CA2261989C - Recombinant pox virus for immunization against tumor-associated antigens - Google Patents
Recombinant pox virus for immunization against tumor-associated antigens Download PDFInfo
- Publication number
- CA2261989C CA2261989C CA002261989A CA2261989A CA2261989C CA 2261989 C CA2261989 C CA 2261989C CA 002261989 A CA002261989 A CA 002261989A CA 2261989 A CA2261989 A CA 2261989A CA 2261989 C CA2261989 C CA 2261989C
- Authority
- CA
- Canada
- Prior art keywords
- recombinant
- virus
- pox virus
- gene
- cea
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 241000700605 Viruses Species 0.000 title claims abstract description 107
- 206010028980 Neoplasm Diseases 0.000 title claims abstract description 50
- 239000000427 antigen Substances 0.000 title claims abstract description 40
- 102000036639 antigens Human genes 0.000 title claims abstract description 40
- 108091007433 antigens Proteins 0.000 title claims abstract description 40
- 230000003053 immunization Effects 0.000 title description 8
- 238000002649 immunization Methods 0.000 title description 8
- 108010072866 Prostate-Specific Antigen Proteins 0.000 claims description 19
- 108010022366 Carcinoembryonic Antigen Proteins 0.000 claims description 16
- 229940032310 PROSTVAC vaccine Drugs 0.000 claims description 8
- 239000003814 drug Substances 0.000 claims description 5
- 101100346932 Mus musculus Muc1 gene Proteins 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims 4
- 102100038358 Prostate-specific antigen Human genes 0.000 claims 3
- 102000012406 Carcinoembryonic Antigen Human genes 0.000 claims 2
- 230000028993 immune response Effects 0.000 abstract description 8
- 230000000763 evoking effect Effects 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 description 61
- 239000013612 plasmid Substances 0.000 description 30
- 210000004027 cell Anatomy 0.000 description 28
- 108020004414 DNA Proteins 0.000 description 27
- 206010046865 Vaccinia virus infection Diseases 0.000 description 25
- 208000007089 vaccinia Diseases 0.000 description 25
- 239000013598 vector Substances 0.000 description 22
- 102000007066 Prostate-Specific Antigen Human genes 0.000 description 15
- 238000000034 method Methods 0.000 description 15
- 102100025475 Carcinoembryonic antigen-related cell adhesion molecule 5 Human genes 0.000 description 14
- 239000012634 fragment Substances 0.000 description 14
- 230000003612 virological effect Effects 0.000 description 14
- 201000011510 cancer Diseases 0.000 description 13
- 229960005486 vaccine Drugs 0.000 description 13
- 241000700618 Vaccinia virus Species 0.000 description 12
- 238000010276 construction Methods 0.000 description 12
- 238000001727 in vivo Methods 0.000 description 12
- 102000004169 proteins and genes Human genes 0.000 description 12
- 230000014509 gene expression Effects 0.000 description 10
- 238000003780 insertion Methods 0.000 description 10
- 230000037431 insertion Effects 0.000 description 10
- 235000018102 proteins Nutrition 0.000 description 10
- 230000006798 recombination Effects 0.000 description 10
- 238000005215 recombination Methods 0.000 description 10
- 241000699670 Mus sp. Species 0.000 description 9
- 241000588724 Escherichia coli Species 0.000 description 8
- 101150066555 lacZ gene Proteins 0.000 description 8
- 108091028043 Nucleic acid sequence Proteins 0.000 description 7
- 108020004440 Thymidine kinase Proteins 0.000 description 7
- 101150045267 CEA gene Proteins 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 6
- 108090000790 Enzymes Proteins 0.000 description 6
- 108010010995 MART-1 Antigen Proteins 0.000 description 6
- 102100028389 Melanoma antigen recognized by T-cells 1 Human genes 0.000 description 6
- 102000006601 Thymidine Kinase Human genes 0.000 description 6
- 239000002671 adjuvant Substances 0.000 description 6
- 239000003550 marker Substances 0.000 description 6
- 239000002609 medium Substances 0.000 description 6
- 210000003819 peripheral blood mononuclear cell Anatomy 0.000 description 6
- 108090000765 processed proteins & peptides Proteins 0.000 description 6
- 230000010076 replication Effects 0.000 description 6
- 210000004881 tumor cell Anatomy 0.000 description 6
- 239000013603 viral vector Substances 0.000 description 6
- 102000003886 Glycoproteins Human genes 0.000 description 5
- 108090000288 Glycoproteins Proteins 0.000 description 5
- 238000003556 assay Methods 0.000 description 5
- 230000006801 homologous recombination Effects 0.000 description 5
- 238000002744 homologous recombination Methods 0.000 description 5
- 208000015181 infectious disease Diseases 0.000 description 5
- 239000013600 plasmid vector Substances 0.000 description 5
- 230000002103 transcriptional effect Effects 0.000 description 5
- 241000700663 Avipoxvirus Species 0.000 description 4
- 102000004127 Cytokines Human genes 0.000 description 4
- 108090000695 Cytokines Proteins 0.000 description 4
- 208000000666 Fowlpox Diseases 0.000 description 4
- 241000700662 Fowlpox virus Species 0.000 description 4
- 108010002350 Interleukin-2 Proteins 0.000 description 4
- 102000000588 Interleukin-2 Human genes 0.000 description 4
- 241000700629 Orthopoxvirus Species 0.000 description 4
- 210000001744 T-lymphocyte Anatomy 0.000 description 4
- 230000003115 biocidal effect Effects 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 210000001151 cytotoxic T lymphocyte Anatomy 0.000 description 4
- 230000002163 immunogen Effects 0.000 description 4
- 239000002955 immunomodulating agent Substances 0.000 description 4
- 229940121354 immunomodulator Drugs 0.000 description 4
- 238000009169 immunotherapy Methods 0.000 description 4
- 238000001802 infusion Methods 0.000 description 4
- 230000001404 mediated effect Effects 0.000 description 4
- 201000001441 melanoma Diseases 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- LINMATFDVHBYOS-MBJXGIAVSA-N (2s,3r,4s,5r,6r)-2-[(5-bromo-1h-indol-3-yl)oxy]-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1OC1=CNC2=CC=C(Br)C=C12 LINMATFDVHBYOS-MBJXGIAVSA-N 0.000 description 3
- 241000271566 Aves Species 0.000 description 3
- 201000009030 Carcinoma Diseases 0.000 description 3
- 108091026890 Coding region Proteins 0.000 description 3
- 102000053602 DNA Human genes 0.000 description 3
- 241001529936 Murinae Species 0.000 description 3
- 108010008038 Synthetic Vaccines Proteins 0.000 description 3
- 230000024932 T cell mediated immunity Effects 0.000 description 3
- 241000700647 Variola virus Species 0.000 description 3
- 108020005202 Viral DNA Proteins 0.000 description 3
- 210000000612 antigen-presenting cell Anatomy 0.000 description 3
- 239000003593 chromogenic compound Substances 0.000 description 3
- 230000000875 corresponding effect Effects 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 210000000987 immune system Anatomy 0.000 description 3
- 229940124551 recombinant vaccine Drugs 0.000 description 3
- 230000004044 response Effects 0.000 description 3
- 108091008146 restriction endonucleases Proteins 0.000 description 3
- 230000035899 viability Effects 0.000 description 3
- 208000030507 AIDS Diseases 0.000 description 2
- 241000700664 Capripoxvirus Species 0.000 description 2
- 101001002657 Homo sapiens Interleukin-2 Proteins 0.000 description 2
- 206010060862 Prostate cancer Diseases 0.000 description 2
- 208000000236 Prostatic Neoplasms Diseases 0.000 description 2
- 230000006023 anti-tumor response Effects 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 210000003837 chick embryo Anatomy 0.000 description 2
- 231100000433 cytotoxic Toxicity 0.000 description 2
- 230000001472 cytotoxic effect Effects 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- -1 e.g. Proteins 0.000 description 2
- 238000003018 immunoassay Methods 0.000 description 2
- 230000001024 immunotherapeutic effect Effects 0.000 description 2
- 238000011065 in-situ storage Methods 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 231100000518 lethal Toxicity 0.000 description 2
- 230000001665 lethal effect Effects 0.000 description 2
- 210000004698 lymphocyte Anatomy 0.000 description 2
- 239000002773 nucleotide Substances 0.000 description 2
- 125000003729 nucleotide group Chemical group 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 210000002307 prostate Anatomy 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 238000003156 radioimmunoprecipitation Methods 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 229940083538 smallpox vaccine Drugs 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 238000011282 treatment Methods 0.000 description 2
- 210000003171 tumor-infiltrating lymphocyte Anatomy 0.000 description 2
- 241001430294 unidentified retrovirus Species 0.000 description 2
- 238000002255 vaccination Methods 0.000 description 2
- 230000001018 virulence Effects 0.000 description 2
- 208000010507 Adenocarcinoma of Lung Diseases 0.000 description 1
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
- 102100026189 Beta-galactosidase Human genes 0.000 description 1
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- 241000282693 Cercopithecidae Species 0.000 description 1
- 206010009944 Colon cancer Diseases 0.000 description 1
- 241000272201 Columbiformes Species 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- YVGGHNCTFXOJCH-UHFFFAOYSA-N DDT Chemical compound C1=CC(Cl)=CC=C1C(C(Cl)(Cl)Cl)C1=CC=C(Cl)C=C1 YVGGHNCTFXOJCH-UHFFFAOYSA-N 0.000 description 1
- 108020003215 DNA Probes Proteins 0.000 description 1
- 239000003298 DNA probe Substances 0.000 description 1
- 208000006586 Ectromelia Diseases 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- 108700028146 Genetic Enhancer Elements Proteins 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 101100321817 Human parvovirus B19 (strain HV) 7.5K gene Proteins 0.000 description 1
- 108010065805 Interleukin-12 Proteins 0.000 description 1
- 108090000172 Interleukin-15 Proteins 0.000 description 1
- 108090001005 Interleukin-6 Proteins 0.000 description 1
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 1
- 241000700563 Leporipoxvirus Species 0.000 description 1
- 206010024503 Limb reduction defect Diseases 0.000 description 1
- 241000282560 Macaca mulatta Species 0.000 description 1
- 241000712079 Measles morbillivirus Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000700627 Monkeypox virus Species 0.000 description 1
- 108700020796 Oncogene Proteins 0.000 description 1
- 102000043276 Oncogene Human genes 0.000 description 1
- 108700026244 Open Reading Frames Proteins 0.000 description 1
- 238000009004 PCR Kit Methods 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- 241000700625 Poxviridae Species 0.000 description 1
- 239000012980 RPMI-1640 medium Substances 0.000 description 1
- 241000700564 Rabbit fibroma virus Species 0.000 description 1
- 206010037742 Rabies Diseases 0.000 description 1
- 241000711798 Rabies lyssavirus Species 0.000 description 1
- 241000700638 Raccoonpox virus Species 0.000 description 1
- 231100000645 Reed–Muench method Toxicity 0.000 description 1
- 241000287219 Serinus canaria Species 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 241000700568 Suipoxvirus Species 0.000 description 1
- 241000700565 Swinepox virus Species 0.000 description 1
- 230000005867 T cell response Effects 0.000 description 1
- 101150003725 TK gene Proteins 0.000 description 1
- 206010053613 Type IV hypersensitivity reaction Diseases 0.000 description 1
- 108010059722 Viral Fusion Proteins Proteins 0.000 description 1
- 241001536558 Yaba monkey tumor virus Species 0.000 description 1
- DVKFVGVMPLXLKC-PUGXJXRHSA-N [(2s,3r,4s,5s,6r)-2-[(2s,3s,4s,5r)-3,4-dihydroxy-2,5-bis(hydroxymethyl)oxolan-2-yl]-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl] dihydrogen phosphate Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@]1(CO)[C@@]1(OP(O)(O)=O)[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 DVKFVGVMPLXLKC-PUGXJXRHSA-N 0.000 description 1
- 229940024606 amino acid Drugs 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000002494 anti-cea effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 238000011398 antitumor immunotherapy Methods 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000007900 aqueous suspension Substances 0.000 description 1
- 229960001230 asparagine Drugs 0.000 description 1
- 235000009582 asparagine Nutrition 0.000 description 1
- 230000003416 augmentation Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 108010005774 beta-Galactosidase Proteins 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 201000008275 breast carcinoma Diseases 0.000 description 1
- 238000002619 cancer immunotherapy Methods 0.000 description 1
- 229940022399 cancer vaccine Drugs 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000013592 cell lysate Substances 0.000 description 1
- 239000013553 cell monolayer Substances 0.000 description 1
- 238000002659 cell therapy Methods 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 238000007398 colorimetric assay Methods 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000000139 costimulatory effect Effects 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003937 drug carrier Substances 0.000 description 1
- 230000002500 effect on skin Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 230000008029 eradication Effects 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000010195 expression analysis Methods 0.000 description 1
- 230000001605 fetal effect Effects 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 108020001507 fusion proteins Proteins 0.000 description 1
- 102000037865 fusion proteins Human genes 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 229930195712 glutamate Natural products 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- 210000003714 granulocyte Anatomy 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 102000007579 human kallikrein-related peptidase 3 Human genes 0.000 description 1
- 108010071652 human kallikrein-related peptidase 3 Proteins 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000000984 immunochemical effect Effects 0.000 description 1
- 230000002584 immunomodulator Effects 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 238000007918 intramuscular administration Methods 0.000 description 1
- 238000001990 intravenous administration Methods 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 230000001589 lymphoproliferative effect Effects 0.000 description 1
- 230000003211 malignant effect Effects 0.000 description 1
- 210000002752 melanocyte Anatomy 0.000 description 1
- 230000001394 metastastic effect Effects 0.000 description 1
- 206010061289 metastatic neoplasm Diseases 0.000 description 1
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 208000005871 monkeypox Diseases 0.000 description 1
- UXOUKMQIEVGVLY-UHFFFAOYSA-N morin Natural products OC1=CC(O)=CC(C2=C(C(=O)C3=C(O)C=C(O)C=C3O2)O)=C1 UXOUKMQIEVGVLY-UHFFFAOYSA-N 0.000 description 1
- 231100000956 nontoxicity Toxicity 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 231100000590 oncogenic Toxicity 0.000 description 1
- 230000002246 oncogenic effect Effects 0.000 description 1
- 238000007911 parenteral administration Methods 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 238000012809 post-inoculation Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 201000005825 prostate adenocarcinoma Diseases 0.000 description 1
- 210000004908 prostatic fluid Anatomy 0.000 description 1
- 238000011536 re-plating Methods 0.000 description 1
- 210000001525 retina Anatomy 0.000 description 1
- 238000012340 reverse transcriptase PCR Methods 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000012679 serum free medium Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 238000001890 transfection Methods 0.000 description 1
- 230000005951 type IV hypersensitivity Effects 0.000 description 1
- 208000027930 type IV hypersensitivity disease Diseases 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 125000001493 tyrosinyl group Chemical group [H]OC1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P13/00—Drugs for disorders of the urinary system
- A61P13/08—Drugs for disorders of the urinary system of the prostate
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/70503—Immunoglobulin superfamily
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/24011—Poxviridae
- C12N2710/24041—Use of virus, viral particle or viral elements as a vector
- C12N2710/24043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Virology (AREA)
- Animal Behavior & Ethology (AREA)
- Toxicology (AREA)
- Cell Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Gastroenterology & Hepatology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- Public Health (AREA)
- Urology & Nephrology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US68628096A | 1996-07-25 | 1996-07-25 | |
| US08/686,280 | 1996-07-25 | ||
| PCT/US1997/012203 WO1998004727A1 (en) | 1996-07-25 | 1997-07-15 | Recombinant pox virus for immunization against tumor-associated antigens |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2261989A1 CA2261989A1 (en) | 1998-02-05 |
| CA2261989C true CA2261989C (en) | 2008-09-30 |
Family
ID=24755674
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002261989A Expired - Fee Related CA2261989C (en) | 1996-07-25 | 1997-07-15 | Recombinant pox virus for immunization against tumor-associated antigens |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US7410644B2 (en:Method) |
| EP (2) | EP2112225A1 (en:Method) |
| JP (2) | JP4475683B2 (en:Method) |
| AU (1) | AU731860B2 (en:Method) |
| CA (1) | CA2261989C (en:Method) |
| WO (1) | WO1998004727A1 (en:Method) |
Families Citing this family (44)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2245778T3 (es) * | 1993-08-11 | 2006-01-16 | Jenner Technologies | Vacuna contra el cancer de prostata. |
| US20010036928A1 (en) * | 1996-04-22 | 2001-11-01 | Chamberlain Ronald S. | Heterologous boosting immunizations |
| US6969609B1 (en) | 1998-12-09 | 2005-11-29 | The United States Of America As Represented By The Department Of Health And Human Serivces | Recombinant vector expressing multiple costimulatory molecules and uses thereof |
| US7148035B1 (en) | 1998-11-18 | 2006-12-12 | Oxford Biomedica (Uk) Limited | Polypeptide |
| EP1036091B2 (en) * | 1998-11-18 | 2008-03-26 | Oxford Biomedica (UK) Limited | 5t4 tumour-associated antigen for use in tumour immunotherapy |
| EP1865065A1 (en) * | 1998-12-09 | 2007-12-12 | The Government of the United States of America, as represented by the Secretary, Department of Health and Human Services | A recombinant vector expressing multiple costimulatory molecules and uses thereof |
| DK1137792T3 (da) * | 1998-12-09 | 2007-09-10 | Us Gov Health & Human Serv | En rekombinant vektor, der udtrykker adskillige costimulatoriske molekyler, og anvendelser deraf |
| ATE369895T1 (de) * | 2002-04-09 | 2007-09-15 | Sanofi Pasteur Ltd | Modifizierte cea nucleinsäure und expressionsvektoren |
| CA2536317A1 (en) * | 2003-08-21 | 2005-03-03 | Virax Development Pty Ltd | Poxvirus vector encoding prostate specific antigens for treatment of prostate cancer |
| US8562970B2 (en) * | 2003-10-08 | 2013-10-22 | Sanofi Pasteur Limited | Modified CEA/B7 vector |
| ES2476990T3 (es) * | 2003-11-12 | 2014-07-15 | The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Sistema para tratar y prevenir cáncer de mama |
| US8901093B2 (en) | 2003-11-12 | 2014-12-02 | The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services | Custom vectors for treating and preventing pancreatic cancer |
| CN102281892A (zh) | 2009-01-13 | 2011-12-14 | 特朗斯吉有限公司 | 酿酒酵母线粒体核酸级分用于免疫刺激的用途 |
| SG196837A1 (en) | 2009-01-20 | 2014-02-13 | Transgene Sa | Soluble icam-1 as biomarker for prediction of therapeutic response |
| DK2411815T3 (en) | 2009-03-24 | 2015-11-30 | Transgene Sa | Biomarker MONITORING OF PATIENTS |
| US20120058493A1 (en) | 2009-04-17 | 2012-03-08 | Bruce Acres | Biomarker for monitoring patients |
| JP5650212B2 (ja) | 2009-07-10 | 2015-01-07 | トランジェーヌ、ソシエテ、アノニムＴｒａｎｓｇｅｎｅ Ｓ．Ａ． | 患者を選択するためのバイオマーカーおよび関連方法 |
| US9804163B2 (en) | 2010-08-06 | 2017-10-31 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Biomarkers for prostate cancer and methods for their detection |
| US9725494B2 (en) * | 2013-05-17 | 2017-08-08 | United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Soluble CD27 (sCD27) and the use thereof |
| US20160271239A1 (en) * | 2013-11-05 | 2016-09-22 | Bavarian Nordic A/S | Combination Therapy for Treating Cancer with a Poxvirus Expressing a Tumor Antigen and an Antagonist and/or Agonist of an Immune Checkpoint Inhibitor |
| DK3142689T3 (da) | 2014-05-13 | 2021-02-15 | Bavarian Nordic As | Kombinationsbehandling til behandling af cancer med en koppevirus, som udtrykker et tumorantigen, og et monoklonalt antistof mod tim-3 |
| KR102504758B1 (ko) | 2014-07-16 | 2023-02-28 | 트랜스진 | 종양 세포 붕괴성 바이러스와 면역 체크포인트 조절제의 배합물 |
| WO2016128542A1 (en) | 2015-02-13 | 2016-08-18 | Transgene Sa | Immunotherapeutic vaccine and antibody combination therapy |
| JP6851364B2 (ja) | 2015-07-31 | 2021-03-31 | バヴァリアン・ノルディック・アクティーゼルスカブ | ポックスウイルスにおける発現を増強するためのプロモーター |
| EP3400009A2 (en) | 2016-01-05 | 2018-11-14 | The U.S.A. As Represented By The Secretary, Department Of Health And Human Services | Combination of histone deacetylase inhibitor and immunotherapy |
| EP3407912B1 (en) | 2016-01-28 | 2022-05-18 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Methods for enhancing the potency of the immune checkpoint inhibitors |
| WO2017136342A1 (en) | 2016-02-02 | 2017-08-10 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Fulvestrant for inducing immune-mediated cytotoxic lysis of cancer cells |
| EP3452081A1 (en) | 2016-05-04 | 2019-03-13 | Transgene SA | Combination therapy with cpg tlr9 ligand |
| AU2017336269B2 (en) | 2016-09-28 | 2022-06-16 | Bavarian Nordic A/S | Compositions and methods for enhancing the stability of transgenes in poxviruses |
| WO2018069316A2 (en) | 2016-10-10 | 2018-04-19 | Transgene Sa | Immunotherapeutic product and mdsc modulator combination therapy |
| WO2018091680A1 (en) | 2016-11-18 | 2018-05-24 | Transgene Sa | Cowpox-based oncolytic vectors |
| JP7110203B2 (ja) | 2016-12-28 | 2022-08-01 | トランジェーヌ | 腫瘍溶解性ウイルスおよび治療用分子 |
| SG11201909516VA (en) | 2017-04-14 | 2019-11-28 | Tollnine Inc | Immunomodulating polynucleotides, antibody conjugates thereof, and methods of their use |
| US11052147B2 (en) | 2017-05-15 | 2021-07-06 | Janssen Vaccines & Prevention B.V. | Stable virus-containing composition |
| WO2018210804A1 (en) | 2017-05-15 | 2018-11-22 | Janssen Vaccines & Prevention B.V. | Stable virus-containing composition |
| WO2019020543A1 (en) | 2017-07-28 | 2019-01-31 | Transgene Sa | ONCOLYTIC VIRUSES EXPRESSING AGENTS TARGETING METABOLIC IMMUNE MODULATORS |
| US11273211B2 (en) | 2017-08-24 | 2022-03-15 | Bavarian Nordic A/S | Combination therapy for treating cancer with an intravenous administration of a recombinant MVA and an antibody |
| JP2021516957A (ja) | 2018-03-07 | 2021-07-15 | トランジェーヌＴｒａｎｓｇｅｎｅ | パラポックスウイルスベクター |
| WO2020011754A1 (en) | 2018-07-09 | 2020-01-16 | Transgene | Chimeric vaccinia viruses |
| US12150988B2 (en) | 2018-09-06 | 2024-11-26 | Bavarian Nordic A/S | Storage improved poxvirus compositions |
| WO2020240024A1 (en) | 2019-05-29 | 2020-12-03 | Université De Tours | Toxoplasma platform for treating cancer |
| WO2021174091A1 (en) | 2020-02-28 | 2021-09-02 | Tallac Therapeutics, Inc. | Transglutaminase-mediated conjugation |
| IL296250A (en) | 2020-03-12 | 2022-11-01 | Bavarian Nordic As | Compositions improving poxvirus stability |
| EP4573113A1 (en) | 2022-08-18 | 2025-06-25 | Transgene | Chimeric poxviruses |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4603112A (en) | 1981-12-24 | 1986-07-29 | Health Research, Incorporated | Modified vaccinia virus |
| US5833975A (en) * | 1989-03-08 | 1998-11-10 | Virogenetics Corporation | Canarypox virus expressing cytokine and/or tumor-associated antigen DNA sequence |
| US5174993A (en) * | 1981-12-24 | 1992-12-29 | Health Research Inc. | Recombinant avipox virus and immunological use thereof |
| DK354487A (da) * | 1986-07-11 | 1988-01-12 | Noboru Yanaihara | Oncogen-relaterede peptider |
| US5266313A (en) * | 1987-02-03 | 1993-11-30 | The United States Of America As Represented By The Department Of Health And Human Services | Raccoon poxvirus as a gene expression and vaccine vector for genes of rabies virus and other organisms |
| DE10399031I1 (de) | 1987-08-28 | 2004-01-29 | Health Research Inc | Rekombinante Viren. |
| CA1340203C (en) | 1987-09-16 | 1998-12-15 | Noboru Yanagida | Recombinant apivoxvirus |
| US5093258A (en) | 1988-08-26 | 1992-03-03 | Therion Biologics Corporation | Recombinant fowlpox virus and recombination vector |
| WO1991019803A1 (en) | 1990-06-19 | 1991-12-26 | Applied Biotechnology, Incorporated | Self assembled, defective, nonself-propagating viral particles |
| JP3399943B2 (ja) * | 1991-05-06 | 2003-04-28 | アメリカ合衆国 | 癌胎児性抗原を発現する組換えウイルスとその使用方法 |
| US5445953A (en) | 1991-08-26 | 1995-08-29 | Immuno Aktiengesellschaft | Direct molecular cloning of a modified poxvirus genome |
| US5382425A (en) * | 1992-01-13 | 1995-01-17 | Syntro Corporation | Recombinant swinepox virus |
| US5374149A (en) | 1993-01-13 | 1994-12-20 | Computower Technologies, Corp. | Vertical storage conveyor with symmetrical motor drive system |
| EP0789774A2 (en) * | 1994-10-03 | 1997-08-20 | THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES | Enhanced immune response by introduction of cytokine gene and/or costimulatory molecule b7 gene in a recombinant virus expressing system |
| CA2201587C (en) * | 1994-10-03 | 2002-06-25 | Jeffrey Schlom | Composition comprising a recombinant virus expressing an antigen and a recombinant virus expressing an immunostimulatory molecule |
| US6001349A (en) * | 1995-02-22 | 1999-12-14 | Therion Biologics Corporation | Generation of human cytotoxic T-cells specific for carcinoma self-associated antigens and uses thereof |
| US6165460A (en) * | 1995-07-10 | 2000-12-26 | Therion Biologics Corporation | Generation of immune responses to prostate-specific antigen (PSA) |
-
1997
- 1997-07-15 JP JP50884498A patent/JP4475683B2/ja not_active Expired - Lifetime
- 1997-07-15 WO PCT/US1997/012203 patent/WO1998004727A1/en not_active Ceased
- 1997-07-15 EP EP09010415A patent/EP2112225A1/en not_active Ceased
- 1997-07-15 CA CA002261989A patent/CA2261989C/en not_active Expired - Fee Related
- 1997-07-15 EP EP97934142A patent/EP0954593A1/en not_active Withdrawn
- 1997-07-15 AU AU37268/97A patent/AU731860B2/en not_active Ceased
-
2002
- 2002-07-17 US US10/197,127 patent/US7410644B2/en not_active Expired - Fee Related
-
2009
- 2009-07-22 JP JP2009171254A patent/JP2009278997A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| AU3726897A (en) | 1998-02-20 |
| EP2112225A1 (en) | 2009-10-28 |
| WO1998004727A1 (en) | 1998-02-05 |
| CA2261989A1 (en) | 1998-02-05 |
| JP2000516459A (ja) | 2000-12-12 |
| JP2009278997A (ja) | 2009-12-03 |
| EP0954593A1 (en) | 1999-11-10 |
| US7410644B2 (en) | 2008-08-12 |
| US20030003079A1 (en) | 2003-01-02 |
| JP4475683B2 (ja) | 2010-06-09 |
| AU731860B2 (en) | 2001-04-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2261989C (en) | Recombinant pox virus for immunization against tumor-associated antigens | |
| US7118738B2 (en) | Recombinant pox virus for immunization against MUC1 tumor-associated antigen | |
| AU774076B2 (en) | A recombinant vector expressing multiple costimulatory molecules and uses thereof | |
| US6969609B1 (en) | Recombinant vector expressing multiple costimulatory molecules and uses thereof | |
| AU2004289340B2 (en) | System for treating and preventing breast cancer | |
| AU718945B2 (en) | Recombinant pox virus for immunization against tumor-associated antigens | |
| CA2252406A1 (en) | Heterologous boosting immunizations | |
| US20050100558A1 (en) | Heterologous boosting immunizations | |
| AU767562B2 (en) | Recombinant pox virus for immunization against tumor-associated antigens | |
| AU2003266471A1 (en) | Recombinant pox virus for immunization against tumor-associated antigens | |
| EP1865065A1 (en) | A recombinant vector expressing multiple costimulatory molecules and uses thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |
Effective date: 20170717 |