WO1999045374A3 - Procedes de purification et de detection par electrophorese d'affinite reversible - Google Patents

Procedes de purification et de detection par electrophorese d'affinite reversible Download PDF

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Publication number
WO1999045374A3
WO1999045374A3 PCT/US1999/004849 US9904849W WO9945374A3 WO 1999045374 A3 WO1999045374 A3 WO 1999045374A3 US 9904849 W US9904849 W US 9904849W WO 9945374 A3 WO9945374 A3 WO 9945374A3
Authority
WO
WIPO (PCT)
Prior art keywords
purification
electrophoresis
detection processes
affinity electrophoresis
states
Prior art date
Application number
PCT/US1999/004849
Other languages
English (en)
Other versions
WO1999045374A2 (fr
Inventor
Ezra S Abrams
Philip W Hammond
Andrew R Muir
T Christian Boles
Original Assignee
Mosaic Technologies
Ezra S Abrams
Philip W Hammond
Andrew R Muir
T Christian Boles
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mosaic Technologies, Ezra S Abrams, Philip W Hammond, Andrew R Muir, T Christian Boles filed Critical Mosaic Technologies
Priority to EP99909853A priority Critical patent/EP1068518A2/fr
Priority to CA002322975A priority patent/CA2322975A1/fr
Priority to AU28963/99A priority patent/AU2896399A/en
Priority to JP2000534862A priority patent/JP2002506204A/ja
Publication of WO1999045374A2 publication Critical patent/WO1999045374A2/fr
Publication of WO1999045374A3 publication Critical patent/WO1999045374A3/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Electrochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé d'électrophorèse d'affinité dans lequel on fait varier le sens de l'électrophorèse de manière cyclique tout en changeant de manière synchronisée une ou plusieurs propriétés du milieu électrophorétique entre deux états, lesdits états étant caractérisés comme favorisant ou défavorisant la liaison réversible spécifique de substances à analyser d'échantillons à des ligands d'affinité immobilisés dans le milieu. Le procédé obtenu permet une séparation extrêmement efficace et pratique des substances à analyser spécifiques à détecter ou à purifier, avec des matériels et un appareil simples.
PCT/US1999/004849 1998-03-03 1999-03-03 Procedes de purification et de detection par electrophorese d'affinite reversible WO1999045374A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP99909853A EP1068518A2 (fr) 1998-03-03 1999-03-03 Procedes de purification et de detection par electrophorese d'affinite reversible
CA002322975A CA2322975A1 (fr) 1998-03-03 1999-03-03 Procedes de purification et de detection par electrophorese d'affinite reversible
AU28963/99A AU2896399A (en) 1998-03-03 1999-03-03 Purification and detection processes using reversible affinity electrophoresis
JP2000534862A JP2002506204A (ja) 1998-03-03 1999-03-03 可逆的アフィニティー電気泳動を用いる精製および検出プロセス

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7661498P 1998-03-03 1998-03-03
US60/076,614 1998-03-03

Publications (2)

Publication Number Publication Date
WO1999045374A2 WO1999045374A2 (fr) 1999-09-10
WO1999045374A3 true WO1999045374A3 (fr) 2000-02-17

Family

ID=22133151

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/004849 WO1999045374A2 (fr) 1998-03-03 1999-03-03 Procedes de purification et de detection par electrophorese d'affinite reversible

Country Status (5)

Country Link
EP (1) EP1068518A2 (fr)
JP (1) JP2002506204A (fr)
AU (1) AU2896399A (fr)
CA (1) CA2322975A1 (fr)
WO (1) WO1999045374A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8907058B2 (en) 2007-08-17 2014-12-09 Purdue Research Foundation PSMA binding ligand-linker conjugates and methods for using

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU730491B2 (en) 1997-05-16 2001-03-08 Exact Sciences Corporation Electrophoretic analysis of molecules using immobilized probes
US6586177B1 (en) 1999-09-08 2003-07-01 Exact Sciences Corporation Methods for disease detection
FR2799988B1 (fr) 1999-10-26 2001-12-07 Centre Nat Rech Scient Procede de separation d'un compose chimique ou biologique dans un melange de composes similaires par diffusion dans un milieu tel qu'un gel
FR2806161B1 (fr) * 2000-03-10 2002-09-20 Sebia Sa Methode de separation, d'identification et de quantification des isophosphatases alcalines par electrophorese
JP4598989B2 (ja) * 2001-05-29 2010-12-15 アロカ株式会社 電気泳動装置
EP1685395A2 (fr) 2003-11-05 2006-08-02 EXACT Sciences Corporation Techniques de separation repetitive par affinite et leurs utilisations
CA2552262C (fr) 2004-02-02 2016-04-19 The University Of British Columbia Scodaphorese, procedes et appareil utilises pour deplacer et concentrer des particules
US10337054B2 (en) 2004-02-02 2019-07-02 Quantum-Si Incorporated Enrichment of nucleic acid targets
US9186685B2 (en) 2012-01-13 2015-11-17 The University Of British Columbia Multiple arm apparatus and methods for separation of particles
US8529744B2 (en) 2004-02-02 2013-09-10 Boreal Genomics Corp. Enrichment of nucleic acid targets
US7668235B2 (en) * 2005-11-10 2010-02-23 Teradyne Jitter measurement algorithm using locally in-order strobes
US20080110758A1 (en) * 2006-08-29 2008-05-15 Becton, Dickinson And Company Methods and apparatus for carrier-free deflection electrophoresis
US8475641B2 (en) 2008-02-01 2013-07-02 The University Of British Columbia Methods and apparatus for particle introduction and recovery
US8877028B2 (en) 2009-04-21 2014-11-04 The University Of British Columbia System and methods for detection of particles
US9951324B2 (en) 2010-02-25 2018-04-24 Purdue Research Foundation PSMA binding ligand-linker conjugates and methods for using
CA2742327A1 (fr) 2011-05-20 2012-11-20 The University Of British Columiba Systemes et methodes pour amelioration de la scoda (separation, concentration, detection de molecules selon la difference d'affinite de liaison)
EP2844773B1 (fr) 2012-05-04 2017-08-16 Boreal Genomics Corp. Analyse de biomarqueurs utilisant la scodaphorèse
CA3158675A1 (fr) 2012-11-15 2014-05-22 Endocyte, Inc. Conjugues pour traiter les maladies provoquees par des cellules exprimant psma
US9340835B2 (en) 2013-03-15 2016-05-17 Boreal Genomics Corp. Method for separating homoduplexed and heteroduplexed nucleic acids
EP4095130B1 (fr) 2013-10-18 2024-01-31 Novartis AG Inhibiteurs marqués de l'antigène membranaire spécifique de la prostate (psma), leur utilisation comme agents d'imagerie et agents pharmaceutiques pour le traitement du cancer de la prostate
US10188759B2 (en) 2015-01-07 2019-01-29 Endocyte, Inc. Conjugates for imaging
US11130986B2 (en) 2015-05-20 2021-09-28 Quantum-Si Incorporated Method for isolating target nucleic acid using heteroduplex binding proteins
BR112022008098A2 (pt) 2019-10-29 2022-07-12 Quantum Si Inc Bombeamento peristáltico de fluidos e métodos, sistemas e dispositivos associados

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5167790A (en) * 1985-09-27 1992-12-01 Washington University Field-inversion gel electrophoresis
WO1996024687A1 (fr) * 1995-02-10 1996-08-15 University College London Detection de mutations ou de modifications dans l'adn
WO1997045721A1 (fr) * 1996-05-24 1997-12-04 Novartis Ag Procede pour separer des melanges de substances au moyen d'une lectrophorese sur gel par affinite capillaire

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5167790A (en) * 1985-09-27 1992-12-01 Washington University Field-inversion gel electrophoresis
WO1996024687A1 (fr) * 1995-02-10 1996-08-15 University College London Detection de mutations ou de modifications dans l'adn
WO1997045721A1 (fr) * 1996-05-24 1997-12-04 Novartis Ag Procede pour separer des melanges de substances au moyen d'une lectrophorese sur gel par affinite capillaire

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
"BioDirectory Catalogue", 1997, PHARMACIA BIOTECH, XP002124683 *
PENNER ET AL.: "Increased detection of polymorphism among randomly amplified wheat DNA frgaments using a modified temperature sweep gel electrophoresis technique", NUCLEIC ACIDS RESEARCH, vol. 22, no. 9, 11 May 1994 (1994-05-11), pages 1780 - 1781, XP002124682 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8907058B2 (en) 2007-08-17 2014-12-09 Purdue Research Foundation PSMA binding ligand-linker conjugates and methods for using
US9193763B2 (en) 2007-08-17 2015-11-24 Purdue Research Foundation PSMA binding ligand-linker conjugates and methods for using

Also Published As

Publication number Publication date
AU2896399A (en) 1999-09-20
WO1999045374A2 (fr) 1999-09-10
CA2322975A1 (fr) 1999-09-10
EP1068518A2 (fr) 2001-01-17
JP2002506204A (ja) 2002-02-26

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