CA2302201A1 - Procede et ensemble de materiel d'evaluation de mutations vih - Google Patents

Procede et ensemble de materiel d'evaluation de mutations vih Download PDF

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Publication number
CA2302201A1
CA2302201A1 CA002302201A CA2302201A CA2302201A1 CA 2302201 A1 CA2302201 A1 CA 2302201A1 CA 002302201 A CA002302201 A CA 002302201A CA 2302201 A CA2302201 A CA 2302201A CA 2302201 A1 CA2302201 A1 CA 2302201A1
Authority
CA
Canada
Prior art keywords
seq
hiv
primer
sequencing
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002302201A
Other languages
English (en)
Inventor
James M. Dunn
Jean-Michel Lacroix
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer Healthcare LLC
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/938,641 external-priority patent/US6007983A/en
Application filed by Individual filed Critical Individual
Publication of CA2302201A1 publication Critical patent/CA2302201A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Virology (AREA)
  • AIDS & HIV (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé rationalisé et hiérarchique permettant d'obtenir des informations sur le type allélique d'un échantillon de matériel génétique dérivé d'un échantillon infecté par VIH. On peut déterminer entre 93 et 95 % des variants mutationnels connus des gènes de transcriptase inverse et de protéase de VIH-1 en évaluant les positions des nucléotides A et T à l'intérieur de l'échantillon. Ainsi, on peut identifier de manière univoque une portion considérable de toutes les variations mutationnelles en effectuant deux réactions de séquençage initiales sur l'échantillon dans lequel seuls les ddA et ddT sont utilisés comme agents de terminaison de chaîne. Pour la petite proportion d'échantillons qui ne sont pas identifiables en fonction des positions de ces deux bases, on effectue une seconde épreuve au cours de laquelle on détermine la séquence dans la direction 3' pour les quatre bases. Cette épreuve permet d'identifier sensiblement tous les échantillons restants. Pour ceux pour lesquels une ambiguïté subsiste, on effectue une épreuve finale dans laquelle la séquence de l'échantillon est déterminée dans les directions 3' et 5' pour les quatre bases. Pour mettre en oeuvre ce procédé, les réactifs convenant à la réalisation de ces trois épreuves dans l'ordre hiérarchique sont convenablement emballés sous forme d'ensemble de matériel contenant deux ou plusieurs sous-ensembles. Le premier sous-ensemble renferme des réactifs destinés à effectuer le séquençage A et T. Les sous-ensembles supplémentaires renferment des réactifs destinés à effectuer une détermination d'une séquence à quatre bases sur un ou plusieurs brins de l'ADN cible.
CA002302201A 1997-09-26 1998-09-28 Procede et ensemble de materiel d'evaluation de mutations vih Abandoned CA2302201A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/938,641 US6007983A (en) 1995-12-22 1997-09-26 Method and kit for evaluation of HIV mutations
US08/938,641 1997-09-26
PCT/CA1998/000913 WO1999016910A1 (fr) 1997-09-26 1998-09-28 Procede et ensemble de materiel d'evaluation de mutations vih

Publications (1)

Publication Number Publication Date
CA2302201A1 true CA2302201A1 (fr) 1999-04-08

Family

ID=25471725

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002302201A Abandoned CA2302201A1 (fr) 1997-09-26 1998-09-28 Procede et ensemble de materiel d'evaluation de mutations vih

Country Status (5)

Country Link
EP (1) EP1017856A1 (fr)
JP (1) JP2001518313A (fr)
AU (1) AU751471B2 (fr)
CA (1) CA2302201A1 (fr)
WO (1) WO1999016910A1 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6265152B1 (en) * 1995-12-22 2001-07-24 Visible Genetics Inc. Method and kit for evaluation of HIV mutations
US6623920B1 (en) 1999-07-09 2003-09-23 Gen-Probe Incorporated Detection of HIV-1 by nucleic acid amplification
GB2395009B (en) * 1999-10-15 2004-07-21 Visible Genetics Inc Method and kit for evaluation of HIV mutations
GB2395787A (en) * 1999-10-15 2004-06-02 Visible Genetics Inc Method and kit for evaluation of HIV mutations
US6582920B2 (en) 2000-09-01 2003-06-24 Gen-Probe Incorporated Amplification of HIV-1 RT sequences for detection of sequences associated with drug-resistance mutations
CA2835978C (fr) * 2000-09-01 2016-10-11 Gen-Probe Incorporated Amplification de sequences vih-1 pour la detection de sequences associees aux mutations de la resistance aux medicaments
ES2610781T3 (es) * 2004-09-14 2017-05-03 Argos Therapeutics, Inc. Amplificación de patógenos independiente de la cepa y vacunas para estos

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5629153A (en) * 1990-01-10 1997-05-13 Chiron Corporation Use of DNA-dependent RNA polymerase transcripts as reporter molecules for signal amplification in nucleic acid hybridization assays
JP3907201B2 (ja) * 1991-12-23 2007-04-18 カイロン コーポレイション 溶液相サンドイッチハイブリダイゼーションアッセイに用いるためのｈｉｖプローブ
US5834189A (en) * 1994-07-08 1998-11-10 Visible Genetics Inc. Method for evaluation of polymorphic genetic sequences, and the use thereof in identification of HLA types
US6013436A (en) * 1994-07-08 2000-01-11 Visible Genetics, Inc. Compositions and methods for diagnosis of mutation in the von Hippel-Lindau tumor suppressor gene
US6071726A (en) * 1994-07-08 2000-06-06 Visible Genetics Inc. Method, reagents and kit for diagnosis and targeted screening for p53 mutations
US5795722A (en) * 1997-03-18 1998-08-18 Visible Genetics Inc. Method and kit for quantitation and nucleic acid sequencing of nucleic acid analytes in a sample

Also Published As

Publication number Publication date
AU9249398A (en) 1999-04-23
WO1999016910A1 (fr) 1999-04-08
AU751471B2 (en) 2002-08-15
EP1017856A1 (fr) 2000-07-12
JP2001518313A (ja) 2001-10-16

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued