CA2265887A1 - Toxines et toxoides shiga a marques par l'histidine, fusions de proteines avec ces toxines et toxoides, et leurs procedes de purification et de preparation - Google Patents
Toxines et toxoides shiga a marques par l'histidine, fusions de proteines avec ces toxines et toxoides, et leurs procedes de purification et de preparation Download PDFInfo
- Publication number
- CA2265887A1 CA2265887A1 CA002265887A CA2265887A CA2265887A1 CA 2265887 A1 CA2265887 A1 CA 2265887A1 CA 002265887 A CA002265887 A CA 002265887A CA 2265887 A CA2265887 A CA 2265887A CA 2265887 A1 CA2265887 A1 CA 2265887A1
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- Prior art keywords
- shiga
- toxin
- toxins
- toxoids
- polypeptide
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- C07K14/245—Escherichia (G)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- C07K14/25—Shigella (G)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Veterinary Medicine (AREA)
- Communicable Diseases (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oncology (AREA)
- Public Health (AREA)
- General Chemical & Material Sciences (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
L'invention concerne l'isolement et la purification de toxines Shiga à activité biologique et immunologique, marquées par l'histine, une toxine associée à la colite hémorragique et à des séquelles du syndrome hémolytique et urémique potentiellement mortelles transmis par des souches de bactéries pathogènes. L'invention décrit comment ce marquage par l'histidine simplifie et accélère grandement la purification des toxines Shiga ainsi qu'un procédé amélioré pour effectuer ladite purification. L'un des aspects de l'invention porte sur l'obtention et l'utilisation de toxoïdes Shiga immunoréactifs mais non toxiques. Un autre aspect de l'invention porte sur la production et l'utilisation de protéines fusionnées à des toxines ou toxoïdes Shiga marqués par l'histine. L'invention porte en outre sur la production et sur l'utilisation d'anticorps anti toxines ou toxoïdes Shiga marqués par l'histine ou anti protéines fusionnées à des toxines ou toxoïdes Shiga.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US2563796P | 1996-09-10 | 1996-09-10 | |
| US60/025,637 | 1996-09-10 | ||
| PCT/US1997/015836 WO1998011229A2 (fr) | 1996-09-10 | 1997-09-09 | Toxines et toxoides shiga a marques par l'histidine, fusions de proteines avec ces toxines et toxoides, et leurs procedes de purification et de preparation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2265887A1 true CA2265887A1 (fr) | 1998-03-19 |
Family
ID=21827219
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002265887A Abandoned CA2265887A1 (fr) | 1996-09-10 | 1997-09-09 | Toxines et toxoides shiga a marques par l'histidine, fusions de proteines avec ces toxines et toxoides, et leurs procedes de purification et de preparation |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP0929679A2 (fr) |
| JP (1) | JP2001500730A (fr) |
| AR (1) | AR010218A1 (fr) |
| AU (1) | AU4184597A (fr) |
| CA (1) | CA2265887A1 (fr) |
| WO (1) | WO1998011229A2 (fr) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2766193B1 (fr) | 1997-07-18 | 2001-09-14 | Inst Curie | Polypeptide chimerique comprenant le fragment b de la toxine shiga et des peptides d'interet therapeutique |
| GB9715177D0 (en) * | 1997-07-21 | 1997-09-24 | Neutec Pharma Plc | Medicament |
| JP4820000B2 (ja) * | 1998-05-15 | 2011-11-24 | アラン エム グリーン | 免疫処置のためのベロ毒素ｂサブユニット |
| EP1057895A1 (fr) * | 1999-06-04 | 2000-12-06 | Lohmann Animal Health GmbH & Co. KG | Protéine de fusion comprenant le fragment B de la toxine de Shiga, préparation (vaccinale) la comprenant, et procédé pour leur préparation |
| ES2532985T3 (es) * | 2006-02-16 | 2015-04-06 | The Henry M. Jackson Foundation For The Advancement Of Military Medicine, Inc. | Proteínas quiméricas de toxoide de Shiga |
| WO2007124133A2 (fr) * | 2006-04-20 | 2007-11-01 | The Henry M. Jackson Foundation For The Advancement Of Military Medicine Incorporated | Procédés et compositions à base de la protéine toxine de shiga de type 1 |
| PE20120425A1 (es) | 2009-01-23 | 2012-05-03 | Jackson H M Found Military Med | Metodos y composiciones en base a la proteina tipo 2 de la toxina shiga |
| EP3666795A1 (fr) * | 2013-03-12 | 2020-06-17 | Molecular Templates, Inc. | Protéines cytotoxiques comprenant des régions de liaison de ciblage de cellules et des régions d'une sous-unité de la toxine de shiga pour la mort sélective de types de cellules spécifiques |
| KR102692208B1 (ko) | 2014-01-27 | 2024-08-06 | 몰레큘러 템플레이츠, 인코퍼레이션. | 폴리펩티드를 전달하는 mhc 클래스 i 항원결정기 |
| ES2723774T3 (es) * | 2014-03-11 | 2019-09-02 | Molecular Templates Inc | Proteínas que comprenden regiones de unión, regiones efectoras de la subunidad A de toxina Shiga y motivos señal de localización de retículo endoplasmático carboxi terminal |
| US11142584B2 (en) | 2014-03-11 | 2021-10-12 | Molecular Templates, Inc. | CD20-binding proteins comprising Shiga toxin A subunit effector regions for inducing cellular internalization and methods using same |
| KR20160127759A (ko) * | 2014-03-11 | 2016-11-04 | 몰레큘러 템플레이츠, 인코퍼레이션. | 아미노-말단 근위 시가 독소 a 서브유닛 작동체 영역 및 세포-표적 면역글로불린-유형 결합 영역을 포함하는 단백질 |
| IL286804B (en) | 2014-06-11 | 2022-08-01 | Molecular Templates Inc | Polypeptides of cleavage-resistant protease, activator subunit shiga toxin, and cell-targeting molecules containing them |
| MX2017010072A (es) | 2015-02-05 | 2017-11-09 | Molecular Templates Inc | Moleculas multivalentes que se enlazan a cd20, las cuales comprenden regiones efectoras de la subunidad a de la toxina shiga, y composiciones enriquecidas de las mismas. |
| CA2984635A1 (fr) | 2015-05-30 | 2016-12-08 | Molecular Templates, Inc. | Supports de sous-unite a de toxine de shiga, deimmunises, et molecules de ciblage de cellule les comprenant |
| IL302130A (en) | 2016-12-07 | 2023-06-01 | Molecular Templates Inc | Shiga toxin A subunit activator polypeptides, Shiga toxin activator scaffolds and cell-targeting molecules for site-specific conjugation |
| KR102590672B1 (ko) | 2017-01-25 | 2023-10-18 | 몰레큘러 템플레이츠, 인코퍼레이션. | 탈면역된 시가 독소 a 서브유닛 이펙터 및 cd8+ t-세포 에피토프를 포함하는 세포-표적화 분자 |
| CA3097178A1 (fr) | 2018-04-17 | 2019-10-24 | Molecular Templates, Inc. | Molecules ciblant her2 comprenant des matrices de la sous-unite a, rendue non immunogene, de la shigatoxine |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NZ337543A (en) * | 1994-10-24 | 2002-06-28 | Allergan Inc | Composition comprising avian antitoxin against clostridial neurotoxin protein and method for producing recombinant antitoxin proteins are provided for |
| JPH11506424A (ja) * | 1995-03-24 | 1999-06-08 | オフィディアン ファーマシューティカルズ インコーポレーテッド | ベロ毒素産生大腸菌に対する治療 |
-
1997
- 1997-09-09 EP EP97939845A patent/EP0929679A2/fr not_active Withdrawn
- 1997-09-09 AU AU41845/97A patent/AU4184597A/en not_active Abandoned
- 1997-09-09 AR ARP970104115A patent/AR010218A1/es unknown
- 1997-09-09 JP JP10513748A patent/JP2001500730A/ja active Pending
- 1997-09-09 WO PCT/US1997/015836 patent/WO1998011229A2/fr not_active Application Discontinuation
- 1997-09-09 CA CA002265887A patent/CA2265887A1/fr not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| JP2001500730A (ja) | 2001-01-23 |
| WO1998011229A3 (fr) | 1998-04-16 |
| WO1998011229A2 (fr) | 1998-03-19 |
| AU4184597A (en) | 1998-04-02 |
| AR010218A1 (es) | 2000-06-07 |
| EP0929679A2 (fr) | 1999-07-21 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Discontinued |