CA2266847A1 - Compositions and methods for enhancing hybridization specificity - Google Patents

Compositions and methods for enhancing hybridization specificity Download PDF

Info

Publication number
CA2266847A1
CA2266847A1 CA002266847A CA2266847A CA2266847A1 CA 2266847 A1 CA2266847 A1 CA 2266847A1 CA 002266847 A CA002266847 A CA 002266847A CA 2266847 A CA2266847 A CA 2266847A CA 2266847 A1 CA2266847 A1 CA 2266847A1
Authority
CA
Canada
Prior art keywords
specificity
composition
oligonucleotide
nucleic acid
cation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002266847A
Other languages
English (en)
French (fr)
Inventor
Jeffrey Van Ness
John Tabone
Lori K. Garrison
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rapigene Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2266847A1 publication Critical patent/CA2266847A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA002266847A 1996-09-24 1997-09-24 Compositions and methods for enhancing hybridization specificity Abandoned CA2266847A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US71913296A 1996-09-24 1996-09-24
US2662196P 1996-09-24 1996-09-24
US08/719,132 1996-09-24
US60/026,621 1996-09-24
PCT/US1997/017413 WO1998013527A2 (en) 1996-09-24 1997-09-24 Compositions and methods for enhancing hybridization specificity

Publications (1)

Publication Number Publication Date
CA2266847A1 true CA2266847A1 (en) 1998-04-02

Family

ID=26701464

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002266847A Abandoned CA2266847A1 (en) 1996-09-24 1997-09-24 Compositions and methods for enhancing hybridization specificity

Country Status (5)

Country Link
EP (1) EP0958378A2 (ja)
JP (1) JP2002514909A (ja)
AU (1) AU4599797A (ja)
CA (1) CA2266847A1 (ja)
WO (1) WO1998013527A2 (ja)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6872521B1 (en) * 1998-06-16 2005-03-29 Beckman Coulter, Inc. Polymerase signaling assay
WO2001023618A2 (en) * 1999-09-30 2001-04-05 Qiagen Genomics, Inc. Compositions and methods for reducing oligonucleotide hybridization and priming specificity
CN1293204C (zh) * 2000-08-30 2007-01-03 戴诺生物技术有限公司 等位基因的测定方法
EP2465943A3 (en) 2001-03-16 2012-10-03 Kalim Mir Linear polymer display
US6753145B2 (en) * 2001-07-05 2004-06-22 Agilent Technologies, Inc. Buffer composition and method for hybridization of microarrays on adsorbed polymer siliceous surfaces
EP1618215A4 (en) * 2003-05-01 2007-12-05 Gen Probe Inc OLIGONUCLEOTIDE AS MOLECULAR SWITCH
CA2494571C (en) * 2003-12-02 2010-02-09 F.Hoffmann-La Roche Ag Oligonucleotides containing molecular rods
KR100601986B1 (ko) * 2005-02-02 2006-07-18 삼성전자주식회사 유전자를 혼성화하는 방법
KR101110013B1 (ko) * 2007-10-05 2012-02-29 (주)바이오니아 서열 내에 어베이직 부분을 포함하는 ｐｃｒ 증폭용프라이머
WO2017041005A1 (en) 2015-09-03 2017-03-09 Abbott Molecular Inc. Hybridization buffers comprising an alkyl diester
WO2017119930A1 (en) * 2016-01-08 2017-07-13 Abbott Molecular Inc. Hybridization buffers comprising guanidinium thiocyanate

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1301606C (en) * 1986-05-02 1992-05-26 David H. Gillespie Chaotropic method for evaluating nucleic acids in a biological sample
DE69030804T2 (de) * 1989-04-05 1997-11-06 Vysis Inc Reagenzien zur verbesserung von hybridisierung
US5232829A (en) * 1989-09-29 1993-08-03 Hoffmann-La Roche Inc. Detection of chlamydia trachomatis by polymerase chain reaction using biotin labelled lina primers and capture probes
EP0529070A1 (en) * 1991-02-27 1993-03-03 Amoco Corporation Methods for improving the sensitivity of hybridization assays
WO1992018649A1 (en) * 1991-04-12 1992-10-29 Microprobe Corporation Compositions and methods for improved extraction and hybridization of nucleic acid
WO1993020234A1 (en) * 1992-03-31 1993-10-14 E.I. Du Pont De Nemours And Company A rapid, high capacity nucleic acid based assay
JPH06192291A (ja) * 1992-09-07 1994-07-12 Nippon Steel Corp 新規ペプチドとそれを用いた血小板凝集抑制剤及び血液凝固抑制剤
AU4848793A (en) * 1992-09-11 1994-04-12 Isis Pharmaceuticals, Inc. Oligonucleotide and nucleotide amine analogs, methods of synthesis and use
US5545626A (en) * 1994-01-19 1996-08-13 The Trustees Of Columbia University In The City Of New York Method of treating glaucoma with oligonucleotides
AU2360195A (en) * 1994-05-05 1995-11-29 Beckman Instruments, Inc. Oligonucleotide repeat arrays

Also Published As

Publication number Publication date
WO1998013527A2 (en) 1998-04-02
JP2002514909A (ja) 2002-05-21
WO1998013527A3 (en) 1998-08-20
AU4599797A (en) 1998-04-17
EP0958378A2 (en) 1999-11-24

Similar Documents

Publication Publication Date Title
US6361940B1 (en) Compositions and methods for enhancing hybridization and priming specificity
US5223414A (en) Process for nucleic acid hybridization and amplification
US6815164B2 (en) Methods and probes for detection and/or quantification of nucleic acid sequences
US6573048B1 (en) Degradable nucleic acid probes and nucleic acid detection methods
US20110003301A1 (en) Methods for detecting genetic variations in dna samples
EP3129505B1 (en) Methods for clonal replication and amplification of nucleic acid molecules for genomic and therapeutic applications
US20060078936A1 (en) Solid support assay systems and methods utilizing non-standard bases
JP2011239790A (ja) 全ゲノム増幅および遺伝型決定のための方法および組成物
JP2007525963A (ja) 全ゲノム増幅および遺伝型決定のための方法および組成物
KR20040036900A (ko) 닉킹제를 사용한 핵산 단편의 증폭
JPH10155500A (ja) 特定のヌクレオチド配列の検出方法及びキット
EP1169474A1 (en) Olignucleotide array and methods of use
AU8772798A (en) Improved detection of mutations in nucleic acids by chemical cleavage
WO2000047766A1 (en) Method for detecting variant nucleotides using arms multiplex amplification
CN105209639B (zh) 在固相载体扩增核酸的方法
US20040019005A1 (en) Methods for parallel measurement of genetic variations
CA2266847A1 (en) Compositions and methods for enhancing hybridization specificity
EP0952228A2 (en) Compositions and methods for enhancing hybridization specificity
US20010044107A1 (en) Recombinase mediated gene chip detection
JP2003159100A (ja) 改良された遺伝子の変異検出方法
EP1055735A2 (en) Method for gene analysis by hybridisation in the presence of a double-stranded DNA-binding protein
EP0852263A1 (en) Method for concentrating variant nucleic acid and nucleic acid concentration test kit for effecting the method
US20040241697A1 (en) Compositions and methods to identify haplotypes
WO2003076665A1 (en) Hybridization assays for gene dosage analysis
AU2001259096A1 (en) Degradable nucleic acid probes and nucleic acid detection methods

Legal Events

Date Code Title Description
FZDE Discontinued