CA2299388A1 - Processes for increasing the yield in plants - Google Patents
Processes for increasing the yield in plants Download PDFInfo
- Publication number
- CA2299388A1 CA2299388A1 CA002299388A CA2299388A CA2299388A1 CA 2299388 A1 CA2299388 A1 CA 2299388A1 CA 002299388 A CA002299388 A CA 002299388A CA 2299388 A CA2299388 A CA 2299388A CA 2299388 A1 CA2299388 A1 CA 2299388A1
- Authority
- CA
- Canada
- Prior art keywords
- plants
- plant
- sucrose
- nucleotide sequence
- cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000000034 method Methods 0.000 title claims abstract description 30
- 230000008569 process Effects 0.000 title claims abstract description 24
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 43
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 24
- 230000000638 stimulation Effects 0.000 claims abstract description 7
- 241000196324 Embryophyta Species 0.000 claims description 152
- 210000004027 cell Anatomy 0.000 claims description 59
- 244000061456 Solanum tuberosum Species 0.000 claims description 36
- 239000013598 vector Substances 0.000 claims description 29
- 229930006000 Sucrose Natural products 0.000 claims description 24
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 24
- 239000005720 sucrose Substances 0.000 claims description 24
- 239000002773 nucleotide Substances 0.000 claims description 23
- 125000003729 nucleotide group Chemical group 0.000 claims description 23
- 235000018102 proteins Nutrition 0.000 claims description 23
- 230000009261 transgenic effect Effects 0.000 claims description 23
- 108020004414 DNA Proteins 0.000 claims description 19
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 17
- 230000009466 transformation Effects 0.000 claims description 15
- 108020004707 nucleic acids Proteins 0.000 claims description 14
- 102000039446 nucleic acids Human genes 0.000 claims description 14
- 150000007523 nucleic acids Chemical class 0.000 claims description 14
- 108010043934 Sucrose synthase Proteins 0.000 claims description 13
- 102000006732 Citrate synthase Human genes 0.000 claims description 12
- 230000000694 effects Effects 0.000 claims description 12
- 108091006112 ATPases Proteins 0.000 claims description 11
- 102000057290 Adenosine Triphosphatases Human genes 0.000 claims description 11
- 241000589156 Agrobacterium rhizogenes Species 0.000 claims description 9
- 241000233866 Fungi Species 0.000 claims description 8
- 101150013395 ROLC gene Proteins 0.000 claims description 8
- 238000013518 transcription Methods 0.000 claims description 8
- 230000035897 transcription Effects 0.000 claims description 8
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 7
- 235000009337 Spinacia oleracea Nutrition 0.000 claims description 7
- 244000300264 Spinacia oleracea Species 0.000 claims description 7
- 210000000170 cell membrane Anatomy 0.000 claims description 7
- 108700038781 Citrate synthases Proteins 0.000 claims description 6
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 6
- 108010051210 beta-Fructofuranosidase Proteins 0.000 claims description 6
- 230000010354 integration Effects 0.000 claims description 6
- 235000011073 invertase Nutrition 0.000 claims description 6
- 229920001184 polypeptide Polymers 0.000 claims description 6
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 6
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 6
- 108020000005 Sucrose phosphorylase Proteins 0.000 claims description 5
- 230000002255 enzymatic effect Effects 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 5
- 241000894006 Bacteria Species 0.000 claims description 3
- 108010064851 Plant Proteins Proteins 0.000 claims description 2
- 108020004511 Recombinant DNA Proteins 0.000 claims description 2
- 235000021118 plant-derived protein Nutrition 0.000 claims description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract description 4
- 239000012634 fragment Substances 0.000 description 58
- 239000013612 plasmid Substances 0.000 description 33
- 238000003776 cleavage reaction Methods 0.000 description 22
- 230000007017 scission Effects 0.000 description 22
- 238000003752 polymerase chain reaction Methods 0.000 description 17
- 238000004519 manufacturing process Methods 0.000 description 16
- 108010078791 Carrier Proteins Proteins 0.000 description 9
- 239000002299 complementary DNA Substances 0.000 description 9
- 239000002028 Biomass Substances 0.000 description 8
- 238000004458 analytical method Methods 0.000 description 8
- 238000010367 cloning Methods 0.000 description 8
- 108091026890 Coding region Proteins 0.000 description 7
- 238000012546 transfer Methods 0.000 description 7
- 108010030844 2-methylcitrate synthase Proteins 0.000 description 6
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 6
- 108010071536 Citrate (Si)-synthase Proteins 0.000 description 6
- 229920002472 Starch Polymers 0.000 description 6
- 240000008042 Zea mays Species 0.000 description 6
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 6
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 6
- 238000010276 construction Methods 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
- 235000009973 maize Nutrition 0.000 description 6
- 230000001404 mediated effect Effects 0.000 description 6
- 239000008107 starch Substances 0.000 description 6
- 235000019698 starch Nutrition 0.000 description 6
- 241000589158 Agrobacterium Species 0.000 description 5
- 230000029087 digestion Effects 0.000 description 5
- 239000001573 invertase Substances 0.000 description 5
- 210000001938 protoplast Anatomy 0.000 description 5
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Natural products CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 4
- 101150114843 Mgll gene Proteins 0.000 description 4
- 238000003780 insertion Methods 0.000 description 4
- 230000037431 insertion Effects 0.000 description 4
- 239000003550 marker Substances 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 3
- 240000005979 Hordeum vulgare Species 0.000 description 3
- 235000007340 Hordeum vulgare Nutrition 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 108091034117 Oligonucleotide Proteins 0.000 description 3
- 241000235070 Saccharomyces Species 0.000 description 3
- 235000021307 Triticum Nutrition 0.000 description 3
- 244000098338 Triticum aestivum Species 0.000 description 3
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 150000007513 acids Chemical class 0.000 description 3
- 235000013339 cereals Nutrition 0.000 description 3
- 230000001086 cytosolic effect Effects 0.000 description 3
- 235000013399 edible fruits Nutrition 0.000 description 3
- 238000004520 electroporation Methods 0.000 description 3
- 229930027917 kanamycin Natural products 0.000 description 3
- 229960000318 kanamycin Drugs 0.000 description 3
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 3
- 229930182823 kanamycin A Natural products 0.000 description 3
- 239000006870 ms-medium Substances 0.000 description 3
- 210000000056 organ Anatomy 0.000 description 3
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 2
- 241000219195 Arabidopsis thaliana Species 0.000 description 2
- 235000007319 Avena orientalis Nutrition 0.000 description 2
- 244000075850 Avena orientalis Species 0.000 description 2
- 241000219310 Beta vulgaris subsp. vulgaris Species 0.000 description 2
- 101000583086 Bunodosoma granuliferum Delta-actitoxin-Bgr2b Proteins 0.000 description 2
- 108020004705 Codon Proteins 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 244000068988 Glycine max Species 0.000 description 2
- 235000010469 Glycine max Nutrition 0.000 description 2
- 206010020649 Hyperkeratosis Diseases 0.000 description 2
- 241000221961 Neurospora crassa Species 0.000 description 2
- 244000061176 Nicotiana tabacum Species 0.000 description 2
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 2
- 235000007164 Oryza sativa Nutrition 0.000 description 2
- 241000235347 Schizosaccharomyces pombe Species 0.000 description 2
- 241000209056 Secale Species 0.000 description 2
- 235000007238 Secale cereale Nutrition 0.000 description 2
- 244000062793 Sorghum vulgare Species 0.000 description 2
- 235000021536 Sugar beet Nutrition 0.000 description 2
- 235000019714 Triticale Nutrition 0.000 description 2
- ZSLZBFCDCINBPY-ZSJPKINUSA-N acetyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 ZSLZBFCDCINBPY-ZSJPKINUSA-N 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- GPRBEKHLDVQUJE-VINNURBNSA-N cefotaxime Chemical compound N([C@@H]1C(N2C(=C(COC(C)=O)CS[C@@H]21)C(O)=O)=O)C(=O)/C(=N/OC)C1=CSC(N)=N1 GPRBEKHLDVQUJE-VINNURBNSA-N 0.000 description 2
- 229940088530 claforan Drugs 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 150000002402 hexoses Chemical class 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 235000019713 millet Nutrition 0.000 description 2
- -1 naphtyl acetic acid Chemical compound 0.000 description 2
- 230000029553 photosynthesis Effects 0.000 description 2
- 238000010672 photosynthesis Methods 0.000 description 2
- 230000000243 photosynthetic effect Effects 0.000 description 2
- 230000008488 polyadenylation Effects 0.000 description 2
- 235000009566 rice Nutrition 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 244000045561 useful plants Species 0.000 description 2
- 239000002023 wood Substances 0.000 description 2
- 241000228158 x Triticosecale Species 0.000 description 2
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
- 241001541756 Acinetobacter calcoaceticus subsp. anitratus Species 0.000 description 1
- 101100108891 Arabidopsis thaliana PRMT11 gene Proteins 0.000 description 1
- 102100023927 Asparagine synthetase [glutamine-hydrolyzing] Human genes 0.000 description 1
- 108010070255 Aspartate-ammonia ligase Proteins 0.000 description 1
- 241000228212 Aspergillus Species 0.000 description 1
- 241000228197 Aspergillus flavus Species 0.000 description 1
- 241000228245 Aspergillus niger Species 0.000 description 1
- 235000007558 Avena sp Nutrition 0.000 description 1
- 235000000832 Ayote Nutrition 0.000 description 1
- 241000193749 Bacillus coagulans Species 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 108010077805 Bacterial Proteins Proteins 0.000 description 1
- 240000007124 Brassica oleracea Species 0.000 description 1
- 108020004635 Complementary DNA Proteins 0.000 description 1
- 241000219112 Cucumis Species 0.000 description 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- 240000008067 Cucumis sativus Species 0.000 description 1
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
- 235000009854 Cucurbita moschata Nutrition 0.000 description 1
- 240000001980 Cucurbita pepo Species 0.000 description 1
- 235000009804 Cucurbita pepo subsp pepo Nutrition 0.000 description 1
- 241000219130 Cucurbita pepo subsp. pepo Species 0.000 description 1
- 235000003954 Cucurbita pepo var melopepo Nutrition 0.000 description 1
- HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 1
- 102000012195 Fructose-1,6-bisphosphatases Human genes 0.000 description 1
- 108010017464 Fructose-Bisphosphatase Proteins 0.000 description 1
- 241000219146 Gossypium Species 0.000 description 1
- 241000204933 Haloferax volcanii Species 0.000 description 1
- 244000020551 Helianthus annuus Species 0.000 description 1
- 235000003222 Helianthus annuus Nutrition 0.000 description 1
- 235000008694 Humulus lupulus Nutrition 0.000 description 1
- 235000002678 Ipomoea batatas Nutrition 0.000 description 1
- 244000017020 Ipomoea batatas Species 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 244000070406 Malus silvestris Species 0.000 description 1
- 240000003183 Manihot esculenta Species 0.000 description 1
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 description 1
- 241000187480 Mycobacterium smegmatis Species 0.000 description 1
- 241000221960 Neurospora Species 0.000 description 1
- 101100484946 Petunia hybrida VPY gene Proteins 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 240000004713 Pisum sativum Species 0.000 description 1
- 235000010582 Pisum sativum Nutrition 0.000 description 1
- 241000220324 Pyrus Species 0.000 description 1
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 1
- 241000606697 Rickettsia prowazekii Species 0.000 description 1
- 108091006631 SLC13A4 Proteins 0.000 description 1
- 240000000111 Saccharum officinarum Species 0.000 description 1
- 235000007201 Saccharum officinarum Nutrition 0.000 description 1
- 241000235346 Schizosaccharomyces Species 0.000 description 1
- 241000208292 Solanaceae Species 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 235000002597 Solanum melongena Nutrition 0.000 description 1
- 244000061458 Solanum melongena Species 0.000 description 1
- 102100035209 Solute carrier family 13 member 4 Human genes 0.000 description 1
- 241000041231 Thermanaeromonas burensis Species 0.000 description 1
- 241000204673 Thermoplasma acidophilum Species 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
- 239000002390 adhesive tape Substances 0.000 description 1
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 229940024606 amino acid Drugs 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 230000000692 anti-sense effect Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 235000021016 apples Nutrition 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229940049580 biozide Drugs 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 230000002074 deregulated effect Effects 0.000 description 1
- 210000000416 exudates and transudate Anatomy 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- JLJLRLWOEMWYQK-GDUNQVSHSA-N giberellic acid Chemical compound C([C@@]1(O)C(=C)C[C@@]2(C1)C1C(O)=O)CC2[C@@]2(OC3=O)C1[C@]3(C)[C@@H](O)CC2 JLJLRLWOEMWYQK-GDUNQVSHSA-N 0.000 description 1
- 229930002203 giberellic acid Natural products 0.000 description 1
- 239000003365 glass fiber Substances 0.000 description 1
- 238000003898 horticulture Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000004807 localization Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- WYXYBEOWQBGFQB-UHFFFAOYSA-N n-benzyl-7h-purin-2-amine Chemical compound N=1C=C2NC=NC2=NC=1NCC1=CC=CC=C1 WYXYBEOWQBGFQB-UHFFFAOYSA-N 0.000 description 1
- 101150091418 pam1 gene Proteins 0.000 description 1
- 235000021017 pears Nutrition 0.000 description 1
- 239000013600 plasmid vector Substances 0.000 description 1
- 235000015136 pumpkin Nutrition 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- UZKQTCBAMSWPJD-UQCOIBPSSA-N trans-Zeatin Natural products OCC(/C)=C\CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-UQCOIBPSSA-N 0.000 description 1
- UZKQTCBAMSWPJD-FARCUNLSSA-N trans-zeatin Chemical compound OCC(/C)=C/CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-FARCUNLSSA-N 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 229940023877 zeatin Drugs 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
- C12N9/1062—Sucrose synthase (2.4.1.13)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
- C12N15/8223—Vegetative tissue-specific promoters
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2431—Beta-fructofuranosidase (3.2.1.26), i.e. invertase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01026—Beta-fructofuranosidase (3.2.1.26), i.e. invertase
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Nutrition Science (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19734218.3 | 1997-08-07 | ||
| DE19734218A DE19734218A1 (de) | 1997-08-07 | 1997-08-07 | Verfahren zur Ertragssteigerung in Pflanzen |
| PCT/EP1998/004878 WO1999007863A1 (en) | 1997-08-07 | 1998-08-05 | Processes for increasing the yield in plants |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2299388A1 true CA2299388A1 (en) | 1999-02-18 |
Family
ID=7838278
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002299388A Abandoned CA2299388A1 (en) | 1997-08-07 | 1998-08-05 | Processes for increasing the yield in plants |
Country Status (11)
| Country | Link |
|---|---|
| EP (1) | EP1002118A1 (hu) |
| JP (1) | JP2001512685A (hu) |
| KR (1) | KR20010021566A (hu) |
| CN (1) | CN1265704A (hu) |
| AU (1) | AU734951B2 (hu) |
| BR (1) | BR9811853A (hu) |
| CA (1) | CA2299388A1 (hu) |
| DE (1) | DE19734218A1 (hu) |
| HU (1) | HUP0004258A3 (hu) |
| PL (1) | PL338561A1 (hu) |
| WO (1) | WO1999007863A1 (hu) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000023597A2 (en) * | 1998-10-16 | 2000-04-27 | Scottish Crop Research Institute | Tissue-specific promoters for gene expression |
| CA2438388A1 (en) * | 2001-02-22 | 2002-09-06 | Pioneer Hi-Bred International, Inc. | Manipulation of sucrose synthase genes to improve stalk and grain quality |
| CN101851629B (zh) * | 2010-05-24 | 2013-10-09 | 中国科学院遗传与发育生物学研究所 | 转化水稻蔗糖转运蛋白基因OsSUT5Z提高作物产量的方法和用途 |
| EP2431471A1 (en) | 2010-09-17 | 2012-03-21 | Université Catholique De Louvain | Methods for increasing the size of plants or plant organs |
| CN103880935B (zh) * | 2012-12-19 | 2017-02-08 | 中国科学院植物研究所 | 蔗糖转运蛋白AtSUT2在培育高产转基因植物中的应用 |
| AU2014370933A1 (en) * | 2013-12-27 | 2016-07-07 | National Agriculture And Food Research Organization | Transgenic plant and method for producing sugar-containing exudate that uses transgenic plant |
| DE112014006075B9 (de) * | 2013-12-27 | 2022-12-08 | National Agriculture And Food Research Organization | Transformierte Pflanze oder transformierte Pflanzenzelle und Verfahren zur Herstellung eines Exsudats |
| CN105543260B (zh) * | 2016-02-06 | 2019-03-19 | 中国热带农业科学院橡胶研究所 | HbCS4基因在提高原核表达菌生长速率、研究橡胶树产胶能力中的应用 |
| CN105671076B (zh) * | 2016-03-31 | 2019-07-12 | 西南大学 | 一种植物表达载体及其在提高棉花产量中的应用 |
| CN106834343B (zh) * | 2017-02-21 | 2019-09-13 | 中国农业大学 | 蔗糖合成酶在调控植物果实发育中的应用 |
| CN110818785B (zh) * | 2019-11-26 | 2022-04-19 | 吉林省农业科学院 | 一种玉米蔗糖转运蛋白ZmSUT3J及其编码基因和应用 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5750869A (en) * | 1995-01-15 | 1998-05-12 | Calgene, Inc. | Soluble solids modification using sucrose phosphate synthase encoding sequences |
-
1997
- 1997-08-07 DE DE19734218A patent/DE19734218A1/de not_active Withdrawn
-
1998
- 1998-08-05 BR BR9811853-6A patent/BR9811853A/pt not_active IP Right Cessation
- 1998-08-05 CA CA002299388A patent/CA2299388A1/en not_active Abandoned
- 1998-08-05 HU HU0004258A patent/HUP0004258A3/hu unknown
- 1998-08-05 KR KR1020007000124A patent/KR20010021566A/ko not_active Application Discontinuation
- 1998-08-05 AU AU92572/98A patent/AU734951B2/en not_active Ceased
- 1998-08-05 JP JP2000506346A patent/JP2001512685A/ja active Pending
- 1998-08-05 EP EP98945149A patent/EP1002118A1/en not_active Withdrawn
- 1998-08-05 CN CN98807898A patent/CN1265704A/zh active Pending
- 1998-08-05 PL PL98338561A patent/PL338561A1/xx unknown
- 1998-08-05 WO PCT/EP1998/004878 patent/WO1999007863A1/en not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| HUP0004258A2 (hu) | 2001-04-28 |
| EP1002118A1 (en) | 2000-05-24 |
| AU734951B2 (en) | 2001-06-28 |
| BR9811853A (pt) | 2000-08-08 |
| JP2001512685A (ja) | 2001-08-28 |
| DE19734218A1 (de) | 1999-02-11 |
| AU9257298A (en) | 1999-03-01 |
| PL338561A1 (en) | 2000-11-06 |
| CN1265704A (zh) | 2000-09-06 |
| WO1999007863A1 (en) | 1999-02-18 |
| HUP0004258A3 (en) | 2003-06-30 |
| KR20010021566A (ko) | 2001-03-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US5436394A (en) | Plasmid for the preparation of transgenic plants with a change in habit and yield | |
| CA2275885C (en) | Transgenic potatoes having reduced levels of alpha glucan l- or h-type tuber phosphorylase activity with reduced cold-sweetening | |
| US8269065B2 (en) | Nucleic acids and proteins associated with sucrose degradation in coffee | |
| EP0647273B1 (en) | Dna sequences encoding oligosaccharide transporter | |
| US5658773A (en) | Tomato acid invertase gene | |
| US6245967B1 (en) | Process and DNA molecules for increasing the photosynthesis rate in plants | |
| AU734951B2 (en) | Processes for increasing the yield in plants | |
| US20040197915A1 (en) | C3 plants expressing photosynthetic enzyme of C4 plants | |
| TWI534265B (zh) | 用於操縱植物之光合細胞中果聚糖生合成之方法、基因建構體及基因轉殖植物細胞 | |
| JP3538428B2 (ja) | 植物プロモーターおよび該プロモーターを用いた遺伝子発現方法 | |
| US5917127A (en) | Plasmids useful for and methods of preparing transgenic plants with modifications of habit and yield | |
| EP1036184B1 (en) | Genetic method | |
| AU701718B2 (en) | Processes for modifying plant flowering behaviour | |
| US20040168214A1 (en) | Process for increasing the yield in plants by expressing stably integrated recombinant polypeptides | |
| US20090293142A1 (en) | Methods for increasing shoot-to-root ratio, seed production and resistance to diseases | |
| US20040064851A1 (en) | Transgenic plants which produce isomalt | |
| MXPA00001296A (en) | Processes for increasing the yield in plants | |
| KR100452431B1 (ko) | 강한 구조 유전자 발현을 위한 발현 카세트와 플라스미드 및 그의 사용방법 | |
| CZ2000451A3 (cs) | Způsob zvýšení výnosů rostlin | |
| US6747190B1 (en) | Xyloglucan fucosyltransferase plants transformed with a DNA encoding arabidopsis | |
| US20030150021A1 (en) | Maize 4-alpha-glucanotransferase |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| FZDE | Discontinued |