CA2305711A1 - Essais pour ligands a recepteurs nucleaires reposant sur le transfert d'energie de resonance en fluorescence - Google Patents
Essais pour ligands a recepteurs nucleaires reposant sur le transfert d'energie de resonance en fluorescence Download PDFInfo
- Publication number
- CA2305711A1 CA2305711A1 CA002305711A CA2305711A CA2305711A1 CA 2305711 A1 CA2305711 A1 CA 2305711A1 CA 002305711 A CA002305711 A CA 002305711A CA 2305711 A CA2305711 A CA 2305711A CA 2305711 A1 CA2305711 A1 CA 2305711A1
- Authority
- CA
- Canada
- Prior art keywords
- nuclear receptor
- binding domain
- ligand binding
- ppar
- cbp
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6875—Nucleoproteins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
- G01N2333/70567—Nuclear receptors, e.g. retinoic acid receptor [RAR], RXR, nuclear orphan receptors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Physics & Mathematics (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Peptides Or Proteins (AREA)
Abstract
L'invention concerne un procédé permettant d'identifier des agonistes et des antagonistes vis-à-vis de récepteurs nucléaires, qui consiste à mesurer le transfert d'énergie de résonance en fluorescence entre un récepteur nucléaire ou un domaine de liaison marqué par fluorescence et une protéine se liant au CRE, p300, un autre co-activateur nucléaire ou une partie de liaison qui lui appartient. Le procédé est simple, rapide, et peu onéreux. L'invention concerne également des récepteurs nucléaires et des co-activateurs de ces récepteurs marqués au moyen de réactifs fluorescents, qui sont destinés à être utilisés aux fins du procédé considéré.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US6138597P | 1997-10-07 | 1997-10-07 | |
US60/061,385 | 1997-10-07 | ||
PCT/US1998/021049 WO1999018124A1 (fr) | 1997-10-07 | 1998-10-06 | Essais pour ligands a recepteurs nucleaires reposant sur le transfert d'energie de resonance en fluorescence |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2305711A1 true CA2305711A1 (fr) | 1999-04-15 |
Family
ID=22035450
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002305711A Abandoned CA2305711A1 (fr) | 1997-10-07 | 1998-10-06 | Essais pour ligands a recepteurs nucleaires reposant sur le transfert d'energie de resonance en fluorescence |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP1021462A4 (fr) |
JP (1) | JP2001519525A (fr) |
CA (1) | CA2305711A1 (fr) |
WO (1) | WO1999018124A1 (fr) |
Families Citing this family (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2002528721A (ja) * | 1998-10-23 | 2002-09-03 | グラクソ グループ リミテッド | 核内受容体のリガンド用のアッセイ |
ES2326850T3 (es) | 1998-12-23 | 2009-10-20 | Glaxo Group Limited | Ensayos para ligandos de receptores nucleares. |
US6500629B1 (en) | 1999-09-13 | 2002-12-31 | Equitech Laboratories, Inc. | Materials and methods for detection and quantitation of an analyte |
WO2001040805A1 (fr) * | 1999-11-30 | 2001-06-07 | Akzo Nobel N.V. | Composes steroidiques destines a des dosages par liaison de recepteur de steroides |
AU2001250898A1 (en) * | 2000-03-17 | 2001-10-03 | The Salk Institute For Biological Studies | Compositions associated with complex formation |
US7198924B2 (en) | 2000-12-11 | 2007-04-03 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
US6924311B2 (en) | 2001-10-17 | 2005-08-02 | X-Ceptor Therapeutics, Inc. | Methods for affecting various diseases utilizing LXR compounds |
ATE313803T1 (de) | 2001-11-07 | 2006-01-15 | Schering Ag | In vitro screening nach liganden des östrogenrezeptors |
KR20050026488A (ko) * | 2002-07-24 | 2005-03-15 | 3-디멘져널 파마슈티칼즈 인코오포레이티드 | 리간드의 동정 방법 |
ES2306900T3 (es) | 2002-11-07 | 2008-11-16 | Erasmus Universiteit Rotterdam | Sondas fret y metodos para detectar moleculas de interaccion. |
JP4803976B2 (ja) * | 2003-07-09 | 2011-10-26 | 独立行政法人科学技術振興機構 | 細胞内ip3測定用分子センサー |
AU2004268647B2 (en) | 2003-08-28 | 2010-07-01 | Choongwae Pharma Corporation | Modulation of beta-catenin/TCF activated transcription |
WO2005054438A2 (fr) | 2003-12-01 | 2005-06-16 | Invitrogen Corporation | Molecule d'acide nucleique contenant des sites de recombinaison et leurs procedes d'utilisation |
EP1733237A2 (fr) * | 2004-03-26 | 2006-12-20 | Bayer HealthCare AG | Diagnostic et traitement de maladies associees au recepteur active de la proliferation des peroxysomes alpha (ppara) |
KR100704011B1 (ko) | 2005-02-16 | 2007-04-04 | 한국과학기술원 | 금속나노입자와 양자점의 fret에 의한 생체분자특이결합 검출 방법 |
JP4936476B2 (ja) * | 2005-06-28 | 2012-05-23 | 第一三共株式会社 | Lxrリガンドの試験方法 |
JP5264421B2 (ja) | 2007-11-22 | 2013-08-14 | 富士フイルム株式会社 | 被験物測定用担体およびその製造方法 |
WO2009141926A1 (fr) * | 2008-05-23 | 2009-11-26 | 国立大学法人東京大学 | Procédé d'acquisition d'un composé capable d'agir sur le métabolisme du glucose/métabolisme lipidique |
WO2015159293A2 (fr) * | 2014-04-14 | 2015-10-22 | Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. | Procédé et kit permettant de déterminer l'origine tissulaire ou cellulaire de l'adn |
JP2019521673A (ja) * | 2016-06-07 | 2019-08-08 | ザ リージェンツ オブ ザ ユニヴァーシティー オブ カリフォルニア | 疾患および状態の分析のためのセルフリーdnaメチル化パターン |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB9608143D0 (en) * | 1996-04-19 | 1996-06-26 | Ver Nl Kanker Inst | Assay |
WO1998056806A1 (fr) * | 1997-06-12 | 1998-12-17 | The Regents Of The University Of California | Proteine de coactivation de facteur de transcription, p/cip |
AU2533499A (en) * | 1998-02-12 | 1999-08-30 | Prolifix Limited | Interaction between cyclin d1 and steroid receptor co-activators and uses thereof in assays |
-
1998
- 1998-10-06 EP EP98956094A patent/EP1021462A4/fr not_active Withdrawn
- 1998-10-06 CA CA002305711A patent/CA2305711A1/fr not_active Abandoned
- 1998-10-06 JP JP2000514931A patent/JP2001519525A/ja active Pending
- 1998-10-06 WO PCT/US1998/021049 patent/WO1999018124A1/fr active Application Filing
Also Published As
Publication number | Publication date |
---|---|
EP1021462A4 (fr) | 2005-04-13 |
JP2001519525A (ja) | 2001-10-23 |
WO1999018124A1 (fr) | 1999-04-15 |
EP1021462A1 (fr) | 2000-07-26 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
FZDE | Discontinued |