WO1999045374A3 - Procedes de purification et de detection par electrophorese d'affinite reversible - Google Patents

Procedes de purification et de detection par electrophorese d'affinite reversible Download PDF

Info

Publication number
WO1999045374A3
WO1999045374A3 PCT/US1999/004849 US9904849W WO9945374A3 WO 1999045374 A3 WO1999045374 A3 WO 1999045374A3 US 9904849 W US9904849 W US 9904849W WO 9945374 A3 WO9945374 A3 WO 9945374A3
Authority
WO
WIPO (PCT)
Prior art keywords
purification
electrophoresis
detection processes
affinity electrophoresis
states
Prior art date
Application number
PCT/US1999/004849
Other languages
English (en)
Other versions
WO1999045374A2 (fr
Inventor
Ezra S Abrams
Philip W Hammond
Andrew R Muir
T Christian Boles
Original Assignee
Mosaic Technologies
Ezra S Abrams
Philip W Hammond
Andrew R Muir
T Christian Boles
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mosaic Technologies, Ezra S Abrams, Philip W Hammond, Andrew R Muir, T Christian Boles filed Critical Mosaic Technologies
Priority to EP99909853A priority Critical patent/EP1068518A2/fr
Priority to AU28963/99A priority patent/AU2896399A/en
Priority to JP2000534862A priority patent/JP2002506204A/ja
Priority to CA002322975A priority patent/CA2322975A1/fr
Publication of WO1999045374A2 publication Critical patent/WO1999045374A2/fr
Publication of WO1999045374A3 publication Critical patent/WO1999045374A3/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis

Abstract

L'invention concerne un procédé d'électrophorèse d'affinité dans lequel on fait varier le sens de l'électrophorèse de manière cyclique tout en changeant de manière synchronisée une ou plusieurs propriétés du milieu électrophorétique entre deux états, lesdits états étant caractérisés comme favorisant ou défavorisant la liaison réversible spécifique de substances à analyser d'échantillons à des ligands d'affinité immobilisés dans le milieu. Le procédé obtenu permet une séparation extrêmement efficace et pratique des substances à analyser spécifiques à détecter ou à purifier, avec des matériels et un appareil simples.
PCT/US1999/004849 1998-03-03 1999-03-03 Procedes de purification et de detection par electrophorese d'affinite reversible WO1999045374A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP99909853A EP1068518A2 (fr) 1998-03-03 1999-03-03 Procedes de purification et de detection par electrophorese d'affinite reversible
AU28963/99A AU2896399A (en) 1998-03-03 1999-03-03 Purification and detection processes using reversible affinity electrophoresis
JP2000534862A JP2002506204A (ja) 1998-03-03 1999-03-03 可逆的アフィニティー電気泳動を用いる精製および検出プロセス
CA002322975A CA2322975A1 (fr) 1998-03-03 1999-03-03 Procedes de purification et de detection par electrophorese d'affinite reversible

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7661498P 1998-03-03 1998-03-03
US60/076,614 1998-03-03

Publications (2)

Publication Number Publication Date
WO1999045374A2 WO1999045374A2 (fr) 1999-09-10
WO1999045374A3 true WO1999045374A3 (fr) 2000-02-17

Family

ID=22133151

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/004849 WO1999045374A2 (fr) 1998-03-03 1999-03-03 Procedes de purification et de detection par electrophorese d'affinite reversible

Country Status (5)

Country Link
EP (1) EP1068518A2 (fr)
JP (1) JP2002506204A (fr)
AU (1) AU2896399A (fr)
CA (1) CA2322975A1 (fr)
WO (1) WO1999045374A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8907058B2 (en) 2007-08-17 2014-12-09 Purdue Research Foundation PSMA binding ligand-linker conjugates and methods for using

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69835516D1 (de) 1997-05-16 2006-09-21 Exact Sciences Corp Elektrophoretische analyse von molekülen mit immobilisierten sonden
US6586177B1 (en) 1999-09-08 2003-07-01 Exact Sciences Corporation Methods for disease detection
FR2799988B1 (fr) * 1999-10-26 2001-12-07 Centre Nat Rech Scient Procede de separation d'un compose chimique ou biologique dans un melange de composes similaires par diffusion dans un milieu tel qu'un gel
FR2806161B1 (fr) 2000-03-10 2002-09-20 Sebia Sa Methode de separation, d'identification et de quantification des isophosphatases alcalines par electrophorese
JP4598989B2 (ja) * 2001-05-29 2010-12-15 アロカ株式会社 電気泳動装置
US7767468B2 (en) 2003-11-05 2010-08-03 Exact Sciences Corporation Repetitive affinity separation and uses therefor
US8529744B2 (en) 2004-02-02 2013-09-10 Boreal Genomics Corp. Enrichment of nucleic acid targets
US10337054B2 (en) 2004-02-02 2019-07-02 Quantum-Si Incorporated Enrichment of nucleic acid targets
JP2007526823A (ja) 2004-02-02 2007-09-20 ザ ユニバーシティ オブ ブリティッシュ コロンビア 粒子を運動および濃縮させるためのスコダ泳動ならびに方法および装置
US7668235B2 (en) * 2005-11-10 2010-02-23 Teradyne Jitter measurement algorithm using locally in-order strobes
CA2661268C (fr) * 2006-08-29 2016-09-13 Becton, Dickinson & Company Procede et dispositif d'electrophorese libre sans vecteur
WO2009094772A1 (fr) 2008-02-01 2009-08-06 The University Of British Columbia Procédés et appareil pour l'introduction et la récupération de particules
WO2010121381A1 (fr) 2009-04-21 2010-10-28 The University Of British Columbia Système et procédés pour la détection de particules
US9951324B2 (en) 2010-02-25 2018-04-24 Purdue Research Foundation PSMA binding ligand-linker conjugates and methods for using
CA2742327A1 (fr) 2011-05-20 2012-11-20 The University Of British Columiba Systemes et methodes pour amelioration de la scoda (separation, concentration, detection de molecules selon la difference d'affinite de liaison)
WO2013104994A2 (fr) 2012-01-13 2013-07-18 The University Of British Columbia Appareil à bras multiples et procédés pour la séparation de particules
WO2013166444A2 (fr) 2012-05-04 2013-11-07 Boreal Genomics Corp. Analyse de biomarqueurs utilisant la scodaphorèse
WO2014078484A1 (fr) 2012-11-15 2014-05-22 Endocyte, Inc. Conjugués pour traiter les maladies provoquées par des cellules exprimant psma
US9340835B2 (en) 2013-03-15 2016-05-17 Boreal Genomics Corp. Method for separating homoduplexed and heteroduplexed nucleic acids
MY194484A (en) 2013-10-18 2022-11-30 Deutsches Krebsforsch Labeled Inhibitors of Prostate Specific Membrane Antigen (PSMA), Their use as Imaging Agents and Pharmaceutical Agents for the Treatment of Prostate Cancer
US10188759B2 (en) 2015-01-07 2019-01-29 Endocyte, Inc. Conjugates for imaging
US11130986B2 (en) 2015-05-20 2021-09-28 Quantum-Si Incorporated Method for isolating target nucleic acid using heteroduplex binding proteins

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5167790A (en) * 1985-09-27 1992-12-01 Washington University Field-inversion gel electrophoresis
WO1996024687A1 (fr) * 1995-02-10 1996-08-15 University College London Detection de mutations ou de modifications dans l'adn
WO1997045721A1 (fr) * 1996-05-24 1997-12-04 Novartis Ag Procede pour separer des melanges de substances au moyen d'une lectrophorese sur gel par affinite capillaire

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5167790A (en) * 1985-09-27 1992-12-01 Washington University Field-inversion gel electrophoresis
WO1996024687A1 (fr) * 1995-02-10 1996-08-15 University College London Detection de mutations ou de modifications dans l'adn
WO1997045721A1 (fr) * 1996-05-24 1997-12-04 Novartis Ag Procede pour separer des melanges de substances au moyen d'une lectrophorese sur gel par affinite capillaire

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
"BioDirectory Catalogue", 1997, PHARMACIA BIOTECH, XP002124683 *
PENNER ET AL.: "Increased detection of polymorphism among randomly amplified wheat DNA frgaments using a modified temperature sweep gel electrophoresis technique", NUCLEIC ACIDS RESEARCH, vol. 22, no. 9, 11 May 1994 (1994-05-11), pages 1780 - 1781, XP002124682 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8907058B2 (en) 2007-08-17 2014-12-09 Purdue Research Foundation PSMA binding ligand-linker conjugates and methods for using
US9193763B2 (en) 2007-08-17 2015-11-24 Purdue Research Foundation PSMA binding ligand-linker conjugates and methods for using

Also Published As

Publication number Publication date
JP2002506204A (ja) 2002-02-26
EP1068518A2 (fr) 2001-01-17
WO1999045374A2 (fr) 1999-09-10
CA2322975A1 (fr) 1999-09-10
AU2896399A (en) 1999-09-20

Similar Documents

Publication Publication Date Title
WO1999045374A3 (fr) Procedes de purification et de detection par electrophorese d'affinite reversible
WO1998035232A3 (fr) Sondes electrochimiques pour la detection d'interactions moleculaires et la mise au point de medicaments
DE69526747D1 (de) Elektrobiochemisches Verfahren, System und Elektroden zur Bestimmung eines Mitgliedes eines Bindungspaares
CA2262920A1 (fr) Procede servant a identifier des composes exercant un effet sur les interactions de proteines de fixation d'arn et d'adn
CA2143999A1 (fr) Dispositif et methode pour essai de bioaffinite et separation par electrophorese combines
AU1400099A (en) High-throughput method and apparatus for identifying, quantitating and determining the purity of chemical compounds in mixtures
EP0691148A3 (fr)
ID30111A (id) LEMBARAN ATAS YANG DAPAT DILEWATI CAIRAN UNTUK BAHAN PENYERAP KOTORAN TUBUH, APARATUS DAN METODE PEMBUATANNYA (Pecahan dari NO. P970605)
Kato et al. Rapid analysis of amino acids in Japanese green tea by microchip electrophoresis using plastic microchip and fluorescence detection
AU5438796A (en) Method for rapid purification, analysis and characterization of collections of chemical compounds
AU3436695A (en) Reagent for analyzing solid components in urine and method for analyzing solid components by employing the same
WO1999016781A3 (fr) Procede de preparation d'echantillons automatisable, a usage universel
AU8112898A (en) Method for identifying and/or analysing biological substances, present in a conductive liquid, device and affinity sensor used for implementing said method
AU1847701A (en) Apparatus and method for trapping bead based reagents within microfluidic analysis systems
CA2322465A1 (fr) Puce a electrophorese capillaire fabriquee par micro-usinage et procede de detection simultanee de multiples marqueurs d'oxydo-reduction
EP0747703A3 (fr) Méthode pour l'analyse de substances avec l'activité biologiques
AU5964494A (en) Assays and kits for detecting analytes in the presence of cross-reacting substances
AU1957097A (en) Protein separations by capillary electrophoresis using amino acid-containing buffers
AU3028797A (en) Process for separating mixtures of substances using capillary affinity gel electrophoresis
AU6913196A (en) Analytical rotor and method for detecting analytes in liquid samples
WO2001055721A3 (fr) Detection de biomolecules dans des gels suite a une electrophorese
CA2294749A1 (fr) Dispositif et procede de concentration et purification de molecules biologiques en une etape
EP1652918A3 (fr) Methodes de detection d'interactions entre proteines utilisant des peptides a conformation contrainte
ZA961704B (fr)
Hogan et al. Indirect fluorometric detection of tryptic digests separated by capillary zone electrophoresis

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG US UZ VN YU ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW SD SL SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG US UZ VN YU ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW SD SL SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

NENP Non-entry into the national phase

Ref country code: KR

ENP Entry into the national phase

Ref country code: JP

Ref document number: 2000 534862

Kind code of ref document: A

Format of ref document f/p: F

ENP Entry into the national phase

Ref document number: 2322975

Country of ref document: CA

Ref country code: CA

Ref document number: 2322975

Kind code of ref document: A

Format of ref document f/p: F

WWE Wipo information: entry into national phase

Ref document number: 28963/99

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 1999909853

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWP Wipo information: published in national office

Ref document number: 1999909853

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 1999909853

Country of ref document: EP