CA2319141A1 - Preparation d'un vaccin oral contre le syndrome dysgenesique respiratoire porcin dans des plantes - Google Patents

Preparation d'un vaccin oral contre le syndrome dysgenesique respiratoire porcin dans des plantes Download PDF

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Publication number
CA2319141A1
CA2319141A1 CA002319141A CA2319141A CA2319141A1 CA 2319141 A1 CA2319141 A1 CA 2319141A1 CA 002319141 A CA002319141 A CA 002319141A CA 2319141 A CA2319141 A CA 2319141A CA 2319141 A1 CA2319141 A1 CA 2319141A1
Authority
CA
Canada
Prior art keywords
plant
protein
gene
sequence
dna molecule
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002319141A
Other languages
English (en)
Inventor
Robert Rymerson
Jian Zhang
Dongwan Yoo
Larry Erickson
Jim Brandle
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Canada, AS REPRESENTED BY MINISTE TURE
University of Guelph
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CA002221843A external-priority patent/CA2221843A1/fr
Application filed by Individual filed Critical Individual
Priority to CA002319141A priority Critical patent/CA2319141A1/fr
Publication of CA2319141A1 publication Critical patent/CA2319141A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/10011—Arteriviridae
    • C12N2770/10022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La présente invention concerne un procédé de préparation d'une protéine virale du syndrome dysgénique respiratoire porcin. Ce procédé consiste tout d'abord à obtenir un gène codant la protéine désirée, à l'introduire ensuite dans un vecteur approprié pour transformer une plante, puis à transformer une plante avec ce vecteur. Après la croissance de la plante transformée, on peut récolter les tissus désirés et en extraire la protéine précitée. Cette invention concerne par ailleurs un procédé destiné à immuniser les porcs contre le syndrome dysgénésique respiratoire, consistant à récolter la plante transformée de manière à obtenir des tissus récoltés qui seront ensuite administrés aux cochons, ou à extraire ladite protéine des tissus récoltés et à l'administrer aux cochons comme supplément alimentaire, ou en injection. On peut néanmoins laisser paître les cochons directement dans les tissus végétaux avant leur récolte.
CA002319141A 1998-01-27 1999-01-27 Preparation d'un vaccin oral contre le syndrome dysgenesique respiratoire porcin dans des plantes Abandoned CA2319141A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA002319141A CA2319141A1 (fr) 1998-01-27 1999-01-27 Preparation d'un vaccin oral contre le syndrome dysgenesique respiratoire porcin dans des plantes

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
CA002221843A CA2221843A1 (fr) 1998-01-27 1998-01-27 Production, dans des plantes, de vaccins oraux contre le syndrome dysgenesique et respiratoire du porc
CA2,221,843 1998-01-27
CA002319141A CA2319141A1 (fr) 1998-01-27 1999-01-27 Preparation d'un vaccin oral contre le syndrome dysgenesique respiratoire porcin dans des plantes
PCT/CA1999/000064 WO1999037784A1 (fr) 1998-01-27 1999-01-27 Preparation d'un vaccin oral contre le syndrome dysgenesique respiratoire porcin dans des plantes

Publications (1)

Publication Number Publication Date
CA2319141A1 true CA2319141A1 (fr) 1999-07-29

Family

ID=25679857

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002319141A Abandoned CA2319141A1 (fr) 1998-01-27 1999-01-27 Preparation d'un vaccin oral contre le syndrome dysgenesique respiratoire porcin dans des plantes

Country Status (1)

Country Link
CA (1) CA2319141A1 (fr)

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Legal Events

Date Code Title Description
FZDE Discontinued