1 CA2328427 A1 CA 2328427A1
Authority
CA
Canada
Prior art keywords
pks
narbonolide
gene
domain
recombinant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002328427A
Other languages
English (en)
Inventor
Gary Ashley
Melanie C. Betlach
Mary Betlach
Robert Mcdaniel
Li Tang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kosan Biosciences Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/141,908 external-priority patent/US6503741B1/en
Application filed by Individual filed Critical Individual
Publication of CA2328427A1 publication Critical patent/CA2328427A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

L'invention porte sur des composés d'ADN de recombinaison qui codent tout ou partie de la synthase de polyketide narbonolide et qui sont utilisés pour exprimer des gènes de la synthase de polyketides de recombinaison dans des cellules hôtes pour la production de narbonolide et de dérivés de narbonolide, et de polyketides qui sont utiles comme antibiotiques et comme intermédiaires dans la synthèse de composés ayant une valeur pharmaceutique.
CA002328427A 1998-05-28 1999-05-27 Synthase de polyketide narbonolide de recombinaison Abandoned CA2328427A1 (fr)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US8708098P 1998-05-28 1998-05-28
US60/087,080 1998-05-28
US09/141,908 1998-08-28
US09/141,908 US6503741B1 (en) 1998-05-28 1998-08-28 Polyketide synthase genes from Streptomyces venezuelae
US10088098P 1998-09-22 1998-09-22
US60/100,880 1998-09-22
US11913999P 1999-02-08 1999-02-08
US60/119,139 1999-02-08
PCT/US1999/011814 WO1999061599A2 (fr) 1998-05-28 1999-05-27 Synthase de polyketide narbonolide de recombinaison

Publications (1)

Publication Number Publication Date
CA2328427A1 true CA2328427A1 (fr) 1999-12-02

Family

ID=27492096

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002328427A Abandoned CA2328427A1 (fr) 1998-05-28 1999-05-27 Synthase de polyketide narbonolide de recombinaison

Country Status (6)

Country Link
EP (1) EP1082439A2 (fr)
JP (1) JP2002516090A (fr)
AU (1) AU762399C (fr)
CA (1) CA2328427A1 (fr)
NZ (1) NZ509006A (fr)
WO (1) WO1999061599A2 (fr)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6265202B1 (en) 1998-06-26 2001-07-24 Regents Of The University Of Minnesota DNA encoding methymycin and pikromycin
EP1754700A3 (fr) 1999-01-27 2007-05-09 Kosan Biosciences, Inc. synthese de polycetides
US7033818B2 (en) 1999-10-08 2006-04-25 Kosan Biosciences, Inc. Recombinant polyketide synthase genes
US6524841B1 (en) 1999-10-08 2003-02-25 Kosan Biosciences, Inc. Recombinant megalomicin biosynthetic genes and uses thereof
US6838265B2 (en) 2000-05-02 2005-01-04 Kosan Biosciences, Inc. Overproduction hosts for biosynthesis of polyketides
WO2002059322A2 (fr) * 2000-10-17 2002-08-01 Cubist Pharmaceuticlas, Inc. Compositions et methodes liees a la famille de genes biosynthetiques de la daptomycine
DE60330651D1 (en) 2002-03-12 2010-02-04 Bristol Myers Squibb Co C3-cyanoepothilonderivate
CN103459610A (zh) 2011-01-28 2013-12-18 阿迈瑞斯公司 凝胶包裹的微集落筛选
BR112013027954A2 (pt) 2011-05-13 2017-01-17 Amyris Inc métodos e composições para detectar a produção microbiana de compostos imiscíveis em água
CA2879178C (fr) 2012-08-07 2020-11-24 Amyris, Inc. Methodes de stabilisation de la production de composes derives de l'acetyl-coenzyme a
EP2971027B1 (fr) 2013-03-15 2019-01-30 Amyris, Inc. Utilisation de phosphocétolase et de phosphotransacétylase pour la production de composés dérivés d'acétyl-coenzyme a
BR112016002526B1 (pt) 2013-08-07 2021-11-23 Total Marketing Services Método para produção de um composto heterólogo não catabólico, e, composição de fermentação
EP3313996A1 (fr) 2015-06-25 2018-05-02 Amyris, Inc. Dégrons dépendant du maltose, promoteurs sensibles au maltose, constructions de stabilisation, et leur utilisation dans la production de composés non cataboliques

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5712146A (en) * 1993-09-20 1998-01-27 The Leland Stanford Junior University Recombinant combinatorial genetic library for the production of novel polyketides
TR199900006T2 (xx) * 1996-07-05 1999-04-21 Biotica Technology Limited Eritromisinler ve bunlar�n haz�rlanmas� i�in i�lem.

Also Published As

Publication number Publication date
WO1999061599A2 (fr) 1999-12-02
JP2002516090A (ja) 2002-06-04
AU762399B2 (en) 2003-06-26
AU762399C (en) 2004-02-05
EP1082439A2 (fr) 2001-03-14
AU4213799A (en) 1999-12-13
WO1999061599A3 (fr) 2000-01-27
NZ509006A (en) 2003-09-26

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued