CA2305711A1 - Essais pour ligands a recepteurs nucleaires reposant sur le transfert d'energie de resonance en fluorescence - Google Patents

Essais pour ligands a recepteurs nucleaires reposant sur le transfert d'energie de resonance en fluorescence Download PDF

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Publication number
CA2305711A1
CA2305711A1 CA002305711A CA2305711A CA2305711A1 CA 2305711 A1 CA2305711 A1 CA 2305711A1 CA 002305711 A CA002305711 A CA 002305711A CA 2305711 A CA2305711 A CA 2305711A CA 2305711 A1 CA2305711 A1 CA 2305711A1
Authority
CA
Canada
Prior art keywords
nuclear receptor
binding domain
ligand binding
ppar
cbp
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002305711A
Other languages
English (en)
Inventor
Richard T. Cummings
Jeffrey D. Hermes
David E. Moller
Gaochao Zhou
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck and Co Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2305711A1 publication Critical patent/CA2305711A1/fr
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6875—Nucleoproteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/70567—Nuclear receptors, e.g. retinoic acid receptor [RAR], RXR, nuclear orphan receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)

Abstract

L'invention concerne un procédé permettant d'identifier des agonistes et des antagonistes vis-à-vis de récepteurs nucléaires, qui consiste à mesurer le transfert d'énergie de résonance en fluorescence entre un récepteur nucléaire ou un domaine de liaison marqué par fluorescence et une protéine se liant au CRE, p300, un autre co-activateur nucléaire ou une partie de liaison qui lui appartient. Le procédé est simple, rapide, et peu onéreux. L'invention concerne également des récepteurs nucléaires et des co-activateurs de ces récepteurs marqués au moyen de réactifs fluorescents, qui sont destinés à être utilisés aux fins du procédé considéré.
CA002305711A 1997-10-07 1998-10-06 Essais pour ligands a recepteurs nucleaires reposant sur le transfert d'energie de resonance en fluorescence Abandoned CA2305711A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US6138597P 1997-10-07 1997-10-07
US60/061,385 1997-10-07
PCT/US1998/021049 WO1999018124A1 (fr) 1997-10-07 1998-10-06 Essais pour ligands a recepteurs nucleaires reposant sur le transfert d'energie de resonance en fluorescence

Publications (1)

Publication Number Publication Date
CA2305711A1 true CA2305711A1 (fr) 1999-04-15

Family

ID=22035450

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002305711A Abandoned CA2305711A1 (fr) 1997-10-07 1998-10-06 Essais pour ligands a recepteurs nucleaires reposant sur le transfert d'energie de resonance en fluorescence

Country Status (4)

Country Link
EP (1) EP1021462A4 (fr)
JP (1) JP2001519525A (fr)
CA (1) CA2305711A1 (fr)
WO (1) WO1999018124A1 (fr)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000025134A1 (fr) * 1998-10-23 2000-05-04 Glaxo Group Limited Procede d'identification de ligands de recepteurs nucleaires
JP2002532729A (ja) 1998-12-23 2002-10-02 グラクソ グループ リミテッド 核内受容体のリガンドのアッセイ
WO2001020326A2 (fr) * 1999-09-13 2001-03-22 Equitech Laboratories, Inc. Materiaux et procede de detection et de quantification d'un analysat
EP1238285A1 (fr) * 1999-11-30 2002-09-11 Akzo Nobel N.V. Composes steroidiques destines a des dosages par liaison de recepteur de steroides
AU2001250898A1 (en) * 2000-03-17 2001-10-03 The Salk Institute For Biological Studies Compositions associated with complex formation
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US6924311B2 (en) 2001-10-17 2005-08-02 X-Ceptor Therapeutics, Inc. Methods for affecting various diseases utilizing LXR compounds
ATE313803T1 (de) 2001-11-07 2006-01-15 Schering Ag In vitro screening nach liganden des östrogenrezeptors
JP2005534011A (ja) * 2002-07-24 2005-11-10 ３−ディメンショナル ファーマシューティカルズ， インコーポレイテッド 生体異物除去の調節を決定する方法
CA2505515C (fr) * 2002-11-07 2013-08-27 Erasmus Universiteit Rotterdam Sondes fret et procedes de detection de molecules d'interaction
JP4803976B2 (ja) * 2003-07-09 2011-10-26 独立行政法人科学技術振興機構 細胞内ｉｐ３測定用分子センサー
NZ545568A (en) * 2003-08-28 2009-06-26 Choongwae Pharma Corp Use of compounds that modulate beta-catenin/TCF activated transcription for manufacture of a medicamnet to treat cancer
EP1697534B1 (fr) 2003-12-01 2010-06-02 Life Technologies Corporation Molecule d'acide nucleique contenant des sites de recombinaison et leurs procedes d'utilisation
US20080286260A1 (en) * 2004-03-26 2008-11-20 Bayer Healthcare Ag Diagnostic and Therapeutics for Diseases Associated with Peroxisome Proliferative Activated Receptor Alpha (Ppara)
KR100704011B1 (ko) 2005-02-16 2007-04-04 한국과학기술원 금속나노입자와 양자점의 ｆｒｅｔ에 의한 생체분자특이결합 검출 방법
US7989179B2 (en) 2005-06-28 2011-08-02 Daiichi Sankyo Company, Limited LXR ligand testing method
JP5264421B2 (ja) 2007-11-22 2013-08-14 富士フイルム株式会社 被験物測定用担体およびその製造方法
WO2009141926A1 (fr) * 2008-05-23 2009-11-26 国立大学法人東京大学 Procédé d'acquisition d'un composé capable d'agir sur le métabolisme du glucose/métabolisme lipidique
PT4026917T (pt) 2014-04-14 2024-02-12 Yissum Research And Development Company Of The Hebrew Univ Of Jerusalem Ltd Método e kit para determinar a morte de células ou de tecido ou a origem de tecidos ou de células de dna por análise de metilação do dna
CN110168099B (zh) 2016-06-07 2024-06-07 加利福尼亚大学董事会 用于疾病和病症分析的无细胞dna甲基化模式

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9608143D0 (en) * 1996-04-19 1996-06-26 Ver Nl Kanker Inst Assay
AU8142198A (en) * 1997-06-12 1998-12-30 Regents Of The University Of California, The A transcription factor coactivator protein, p/cip
JP2002503811A (ja) * 1998-02-12 2002-02-05 プロリフィクス リミテッド サイクリンｄ１とステロイド受容体コアクチベーターとの相互作用、およびアッセイにおけるその使用

Also Published As

Publication number Publication date
EP1021462A4 (fr) 2005-04-13
WO1999018124A1 (fr) 1999-04-15
EP1021462A1 (fr) 2000-07-26
JP2001519525A (ja) 2001-10-23

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued