CA2291512A1 - Procedes de fabrication d'une particule de ribonucleoproteine engendrant la nucleotide integrase - Google Patents

Procedes de fabrication d'une particule de ribonucleoproteine engendrant la nucleotide integrase Download PDF

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Publication number
CA2291512A1
CA2291512A1 CA002291512A CA2291512A CA2291512A1 CA 2291512 A1 CA2291512 A1 CA 2291512A1 CA 002291512 A CA002291512 A CA 002291512A CA 2291512 A CA2291512 A CA 2291512A CA 2291512 A1 CA2291512 A1 CA 2291512A1
Authority
CA
Canada
Prior art keywords
intron
group
rna
sequence
nucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002291512A
Other languages
English (en)
Inventor
Alan M. Lambowitz
Georg Mohr
Roland Saldanha
Manabu Matsuura
Clifford James Beall
Jian Yang
Huatao Guo
Steven Zimmerly
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ohio State University Research Foundation
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2291512A1 publication Critical patent/CA2291512A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne des procédés visant à engendrer la nucléotide intégrase par association in vitro d'un ARN à intron du groupe II excisé, ci-après dénommé "ARN exogène", et d'une protéine à codage par intron du groupe II. La production d'ARN exogène résulte de la transcription in vitro d'une molécule d'ADN renfermant une séquence d'intron du groupe II.
CA002291512A 1997-05-28 1998-05-27 Procedes de fabrication d'une particule de ribonucleoproteine engendrant la nucleotide integrase Abandoned CA2291512A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US5092297P 1997-05-28 1997-05-28
US60/050,922 1997-05-28
PCT/US1998/010687 WO1998054353A1 (fr) 1997-05-28 1998-05-27 Procedes de fabrication d'une particule de ribonucleoproteine engendrant la nucleotide integrase

Publications (1)

Publication Number Publication Date
CA2291512A1 true CA2291512A1 (fr) 1998-12-03

Family

ID=21968343

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002291512A Abandoned CA2291512A1 (fr) 1997-05-28 1998-05-27 Procedes de fabrication d'une particule de ribonucleoproteine engendrant la nucleotide integrase

Country Status (5)

Country Link
EP (1) EP1007719A1 (fr)
JP (1) JP2002502247A (fr)
AU (1) AU739106B2 (fr)
CA (1) CA2291512A1 (fr)
WO (1) WO1998054353A1 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6358712B1 (en) 1999-01-05 2002-03-19 Trustee Of Boston University Ordered gene assembly
AU1333401A (en) 1999-10-15 2001-04-30 Ohio State University Research Foundation, The Methods for analyzing the insertion capabilities of modified group ii introns
EP1774036A4 (fr) * 2004-06-14 2008-10-15 Univ Texas At Austin Ciblage de genes dans des cellules eucaryotes par des particules de ribonucleoproteine a intron de groupe ii
GB0612301D0 (en) 2006-06-21 2006-08-02 Morvus Technology Ltd DNA molecules and methods

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5698421A (en) * 1995-09-12 1997-12-16 The Ohio State Research Foundation Ribonucleoprotein particles for cleaving double-stranded DNA and inserting an RNA/DNA molecule into the cleavage site

Also Published As

Publication number Publication date
AU739106B2 (en) 2001-10-04
AU7695498A (en) 1998-12-30
EP1007719A1 (fr) 2000-06-14
WO1998054353A1 (fr) 1998-12-03
JP2002502247A (ja) 2002-01-22

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Legal Events

Date Code Title Description
FZDE Discontinued