CA2245666C - Synthese en phase solide de phosphoramidates n3'.fwdarw.p5' oligonucleotidiques - Google Patents

Synthese en phase solide de phosphoramidates n3'.fwdarw.p5' oligonucleotidiques Download PDF

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Publication number
CA2245666C
CA2245666C CA 2245666 CA2245666A CA2245666C CA 2245666 C CA2245666 C CA 2245666C CA 2245666 CA2245666 CA 2245666 CA 2245666 A CA2245666 A CA 2245666A CA 2245666 C CA2245666 C CA 2245666C
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Canada
Prior art keywords
phosphoramidite
amino
fwdarw
monomer
group
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA 2245666
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English (en)
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CA2245666A1 (fr
Inventor
Bernard L. Hirschbein
Karen L. Fearon
Sergei M. Gryaznov
Sarah N. Mccurdy
Jeffrey S. Nelson
Ronald G. Schultz
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Geron Corp
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Geron Corp
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Publication date
Priority claimed from US08/603,566 external-priority patent/US5684143A/en
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Publication of CA2245666A1 publication Critical patent/CA2245666A1/fr
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Publication of CA2245666C publication Critical patent/CA2245666C/fr
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Expired - Lifetime legal-status Critical Current

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Abstract

L'invention concerne un procédé de synthèse de phosphoramidates N3'}P5' oligonucléotidiques par une réaction à échange d'amines de phosphoramidites au cours de laquelle un groupe 3'-amino déprotégé d'une chaîne oligonucléotidique supportée en phase solide est échangé contre la portion amino d'un 5'-phosphoramidite d'un monomère entrant qui possède un groupe 3'-amino protégé. La liaison phosphoramidite internucléotidique résultante est ensuite oxydée pour former une liaison phosphoramidate stable et protégée. Le procédé de l'invention permet d'améliorer considérablement les rendements de production et réduit l'utilisation de réactifs en comparaison aux procédés actuels pour effectuer la synthèse de la classe de composé indiquée ci-dessus.
CA 2245666 1996-02-21 1996-06-14 Synthese en phase solide de phosphoramidates n3'.fwdarw.p5' oligonucleotidiques Expired - Lifetime CA2245666C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/603,566 1996-02-21
US08/603,566 US5684143A (en) 1996-02-21 1996-02-21 Oligo-2'-fluoronucleotide N3'->P5' phosphoramidates
PCT/US1996/010418 WO1997031009A1 (fr) 1996-02-21 1996-06-14 Synthese en phase solide de phosphoramidates n3'→p5' oligonucleotidiques

Publications (2)

Publication Number Publication Date
CA2245666A1 CA2245666A1 (fr) 1997-08-28
CA2245666C true CA2245666C (fr) 2009-11-10

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Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2245666 Expired - Lifetime CA2245666C (fr) 1996-02-21 1996-06-14 Synthese en phase solide de phosphoramidates n3'.fwdarw.p5' oligonucleotidiques

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CA (1) CA2245666C (fr)

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Publication number Publication date
CA2245666A1 (fr) 1997-08-28

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