CA2328212C - Gel electrophoresis apparatus having cam-activated clamp and methods of use - Google Patents
Gel electrophoresis apparatus having cam-activated clamp and methods of use Download PDFInfo
- Publication number
- CA2328212C CA2328212C CA002328212A CA2328212A CA2328212C CA 2328212 C CA2328212 C CA 2328212C CA 002328212 A CA002328212 A CA 002328212A CA 2328212 A CA2328212 A CA 2328212A CA 2328212 C CA2328212 C CA 2328212C
- Authority
- CA
- Canada
- Prior art keywords
- mounting block
- cam
- container
- gel cassette
- gel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000001502 gel electrophoresis Methods 0.000 title claims description 13
- 238000000034 method Methods 0.000 title claims description 11
- 239000000872 buffer Substances 0.000 claims abstract description 84
- 238000001962 electrophoresis Methods 0.000 claims abstract description 29
- 238000003780 insertion Methods 0.000 claims description 5
- 230000037431 insertion Effects 0.000 claims description 5
- 230000008878 coupling Effects 0.000 claims 2
- 238000010168 coupling process Methods 0.000 claims 2
- 238000005859 coupling reaction Methods 0.000 claims 2
- 239000000499 gel Substances 0.000 description 64
- 239000007853 buffer solution Substances 0.000 description 22
- 238000002955 isolation Methods 0.000 description 5
- 239000000523 sample Substances 0.000 description 4
- 238000001746 injection moulding Methods 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 108020004414 DNA Proteins 0.000 description 2
- 239000004593 Epoxy Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- AXAVXPMQTGXXJZ-UHFFFAOYSA-N 2-aminoacetic acid;2-amino-2-(hydroxymethyl)propane-1,3-diol Chemical compound NCC(O)=O.OCC(N)(CO)CO AXAVXPMQTGXXJZ-UHFFFAOYSA-N 0.000 description 1
- 229920002972 Acrylic fiber Polymers 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 229920004943 Delrin® Polymers 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 239000004020 conductor Substances 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 229920002477 rna polymer Polymers 0.000 description 1
- 239000012723 sample buffer Substances 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T74/00—Machine element or mechanism
- Y10T74/18—Mechanical movements
- Y10T74/18888—Reciprocating to or from oscillating
- Y10T74/1892—Lever and slide
- Y10T74/1896—Cam connections
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Electrochemistry (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Peptides Or Proteins (AREA)
- Eye Examination Apparatus (AREA)
- Reciprocating Pumps (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/059,604 | 1998-04-13 | ||
| US09/059,604 US6001233A (en) | 1998-04-13 | 1998-04-13 | Gel electrophoresis apparatus having CAM-activated clamp and methods of use |
| PCT/US1999/007976 WO1999053305A1 (en) | 1998-04-13 | 1999-04-12 | Gel electrophoresis apparatus having cam-activated clamp and methods of use |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2328212A1 CA2328212A1 (en) | 1999-10-21 |
| CA2328212C true CA2328212C (en) | 2007-08-07 |
Family
ID=22024054
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002328212A Expired - Fee Related CA2328212C (en) | 1998-04-13 | 1999-04-12 | Gel electrophoresis apparatus having cam-activated clamp and methods of use |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US6001233A (enExample) |
| EP (1) | EP1071947B1 (enExample) |
| JP (1) | JP4393703B2 (enExample) |
| AT (1) | ATE313791T1 (enExample) |
| AU (1) | AU3555199A (enExample) |
| CA (1) | CA2328212C (enExample) |
| DE (1) | DE69929044T2 (enExample) |
| DK (1) | DK1071947T3 (enExample) |
| WO (1) | WO1999053305A1 (enExample) |
Families Citing this family (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19854096A1 (de) * | 1998-11-24 | 2000-05-25 | Merck Patent Gmbh | Anschlußträger für plattenförmige Mikrokomponenten |
| US6162342A (en) * | 1999-02-12 | 2000-12-19 | Bio-Rad Laboratories, Inc. | Rapid assembly casting system for slab gels |
| US7517442B1 (en) | 1999-08-09 | 2009-04-14 | Life Technologies Corporation | Facile method and apparatus for the analysis of biological macromolecules in two dimensions using common and familiar electrophoresis formats |
| US6436262B1 (en) | 2000-04-24 | 2002-08-20 | Bio-Rad Laboratories, Inc. | Compact cell clamp for slab gel plate assembly |
| US6656700B2 (en) | 2000-05-26 | 2003-12-02 | Amersham Plc | Isoforms of human pregnancy-associated protein-E |
| US6686188B2 (en) | 2000-05-26 | 2004-02-03 | Amersham Plc | Polynucleotide encoding a human myosin-like polypeptide expressed predominantly in heart and muscle |
| US7601251B2 (en) * | 2001-05-10 | 2009-10-13 | Life Technologies Corporation | Methods and apparatus for low resistance electrophoresis of prior-cast, hydratable separation media |
| JP4236938B2 (ja) * | 2001-05-10 | 2009-03-11 | インビトロジェン コーポレイション | 予め注型された水和可能分離媒体を電気泳動するための方法および装置 |
| US20040078837A1 (en) * | 2001-08-02 | 2004-04-22 | Shannon Mark E. | Four human zinc-finger-containing proteins: MDZ3, MDZ4, MDZ7 and MDZ12 |
| US7749367B2 (en) * | 2003-03-05 | 2010-07-06 | Deming Zhou | Vertical slab gel electrophoresis cell and method therefor |
| US20050103628A1 (en) * | 2003-09-22 | 2005-05-19 | Jackson Thomas R. | Apparatus for concurrent electrophoresis in a plurality of gels |
| US7250097B2 (en) * | 2004-04-26 | 2007-07-31 | The United States Of America, Department Of Health And Human Services | Device for sequential protein transfer from a gel |
| WO2006113694A2 (en) * | 2005-04-15 | 2006-10-26 | Invitrogen Corporation | Gel cassette adapter |
| EP1872117B1 (en) * | 2005-04-15 | 2017-03-29 | Life Technologies Corporation | Expanding cam lock for sealing slab gels in an electrophoresis apparatus |
| CN2824030Y (zh) * | 2005-09-29 | 2006-10-04 | 北京百晶生物技术有限公司 | 一次装夹胶室的垂直电泳仪 |
| USD794823S1 (en) * | 2010-08-24 | 2017-08-15 | Life Technologies Corporation | Electrophoresis tank with a base and cassette inserted in |
| JP2014219218A (ja) * | 2013-05-01 | 2014-11-20 | システム・インスツルメンツ株式会社 | 電気泳動用ゲルホルダおよび電気泳動方法 |
| KR101470733B1 (ko) * | 2013-05-14 | 2014-12-08 | 고창욱 | 수직형 단백질 전기영동장치 시스템에 사용되는 누수방지 젤캐스팅장치 |
| USD816865S1 (en) | 2015-11-13 | 2018-05-01 | Life Technologies Corporation | Cassette support base for electrophoresis gel casting |
| USD859688S1 (en) | 2015-11-13 | 2019-09-10 | Life Technologies Corporation | Electrophoresis slab gel sample loading guide |
| USD849963S1 (en) | 2015-11-13 | 2019-05-28 | Life Technologies Corporation | Electrophoresis gel cassette |
| USD851779S1 (en) | 2015-11-13 | 2019-06-18 | Life Technologies Corporation | Electrophoresis cassette with sample loading guide |
| USD792603S1 (en) | 2015-11-13 | 2017-07-18 | Life Technologies Corporation | Rig for electrophoresis gel casting |
| USD856528S1 (en) | 2015-11-13 | 2019-08-13 | Life Technologies Corporation | Cassette clamp for electrophoresis gel casting |
| US10359396B2 (en) | 2015-11-13 | 2019-07-23 | Life Technologies Corporation | Preparation of electrophoresis gels, and related devices, systems, and methods |
| USD975873S1 (en) | 2020-07-13 | 2023-01-17 | Life Technologies Corporation | Electrophoresis and electrotransfer device |
| KR20230035659A (ko) * | 2020-07-13 | 2023-03-14 | 라이프 테크놀로지스 코포레이션 | 전기영동 및 전기이동 디바이스, 시스템, 및 방법 |
| KR102642983B1 (ko) * | 2021-06-16 | 2024-03-04 | 강규광 | 연구용 전기 영동장치 |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US344713A (en) * | 1886-06-29 | Clamp | ||
| CH293210A (it) * | 1951-09-12 | 1953-09-15 | Uffireco S A | Morsetto a mano senza vite di serraggio. |
| USD261609S (en) | 1979-05-02 | 1981-11-03 | Pierre Roux | Vise |
| US4399989A (en) * | 1981-05-13 | 1983-08-23 | Baillie Robert A | Safe grip slicer for bagels, rolls, muffins and the like |
| JPS62127662A (ja) * | 1985-11-29 | 1987-06-09 | Dai Ichi Pure Chem Co Ltd | タテ・スラブ型電気泳動装置 |
| US4929329A (en) * | 1987-04-27 | 1990-05-29 | Eg&G, Inc. | Electrophoresis cassette system with apparatus and method for filling same |
| US4872358A (en) * | 1987-06-11 | 1989-10-10 | U.S. Philips Corporation | Driving mechanism having a pressure member |
| US4884792A (en) * | 1988-08-11 | 1989-12-05 | Lake Center Industries A Division Of Guy F. Atkinson Company | Clamp |
| US5112470A (en) * | 1990-12-07 | 1992-05-12 | Stratagene | Electrophoresis apparatus |
| JPH0624768A (ja) * | 1992-07-01 | 1994-02-01 | Nippon Electric Glass Co Ltd | 天然大理石様結晶化ガラス物品及びその製造方法 |
| US5792332A (en) * | 1995-12-21 | 1998-08-11 | Hoefer Pharmacia Biotech, Inc. | Safe electrophoresis unit |
| US5632877A (en) * | 1996-05-20 | 1997-05-27 | Bio-Rad Laboratories, Inc. | Rapid assembly electrophoresis cell for slab gels |
| US5888369A (en) * | 1997-03-28 | 1999-03-30 | Novel Experimental Technology | Apparatus for conducting electrophoresis experiments |
-
1998
- 1998-04-13 US US09/059,604 patent/US6001233A/en not_active Expired - Lifetime
-
1999
- 1999-04-12 CA CA002328212A patent/CA2328212C/en not_active Expired - Fee Related
- 1999-04-12 DE DE69929044T patent/DE69929044T2/de not_active Expired - Lifetime
- 1999-04-12 EP EP99917424A patent/EP1071947B1/en not_active Expired - Lifetime
- 1999-04-12 JP JP2000543821A patent/JP4393703B2/ja not_active Expired - Lifetime
- 1999-04-12 WO PCT/US1999/007976 patent/WO1999053305A1/en not_active Ceased
- 1999-04-12 DK DK99917424T patent/DK1071947T3/da active
- 1999-04-12 AU AU35551/99A patent/AU3555199A/en not_active Abandoned
- 1999-04-12 AT AT99917424T patent/ATE313791T1/de not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| WO1999053305A1 (en) | 1999-10-21 |
| DE69929044T2 (de) | 2006-08-17 |
| EP1071947B1 (en) | 2005-12-21 |
| EP1071947A1 (en) | 2001-01-31 |
| JP4393703B2 (ja) | 2010-01-06 |
| DK1071947T3 (da) | 2006-03-06 |
| JP2002511584A (ja) | 2002-04-16 |
| CA2328212A1 (en) | 1999-10-21 |
| AU3555199A (en) | 1999-11-01 |
| ATE313791T1 (de) | 2006-01-15 |
| DE69929044D1 (de) | 2006-01-26 |
| US6001233A (en) | 1999-12-14 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |
Effective date: 20130412 |