CA2322975A1 - Procedes de purification et de detection par electrophorese d'affinite reversible - Google Patents

Procedes de purification et de detection par electrophorese d'affinite reversible Download PDF

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Publication number
CA2322975A1
CA2322975A1 CA002322975A CA2322975A CA2322975A1 CA 2322975 A1 CA2322975 A1 CA 2322975A1 CA 002322975 A CA002322975 A CA 002322975A CA 2322975 A CA2322975 A CA 2322975A CA 2322975 A1 CA2322975 A1 CA 2322975A1
Authority
CA
Canada
Prior art keywords
analyte
electrophoretic medium
varied
electric field
affinity ligand
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002322975A
Other languages
English (en)
Inventor
Ezra S. Abrams
Philip W. Hammond
Andrew R. Muir
T. Christian Boles
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mosaic Technologies Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2322975A1 publication Critical patent/CA2322975A1/fr
Abandoned legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Electrochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé d'électrophorèse d'affinité dans lequel on fait varier le sens de l'électrophorèse de manière cyclique tout en changeant de manière synchronisée une ou plusieurs propriétés du milieu électrophorétique entre deux états, lesdits états étant caractérisés comme favorisant ou défavorisant la liaison réversible spécifique de substances à analyser d'échantillons à des ligands d'affinité immobilisés dans le milieu. Le procédé obtenu permet une séparation extrêmement efficace et pratique des substances à analyser spécifiques à détecter ou à purifier, avec des matériels et un appareil simples.
CA002322975A 1998-03-03 1999-03-03 Procedes de purification et de detection par electrophorese d'affinite reversible Abandoned CA2322975A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US7661498P 1998-03-03 1998-03-03
US60/076,614 1998-03-03
PCT/US1999/004849 WO1999045374A2 (fr) 1998-03-03 1999-03-03 Procedes de purification et de detection par electrophorese d'affinite reversible

Publications (1)

Publication Number Publication Date
CA2322975A1 true CA2322975A1 (fr) 1999-09-10

Family

ID=22133151

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002322975A Abandoned CA2322975A1 (fr) 1998-03-03 1999-03-03 Procedes de purification et de detection par electrophorese d'affinite reversible

Country Status (5)

Country Link
EP (1) EP1068518A2 (fr)
JP (1) JP2002506204A (fr)
AU (1) AU2896399A (fr)
CA (1) CA2322975A1 (fr)
WO (1) WO1999045374A2 (fr)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0981647B1 (fr) 1997-05-16 2006-08-09 EXACT Sciences Corporation Analyse electrophoretique de molecules au moyen de sondes immobilisees
US6586177B1 (en) 1999-09-08 2003-07-01 Exact Sciences Corporation Methods for disease detection
FR2799988B1 (fr) 1999-10-26 2001-12-07 Centre Nat Rech Scient Procede de separation d'un compose chimique ou biologique dans un melange de composes similaires par diffusion dans un milieu tel qu'un gel
FR2806161B1 (fr) 2000-03-10 2002-09-20 Sebia Sa Methode de separation, d'identification et de quantification des isophosphatases alcalines par electrophorese
JP4598989B2 (ja) * 2001-05-29 2010-12-15 アロカ株式会社 電気泳動装置
EP1685395A2 (fr) * 2003-11-05 2006-08-02 EXACT Sciences Corporation Techniques de separation repetitive par affinite et leurs utilisations
US8529744B2 (en) 2004-02-02 2013-09-10 Boreal Genomics Corp. Enrichment of nucleic acid targets
US8518228B2 (en) 2011-05-20 2013-08-27 The University Of British Columbia Systems and methods for enhanced SCODA
US9186685B2 (en) 2012-01-13 2015-11-17 The University Of British Columbia Multiple arm apparatus and methods for separation of particles
CA2552262C (fr) 2004-02-02 2016-04-19 The University Of British Columbia Scodaphorese, procedes et appareil utilises pour deplacer et concentrer des particules
US10337054B2 (en) 2004-02-02 2019-07-02 Quantum-Si Incorporated Enrichment of nucleic acid targets
US7668235B2 (en) * 2005-11-10 2010-02-23 Teradyne Jitter measurement algorithm using locally in-order strobes
CN101535800B (zh) 2006-08-29 2013-07-17 贝克顿迪肯森公司 用于无载体偏转电泳的设备及方法
EP3656403B1 (fr) 2007-08-17 2022-05-11 Purdue Research Foundation Procédés de préparation de conjugués ligand-lieur de liaison au psma
US8475641B2 (en) 2008-02-01 2013-07-02 The University Of British Columbia Methods and apparatus for particle introduction and recovery
WO2010121381A1 (fr) 2009-04-21 2010-10-28 The University Of British Columbia Système et procédés pour la détection de particules
US9951324B2 (en) 2010-02-25 2018-04-24 Purdue Research Foundation PSMA binding ligand-linker conjugates and methods for using
WO2013166444A2 (fr) 2012-05-04 2013-11-07 Boreal Genomics Corp. Analyse de biomarqueurs utilisant la scodaphorèse
JP6892218B2 (ja) 2012-11-15 2021-06-23 エンドサイト・インコーポレイテッドＥｎｄｏｃｙｔｅ， Ｉｎｃ． 薬物送達結合体およびｐｓｍａ発現細胞によって引き起こされる疾患の治療方法
US9340835B2 (en) 2013-03-15 2016-05-17 Boreal Genomics Corp. Method for separating homoduplexed and heteroduplexed nucleic acids
GEP20237496B (en) 2013-10-18 2023-04-10 Deutsches Krebsforsch Labeled inhibitors of prostate specific membrane antigen (psma), their use as imaging agents and pharmaceutical agents for the treatment of prostate cancer
US10188759B2 (en) 2015-01-07 2019-01-29 Endocyte, Inc. Conjugates for imaging
US11130986B2 (en) 2015-05-20 2021-09-28 Quantum-Si Incorporated Method for isolating target nucleic acid using heteroduplex binding proteins

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5167790A (en) * 1985-09-27 1992-12-01 Washington University Field-inversion gel electrophoresis
GB9502660D0 (en) * 1995-02-10 1995-03-29 Univ London Detection of mutations or variations in DNA
WO1997045721A1 (fr) * 1996-05-24 1997-12-04 Novartis Ag Procede pour separer des melanges de substances au moyen d'une lectrophorese sur gel par affinite capillaire

Also Published As

Publication number Publication date
WO1999045374A2 (fr) 1999-09-10
AU2896399A (en) 1999-09-20
EP1068518A2 (fr) 2001-01-17
JP2002506204A (ja) 2002-02-26
WO1999045374A3 (fr) 2000-02-17

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Legal Events

Date Code Title Description
FZDE Discontinued