CA2325571A1 - Promoteur et produits de synthese destines a l'expression de proteines de recombinaison dans des champignons filamenteux - Google Patents

Promoteur et produits de synthese destines a l'expression de proteines de recombinaison dans des champignons filamenteux Download PDF

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Publication number
CA2325571A1
CA2325571A1 CA002325571A CA2325571A CA2325571A1 CA 2325571 A1 CA2325571 A1 CA 2325571A1 CA 002325571 A CA002325571 A CA 002325571A CA 2325571 A CA2325571 A CA 2325571A CA 2325571 A1 CA2325571 A1 CA 2325571A1
Authority
CA
Canada
Prior art keywords
aspergillus
dna
promoter
sequence
gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002325571A
Other languages
English (en)
Inventor
Heidi Sisniega Barroso
Francisco Javier Casqueiro Blanco
Francisco Jose Moralejo Lorenzo
Juan Francisco Martin Martin
Santiago Gutierrez Martin
Maria Jose Hijarrubia Ibrahim
Jose Luis Del Rio Pericacho
Ignacio Faus Santasusana
Rosa Elena Cardoza Silva
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Urquima SA
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2325571A1 publication Critical patent/CA2325571A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0016—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with NAD or NADP as acceptor (1.4.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C07K14/43—Sweetening agents, e.g. thaumatin, monellin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Abstract

L'invention concerne de nouveaux promoteurs des gènes de glutamate déshydrogénase (gdh) provenant d'Aspergillus awamori et d'aspergilli qui lui sont associés, ainsi que de nouvelles séquences d'ADN codant des glutamate déshydrogénases provenant d'Aspergillus awamori. L'invention concerne également l'utilisation des promoteurs des gènes de gdh provenant d'un champignon du genre Aspergillus pour l'expression de protéines de recombinaison dans des champignons filamenteux.
CA002325571A 1998-04-02 1999-04-01 Promoteur et produits de synthese destines a l'expression de proteines de recombinaison dans des champignons filamenteux Abandoned CA2325571A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
ES9800699A ES2143408B1 (es) 1998-04-02 1998-04-02 Promotor y construcciones para expresion de proteinas recombinantes en hongos filamentosos.
ESP9800699 1998-04-02
PCT/EP1999/002243 WO1999051756A2 (fr) 1998-04-02 1999-04-01 Promoteur et produits de synthese destines a l'expression de proteines de recombinaison dans des champignons filamenteux

Publications (1)

Publication Number Publication Date
CA2325571A1 true CA2325571A1 (fr) 1999-10-14

Family

ID=8303317

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002325571A Abandoned CA2325571A1 (fr) 1998-04-02 1999-04-01 Promoteur et produits de synthese destines a l'expression de proteines de recombinaison dans des champignons filamenteux

Country Status (6)

Country Link
EP (1) EP1084262A1 (fr)
JP (1) JP2002510497A (fr)
AU (1) AU4257599A (fr)
CA (1) CA2325571A1 (fr)
ES (1) ES2143408B1 (fr)
WO (1) WO1999051756A2 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7883872B2 (en) 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
CA2345356C (fr) 1998-10-06 2012-10-02 Mark Aaron Emalfarb Systeme de transformation dans le domaine des hotes fongiques filamenteux
CN1137265C (zh) * 2000-07-06 2004-02-04 中国科学院微生物研究所 一种提高植物氮素同化效率的方法
EP1231272A3 (fr) * 2001-02-08 2002-11-13 Laboratorios Ovejero S.A. Procédé d'obtention de chymosine bovine (rennine)
WO2003070957A2 (fr) * 2002-02-20 2003-08-28 Novozymes A/S Production de polypeptides de plantes
DE102005035888A1 (de) * 2005-07-30 2007-02-01 Maltagen Forschung Gmbh Thaumatin aus transgenen monokotylen Pflanzen
EP2505651A3 (fr) 2006-12-10 2013-01-09 Dyadic International, Inc. Isolat de champignon avec activité protéase réduite
US8551751B2 (en) 2007-09-07 2013-10-08 Dyadic International, Inc. BX11 enzymes having xylosidase activity
CN103748226A (zh) * 2011-08-24 2014-04-23 诺维信股份有限公司 获得丝状真菌宿主细胞的阳性转化体的方法
EP2748322A2 (fr) 2011-08-24 2014-07-02 Novozymes, Inc. Procédés de production de multiples polypeptides recombinants dans une cellule hôte fongique filamenteuse

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI872102A (fi) * 1986-06-06 1987-12-07 Panlabs Inc Expressionssystem hos filamentala fungi.
ES2080689B1 (es) * 1994-04-21 1996-09-01 Urquima Sa Procedimiento de obtencion de un edulcorante natural proteico.
DE69631127T2 (de) * 1995-05-18 2004-07-08 Genencor International, Inc., Palo Alto Glykosyltransferase expression in aspergillus
ES2127697B1 (es) * 1997-03-05 2000-03-16 Antibioticos Sau Promotores de los genes glutamato deshidrogenasa, beta-n-acetilhexosaminidasa y gamma-actina y su utilizacion en sistemas de expresion, secrecion y antisentido de hongos filamentosos.

Also Published As

Publication number Publication date
AU4257599A (en) 1999-10-25
WO1999051756A3 (fr) 2001-01-18
WO1999051756A9 (fr) 2000-02-17
ES2143408B1 (es) 2000-12-01
EP1084262A1 (fr) 2001-03-21
ES2143408A1 (es) 2000-05-01
WO1999051756A2 (fr) 1999-10-14
JP2002510497A (ja) 2002-04-09

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued