CA2312891A1 - Method and apparatus for determining the rates of reactions in liquids by mass spectrometry - Google Patents
Method and apparatus for determining the rates of reactions in liquids by mass spectrometry Download PDFInfo
- Publication number
- CA2312891A1 CA2312891A1 CA002312891A CA2312891A CA2312891A1 CA 2312891 A1 CA2312891 A1 CA 2312891A1 CA 002312891 A CA002312891 A CA 002312891A CA 2312891 A CA2312891 A CA 2312891A CA 2312891 A1 CA2312891 A1 CA 2312891A1
- Authority
- CA
- Canada
- Prior art keywords
- reactants
- source
- outlet
- reaction
- volume
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000000034 method Methods 0.000 title claims abstract description 57
- 238000006243 chemical reaction Methods 0.000 title claims abstract description 55
- 239000007788 liquid Substances 0.000 title claims description 25
- 238000004949 mass spectrometry Methods 0.000 title description 7
- 239000000376 reactant Substances 0.000 claims abstract description 51
- 150000002500 ions Chemical class 0.000 claims abstract description 48
- 238000002156 mixing Methods 0.000 claims abstract description 30
- 230000035484 reaction time Effects 0.000 claims abstract description 23
- 238000001819 mass spectrum Methods 0.000 claims abstract description 12
- 230000008859 change Effects 0.000 claims description 11
- 239000003153 chemical reaction reagent Substances 0.000 claims description 11
- 239000000543 intermediate Substances 0.000 claims description 10
- 239000000126 substance Substances 0.000 claims description 9
- 239000000047 product Substances 0.000 claims description 7
- 238000004458 analytical method Methods 0.000 claims description 3
- 238000007599 discharging Methods 0.000 claims description 3
- 230000007246 mechanism Effects 0.000 abstract description 3
- 238000000132 electrospray ionisation Methods 0.000 description 23
- 102000004169 proteins and genes Human genes 0.000 description 18
- 108090000623 proteins and genes Proteins 0.000 description 18
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 14
- 239000000243 solution Substances 0.000 description 13
- 238000001228 spectrum Methods 0.000 description 12
- 238000000065 atmospheric pressure chemical ionisation Methods 0.000 description 10
- 238000002474 experimental method Methods 0.000 description 8
- 238000009826 distribution Methods 0.000 description 7
- 238000000119 electrospray ionisation mass spectrum Methods 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 229960000583 acetic acid Drugs 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 238000002330 electrospray ionisation mass spectrometry Methods 0.000 description 5
- 238000005259 measurement Methods 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 229910001220 stainless steel Inorganic materials 0.000 description 5
- 239000010935 stainless steel Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 102100030856 Myoglobin Human genes 0.000 description 4
- 108010062374 Myoglobin Proteins 0.000 description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 4
- 238000010494 dissociation reaction Methods 0.000 description 4
- 230000005593 dissociations Effects 0.000 description 4
- 239000005350 fused silica glass Substances 0.000 description 4
- 238000005286 illumination Methods 0.000 description 4
- MMMNTDFSPSQXJP-UHFFFAOYSA-N orphenadrine citrate Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O.C=1C=CC=C(C)C=1C(OCCN(C)C)C1=CC=CC=C1 MMMNTDFSPSQXJP-UHFFFAOYSA-N 0.000 description 4
- 239000000523 sample Substances 0.000 description 4
- 239000002904 solvent Substances 0.000 description 4
- YZCKVEUIGOORGS-OUBTZVSYSA-N Deuterium Chemical group [2H] YZCKVEUIGOORGS-OUBTZVSYSA-N 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 102000008015 Hemeproteins Human genes 0.000 description 3
- 108010089792 Hemeproteins Proteins 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 239000007795 chemical reaction product Substances 0.000 description 3
- 238000004925 denaturation Methods 0.000 description 3
- 230000036425 denaturation Effects 0.000 description 3
- 229910052805 deuterium Inorganic materials 0.000 description 3
- 239000007789 gas Substances 0.000 description 3
- 150000003278 haem Chemical group 0.000 description 3
- 229910052739 hydrogen Inorganic materials 0.000 description 3
- 239000001257 hydrogen Substances 0.000 description 3
- 125000004435 hydrogen atom Chemical group [H]* 0.000 description 3
- 230000002186 photoactivation Effects 0.000 description 3
- 241000894007 species Species 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 229910001369 Brass Inorganic materials 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 239000012491 analyte Substances 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000010951 brass Substances 0.000 description 2
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- 238000005070 sampling Methods 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 238000004885 tandem mass spectrometry Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241001432959 Chernes Species 0.000 description 1
- 102100030497 Cytochrome c Human genes 0.000 description 1
- 108010075031 Cytochromes c Proteins 0.000 description 1
- 101100345589 Mus musculus Mical1 gene Proteins 0.000 description 1
- 101000744152 Naja oxiana Cytotoxin 2 Proteins 0.000 description 1
- 241000219793 Trifolium Species 0.000 description 1
- 108010062636 apomyoglobin Proteins 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000002902 bimodal effect Effects 0.000 description 1
- 239000007806 chemical reaction intermediate Substances 0.000 description 1
- 238000001360 collision-induced dissociation Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000004807 desolvation Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
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- 238000009792 diffusion process Methods 0.000 description 1
- 230000003292 diminished effect Effects 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 238000010265 fast atom bombardment Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000012362 glacial acetic acid Substances 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 238000006317 isomerization reaction Methods 0.000 description 1
- 239000006193 liquid solution Substances 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 238000011045 prefiltration Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000005476 soldering Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 230000001960 triggered effect Effects 0.000 description 1
- 108020005087 unfolded proteins Proteins 0.000 description 1
Classifications
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/02—Details
- H01J49/04—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
- H01J49/0431—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components for liquid samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/08—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
- G01N35/085—Flow Injection Analysis
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Electron Tubes For Measurement (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/986,140 US6054709A (en) | 1997-12-05 | 1997-12-05 | Method and apparatus for determining the rates of reactions in liquids by mass spectrometry |
| US08/986,140 | 1997-12-05 | ||
| PCT/CA1998/001120 WO1999030349A1 (en) | 1997-12-05 | 1998-12-03 | Method and apparatus for determining the rates of reactions in liquids by mass spectrometry |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2312891A1 true CA2312891A1 (en) | 1999-06-17 |
Family
ID=25532120
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002312891A Abandoned CA2312891A1 (en) | 1997-12-05 | 1998-12-03 | Method and apparatus for determining the rates of reactions in liquids by mass spectrometry |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US6054709A (https=) |
| EP (1) | EP1036404B1 (https=) |
| JP (1) | JP2001526398A (https=) |
| AT (1) | ATE354173T1 (https=) |
| AU (1) | AU1431099A (https=) |
| CA (1) | CA2312891A1 (https=) |
| DE (1) | DE69837106T2 (https=) |
| WO (1) | WO1999030349A1 (https=) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6350987B1 (en) * | 1998-03-31 | 2002-02-26 | Dexter B. Northrup | Enzymatic reaction mechanisms by quenched-flow mass spectrometry |
| US6630664B1 (en) * | 1999-02-09 | 2003-10-07 | Syagen Technology | Atmospheric pressure photoionizer for mass spectrometry |
| JP4527353B2 (ja) * | 2000-10-23 | 2010-08-18 | サイモン フレーザー ユニバーシティー | 個別液滴を生成する方法及び機器 |
| JP4586275B2 (ja) * | 2001-01-17 | 2010-11-24 | 味の素株式会社 | 複合体の界面を同定する方法 |
| CA2387316A1 (en) * | 2002-05-31 | 2003-11-30 | The University Of Western Ontario | Method and apparatus for the detection of noncovalent interactions by mass spectrometry - based diffusion measurements |
| US20060228696A1 (en) * | 2002-06-27 | 2006-10-12 | Robert Rohwer | Methods for detecting and inactivating a prion |
| WO2004038752A2 (en) * | 2002-10-21 | 2004-05-06 | The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Contiguous capillary electrospray sources and analytical device |
| US20090068640A1 (en) * | 2003-06-27 | 2009-03-12 | Robert Rohwer | Methods for detecting and inactivating a prion |
| JP3787578B2 (ja) * | 2003-10-29 | 2006-06-21 | アイダエンジニアリング株式会社 | マイクロチップの微細流路における液体送液方法 |
| US6870154B1 (en) | 2004-02-27 | 2005-03-22 | The University Of Western Ontario | Capillary mixer with adjustable reaction chamber volume for mass spectrometry |
| WO2011146425A1 (en) * | 2010-05-17 | 2011-11-24 | Ohio University | Microsecond time-resolved mass spectrometry |
| GB201019337D0 (en) * | 2010-11-16 | 2010-12-29 | Micromass Ltd | Controlling hydrogen-deuterium exchange on a spectrum by spectrum basis |
| CN104737025B (zh) | 2012-07-07 | 2018-02-27 | 建奥迪斯有限公司 | 用于生物化学传感器的流动管道系统 |
| CN105164783A (zh) * | 2013-04-30 | 2015-12-16 | 佳能株式会社 | 液滴喷射装置和离子源 |
| WO2015019159A1 (en) * | 2013-08-07 | 2015-02-12 | Dh Technologies Development Pte. Ltd. | Bubble removal from liquid flow into a mass spectrometer source |
| CN106683973A (zh) * | 2017-01-17 | 2017-05-17 | 中国科学技术大学 | 一种用于质谱仪的电喷雾电离源装置及系统 |
| JP7064746B2 (ja) * | 2018-02-14 | 2022-05-11 | 国立大学法人浜松医科大学 | イオン化装置、イオン化方法、プログラム、及び分析システム |
| US20220250102A1 (en) * | 2019-07-31 | 2022-08-11 | National Institute Of Advanced Industrial Science And Technology | Spray ionization device, analysis device, and surface coating device |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5009849A (en) * | 1984-12-14 | 1991-04-23 | Monsanto Company | Apparatus for carrying out catalyzed chemical reactions and for studying catalysis |
| DE3672710D1 (de) * | 1985-08-21 | 1990-08-23 | Kratos Analytical Ltd | Vorrichtung und verfahren zum gebrauch bei der massenanalyse von chemischen proben. |
| US4861988A (en) * | 1987-09-30 | 1989-08-29 | Cornell Research Foundation, Inc. | Ion spray apparatus and method |
| US4935624A (en) * | 1987-09-30 | 1990-06-19 | Cornell Research Foundation, Inc. | Thermal-assisted electrospray interface (TAESI) for LC/MS |
| US5245186A (en) * | 1991-11-18 | 1993-09-14 | The Rockefeller University | Electrospray ion source for mass spectrometry |
| US5608217A (en) * | 1994-03-10 | 1997-03-04 | Bruker-Franzen Analytik Gmbh | Electrospraying method for mass spectrometric analysis |
| DE4415480C2 (de) * | 1994-05-02 | 1999-09-02 | Bruker Daltonik Gmbh | Vorrichtung und Verfahren zur massenspektrometrischen Untersuchung von Substanzgemischen durch Kopplung kapillarelektrophoretischer Separation (CE) mit Elektrospray-Ionisierung (ESI) |
-
1997
- 1997-12-05 US US08/986,140 patent/US6054709A/en not_active Expired - Fee Related
-
1998
- 1998-12-03 AU AU14310/99A patent/AU1431099A/en not_active Abandoned
- 1998-12-03 DE DE69837106T patent/DE69837106T2/de not_active Expired - Fee Related
- 1998-12-03 WO PCT/CA1998/001120 patent/WO1999030349A1/en not_active Ceased
- 1998-12-03 JP JP2000524808A patent/JP2001526398A/ja active Pending
- 1998-12-03 AT AT98958132T patent/ATE354173T1/de not_active IP Right Cessation
- 1998-12-03 EP EP98958132A patent/EP1036404B1/en not_active Expired - Lifetime
- 1998-12-03 CA CA002312891A patent/CA2312891A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US6054709A (en) | 2000-04-25 |
| DE69837106T2 (de) | 2007-08-30 |
| AU1431099A (en) | 1999-06-28 |
| EP1036404A1 (en) | 2000-09-20 |
| ATE354173T1 (de) | 2007-03-15 |
| JP2001526398A (ja) | 2001-12-18 |
| EP1036404B1 (en) | 2007-02-14 |
| DE69837106D1 (de) | 2007-03-29 |
| WO1999030349A1 (en) | 1999-06-17 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| FZDE | Discontinued |