CA2250857A1 - Ribozymes asymetriques en forme de tete de marteau - Google Patents

Ribozymes asymetriques en forme de tete de marteau Download PDF

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Publication number
CA2250857A1
CA2250857A1 CA 2250857 CA2250857A CA2250857A1 CA 2250857 A1 CA2250857 A1 CA 2250857A1 CA 2250857 CA2250857 CA 2250857 CA 2250857 A CA2250857 A CA 2250857A CA 2250857 A1 CA2250857 A1 CA 2250857A1
Authority
CA
Canada
Prior art keywords
compound
host cell
ribozyme
nucleotides
cleavage
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA 2250857
Other languages
English (en)
Inventor
Philip Hendry
Maxine J. Mccall
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Commonwealth Scientific and Industrial Research Organization CSIRO
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2250857A1 publication Critical patent/CA2250857A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C12N2310/121—Hammerhead
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/321—2'-O-R Modification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

L'invention concerne un composé ayant la formule (I). Dans cette formule, N peut être un nucléotide, n est égal à 1-5 et n' est égal à 1-3. Ces ribozymes asymétriques présentent des vitesses de scission plus élevées que la normale. Le composé peut être lié par covalence à un agent d'administration. L'invention concerne, en outre, une composition qui comprend le composé en association avec un vecteur acceptable. L'invention concerne également une méthode pour scinder une séquence cible d'ARN avec le composé décrit ci-dessus. L'invention concerne, également, une méthode pour traiter une maladie associée à un ARN particulier, chez un humain ou chez un animal, qui consiste à administrer le composé à cet humain ou à cet animal. En outre, l'invention concerne un réactif de diagnostic qui comprend le composé.
CA 2250857 1996-04-02 1997-04-02 Ribozymes asymetriques en forme de tete de marteau Abandoned CA2250857A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US62703396A 1996-04-02 1996-04-02
US1465996P 1996-04-02 1996-04-02
US08/627,033 1996-04-02
US60/014,659 1996-04-02

Publications (1)

Publication Number Publication Date
CA2250857A1 true CA2250857A1 (fr) 1997-10-09

Family

ID=26686341

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2250857 Abandoned CA2250857A1 (fr) 1996-04-02 1997-04-02 Ribozymes asymetriques en forme de tete de marteau

Country Status (5)

Country Link
EP (1) EP0902836A4 (fr)
JP (1) JP2000509969A (fr)
AU (1) AU721758B2 (fr)
CA (1) CA2250857A1 (fr)
WO (1) WO1997037013A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004512810A (ja) * 1999-08-31 2004-04-30 サーナ・セラピューティクス・インコーポレイテッド 核酸に基づく遺伝子発現の調節剤
WO2001030362A2 (fr) * 1999-10-26 2001-05-03 Immusol Incorporated Therapie ribozymique destinee au traitement de maladies proliferantes de la peau et des yeux
GB0224663D0 (en) * 2002-10-23 2002-12-04 Isis Innovation DNAzyme

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991019789A1 (fr) * 1990-06-19 1991-12-26 Commonwealth Scientific And Industrial Research Organisation Endonucleases
WO1993023569A1 (fr) * 1992-05-11 1993-11-25 Ribozyme Pharmaceuticals, Inc. Procede et reactif d'inhibition de la replication virale
EP0641212A4 (fr) * 1992-05-14 1997-05-21 Ribozyme Pharm Inc Procede et reactif destines a empecher l'evolution du cancer.
JPH07509133A (ja) * 1992-07-17 1995-10-12 リボザイム・ファーマシューティカルズ・インコーポレイテッド 動物疾患の処置のための方法および剤
AU5334294A (en) * 1993-10-15 1995-05-04 Dkfz Deutsches Krebsforschungszentrum Stiftung Des Offentlichen Rechts Asymmetric hammerhead ribozymes and nucleotide sequences for their construction

Also Published As

Publication number Publication date
EP0902836A4 (fr) 2004-12-22
AU721758B2 (en) 2000-07-13
WO1997037013A1 (fr) 1997-10-09
EP0902836A1 (fr) 1999-03-24
JP2000509969A (ja) 2000-08-08
AU2145097A (en) 1997-10-22

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Legal Events

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EEER Examination request
FZDE Dead