CA2287505C - Inhibitors of cellulolytic, xylanolytic and .beta.-glucanolytic enzymes - Google Patents
Inhibitors of cellulolytic, xylanolytic and .beta.-glucanolytic enzymes Download PDFInfo
- Publication number
- CA2287505C CA2287505C CA2287505A CA2287505A CA2287505C CA 2287505 C CA2287505 C CA 2287505C CA 2287505 A CA2287505 A CA 2287505A CA 2287505 A CA2287505 A CA 2287505A CA 2287505 C CA2287505 C CA 2287505C
- Authority
- CA
- Canada
- Prior art keywords
- inhibitor
- wheat
- inhibitor according
- seq
- cereal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000003112 inhibitor Substances 0.000 title claims abstract description 70
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 31
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 31
- 230000001461 cytolytic effect Effects 0.000 title description 5
- 239000000463 material Substances 0.000 claims abstract description 17
- 241000209140 Triticum Species 0.000 claims description 72
- 235000021307 Triticum Nutrition 0.000 claims description 72
- 235000007340 Hordeum vulgare Nutrition 0.000 claims description 42
- 235000013339 cereals Nutrition 0.000 claims description 40
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 claims description 37
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 claims description 37
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 claims description 35
- 108010001817 Endo-1,4-beta Xylanases Proteins 0.000 claims description 31
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 claims description 30
- 241000196324 Embryophyta Species 0.000 claims description 23
- 235000013312 flour Nutrition 0.000 claims description 18
- 238000004519 manufacturing process Methods 0.000 claims description 14
- 125000001429 N-terminal alpha-amino-acid group Chemical group 0.000 claims description 12
- 235000007238 Secale cereale Nutrition 0.000 claims description 8
- 229920002472 Starch Polymers 0.000 claims description 7
- 239000004464 cereal grain Substances 0.000 claims description 6
- 238000004890 malting Methods 0.000 claims description 6
- 239000008107 starch Substances 0.000 claims description 6
- 240000008042 Zea mays Species 0.000 claims description 5
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims description 5
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 5
- 235000009973 maize Nutrition 0.000 claims description 5
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 claims description 3
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 claims description 3
- 240000006394 Sorghum bicolor Species 0.000 claims description 3
- 235000011684 Sorghum saccharatum Nutrition 0.000 claims description 3
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 claims description 3
- 235000013405 beer Nutrition 0.000 claims description 3
- 235000015895 biscuits Nutrition 0.000 claims description 3
- 235000008429 bread Nutrition 0.000 claims description 3
- 235000015496 breakfast cereal Nutrition 0.000 claims description 3
- 235000011868 grain product Nutrition 0.000 claims description 3
- 235000012149 noodles Nutrition 0.000 claims description 3
- 235000015927 pasta Nutrition 0.000 claims description 3
- 238000012545 processing Methods 0.000 claims description 3
- 238000000926 separation method Methods 0.000 claims description 3
- 239000000600 sorbitol Substances 0.000 claims description 3
- 235000019698 starch Nutrition 0.000 claims description 3
- 239000006188 syrup Substances 0.000 claims description 3
- 235000020357 syrup Nutrition 0.000 claims description 3
- 239000000811 xylitol Substances 0.000 claims description 3
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 claims description 3
- 229960002675 xylitol Drugs 0.000 claims description 3
- 235000010447 xylitol Nutrition 0.000 claims description 3
- 235000007319 Avena orientalis Nutrition 0.000 claims description 2
- 244000075850 Avena orientalis Species 0.000 claims description 2
- 240000007594 Oryza sativa Species 0.000 claims description 2
- 235000007164 Oryza sativa Nutrition 0.000 claims description 2
- 244000082988 Secale cereale Species 0.000 claims description 2
- 235000007264 Triticum durum Nutrition 0.000 claims description 2
- 241000209143 Triticum turgidum subsp. durum Species 0.000 claims description 2
- 235000009566 rice Nutrition 0.000 claims description 2
- 240000005979 Hordeum vulgare Species 0.000 claims 2
- 235000019714 Triticale Nutrition 0.000 claims 1
- 241000228158 x Triticosecale Species 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 28
- 238000000034 method Methods 0.000 abstract description 22
- 244000005700 microbiome Species 0.000 abstract description 15
- 230000002829 reductive effect Effects 0.000 abstract description 5
- 230000008569 process Effects 0.000 abstract description 4
- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 description 49
- 241000209219 Hordeum Species 0.000 description 40
- 239000000284 extract Substances 0.000 description 39
- 229920000617 arabinoxylan Polymers 0.000 description 31
- 229940088598 enzyme Drugs 0.000 description 27
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 description 24
- UGXQOOQUZRUVSS-ZZXKWVIFSA-N [5-[3,5-dihydroxy-2-(1,3,4-trihydroxy-5-oxopentan-2-yl)oxyoxan-4-yl]oxy-3,4-dihydroxyoxolan-2-yl]methyl (e)-3-(4-hydroxyphenyl)prop-2-enoate Chemical compound OC1C(OC(CO)C(O)C(O)C=O)OCC(O)C1OC1C(O)C(O)C(COC(=O)\C=C\C=2C=CC(O)=CC=2)O1 UGXQOOQUZRUVSS-ZZXKWVIFSA-N 0.000 description 22
- 230000009467 reduction Effects 0.000 description 17
- 230000005764 inhibitory process Effects 0.000 description 16
- 239000000872 buffer Substances 0.000 description 14
- 102000004169 proteins and genes Human genes 0.000 description 14
- 108090000623 proteins and genes Proteins 0.000 description 14
- 229920001503 Glucan Polymers 0.000 description 10
- 150000004783 arabinoxylans Chemical class 0.000 description 9
- 230000000813 microbial effect Effects 0.000 description 9
- 238000005063 solubilization Methods 0.000 description 9
- 230000007928 solubilization Effects 0.000 description 9
- 241000228245 Aspergillus niger Species 0.000 description 8
- 244000063299 Bacillus subtilis Species 0.000 description 8
- 235000014469 Bacillus subtilis Nutrition 0.000 description 8
- 238000005516 engineering process Methods 0.000 description 8
- 239000012632 extractable Substances 0.000 description 8
- 239000000203 mixture Substances 0.000 description 8
- 239000000758 substrate Substances 0.000 description 8
- 102000003886 Glycoproteins Human genes 0.000 description 7
- 108090000288 Glycoproteins Proteins 0.000 description 7
- 235000013305 food Nutrition 0.000 description 7
- 241000209056 Secale Species 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 6
- 230000007062 hydrolysis Effects 0.000 description 6
- 238000006460 hydrolysis reaction Methods 0.000 description 6
- 229920001221 xylan Polymers 0.000 description 6
- 125000003275 alpha amino acid group Chemical group 0.000 description 5
- 238000000605 extraction Methods 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 5
- 150000004823 xylans Chemical class 0.000 description 5
- SATHPVQTSSUFFW-UHFFFAOYSA-N 4-[6-[(3,5-dihydroxy-4-methoxyoxan-2-yl)oxymethyl]-3,5-dihydroxy-4-methoxyoxan-2-yl]oxy-2-(hydroxymethyl)-6-methyloxane-3,5-diol Chemical compound OC1C(OC)C(O)COC1OCC1C(O)C(OC)C(O)C(OC2C(C(CO)OC(C)C2O)O)O1 SATHPVQTSSUFFW-UHFFFAOYSA-N 0.000 description 4
- 229920000189 Arabinogalactan Polymers 0.000 description 4
- 239000001904 Arabinogalactan Substances 0.000 description 4
- 108091005804 Peptidases Proteins 0.000 description 4
- 239000004365 Protease Substances 0.000 description 4
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 150000001413 amino acids Chemical class 0.000 description 4
- 235000019312 arabinogalactan Nutrition 0.000 description 4
- 230000000593 degrading effect Effects 0.000 description 4
- 239000000499 gel Substances 0.000 description 4
- 239000003550 marker Substances 0.000 description 4
- 235000012054 meals Nutrition 0.000 description 4
- 241000894007 species Species 0.000 description 4
- 239000000725 suspension Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 244000052616 bacterial pathogen Species 0.000 description 3
- 150000001768 cations Chemical class 0.000 description 3
- 210000002421 cell wall Anatomy 0.000 description 3
- 230000003301 hydrolyzing effect Effects 0.000 description 3
- 229920002401 polyacrylamide Polymers 0.000 description 3
- 239000001632 sodium acetate Substances 0.000 description 3
- 235000017281 sodium acetate Nutrition 0.000 description 3
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- YHBDGLZYNIARKJ-GUBZILKMSA-N Ala-Pro-Val Chemical compound CC(C)[C@@H](C(O)=O)NC(=O)[C@@H]1CCCN1C(=O)[C@H](C)N YHBDGLZYNIARKJ-GUBZILKMSA-N 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 208000035240 Disease Resistance Diseases 0.000 description 2
- 229920002684 Sepharose Polymers 0.000 description 2
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 2
- 241000499912 Trichoderma reesei Species 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- -1 alditol acetate Chemical class 0.000 description 2
- 235000013361 beverage Nutrition 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 235000013325 dietary fiber Nutrition 0.000 description 2
- 239000002657 fibrous material Substances 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000006959 non-competitive inhibition Effects 0.000 description 2
- 239000002417 nutraceutical Substances 0.000 description 2
- 235000021436 nutraceutical agent Nutrition 0.000 description 2
- 229920000728 polyester Polymers 0.000 description 2
- 229920001223 polyethylene glycol Polymers 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 229910052709 silver Inorganic materials 0.000 description 2
- 239000004332 silver Substances 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- FRGKKTITADJNOE-UHFFFAOYSA-N sulfanyloxyethane Chemical compound CCOS FRGKKTITADJNOE-UHFFFAOYSA-N 0.000 description 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
- FUKFQILQFQKHLE-DCAQKATOSA-N Ala-Lys-Met Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCSC)C(O)=O FUKFQILQFQKHLE-DCAQKATOSA-N 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 239000004382 Amylase Substances 0.000 description 1
- 235000019890 Amylum Nutrition 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 102000003846 Carbonic anhydrases Human genes 0.000 description 1
- 108090000209 Carbonic anhydrases Proteins 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- 108010084185 Cellulases Proteins 0.000 description 1
- 102000005575 Cellulases Human genes 0.000 description 1
- HGNRJCINZYHNOU-LURJTMIESA-N Lys-Gly Chemical compound NCCCC[C@H](N)C(=O)NCC(O)=O HGNRJCINZYHNOU-LURJTMIESA-N 0.000 description 1
- WPTHAGXMYDRPFD-SRVKXCTJSA-N Met-Lys-Glu Chemical compound CSCC[C@H](N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(O)=O)C(O)=O WPTHAGXMYDRPFD-SRVKXCTJSA-N 0.000 description 1
- 108010058846 Ovalbumin Proteins 0.000 description 1
- 108010065081 Phosphorylase b Proteins 0.000 description 1
- AWJGUZSYVIVZGP-YUMQZZPRSA-N Pro-Val Chemical compound CC(C)[C@@H](C(O)=O)NC(=O)[C@@H]1CCCN1 AWJGUZSYVIVZGP-YUMQZZPRSA-N 0.000 description 1
- XDKKMRPRRCOELJ-GUBZILKMSA-N Pro-Val-Ala Chemical compound OC(=O)[C@H](C)NC(=O)[C@H](C(C)C)NC(=O)[C@@H]1CCCN1 XDKKMRPRRCOELJ-GUBZILKMSA-N 0.000 description 1
- 108091006629 SLC13A2 Proteins 0.000 description 1
- 239000012506 Sephacryl® Substances 0.000 description 1
- VPZKQTYZIVOJDV-LMVFSUKVSA-N Thr-Ala Chemical compound C[C@@H](O)[C@H](N)C(=O)N[C@@H](C)C(O)=O VPZKQTYZIVOJDV-LMVFSUKVSA-N 0.000 description 1
- 241000223261 Trichoderma viride Species 0.000 description 1
- 101710162629 Trypsin inhibitor Proteins 0.000 description 1
- 229940122618 Trypsin inhibitor Drugs 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000003392 amylase inhibitor Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 1
- 229960005070 ascorbic acid Drugs 0.000 description 1
- 235000010323 ascorbic acid Nutrition 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 235000011148 calcium chloride Nutrition 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 230000006957 competitive inhibition Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 238000011033 desalting Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000003467 diminishing effect Effects 0.000 description 1
- MSJMDZAOKORVFC-UAIGNFCESA-L disodium maleate Chemical compound [Na+].[Na+].[O-]C(=O)\C=C/C([O-])=O MSJMDZAOKORVFC-UAIGNFCESA-L 0.000 description 1
- 238000010494 dissociation reaction Methods 0.000 description 1
- 230000005593 dissociations Effects 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 229930182830 galactose Natural products 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 108010002430 hemicellulase Proteins 0.000 description 1
- 239000000416 hydrocolloid Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 108091006086 inhibitor proteins Proteins 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 239000000543 intermediate Substances 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 238000001155 isoelectric focusing Methods 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 108010064235 lysylglycine Proteins 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 229940092253 ovalbumin Drugs 0.000 description 1
- 108010053725 prolylvaline Proteins 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000006965 reversible inhibition Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 239000004458 spent grain Substances 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000007669 thermal treatment Methods 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 239000002753 trypsin inhibitor Substances 0.000 description 1
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- 238000005406 washing Methods 0.000 description 1
- 235000020023 weizenbier Nutrition 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
-
- A—HUMAN NECESSITIES
- A21—BAKING; EDIBLE DOUGHS
- A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
- A21D2/00—Treatment of flour or dough by adding materials thereto before or during baking
- A21D2/08—Treatment of flour or dough by adding materials thereto before or during baking by adding organic substances
- A21D2/24—Organic nitrogen compounds
- A21D2/26—Proteins
- A21D2/264—Vegetable proteins
- A21D2/265—Vegetable proteins from cereals, flour, bran
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
- A23L7/10—Cereal-derived products
- A23L7/109—Types of pasta, e.g. macaroni or noodles
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
- A23L7/10—Cereal-derived products
- A23L7/117—Flakes or other shapes of ready-to-eat type; Semi-finished or partly-finished products therefor
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
- C12C1/00—Preparation of malt
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
- C12C5/00—Other raw materials for the preparation of beer
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
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- C—CHEMISTRY; METALLURGY
- C13—SUGAR INDUSTRY
- C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
- C13K1/00—Glucose; Glucose-containing syrups
- C13K1/06—Glucose; Glucose-containing syrups obtained by saccharification of starch or raw materials containing starch
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Polymers & Plastics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Nutrition Science (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Botany (AREA)
- Emergency Medicine (AREA)
- Animal Husbandry (AREA)
- Gastroenterology & Hepatology (AREA)
- Mycology (AREA)
- Animal Behavior & Ethology (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Public Health (AREA)
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Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP97870060.7 | 1997-04-30 | ||
| EP97870060 | 1997-04-30 | ||
| PCT/EP1998/002590 WO1998049278A1 (en) | 1997-04-30 | 1998-05-04 | INHIBITORS OF CELLULOLYTIC, XYLANOLYTIC AND β-GLUCANOLYTIC ENZYMES |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2287505A1 CA2287505A1 (en) | 1998-11-05 |
| CA2287505C true CA2287505C (en) | 2010-12-07 |
Family
ID=8230998
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2287505A Expired - Lifetime CA2287505C (en) | 1997-04-30 | 1998-05-04 | Inhibitors of cellulolytic, xylanolytic and .beta.-glucanolytic enzymes |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US7393929B1 (pt) |
| EP (1) | EP0996709B1 (pt) |
| JP (1) | JP5044067B2 (pt) |
| CN (1) | CN1174094C (pt) |
| AT (1) | ATE286119T1 (pt) |
| AU (1) | AU751631B2 (pt) |
| BR (1) | BR9809348A (pt) |
| CA (1) | CA2287505C (pt) |
| DE (1) | DE69828405T2 (pt) |
| ES (1) | ES2234119T3 (pt) |
| PT (1) | PT996709E (pt) |
| WO (1) | WO1998049278A1 (pt) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5044067B2 (ja) | 1997-04-30 | 2012-10-10 | ケー．ユー． ルヴァン リサーチ アンド ディヴェロプメント | キシラン分解酵素の阻害剤 |
| US8372620B2 (en) | 1998-12-23 | 2013-02-12 | Dupont Nutrition Biosciences Aps | Bacterial xylanases |
| KR100666307B1 (ko) * | 1998-12-23 | 2007-01-09 | 대니스코 에이/에스 | 단백질 |
| GB9928968D0 (en) * | 1999-12-07 | 2000-02-02 | Danisco | Enzyme |
| GB0001136D0 (en) | 2000-01-18 | 2000-03-08 | Danisco | Process |
| EP2295558B1 (en) | 2000-03-08 | 2017-11-01 | DuPont Nutrition Biosciences ApS | Xylanase variants |
| AU2001268853A1 (en) | 2000-06-22 | 2002-01-02 | K.U. Leuven Research And Development | Biocatalyst inhibitors |
| DE602006015444D1 (de) | 2005-03-22 | 2010-08-26 | Novozymes As | Polypeptide und nukleinsäuren, die diese codieren |
| EP3037528B1 (en) | 2008-12-23 | 2018-08-29 | DuPont Nutrition Biosciences ApS | Polypeptides |
| JP5162051B2 (ja) | 2010-05-19 | 2013-03-13 | サントリーホールディングス株式会社 | 泡の安定化されたビールテイスト飲料 |
| CN110938117B (zh) * | 2019-11-29 | 2021-03-02 | 江南大学 | 一种具有抑制大麦麦芽内源木聚糖酶活力的蛋白及其应用 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9505479D0 (en) | 1995-03-17 | 1995-05-03 | Danisco | Enzyme |
| EP0915985A1 (en) * | 1996-08-05 | 1999-05-19 | Mogen International N.V. | Improved process for the production of alcoholic beverages using maltseed |
| WO1998031816A1 (en) | 1997-01-17 | 1998-07-23 | Regents Of The University Of Minnesota | Dna molecules and protein displaying improved triazine compound degrading ability |
| JP5044067B2 (ja) | 1997-04-30 | 2012-10-10 | ケー．ユー． ルヴァン リサーチ アンド ディヴェロプメント | キシラン分解酵素の阻害剤 |
| EP0979830A1 (en) | 1998-08-12 | 2000-02-16 | Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek Tno | A novel class of xylanase inhibitors |
| KR100666307B1 (ko) | 1998-12-23 | 2007-01-09 | 대니스코 에이/에스 | 단백질 |
| US6090604A (en) | 1999-02-24 | 2000-07-18 | Novo Nordisk Biotech, Inc. | Polypeptides having galactose oxidase activity and nucleic acids encoding same |
| US6096539A (en) | 1999-06-10 | 2000-08-01 | Zeneca Limited | Protein activator of apoptosis |
| AU2001268853A1 (en) | 2000-06-22 | 2002-01-02 | K.U. Leuven Research And Development | Biocatalyst inhibitors |
-
1998
- 1998-05-04 JP JP54662198A patent/JP5044067B2/ja not_active Expired - Lifetime
- 1998-05-04 CA CA2287505A patent/CA2287505C/en not_active Expired - Lifetime
- 1998-05-04 PT PT98925518T patent/PT996709E/pt unknown
- 1998-05-04 ES ES98925518T patent/ES2234119T3/es not_active Expired - Lifetime
- 1998-05-04 AU AU77611/98A patent/AU751631B2/en not_active Expired
- 1998-05-04 US US09/403,625 patent/US7393929B1/en not_active Expired - Fee Related
- 1998-05-04 DE DE69828405T patent/DE69828405T2/de not_active Expired - Lifetime
- 1998-05-04 CN CNB988046806A patent/CN1174094C/zh not_active Expired - Lifetime
- 1998-05-04 WO PCT/EP1998/002590 patent/WO1998049278A1/en active IP Right Grant
- 1998-05-04 AT AT98925518T patent/ATE286119T1/de active
- 1998-05-04 BR BR9809348-7A patent/BR9809348A/pt not_active Application Discontinuation
- 1998-05-04 EP EP98925518A patent/EP0996709B1/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| EP0996709A1 (en) | 2000-05-03 |
| JP2001523104A (ja) | 2001-11-20 |
| DE69828405T2 (de) | 2005-06-02 |
| JP5044067B2 (ja) | 2012-10-10 |
| WO1998049278A1 (en) | 1998-11-05 |
| AU751631B2 (en) | 2002-08-22 |
| CN1254374A (zh) | 2000-05-24 |
| CA2287505A1 (en) | 1998-11-05 |
| EP0996709B1 (en) | 2004-12-29 |
| AU7761198A (en) | 1998-11-24 |
| BR9809348A (pt) | 2000-07-04 |
| PT996709E (pt) | 2005-03-31 |
| ATE286119T1 (de) | 2005-01-15 |
| US7393929B1 (en) | 2008-07-01 |
| ES2234119T3 (es) | 2005-06-16 |
| DE69828405D1 (de) | 2005-02-03 |
| CN1174094C (zh) | 2004-11-03 |
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| MKEX | Expiry |
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