CA2266847A1 - Compositions et procedes pour l'augmentation de la specificite d'hybridation - Google Patents

Compositions et procedes pour l'augmentation de la specificite d'hybridation Download PDF

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Publication number
CA2266847A1
CA2266847A1 CA002266847A CA2266847A CA2266847A1 CA 2266847 A1 CA2266847 A1 CA 2266847A1 CA 002266847 A CA002266847 A CA 002266847A CA 2266847 A CA2266847 A CA 2266847A CA 2266847 A1 CA2266847 A1 CA 2266847A1
Authority
CA
Canada
Prior art keywords
specificity
composition
oligonucleotide
nucleic acid
cation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002266847A
Other languages
English (en)
Inventor
Jeffrey Van Ness
John Tabone
Lori K. Garrison
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rapigene Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2266847A1 publication Critical patent/CA2266847A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Abstract

L'invention concerne des compositions et des procédés permettant d'augmenter la spécificité d'un acide nucléique d'une sonde pour un acide nucléique dans une solution d'hybridation. Un résidu abasique, un résidu de désoxyNébularine ou un hybotrope est utilisé pour augmenter la spécificité. L'invention porte sur un procédé d'identification d'hybotropes utiles, dont des sels, des solvants organiques miscibles avec l'eau, des solvants aprotiques et des solvants organiques, selon des critères d'enthalpie. L'hybridation hybotrope et des olignonucléotides modifiés peuvent être utilisés dans des réactions d'amplification, telles que la PCR, des méthodes de séquençage et de dosage génomiques.
CA002266847A 1996-09-24 1997-09-24 Compositions et procedes pour l'augmentation de la specificite d'hybridation Abandoned CA2266847A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US2662196P 1996-09-24 1996-09-24
US71913296A 1996-09-24 1996-09-24
US60/026,621 1996-09-24
US08/719,132 1996-09-24
PCT/US1997/017413 WO1998013527A2 (fr) 1996-09-24 1997-09-24 Compositions et procedes pour l'augmentation de la specificite d'hybridation

Publications (1)

Publication Number Publication Date
CA2266847A1 true CA2266847A1 (fr) 1998-04-02

Family

ID=26701464

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002266847A Abandoned CA2266847A1 (fr) 1996-09-24 1997-09-24 Compositions et procedes pour l'augmentation de la specificite d'hybridation

Country Status (5)

Country Link
EP (1) EP0958378A2 (fr)
JP (1) JP2002514909A (fr)
AU (1) AU4599797A (fr)
CA (1) CA2266847A1 (fr)
WO (1) WO1998013527A2 (fr)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6872521B1 (en) * 1998-06-16 2005-03-29 Beckman Coulter, Inc. Polymerase signaling assay
WO2001023618A2 (fr) * 1999-09-30 2001-04-05 Qiagen Genomics, Inc. Compositions et methodes destinees a reduire la specificite d'hybridation et d'amorçage des oligonucleotides
AU2001289177A1 (en) * 2000-08-30 2002-03-13 Haplogen, Llc Method for determining alleles
EP2801624B1 (fr) 2001-03-16 2019-03-06 Singular Bio, Inc Puces et procédés d'utilisation
US6753145B2 (en) * 2001-07-05 2004-06-22 Agilent Technologies, Inc. Buffer composition and method for hybridization of microarrays on adsorbed polymer siliceous surfaces
WO2004098386A2 (fr) * 2003-05-01 2004-11-18 Gen-Probe Incorporated Oligonucleotides comprenant un systeme de commutation moleculaire
CA2494571C (fr) * 2003-12-02 2010-02-09 F.Hoffmann-La Roche Ag Oligonucleotides renfermant des tiges moleculaires
KR100601986B1 (ko) * 2005-02-02 2006-07-18 삼성전자주식회사 유전자를 혼성화하는 방법
KR101110013B1 (ko) * 2007-10-05 2012-02-29 (주)바이오니아 서열 내에 어베이직 부분을 포함하는 ｐｃｒ 증폭용프라이머
WO2017041005A1 (fr) 2015-09-03 2017-03-09 Abbott Molecular Inc. Tampons d'hybridation comprenant un diester d'alkyle
US10457981B2 (en) 2016-01-08 2019-10-29 Abbott Molecular Inc. Hybridization buffers

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1301606C (fr) * 1986-05-02 1992-05-26 David H. Gillespie Methode chaotropique pour l'evaluation d'acides nucleiques dans un echantillon biologique
DE69030804T2 (de) * 1989-04-05 1997-11-06 Vysis Inc Reagenzien zur verbesserung von hybridisierung
US5232829A (en) * 1989-09-29 1993-08-03 Hoffmann-La Roche Inc. Detection of chlamydia trachomatis by polymerase chain reaction using biotin labelled lina primers and capture probes
WO1992015708A1 (fr) * 1991-02-27 1992-09-17 Amoco Corporation Procedes d'amelioration de la sensibilite d'essais d'hybridation
WO1992018649A1 (fr) * 1991-04-12 1992-10-29 Microprobe Corporation Compositions et procedes d'extraction et d'hybridation ameliorees d'acide nucleique
WO1993020234A1 (fr) * 1992-03-31 1993-10-14 E.I. Du Pont De Nemours And Company Dosage rapide, a haute capacite, fonde sur l'acide nucleique
JPH06192291A (ja) * 1992-09-07 1994-07-12 Nippon Steel Corp 新規ペプチドとそれを用いた血小板凝集抑制剤及び血液凝固抑制剤
AU4848793A (en) * 1992-09-11 1994-04-12 Isis Pharmaceuticals, Inc. Oligonucleotide and nucleotide amine analogs, methods of synthesis and use
US5545626A (en) * 1994-01-19 1996-08-13 The Trustees Of Columbia University In The City Of New York Method of treating glaucoma with oligonucleotides
EP0758403B1 (fr) * 1994-05-05 1998-06-24 Beckman Instruments, Inc. Groupements repetes d'oligonucleotides

Also Published As

Publication number Publication date
JP2002514909A (ja) 2002-05-21
AU4599797A (en) 1998-04-17
WO1998013527A3 (fr) 1998-08-20
WO1998013527A2 (fr) 1998-04-02
EP0958378A2 (fr) 1999-11-24

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Legal Events

Date Code Title Description
FZDE Discontinued