CA2319691C - Eluting reagents, methods and kits for isolating dna - Google Patents

Eluting reagents, methods and kits for isolating dna Download PDF

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Publication number
CA2319691C
CA2319691C CA2319691A CA2319691A CA2319691C CA 2319691 C CA2319691 C CA 2319691C CA 2319691 A CA2319691 A CA 2319691A CA 2319691 A CA2319691 A CA 2319691A CA 2319691 C CA2319691 C CA 2319691C
Authority
CA
Canada
Prior art keywords
dna
solid support
reagent
cells
eluting
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA2319691A
Other languages
English (en)
French (fr)
Other versions
CA2319691A1 (en
Inventor
Ellen M. Heath
Ruth M. Shuman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qiagen North American Holdings Inc
Original Assignee
Qiagen North American Holdings Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen North American Holdings Inc filed Critical Qiagen North American Holdings Inc
Publication of CA2319691A1 publication Critical patent/CA2319691A1/en
Application granted granted Critical
Publication of CA2319691C publication Critical patent/CA2319691C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA2319691A 1998-02-02 1999-02-02 Eluting reagents, methods and kits for isolating dna Expired - Lifetime CA2319691C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US12642998P 1998-02-02 1998-02-02
US12641398P 1998-02-02 1998-02-02
US60/126,429 1998-02-02
US60/126,413 1998-02-02
PCT/US1999/002190 WO1999039010A1 (en) 1998-02-02 1999-02-02 Eluting reagents, methods and kits for isolating dna

Publications (2)

Publication Number Publication Date
CA2319691A1 CA2319691A1 (en) 1999-08-05
CA2319691C true CA2319691C (en) 2012-12-18

Family

ID=26824623

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2319691A Expired - Lifetime CA2319691C (en) 1998-02-02 1999-02-02 Eluting reagents, methods and kits for isolating dna

Country Status (5)

Country Link
EP (1) EP1053353A1 (ja)
JP (2) JP2004500002A (ja)
AU (1) AU2574299A (ja)
CA (1) CA2319691C (ja)
WO (1) WO1999039010A1 (ja)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2304017C (en) 1997-09-17 2007-05-01 Gentra Systems, Inc. Apparatuses and methods for isolating nucleic acid
US6750059B1 (en) 1998-07-16 2004-06-15 Whatman, Inc. Archiving of vectors
EP1141234A4 (en) * 1999-01-06 2004-05-19 Invitrogen Corp METHOD AND COMPOSITIONS FOR INSULATING NUCLEIC ACID MOLECULES
WO2002005930A1 (en) * 2000-07-18 2002-01-24 Invitrogen Corporation Device and methods for subdividing and filtering gel material and extracting molecules therefrom
US6803200B2 (en) 2000-12-12 2004-10-12 Invitrogen Corporation Compositions and methods for the release of nucleic acid molecules from solid matrices
US20050032105A1 (en) * 2001-10-12 2005-02-10 Bair Robert Jackson Compositions and methods for using a solid support to purify DNA
EP1466018B2 (en) 2002-01-08 2015-08-12 Roche Diagnostics GmbH Use of silica material in an amplification reaction
US20060094015A1 (en) * 2002-10-04 2006-05-04 Whatman Inc. Methods and materials for using chemical compounds as a tool for nucleic acid storage on media of nucleic acid purification systems
JP2008220380A (ja) * 2003-10-31 2008-09-25 Fujifilm Corp 核酸の分離精製方法
AU2005305012C1 (en) * 2004-11-05 2012-07-19 Qiagen North American Holdings, Inc. Compositions and methods for purifying nucleic acids from stabilization reagents
US9243289B2 (en) * 2013-12-23 2016-01-26 Roche Molecular Systems, Inc. Method for screening reagents used in PCR assays
AU2015342907A1 (en) * 2014-11-07 2017-05-25 The Johns Hopkins University Chaotrope- and volatile-free method for purifying nucleic acids from plasma
AU2016262545B2 (en) * 2015-05-12 2022-06-16 Gen-Probe Incorporated Method of pooling blood samples
AU2018234638B2 (en) 2017-03-15 2023-04-06 Ancestry.Com Dna, Llc Sample collection device and method

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL8900725A (nl) * 1989-03-23 1990-10-16 Az Univ Amsterdam Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur.
JPH04158784A (ja) * 1990-10-24 1992-06-01 Tax Adm Agency 酵母細胞壁溶解酵素の製造法及びその利用
DE4034036C2 (de) * 1990-10-26 1994-03-03 Diagen Inst Molekularbio Vorrichtung und Verfahren zur Isolierung von Nukleinsäuren aus Zellsuspensionen
US5654179A (en) * 1990-11-14 1997-08-05 Hri Research, Inc. Nucleic acid preparation methods
JPH06277040A (ja) * 1991-02-13 1994-10-04 Tax Adm Agency 酵母細胞壁溶解酵素の製造法及び溶解法
JP3294288B2 (ja) * 1991-02-22 2002-06-24 明治乳業株式会社 乳酸桿菌由来の新規なプラスミドｐＢＵＬ１ 及びその誘導体
CA2067712A1 (en) * 1991-05-03 1992-11-04 Daniel L. Woodard Filtration purification of dna
DE4139664A1 (de) * 1991-12-02 1993-06-03 Diagen Inst Molekularbio Vorrichtung und verfahren zur isolierung und reinigung von nukleinsaeuren
ATE184879T1 (de) * 1992-07-02 1999-10-15 Edge Biosystems Inc Verfahren und material für reinigung von nukleinsäuren
DE4321904B4 (de) * 1993-07-01 2013-05-16 Qiagen Gmbh Verfahren zur chromatographischen Reinigung und Trennung von Nucleinsäuregemischen
GB9314249D0 (en) * 1993-07-09 1993-08-18 Proofname Ltd Purification method and apparatus
US5438127A (en) * 1993-09-27 1995-08-01 Becton Dickinson And Company DNA purification by solid phase extraction using a PCl3 modified glass fiber membrane
DK0743950T3 (da) * 1994-02-11 2002-03-25 Qiagen Gmbh Fremgangsmåde til adskillelse af dobbeltstrengede/enkeltstrengede nukleinsyre-strukturer
JP3761573B2 (ja) * 1994-06-14 2006-03-29 インヴィテーク ゲーエムベーハー 極度に少量でかつ非常に強く汚染された非常に種々の出発物質から核酸を単離および精製する一般的方法
WO1996039813A1 (en) * 1995-06-07 1996-12-19 Flinders Technologies Pty. Ltd. Dry solid medium for storage and analysis of genetic material
US5702884A (en) * 1996-03-12 1997-12-30 Johnson & Johnson Clinical Diagnostics, Inc. Whole blood sample preparation for polymerase chain reaction using ammonium chloride and a carboxylic acid or metal carboxylate for selective red blood cell lysis
US5777098A (en) * 1996-07-23 1998-07-07 University Of North Dakota Medical Education Research Foundation DNA purification procedure
JP4025399B2 (ja) * 1997-10-28 2007-12-19 株式会社日立製作所 核酸の回収方法及び装置
WO1999039009A1 (en) * 1998-02-02 1999-08-05 Gentra Systems, Inc. Processes for isolating, amplifying and characterizing dna

Also Published As

Publication number Publication date
CA2319691A1 (en) 1999-08-05
AU2574299A (en) 1999-08-16
EP1053353A1 (en) 2000-11-22
JP2004500002A (ja) 2004-01-08
JP2012157366A (ja) 2012-08-23
WO1999039010A1 (en) 1999-08-05

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Effective date: 20190204