CA2294037A1 - Methode de determination du genotype d'un organisme par utilisation d'une sonde d'oligonucleotides specifique a l'allele s'hybridant a des regions flanquantes de microsatellites - Google Patents

Methode de determination du genotype d'un organisme par utilisation d'une sonde d'oligonucleotides specifique a l'allele s'hybridant a des regions flanquantes de microsatellites Download PDF

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Publication number
CA2294037A1
CA2294037A1 CA002294037A CA2294037A CA2294037A1 CA 2294037 A1 CA2294037 A1 CA 2294037A1 CA 002294037 A CA002294037 A CA 002294037A CA 2294037 A CA2294037 A CA 2294037A CA 2294037 A1 CA2294037 A1 CA 2294037A1
Authority
CA
Canada
Prior art keywords
sequence
aso
microsatellite
target dna
probe
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002294037A
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English (en)
Inventor
Keith Joseph Edwards
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
University of Bristol
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB9714005A external-priority patent/GB9714005D0/en
Priority claimed from GBGB9806702.8A external-priority patent/GB9806702D0/en
Application filed by University of Bristol filed Critical University of Bristol
Publication of CA2294037A1 publication Critical patent/CA2294037A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne une méthode de détermination du génotype d'un organisme, la méthode consistant à hybrider une sonde d'oligonucléotides spécifique à l'allèle (ASO) à une séquence d'ADN cible, la séquence d'ADN cible comprenant une région d'ADN flanquant une unité de répétition de microsatellite ou une répétition de séquence simple (SSR).
CA002294037A 1997-07-02 1998-07-02 Methode de determination du genotype d'un organisme par utilisation d'une sonde d'oligonucleotides specifique a l'allele s'hybridant a des regions flanquantes de microsatellites Abandoned CA2294037A1 (fr)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
GB9714005A GB9714005D0 (en) 1997-07-02 1997-07-02 Dna
GB9714005.7 1997-07-02
GB9806702.8 1998-03-27
GBGB9806702.8A GB9806702D0 (en) 1998-03-27 1998-03-27 Dna
US8032598P 1998-04-01 1998-04-01
US60/080,325 1998-04-01
PCT/GB1998/001940 WO1999001576A1 (fr) 1997-07-02 1998-07-02 Methode de determination du genotype d'un organisme par utilisation d'une sonde d'oligonucleotides specifique a l'allele s'hybridant a des regions flanquantes de microsatellites

Publications (1)

Publication Number Publication Date
CA2294037A1 true CA2294037A1 (fr) 1999-01-14

Family

ID=27268917

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002294037A Abandoned CA2294037A1 (fr) 1997-07-02 1998-07-02 Methode de determination du genotype d'un organisme par utilisation d'une sonde d'oligonucleotides specifique a l'allele s'hybridant a des regions flanquantes de microsatellites

Country Status (5)

Country Link
EP (1) EP1002127A1 (fr)
AU (1) AU8228098A (fr)
CA (1) CA2294037A1 (fr)
IL (1) IL133794A0 (fr)
WO (1) WO1999001576A1 (fr)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002020844A1 (fr) * 2000-09-08 2002-03-14 Polygenyx, Inc. Methodes et produits relatifs a l'analyse de genotypes a haut rendement
US6861034B1 (en) 2000-11-22 2005-03-01 Xerox Corporation Priming mechanisms for drop ejection devices
US6514704B2 (en) * 2001-02-01 2003-02-04 Xerox Corporation Quality control mechanism and process for a biofluid multi-ejector system
DE10237691B4 (de) * 2002-08-15 2010-01-28 Biotez Berlin-Buch Gmbh Biochemisch-Technologisches Zentrum Verfahren zum Nachweis von Einzelnukleotid-Polymorphismen (SNP) in Genen des Arzneimittelmetabolismus und Testkit zur Durchführung des Verfahrens
EP1590471A4 (fr) 2002-12-31 2009-12-09 Metamorphix Inc Compositions, procédés et systèmes d'inférence concernant des caractéristiques de bovins
CN107974508B (zh) * 2016-10-21 2019-08-20 江苏省中国科学院植物研究所 铁线莲耐热性基因位点qHR2的分子标记方法
CN107974509B (zh) * 2016-10-21 2019-08-20 江苏省中国科学院植物研究所 铁线莲耐热性基因位点qHR3的分子标记方法
CN107988406B (zh) * 2016-10-21 2019-08-20 江苏省中国科学院植物研究所 铁线莲耐热性基因位点qHR4的分子标记方法
CN107385044B (zh) * 2017-07-31 2020-10-27 中国疾病预防控制中心传染病预防控制所 球形孢子丝菌str分子标记及其应用
CN108165614A (zh) * 2018-01-24 2018-06-15 安徽天勤农业科技有限公司 一种育种改进的玉米ssr标记快速银染检测方法
EP4392578A1 (fr) * 2021-09-10 2024-07-03 Cold Spring Harbor Laboratory Procédé de mesure de variations de longueur de microsatellite

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2013430A1 (fr) * 1990-03-29 1991-09-29 Michael Litt Methode d'identification des sujets humains par l'analyse de leur materiel genetique
US5633134A (en) * 1992-10-06 1997-05-27 Ig Laboratories, Inc. Method for simultaneously detecting multiple mutations in a DNA sample
WO1995015400A1 (fr) * 1993-12-03 1995-06-08 The Johns Hopkins University Genotypage par analyse simultanee de multiples loci a microsatellites
DE19525284A1 (de) * 1995-06-28 1997-01-02 Inst Pflanzengenetik & Kultur Mikrosatellitenmarker für Pflanzen der Spezies Triticum aestivum sowie des Tribus Triticeae und ihre Verwendung

Also Published As

Publication number Publication date
IL133794A0 (en) 2001-04-30
EP1002127A1 (fr) 2000-05-24
WO1999001576A1 (fr) 1999-01-14
AU8228098A (en) 1999-01-25

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Legal Events

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FZDE Discontinued